Characterization of the catalytic site of the ADP-ribosyltransferase Clostridium botulinum C2 toxin by site-directed mutagenesis.

PubWeight™: 1.15‹?› | Rank: Top 10%

🔗 View Article (PMID 9792657)

Published in J Biol Chem on November 06, 1998

Authors

H Barth1, J C Preiss, F Hofmann, K Aktories

Author Affiliations

1: Institut für Pharmakologie und Toxikologie der Albert-Ludwigs-Universität Freiburg, D-79104 Freiburg, Germany.

Articles citing this

Binary bacterial toxins: biochemistry, biology, and applications of common Clostridium and Bacillus proteins. Microbiol Mol Biol Rev (2004) 2.60

Impaired recycling of synaptic vesicles after acute perturbation of the presynaptic actin cytoskeleton. Proc Natl Acad Sci U S A (2002) 1.64

Characterization of the enzymatic component of the ADP-ribosyltransferase toxin CDTa from Clostridium difficile. Infect Immun (2001) 1.54

A Single Legionella Effector Catalyzes a Multistep Ubiquitination Pathway to Rearrange Tubular Endoplasmic Reticulum for Replication. Cell Host Microbe (2016) 1.46

Structural basis of actin recognition and arginine ADP-ribosylation by Clostridium perfringens iota-toxin. Proc Natl Acad Sci U S A (2008) 1.31

Cellular uptake of the Clostridium perfringens binary iota-toxin. Infect Immun (2001) 1.26

Characterization of the enzymatic component of Clostridium perfringens iota-toxin. J Bacteriol (2000) 1.13

The C terminus of component C2II of Clostridium botulinum C2 toxin is essential for receptor binding. Infect Immun (2000) 0.95

ADP-ribosylation of actin by the Clostridium botulinum C2 toxin in mammalian cells results in delayed caspase-dependent apoptotic cell death. Infect Immun (2008) 0.95

Clostridium perfringens iota-toxin: structure and function. Toxins (Basel) (2009) 0.93

Clostridium and bacillus binary enterotoxins: bad for the bowels, and eukaryotic being. Toxins (Basel) (2014) 0.91

Tailored ß-cyclodextrin blocks the translocation pores of binary exotoxins from C. botulinum and C. perfringens and protects cells from intoxication. PLoS One (2011) 0.89

Photox, a novel actin-targeting mono-ADP-ribosyltransferase from Photorhabdus luminescens. J Biol Chem (2010) 0.88

The long-lived nature of clostridium perfringens iota toxin in mammalian cells induces delayed apoptosis. Infect Immun (2009) 0.86

Clostridial binary toxins: iota and C2 family portraits. Front Cell Infect Microbiol (2011) 0.86

Certhrax toxin, an anthrax-related ADP-ribosyltransferase from Bacillus cereus. J Biol Chem (2012) 0.82

Interactions of high-affinity cationic blockers with the translocation pores of B. anthracis, C. botulinum, and C. perfringens binary toxins. Biophys J (2012) 0.82

A recombinant fusion toxin based on enzymatic inactive C3bot1 selectively targets macrophages. PLoS One (2013) 0.81

Cationic PAMAM dendrimers as pore-blocking binary toxin inhibitors. Biomacromolecules (2014) 0.78

EGA Protects Mammalian Cells from Clostridium difficile CDT, Clostridium perfringens Iota Toxin and Clostridium botulinum C2 Toxin. Toxins (Basel) (2016) 0.77

A novel Hsp70 inhibitor prevents cell intoxication with the actin ADP-ribosylating Clostridium perfringens iota toxin. Sci Rep (2016) 0.76

Retargeting the Clostridium botulinum C2 toxin to the neuronal cytosol. Sci Rep (2016) 0.75

Molecular Evolutionary Constraints that Determine the Avirulence State of Clostridium botulinum C2 Toxin. J Mol Evol (2017) 0.75

Functional significance of active site residues in the enzymatic component of the Clostridium difficile binary toxin. Biochem Biophys Rep (2016) 0.75

Articles by these authors

Glucosylation of Rho proteins by Clostridium difficile toxin B. Nature (1995) 6.08

Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice. Nature (2003) 5.84

A family of hyperpolarization-activated mammalian cation channels. Nature (1998) 4.97

Microinjection of recombinant p21rho induces rapid changes in cell morphology. J Cell Biol (1990) 4.83

Sustained signaling leading to T cell activation results from prolonged T cell receptor occupancy. Role of T cell actin cytoskeleton. J Exp Med (1995) 4.57

The naming of voltage-gated calcium channels. Neuron (1994) 4.31

Primary structure and functional expression from complementary DNA of a brain calcium channel. Nature (1991) 3.77

Gln 63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1. Nature (1997) 3.53

Botulinum C2 toxin ADP-ribosylates actin. Nature (1986) 3.16

Nomenclature of voltage-gated sodium channels. Neuron (2000) 3.10

Nomenclature of voltage-gated calcium channels. Neuron (2000) 3.09

The enterotoxin from Clostridium difficile (ToxA) monoglucosylates the Rho proteins. J Biol Chem (1995) 3.02

PTK787/ZK 222584, a novel and potent inhibitor of vascular endothelial growth factor receptor tyrosine kinases, impairs vascular endothelial growth factor-induced responses and tumor growth after oral administration. Cancer Res (2000) 3.02

Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II. Science (1996) 3.01

The roles of the subunits in the function of the calcium channel. Science (1991) 2.97

Cerebellar ataxia and Purkinje cell dysfunction caused by Ca2+-activated K+ channel deficiency. Proc Natl Acad Sci U S A (2004) 2.97

Defective smooth muscle regulation in cGMP kinase I-deficient mice. EMBO J (1998) 2.96

Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice. Nat Cell Biol (2001) 2.94

On the mechanism of beta-adrenergic regulation of the Ca channel in the guinea-pig heart. Pflugers Arch (1985) 2.93

Serum antibodies to L-type calcium channels in patients with amyotrophic lateral sclerosis. N Engl J Med (1992) 2.86

The N-terminal domain of Pseudomonas aeruginosa exoenzyme S is a GTPase-activating protein for Rho GTPases. J Biol Chem (1999) 2.78

Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O. Proc Natl Acad Sci U S A (2001) 2.67

GAP activity of the Yersinia YopE cytotoxin specifically targets the Rho pathway: a mechanism for disruption of actin microfilament structure. Mol Microbiol (2000) 2.56

Two pacemaker channels from human heart with profoundly different activation kinetics. EMBO J (1999) 2.51

Cellular expression and functional characterization of four hyperpolarization-activated pacemaker channels in cardiac and neuronal tissues. Eur J Biochem (2001) 2.47

Selective loss of cone function in mice lacking the cyclic nucleotide-gated channel CNG3. Proc Natl Acad Sci U S A (1999) 2.46

New views on RPE65 deficiency: the rod system is the source of vision in a mouse model of Leber congenital amaurosis. Nat Genet (2001) 2.44

Purified dihydropyridine-binding site from skeletal muscle t-tubules is a functional calcium channel. Nature (1986) 2.41

Blockade of vascular endothelial cell growth factor receptor signaling is sufficient to completely prevent retinal neovascularization. Am J Pathol (2000) 2.41

Primary structure of the beta subunit of the DHP-sensitive calcium channel from skeletal muscle. Science (1989) 2.40

Injection of subunits of cyclic AMP-dependent protein kinase into cardiac myocytes modulates Ca2+ current. Nature (1982) 2.33

Deamidation of Cdc42 and Rac by Escherichia coli cytotoxic necrotizing factor 1: activation of c-Jun N-terminal kinase in HeLa cells. Infect Immun (1999) 2.26

Modulation of Ca current during the phosphorylation cycle in the guinea pig heart. Pflugers Arch (1986) 2.26

Regulation of intracellular calcium by a signalling complex of IRAG, IP3 receptor and cGMP kinase Ibeta. Nature (2000) 2.17

Functional embryonic cardiomyocytes after disruption of the L-type alpha1C (Cav1.2) calcium channel gene in the mouse. J Biol Chem (2000) 2.09

Normalization of current kinetics by interaction between the alpha 1 and beta subunits of the skeletal muscle dihydropyridine-sensitive Ca2+ channel. Nature (1991) 2.09

Clostridium difficile toxin B acts on the GTP-binding protein Rho. J Biol Chem (1994) 2.09

Primary structure and functional expression of a high voltage activated calcium channel from rabbit lung. FEBS Lett (1990) 2.08

A common motif of eukaryotic glycosyltransferases is essential for the enzyme activity of large clostridial cytotoxins. J Biol Chem (1998) 2.08

Structure and functional expression of a new member of the tetrodotoxin-sensitive voltage-activated sodium channel family from human neuroendocrine cells. EMBO J (1995) 2.06

Regional expression and cellular localization of the alpha1 and beta subunit of high voltage-activated calcium channels in rat brain. J Neurosci (1997) 2.05

Comparison of adenosine 3':5'-monophosphate-dependent protein kinases from rabbit skeletal and bovine heart muscle. J Biol Chem (1975) 2.03

Temporally controlled somatic mutagenesis in smooth muscle. Genesis (2000) 2.01

Ephrin-A5 induces collapse of growth cones by activating Rho and Rho kinase. J Cell Biol (2000) 1.94

Calcium channel beta subunit heterogeneity: functional expression of cloned cDNA from heart, aorta and brain. EMBO J (1992) 1.92

Clostridium perfringens iota toxin ADP-ribosylates skeletal muscle actin in Arg-177. FEBS Lett (1987) 1.85

Localization of the glucosyltransferase activity of Clostridium difficile toxin B to the N-terminal part of the holotoxin. J Biol Chem (1997) 1.83

Differential distribution of four hyperpolarization-activated cation channels in mouse brain. Biol Chem (1999) 1.82

The amino-terminal domain of Pseudomonas aeruginosa ExoS disrupts actin filaments via small-molecular-weight GTP-binding proteins. Mol Microbiol (1999) 1.81

Pseudomonas aeruginosa ExoT is a Rho GTPase-activating protein. Infect Immun (2000) 1.78

Transmission of hepatitis C virus from a patient to an anesthesiology assistant to five patients. N Engl J Med (2000) 1.67

Concentrations of cyclic AMP-dependent protein kinase subunits in various tissues. J Biol Chem (1977) 1.67

Molecular diversity of the calcium channel alpha2delta subunit. J Neurosci (1999) 1.63

Structure and function of cyclic nucleotide-gated channels. Rev Physiol Biochem Pharmacol (1999) 1.59

An isoform of the rod photoreceptor cyclic nucleotide-gated channel beta subunit expressed in olfactory neurons. Proc Natl Acad Sci U S A (1998) 1.57

Soluble guanylate cyclase purified from bovine lung contains heme and copper. FEBS Lett (1981) 1.55

Cellular uptake of Clostridium botulinum C2 toxin requires oligomerization and acidification. J Biol Chem (2000) 1.54

Characterization of the enzymatic component of the ADP-ribosyltransferase toxin CDTa from Clostridium difficile. Infect Immun (2001) 1.54

The low molecular mass GTP-binding protein Rho is affected by toxin A from Clostridium difficile. J Clin Invest (1995) 1.51

Phosphorylation of the type-II regulatory subunit of cyclic-AMP-dependent protein kinase by glycogen synthase kinase 3 and glycogen synthase kinase 5. Eur J Biochem (1982) 1.50

Amyotrophic lateral sclerosis patient antibodies label Ca2+ channel alpha 1 subunit. Ann Neurol (1994) 1.50

Inactivation of Ras by Clostridium sordellii lethal toxin-catalyzed glucosylation. J Biol Chem (1996) 1.50

Cyclic GMP-dependent protein kinase stimulates the plasmalemmal Ca2+ pump of smooth muscle via phosphorylation of phosphatidylinositol. Biochem J (1988) 1.50

Low pH-induced formation of ion channels by clostridium difficile toxin B in target cells. J Biol Chem (2001) 1.49

The Rho-deamidating cytotoxic necrotizing factor 1 from Escherichia coli possesses transglutaminase activity. Cysteine 866 and histidine 881 are essential for enzyme activity. J Biol Chem (1998) 1.46

Bacterial protein toxins targeting rho GTPases. FEMS Microbiol Lett (2000) 1.46

[Irritable bowel syndrome: German consensus guidelines on definition, pathophysiology and management]. Z Gastroenterol (2011) 1.46

Purification and characterization of an ADP-ribosyltransferase produced by Clostridium limosum. J Biol Chem (1992) 1.45

Differential and age-dependent expression of hyperpolarization-activated, cyclic nucleotide-gated cation channel isoforms 1-4 suggests evolving roles in the developing rat hippocampus. Neuroscience (2001) 1.44

Clostridium botulinum type C produces a novel ADP-ribosyltransferase distinct from botulinum C2 toxin. FEBS Lett (1987) 1.44

Glucosylation and ADP ribosylation of rho proteins: effects on nucleotide binding, GTPase activity, and effector coupling. Biochemistry (1998) 1.44

Another member of the cyclic nucleotide-gated channel family, expressed in testis, kidney, and heart. Proc Natl Acad Sci U S A (1994) 1.44

Botulinum ADP-ribosyltransferase C3: a new tool to study low molecular weight GTP-binding proteins. Trends Pharmacol Sci (1989) 1.42

Requirement of Rac activity for maintenance of capillary endothelial barrier properties. Am J Physiol Heart Circ Physiol (2003) 1.41

Alternatively spliced IS6 segments of the alpha 1C gene determine the tissue-specific dihydropyridine sensitivity of cardiac and vascular smooth muscle L-type Ca2+ channels. Circ Res (1997) 1.41

Purification of the 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 from porcine brain cytosol and its characterization as a GTP-binding protein highly homologous to the rho gene product. FEBS Lett (1989) 1.41

A family of gamma-like calcium channel subunits. FEBS Lett (2000) 1.39

The cDNA of the two isoforms of bovine cGMP-dependent protein kinase. FEBS Lett (1989) 1.39

Transduction in taste receptor cells requires cAMP-dependent protein kinase. Nature (1988) 1.39

Cyclic GMP-dependent protein kinase phosphorylates phospholamban in isolated sarcoplasmic reticulum from cardiac and smooth muscle. Biochem J (1988) 1.38

Botulinum C2 toxin ADP-ribosylates actin and disorganizes the microfilament network in intact cells. Eur J Cell Biol (1987) 1.37

Erectile dysfunction in cyclic GMP-dependent kinase I-deficient mice. Proc Natl Acad Sci U S A (2000) 1.36

Mechanisms of NO/cGMP-dependent vasorelaxation. Circ Res (2000) 1.36

Involvement of the GTP binding protein Rho in constitutive endocytosis in Xenopus laevis oocytes. J Cell Biol (1995) 1.36

Absence of the gamma subunit of the skeletal muscle dihydropyridine receptor increases L-type Ca2+ currents and alters channel inactivation properties. J Biol Chem (2000) 1.35

Three amino acids in the C-linker are major determinants of gating in cyclic nucleotide-gated channels. EMBO J (1998) 1.35

Structure and function of cGMP-dependent protein kinases. Rev Physiol Biochem Pharmacol (1999) 1.34

Dramatic inhibition of retinal and choroidal neovascularization by oral administration of a kinase inhibitor. Am J Pathol (1999) 1.34

Calcium channel alpha(2)delta subunits-structure and Gabapentin binding. Mol Pharmacol (2001) 1.33

Biological activity of a C-terminal fragment of Pasteurella multocida toxin. Infect Immun (2001) 1.33

Uncoupling of alpha-adrenoceptor-mediated inhibition of human platelet adenylate cyclase by N-ethylmaleimide. J Biol Chem (1982) 1.32

Role of Rho proteins in carbachol-induced contractions in intact and permeabilized guinea-pig intestinal smooth muscle. J Physiol (1996) 1.32

Effect of disruption of actin filaments by Clostridium botulinum C2 toxin on insulin secretion in HIT-T15 cells and pancreatic islets. Mol Biol Cell (1994) 1.32

Demonstration of cGMP-dependent protein kinase and cGMP-dependent phosphorylation in cell-free extracts of platelets. Eur J Biochem (1986) 1.31

Calmodulin is essential for smooth muscle contraction. FEBS Lett (1981) 1.31