Top articles

Rank Title Journal Year PubWeight™‹?›
1 A short history of SHELX. Acta Crystallogr A 2007 7339.18
2 Protein measurement with the Folin phenol reagent. J Biol Chem 1951 1743.91
3 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970 1528.65
4 Structure validation in chemical crystallography. Acta Crystallogr D Biol Crystallogr 2009 1216.15
5 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976 903.81
6 DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 1977 790.54
7 Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997 665.31
8 Basic local alignment search tool. J Mol Biol 1990 659.07
9 Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975 503.08
10 Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001 414.27
11 CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994 392.47
12 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 1979 365.71
13 Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 2000 336.52
14 "Mini-mental state". A practical method for grading the cognitive state of patients for the clinician. J Psychiatr Res 1975 301.74
15 Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 1980 287.68
16 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987 281.19
17 The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987 266.90
18 A new method for sequencing DNA. Proc Natl Acad Sci U S A 1977 250.51
19 Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 2009 235.12
20 The Cambridge Structural Database: a quarter of a million crystal structures and rising. Acta Crystallogr B 2002 234.73
21 The Sequence Alignment/Map format and SAMtools. Bioinformatics 2009 232.39
22 Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 2004 227.01
23 MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011 220.97
24 Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988 220.77
25 The measurement of observer agreement for categorical data. Biometrics 1977 216.56
26 A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979 214.95
27 Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 1985 213.67
28 Initial sequencing and analysis of the human genome. Nature 2001 212.86
29 Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986 211.50
30 PLINK: a tool set for whole-genome association and population-based linkage analyses. Am J Hum Genet 2007 209.92
31 A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983 206.01
32 Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 1977 205.11
33 MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004 198.59
34 Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 1988 193.60
35 Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A 1998 192.97
36 Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 2009 190.94
37 The CCP4 suite: programs for protein crystallography. Acta Crystallogr D Biol Crystallogr 1994 187.88
38 The Protein Data Bank. Nucleic Acids Res 2000 187.10
39 Global cancer statistics. CA Cancer J Clin 2011 185.92
40 The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 1963 185.56
41 A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 1984 181.98
42 Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 1979 180.95
43 The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997 175.36
44 The RAST Server: rapid annotations using subsystems technology. BMC Genomics 2008 175.18
45 A new method of classifying prognostic comorbidity in longitudinal studies: development and validation. J Chronic Dis 1987 174.79
47 A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957 173.99
48 A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959 173.74
49 Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr 1998 169.28
50 MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004 168.89
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