A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14.

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Published in EMBO J on September 17, 2001

Authors

A Borodovsky1, B M Kessler, R Casagrande, H S Overkleeft, K D Wilkinson, H L Ploegh

Author Affiliations

1: Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

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ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation. Proc Natl Acad Sci U S A (1980) 2.96

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Vinyl sulfones as mechanism-based cysteine protease inhibitors. J Med Chem (1995) 2.70

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Interaction of the Doa4 deubiquitinating enzyme with the yeast 26S proteasome. Mol Biol Cell (1999) 2.57

Structural and serological similarity of MHC-linked LMP and proteasome (multicatalytic proteinase) complexes. Nature (1991) 2.56

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Purification, cDNA-cloning and expression of human diacylglycerol kinase. FEBS Lett (1990) 1.45

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Proteolysis in MHC class II antigen presentation: who's in charge? Immunity (2000) 1.32

Mechanisms of induction of primary virus-specific cytotoxic T lymphocyte responses. Eur J Immunol (1992) 1.30

Why certain antibodies cross-react with HLA-A and HLA-G: epitope mapping of two common MHC class I reagents. Mol Immunol (1998) 1.29

Altered natural killer cell repertoire in Tap-1 mutant mice. Proc Natl Acad Sci U S A (1994) 1.28

Degradation of mouse invariant chain: roles of cathepsins S and D and the influence of major histocompatibility complex polymorphism. J Exp Med (1997) 1.25

Involvement of the vacuolar H(+)-ATPases in the secretory pathway of HepG2 cells. J Biol Chem (1993) 1.25

Methodology employed for anaerobic spectrophotometric titrations and for computer-assisted data analysis. Methods Enzymol (1979) 1.25

Intracellular transport of MHC class II molecules. Immunol Today (1992) 1.24