Published in Nature on April 29, 2009
Genome editing with engineered zinc finger nucleases. Nat Rev Genet (2010) 18.88
Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol (2009) 12.40
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol (2013) 11.58
A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity. Nucleic Acids Res (2011) 7.93
TALENs: a widely applicable technology for targeted genome editing. Nat Rev Mol Cell Biol (2012) 7.01
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures. Nat Methods (2010) 6.00
High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. Proc Natl Acad Sci U S A (2010) 4.78
Genome engineering with zinc-finger nucleases. Genetics (2011) 4.66
ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool. Nucleic Acids Res (2010) 3.59
Targeted genome modification in mice using zinc-finger nucleases. Genetics (2010) 3.38
Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Nat Protoc (2009) 2.67
TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nat Methods (2013) 2.64
Transcription activator-like effector nucleases enable efficient plant genome engineering. Plant Physiol (2012) 2.36
Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme. Genome Res (2012) 2.02
Advances in targeted genome editing. Curr Opin Chem Biol (2012) 1.97
Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion. Nucleic Acids Res (2010) 1.92
The Regulatory Status of Genome-edited Crops. Plant Biotechnol J (2015) 1.91
Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy. Curr Gene Ther (2011) 1.76
Precision genome engineering and agriculture: opportunities and regulatory challenges. PLoS Biol (2014) 1.67
Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases. Proc Natl Acad Sci U S A (2010) 1.65
Deployment of new biotechnologies in plant breeding. Nat Biotechnol (2012) 1.64
Renegotiating GM crop regulation. Targeted gene-modification technology raises new issues for the oversight of genetically modified crops. EMBO Rep (2011) 1.63
Noncoding RNA gene silencing through genomic integration of RNA destabilizing elements using zinc finger nucleases. Genome Res (2011) 1.59
DNA replicons for plant genome engineering. Plant Cell (2014) 1.57
Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA. Plant Physiol (2015) 1.39
Recent progress of flower colour modification by biotechnology. Int J Mol Sci (2009) 1.37
Zinc finger nucleases as tools to understand and treat human diseases. BMC Med (2010) 1.36
Genome editing in rice and wheat using the CRISPR/Cas system. Nat Protoc (2014) 1.33
Targeted mutagenesis of duplicated genes in soybean with zinc-finger nucleases. Plant Physiol (2011) 1.29
Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnol (2015) 1.28
A proposed regulatory framework for genome-edited crops. Nat Genet (2016) 1.27
Sequence specificity is obtained from the majority of modular C2H2 zinc-finger arrays. Nucleic Acids Res (2011) 1.27
Targeted genome editing of sweet orange using Cas9/sgRNA. PLoS One (2014) 1.26
In planta gene targeting. Proc Natl Acad Sci U S A (2012) 1.25
A time-invariant principle of genome evolution. Proc Natl Acad Sci U S A (2010) 1.24
Cas9-Guide RNA Directed Genome Editing in Soybean. Plant Physiol (2015) 1.18
Adding fingers to an engineered zinc finger nuclease can reduce activity. Biochemistry (2011) 1.17
Targeted manipulation of mammalian genomes using designed zinc finger nucleases. Biochem Biophys Res Commun (2009) 1.15
Generation of redesigned homing endonucleases comprising DNA-binding domains derived from two different scaffolds. Nucleic Acids Res (2009) 1.13
ZFN-mediated gene targeting of the Arabidopsis protoporphyrinogen oxidase gene through Agrobacterium-mediated floral dip transformation. Plant Biotechnol J (2012) 1.12
Inositol 1,3,4,5,6-pentakisphosphate 2-kinase is a distant IPK member with a singular inositide binding site for axial 2-OH recognition. Proc Natl Acad Sci U S A (2010) 1.10
Chromosomal context and epigenetic mechanisms control the efficacy of genome editing by rare-cutting designer endonucleases. Nucleic Acids Res (2012) 1.09
Generation of improved humanized mouse models for human infectious diseases. J Immunol Methods (2014) 1.08
Nontransgenic genome modification in plant cells. Plant Physiol (2010) 1.08
Newer gene editing technologies toward HIV gene therapy. Viruses (2013) 1.07
p53 Gene repair with zinc finger nucleases optimised by yeast 1-hybrid and validated by Solexa sequencing. PLoS One (2011) 1.04
High-frequency, precise modification of the tomato genome. Genome Biol (2015) 1.04
Advanced genetic tools for plant biotechnology. Nat Rev Genet (2013) 1.04
Gene correction by homologous recombination with zinc finger nucleases in primary cells from a mouse model of a generic recessive genetic disease. Mol Ther (2010) 1.04
Evolutionary and comparative analyses of the soybean genome. Breed Sci (2012) 1.02
Engineering RNA endonucleases with customized sequence specificities. Nat Commun (2012) 1.01
Creating designed zinc-finger nucleases with minimal cytotoxicity. J Mol Biol (2010) 1.00
Elucidation of the structure and reaction mechanism of sorghum hydroxycinnamoyltransferase and its structural relationship to other coenzyme a-dependent transferases and synthases. Plant Physiol (2013) 0.99
Targeted molecular trait stacking in cotton through targeted double-strand break induction. Plant Biotechnol J (2013) 0.99
Application of gene targeting to designed mutation breeding of high-tryptophan rice. Plant Physiol (2011) 0.98
Transgenics: A new breed. Nature (2013) 0.98
Gene editing of human embryonic stem cells via an engineered baculoviral vector carrying zinc-finger nucleases. Mol Ther (2011) 0.97
Predicting success of oligomerized pool engineering (OPEN) for zinc finger target site sequences. BMC Bioinformatics (2010) 0.96
CCR5 Gene Editing of Resting CD4(+) T Cells by Transient ZFN Expression From HIV Envelope Pseudotyped Nonintegrating Lentivirus Confers HIV-1 Resistance in Humanized Mice. Mol Ther Nucleic Acids (2014) 0.96
Less is more: strategies to remove marker genes from transgenic plants. BMC Biotechnol (2013) 0.96
Genomics reveals new landscapes for crop improvement. Genome Biol (2013) 0.93
Targeted genome modification technologies and their applications in crop improvements. Plant Cell Rep (2013) 0.93
Activities and specificities of homodimeric TALENs in Saccharomyces cerevisiae. Curr Genet (2013) 0.92
A novel zinc-finger nuclease platform with a sequence-specific cleavage module. Nucleic Acids Res (2011) 0.92
Characterization and DNA-binding specificities of Ralstonia TAL-like effectors. Mol Plant (2013) 0.92
Targeted deletion and inversion of tandemly arrayed genes in Arabidopsis thaliana using zinc finger nucleases. G3 (Bethesda) (2013) 0.92
Powdery mildew resistance in tomato by impairment of SlPMR4 and SlDMR1. PLoS One (2013) 0.91
Native homing endonucleases can target conserved genes in humans and in animal models. Nucleic Acids Res (2011) 0.90
Stacking multiple transgenes at a selected genomic site via repeated recombinase-mediated DNA cassette exchanges. Plant Physiol (2010) 0.90
Analysis of illegitimate genomic integration mediated by zinc-finger nucleases: implications for specificity of targeted gene correction. BMC Mol Biol (2010) 0.89
Nutritionally enhanced food crops; progress and perspectives. Int J Mol Sci (2015) 0.89
Nonhomologous end joining-mediated gene replacement in plant cells. Plant Physiol (2013) 0.88
Probing the DNA-binding affinity and specificity of designed zinc finger proteins. Biophys J (2010) 0.88
Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System. PLoS One (2015) 0.88
Systems and synthetic biology approaches to alter plant cell walls and reduce biomass recalcitrance. Plant Biotechnol J (2014) 0.87
Epigenetic control of Agrobacterium T-DNA integration. Biochim Biophys Acta (2011) 0.86
Emerging gene editing strategies for Duchenne muscular dystrophy targeting stem cells. Front Physiol (2014) 0.86
Genome-editing technologies and their potential application in horticultural crop breeding. Hortic Res (2015) 0.86
Genetic and genomic toolbox of Zea mays. Genetics (2015) 0.86
Progress of cereal transformation technology mediated by Agrobacterium tumefaciens. Front Plant Sci (2014) 0.85
Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos. Proc Natl Acad Sci U S A (2012) 0.84
Origins of Programmable Nucleases for Genome Engineering. J Mol Biol (2015) 0.83
Targeted Modification of Gene Function Exploiting Homology-Directed Repair of TALEN-Mediated Double-Strand Breaks in Barley. G3 (Bethesda) (2015) 0.83
ZFNGenome: a comprehensive resource for locating zinc finger nuclease target sites in model organisms. BMC Genomics (2011) 0.83
Precision genetic modifications: a new era in molecular biology and crop improvement. Planta (2014) 0.83
Zinc finger nuclease and homing endonuclease-mediated assembly of multigene plant transformation vectors. Plant Physiol (2011) 0.83
Genome editing for crop improvement: Challenges and opportunities. GM Crops Food (2015) 0.82
Positive-negative-selection-mediated gene targeting in rice. Front Plant Sci (2015) 0.81
Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease. BMC Biotechnol (2012) 0.81
ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions. Plant Biotechnol J (2016) 0.81
New biotechnology enhances the application of cisgenesis in plant breeding. Front Plant Sci (2014) 0.81
DNA damage and repair in plants - from models to crops. Front Plant Sci (2015) 0.80
Homology-based double-strand break-induced genome engineering in plants. Plant Cell Rep (2016) 0.80
Responses to environmental stresses in woody plants: key to survive and longevity. J Plant Res (2011) 0.80
Biotechnological strategies and tools for Plum pox virus resistance: trans-, intra-, cis-genesis, and beyond. Front Plant Sci (2015) 0.80
The brown algae Pl.LSU/2 group II intron-encoded protein has functional reverse transcriptase and maturase activities. PLoS One (2013) 0.80
Maize transformation technology development for commercial event generation. Front Plant Sci (2014) 0.80
NEATTILL: A simplified procedure for nucleic acid extraction from arrayed tissue for TILLING and other high-throughput reverse genetic applications. Plant Methods (2010) 0.80
Activity and specificity of TRV-mediated gene editing in plants. Plant Signal Behav (2015) 0.80
Biallelic Gene Targeting in Rice. Plant Physiol (2015) 0.79
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods (2001) 414.27
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J (1985) 20.39
Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature (2005) 19.15
An improved zinc-finger nuclease architecture for highly specific genome editing. Nat Biotechnol (2007) 17.80
Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A. Science (1991) 15.97
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat Biotechnol (2008) 14.75
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat Biotechnol (2008) 14.20
Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Proc Natl Acad Sci U S A (1996) 12.25
Chimeric nucleases stimulate gene targeting in human cells. Science (2003) 12.12
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell (2008) 11.24
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery. Nat Biotechnol (2007) 9.23
Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics (2002) 9.09
Design and selection of novel Cys2His2 zinc finger proteins. Annu Rev Biochem (2001) 7.79
Progress and prospects: zinc-finger nucleases as gene therapy agents. Gene Ther (2008) 7.28
Stimulation of homologous recombination through targeted cleavage by chimeric nucleases. Mol Cell Biol (2001) 6.68
Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases. Proc Natl Acad Sci U S A (2007) 5.93
Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol Biol (1992) 5.45
Genetic manipulation of genomes with rare-cutting endonucleases. Trends Genet (1996) 5.07
Targeted mutagenesis using zinc-finger nucleases in Arabidopsis. Proc Natl Acad Sci U S A (2005) 4.41
Stable antibody expression at therapeutic levels using the 2A peptide. Nat Biotechnol (2005) 4.14
Physical and genetic structure of the maize genome reflects its complex evolutionary history. PLoS Genet (2007) 3.66
High-frequency homologous recombination in plants mediated by zinc-finger nucleases. Plant J (2005) 3.45
A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter. Nat Biotechnol (2001) 3.12
Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. Plant Cell (1990) 3.09
Isolation of an efficient actin promoter for use in rice transformation. Plant Cell (1990) 2.94
Mammalian gene targeting with designed zinc finger nucleases. Mol Ther (2005) 2.62
Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system. Plant Physiol (2002) 2.43
Targeted transgene integration in plant cells using designed zinc finger nucleases. Plant Mol Biol (2008) 2.40
The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution. J Exp Bot (2004) 2.27
myo-Inositol-1,2,3,4,5,6-hexakisphosphate. Phytochemistry (2003) 2.01
Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum. Gene (1988) 1.92
Generation of phytate-free seeds in Arabidopsis through disruption of inositol polyphosphate kinases. Proc Natl Acad Sci U S A (2005) 1.68
Seeds for a better future: 'low phytate' grains help to overcome malnutrition and reduce pollution. Trends Plant Sci (2001) 1.49
Rapid, high-throughput engineering of sequence-specific zinc finger DNA-binding proteins. Methods Enzymol (2001) 1.49
Gene replacement by homologous recombination in plants. Plant Mol Biol (2002) 1.21
Modification of endogenous natural genes by gene targeting in rice and other higher plants. Plant Mol Biol (2005) 1.16
Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from maize: molecular and biochemical characterization. Plant Physiol (2007) 1.09
The sequence of the zein regulatory gene opaque-2 (O2) of Zea Mays. Nucleic Acids Res (1989) 0.98
Structural distinction among inositol phosphate isomers using high-energy and low-energy collisional-activated dissociation tandem mass spectrometry with electrospray ionization. J Mass Spectrom (2003) 0.85
A TALE nuclease architecture for efficient genome editing. Nat Biotechnol (2010) 26.47
Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature (2005) 19.15
Genome editing with engineered zinc finger nucleases. Nat Rev Genet (2010) 18.88
An improved zinc-finger nuclease architecture for highly specific genome editing. Nat Biotechnol (2007) 17.80
Sequence and structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding. Genome Res (2009) 15.15
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat Biotechnol (2008) 14.75
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat Biotechnol (2008) 14.20
Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol (2009) 12.40
Knockout rats via embryo microinjection of zinc-finger nucleases. Science (2009) 12.33
Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol (2011) 11.76
Targeted genome editing across species using ZFNs and TALENs. Science (2011) 9.84
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery. Nat Biotechnol (2007) 9.23
Distinct factors control histone variant H3.3 localization at specific genomic regions. Cell (2010) 8.79
Membrane transporters in drug development. Nat Rev Drug Discov (2010) 8.56
Knockout rats generated by embryo microinjection of TALENs. Nat Biotechnol (2011) 8.45
Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV. N Engl J Med (2014) 7.64
A rapid and general assay for monitoring endogenous gene modification. Methods Mol Biol (2010) 6.77
Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A (2008) 6.59
Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations. Cell (2011) 6.56
Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo. Nat Biotechnol (2010) 6.55
An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nat Biotechnol (2011) 6.44
Essential roles of PI(3)K-p110beta in cell growth, metabolism and tumorigenesis. Nature (2008) 6.04
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures. Nat Methods (2010) 6.00
Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases. Proc Natl Acad Sci U S A (2007) 5.93
Histone deimination antagonizes arginine methylation. Cell (2004) 5.19
A map of rice genome variation reveals the origin of cultivated rice. Nature (2012) 4.92
In vivo genome editing restores haemostasis in a mouse model of haemophilia. Nature (2011) 4.81
Sequence and analysis of rice chromosome 4. Nature (2002) 4.39
Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome. Genome Res (2010) 3.94