Published in Trends Biotechnol on May 09, 2013
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Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell (2013) 5.40
Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system. Cell Rep (2013) 4.72
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods (2013) 4.61
Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol (2013) 3.84
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Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res (2013) 2.60
Pivoting the plant immune system from dissection to deployment. Science (2013) 2.59
Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy. Nat Commun (2015) 2.56
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Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus. Sci Rep (2013) 2.48
Mapping human epigenomes. Cell (2013) 2.44
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Precise and heritable genome editing in evolutionarily diverse nematodes using TALENs and CRISPR/Cas9 to engineer insertions and deletions. Genetics (2013) 2.22
Engineering adeno-associated viruses for clinical gene therapy. Nat Rev Genet (2014) 2.16
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Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods (2014) 2.04
Easy quantitative assessment of genome editing by sequence trace decomposition. Nucleic Acids Res (2014) 1.96
The Regulatory Status of Genome-edited Crops. Plant Biotechnol J (2015) 1.91
From structure to systems: high-resolution, quantitative genetic analysis of RNA polymerase II. Cell (2013) 1.87
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res (2013) 1.83
Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus. Cell Res (2014) 1.83
Interpreting the language of histone and DNA modifications. Biochim Biophys Acta (2014) 1.80
Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system. Genetics (2013) 1.72
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Chemical biology. A bump-and-hole approach to engineer controlled selectivity of BET bromodomain chemical probes. Science (2014) 1.70
Eradicating HIV-1 infection: seeking to clear a persistent pathogen. Nat Rev Microbiol (2014) 1.65
Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos. Biol Reprod (2014) 1.64
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Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res (2014) 1.59
Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice. Nucleic Acids Res (2014) 1.58
Promoterless gene targeting without nucleases ameliorates haemophilia B in mice. Nature (2014) 1.57
Accelerated homologous recombination and subsequent genome modification in Drosophila. Development (2013) 1.55
RNAi screening comes of age: improved techniques and complementary approaches. Nat Rev Mol Cell Biol (2014) 1.54
Partially redundant enhancers cooperatively maintain Mammalian pomc expression above a critical functional threshold. PLoS Genet (2015) 1.53
Potential of promotion of alleles by genome editing to improve quantitative traits in livestock breeding programs. Genet Sel Evol (2015) 1.52
The CRISPR/Cas system mediates efficient genome engineering in Bombyx mori. Cell Res (2013) 1.52
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Cell Rep (2014) 1.50
Fatal autoimmunity in mice reconstituted with human hematopoietic stem cells encoding defective FOXP3. Blood (2015) 1.48
The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo. Mol Ther Nucleic Acids (2014) 1.46
A simplified and efficient germline-specific CRISPR/Cas9 system for Drosophila genomic engineering. Fly (Austin) (2013) 1.44
Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol (2015) 1.43
Effective gene targeting in rabbits using RNA-guided Cas9 nucleases. J Mol Cell Biol (2014) 1.41
Genome editing technologies: defining a path to clinic. Mol Ther (2015) 1.41
Repurposing CRISPR/Cas9 for in situ functional assays. Genes Dev (2013) 1.41
A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol (2014) 1.37
Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system. Development (2014) 1.37
Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila. Cell Rep (2014) 1.35
Applications of CRISPR technologies in research and beyond. Nat Biotechnol (2016) 1.34
Protein neddylation: beyond cullin-RING ligases. Nat Rev Mol Cell Biol (2015) 1.33
Survivin as a preferential target for cancer therapy. Int J Mol Sci (2014) 1.28
COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites. Mol Ther Nucleic Acids (2014) 1.27
A framework for studying emotions across species. Cell (2014) 1.27
International regulatory landscape and integration of corrective genome editing into in vitro fertilization. Reprod Biol Endocrinol (2014) 1.27
Targeted genome editing of sweet orange using Cas9/sgRNA. PLoS One (2014) 1.26
Deciphering the functions and regulation of brain-enriched A-to-I RNA editing. Nat Neurosci (2013) 1.25
miRNA dysregulation in cancer: towards a mechanistic understanding. Front Genet (2014) 1.25
Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering. PLoS One (2014) 1.24
Promoter RNA links transcriptional regulation of inflammatory pathway genes. Nucleic Acids Res (2013) 1.24
A PCR based protocol for detecting indel mutations induced by TALENs and CRISPR/Cas9 in zebrafish. PLoS One (2014) 1.23
Efficient CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana and inheritance of modified genes in the T2 and T3 generations. PLoS One (2014) 1.20
Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation. Nat Rev Mol Cell Biol (2015) 1.20
Homology-driven genome editing in hematopoietic stem and progenitor cells using ZFN mRNA and AAV6 donors. Nat Biotechnol (2015) 1.17
Sound strategies for hearing restoration. Science (2014) 1.16
Quantifying on- and off-target genome editing. Trends Biotechnol (2015) 1.15
HBS1L-MYB intergenic variants modulate fetal hemoglobin via long-range MYB enhancers. J Clin Invest (2014) 1.14
The molecular era of the mitochondrial calcium uniporter. Nat Rev Mol Cell Biol (2015) 1.14
Comparing zinc finger nucleases and transcription activator-like effector nucleases for gene targeting in Drosophila. G3 (Bethesda) (2013) 1.14
TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis. Proc Natl Acad Sci U S A (2015) 1.14
Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) (2015) 1.13
Advances in genome editing technology and its promising application in evolutionary and ecological studies. Gigascience (2014) 1.11
Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems. Sci Rep (2014) 1.10
Correction of dystrophin expression in cells from Duchenne muscular dystrophy patients through genomic excision of exon 51 by zinc finger nucleases. Mol Ther (2014) 1.09
Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR. Mol Cell (2015) 1.09
Targeted genome engineering techniques in Drosophila. Methods (2014) 1.09
CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Cell Stem Cell (2016) 1.08
Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Biol Open (2014) 1.08
Generation of improved humanized mouse models for human infectious diseases. J Immunol Methods (2014) 1.08
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes. Cell (2016) 1.08
Poly peak parser: Method and software for identification of unknown indels using sanger sequencing of polymerase chain reaction products. Dev Dyn (2014) 1.07
Newer gene editing technologies toward HIV gene therapy. Viruses (2013) 1.07
Targeted methylation of the epithelial cell adhesion molecule (EpCAM) promoter to silence its expression in ovarian cancer cells. PLoS One (2014) 1.07
Chemical biology strategies for posttranslational control of protein function. Chem Biol (2014) 1.07
Long noncoding RNAs in cancer: from function to translation. Trends Cancer (2015) 1.06
Synthetic zinc finger proteins: the advent of targeted gene regulation and genome modification technologies. Acc Chem Res (2014) 1.06
Epigenetics and the regulation of stress vulnerability and resilience. Neuroscience (2013) 1.06
Mitochondrial Rab GAPs govern autophagosome biogenesis during mitophagy. Elife (2014) 1.06
Development of synthetic selfish elements based on modular nucleases in Drosophila melanogaster. Nucleic Acids Res (2014) 1.05
The innate immune function of airway epithelial cells in inflammatory lung disease. Eur Respir J (2015) 1.05
Gene therapies that restore dystrophin expression for the treatment of Duchenne muscular dystrophy. Hum Genet (2016) 1.04
Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template. Protein Cell (2014) 1.04
Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Sci Rep (2015) 1.03
Adenovirus-mediated efficient gene transfer into cultured three-dimensional organoids. PLoS One (2014) 1.03
Utilization of the AAVS1 safe harbor locus for hematopoietic specific transgene expression and gene knockdown in human ES cells. Stem Cell Res (2014) 1.02
TALEN knockout of the PSIP1 gene in human cells: analyses of HIV-1 replication and allosteric integrase inhibitor mechanism. J Virol (2014) 1.02
An integrated encyclopedia of DNA elements in the human genome. Nature (2012) 64.73
Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53
RNA-guided human genome engineering via Cas9. Science (2013) 48.29
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
A TALE nuclease architecture for efficient genome editing. Nat Biotechnol (2010) 26.47
Breaking the code of DNA binding specificity of TAL-type III effectors. Science (2009) 22.33
Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol (2013) 21.32
A simple cipher governs DNA recognition by TAL effectors. Science (2009) 19.99
Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature (2005) 19.15
Genome editing with engineered zinc finger nucleases. Nat Rev Genet (2010) 18.88
An improved zinc-finger nuclease architecture for highly specific genome editing. Nat Biotechnol (2007) 17.80
RNA-guided genetic silencing systems in bacteria and archaea. Nature (2012) 17.12
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res (2011) 16.53
RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol (2013) 15.51
RNA-programmed genome editing in human cells. Elife (2013) 14.82
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat Biotechnol (2008) 14.75
Targeting DNA double-strand breaks with TAL effector nucleases. Genetics (2010) 14.49
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat Biotechnol (2013) 14.28
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat Biotechnol (2008) 14.20
FLASH assembly of TALENs for high-throughput genome editing. Nat Biotechnol (2012) 12.97
Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol (2011) 12.86
Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol (2009) 12.40
Knockout rats via embryo microinjection of zinc-finger nucleases. Science (2009) 12.33
Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol (2011) 11.76
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell (2008) 11.24
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol (2008) 10.29
Targeted genome editing across species using ZFNs and TALENs. Science (2011) 9.84
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery. Nat Biotechnol (2007) 9.23
The long-range interaction landscape of gene promoters. Nature (2012) 9.20
Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics (2002) 9.09
Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Cell Stem Cell (2009) 8.97
Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases. Nat Biotechnol (2007) 8.88
Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA). Nat Methods (2010) 8.86
Knockout rats generated by embryo microinjection of TALENs. Nat Biotechnol (2011) 8.45
Enhancing gene targeting with designed zinc finger nucleases. Science (2003) 8.42
A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity. Nucleic Acids Res (2011) 7.93
Gene silencing in mammals by small interfering RNAs. Nat Rev Genet (2002) 7.80
High-frequency modification of plant genes using engineered zinc-finger nucleases. Nature (2009) 7.19
In vivo genome editing using a high-efficiency TALEN system. Nature (2012) 7.11
Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol (2011) 7.05
Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A (2008) 6.59
Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations. Cell (2011) 6.56
Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo. Nat Biotechnol (2010) 6.55
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Modular system for the construction of zinc-finger libraries and proteins. Nat Protoc (2010) 1.87
Rapid, stable, chemoselective labeling of thiols with Julia-Kocieński-like reagents: a serum-stable alternative to maleimide-based protein conjugation. Angew Chem Int Ed Engl (2013) 1.85
Chemically programmed monoclonal antibodies for cancer therapy: adaptor immunotherapy based on a covalent antibody catalyst. Proc Natl Acad Sci U S A (2003) 1.78
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Reading frame correction by targeted genome editing restores dystrophin expression in cells from Duchenne muscular dystrophy patients. Mol Ther (2013) 1.63
Biochemical characterization and structural analysis of a highly proficient cocaine esterase. Biochemistry (2002) 1.60
How vinculin regulates force transmission. Proc Natl Acad Sci U S A (2013) 1.51
Chimeric TALE recombinases with programmable DNA sequence specificity. Nucleic Acids Res (2012) 1.38
Small molecule drug activity in melanoma models may be dramatically enhanced with an antibody effector. Int J Cancer (2006) 1.37
Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors. Proc Natl Acad Sci U S A (2002) 1.34
In vivo site-specific DNA methylation with a designed sequence-enabled DNA methylase. J Am Chem Soc (2007) 1.33
Structure-guided reprogramming of serine recombinase DNA sequence specificity. Proc Natl Acad Sci U S A (2010) 1.31
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Exploring strategies for the design of artificial transcription factors: targeting sites proximal to known regulatory regions for the induction of gamma-globin expression and the treatment of sickle cell disease. J Biol Chem (2004) 1.30
Rabbit immune repertoires as sources for therapeutic monoclonal antibodies: the impact of kappa allotype-correlated variation in cysteine content on antibody libraries selected by phage display. J Mol Biol (2003) 1.30
Chemical adaptor immunotherapy: design, synthesis, and evaluation of novel integrin-targeting devices. J Med Chem (2004) 1.29
Evolution of programmable zinc finger-recombinases with activity in human cells. J Mol Biol (2007) 1.28
Breaking the one antibody-one target axiom. Proc Natl Acad Sci U S A (2006) 1.26
Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases. Nucleic Acids Res (2011) 1.25
Direct asymmetric alpha-fluorination of aldehydes. Angew Chem Int Ed Engl (2005) 1.25
Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries. Biomaterials (2004) 1.25
Instant immunity through chemically programmable vaccination and covalent self-assembly. Proc Natl Acad Sci U S A (2009) 1.25
Attenuation of HIV-1 replication in primary human cells with a designed zinc finger transcription factor. J Biol Chem (2004) 1.24
Chemically programmed antibodies: endothelin receptor targeting CovX-Bodies. Bioorg Med Chem Lett (2006) 1.22
Fusion proteins consisting of human immunodeficiency virus type 1 integrase and the designed polydactyl zinc finger protein E2C direct integration of viral DNA into specific sites. J Virol (2004) 1.20
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Highly active zinc-finger nucleases by extended modular assembly. Genome Res (2012) 1.17
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Targeted plasmid integration into the human genome by an engineered zinc-finger recombinase. Nucleic Acids Res (2011) 1.13
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Integrin alpha(v)beta3 targeted therapy for Kaposi's sarcoma with an in vitro evolved antibody. FASEB J (2002) 1.11
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Virus-based gene therapy strategies for bone regeneration. Biomaterials (2006) 1.01
Development of a small peptide tag for covalent labeling of proteins. Bioconjug Chem (2007) 1.00
Synthesis of beta-hydroxyaldehydes with stereogenic quaternary carbon centers by direct organocatalytic asymmetric aldol reactions. Angew Chem Int Ed Engl (2004) 1.00
Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from an immune b9 allotype rabbit antibody library. J Immunol Methods (2004) 1.00
Design and use of fluorogenic aldehydes for monitoring the progress of aldehyde transformations. J Am Chem Soc (2004) 0.99
Promoter-targeted phage display selections with preassembled synthetic zinc finger libraries for endogenous gene regulation. J Mol Biol (2004) 0.98
Direct asymmetric anti-Mannich-type reactions catalyzed by a designed amino acid. J Am Chem Soc (2006) 0.98
Thiourea-catalyzed highly enantio- and diastereoselective additions of oxindoles to nitroolefins: application to the formal synthesis of (+)-physostigmine. J Am Chem Soc (2009) 0.97
Controlling gene expression in plants using synthetic zinc finger transcription factors. Plant J (2002) 0.97
Highly enantio- and diastereoselective Mannich reactions of glycine Schiff bases with in situ generated N-Boc-imines catalyzed by a cinchona alkaloid thiourea. Org Lett (2010) 0.96
Proline-catalyzed one-step asymmetric synthesis of 5-hydroxy-(2E)-hexenal from acetaldehyde. J Org Chem (2002) 0.96
Regulation of transgene expression in plants with polydactyl zinc finger transcription factors. Proc Natl Acad Sci U S A (2002) 0.96
Expanding the concept of chemically programmable antibodies to RNA aptamers: chemically programmed biotherapeutics. Angew Chem Int Ed Engl (2010) 0.95
Construction of bispirooxindoles containing three quaternary stereocentres in a cascade using a single multifunctional organocatalyst. Nat Chem (2011) 0.95
Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody. Cancer Res (2005) 0.95
Organocatalytic direct asymmetric aldol reactions in water. J Am Chem Soc (2006) 0.95
Regulation of endogenous human gene expression by ligand-inducible TALE transcription factors. ACS Synth Biol (2013) 0.94
Catalysis of 3-pyrrolidinecarboxylic acid and related pyrrolidine derivatives in enantioselective anti-Mannich-type reactions: importance of the 3-acid group on pyrrolidine for stereocontrol. J Am Chem Soc (2007) 0.94
Small-molecule switches for zinc finger transcription factors. J Am Chem Soc (2003) 0.94
Direct observation of an enamine intermediate in amine catalysis. J Am Chem Soc (2009) 0.94
Monoclonal antibody therapeutics with up to five specificities: functional enhancement through fusion of target-specific peptides. MAbs (2013) 0.93
Improving the serum stability of site-specific antibody conjugates with sulfone linkers. Bioconjug Chem (2014) 0.93
Direct asymmetric organocatalytic Michael reactions of alpha,alpha-disubstituted aldehydes with beta-nitrostyrenes for the synthesis of quaternary carbon-containing products. Org Lett (2004) 0.92
Isolation of human prostate cancer cell reactive antibodies using phage display technology. J Immunol Methods (2004) 0.92
Asymmetric synthesis of quaternary alpha- and beta-amino acids and beta-lactams via proline-catalyzed mannich reactions with branched aldehyde donors. Org Lett (2004) 0.92