Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcoding.

PubWeight™: 3.81‹?› | Rank: Top 1%

🔗 View Article (PMC 3196379)

Published in Nat Biotechnol on October 02, 2011

Authors

Rong Lu1, Norma F Neff, Stephen R Quake, Irving L Weissman

Author Affiliations

1: School of Medicine, Stanford University, Stanford, CA, USA. rlu@stanford.edu

Articles citing this

Intravital imaging. Cell (2011) 3.18

Unravelling cancer stem cell potential. Nat Rev Cancer (2013) 3.06

Tissue-specific stem cells: lessons from the skeletal muscle satellite cell. Cell Stem Cell (2012) 2.68

Early specification of CD8+ T lymphocyte fates during adaptive immunity revealed by single-cell gene-expression analyses. Nat Immunol (2014) 2.52

Diverse and heritable lineage imprinting of early haematopoietic progenitors. Nature (2013) 2.19

Fundamental properties of unperturbed haematopoiesis from stem cells in vivo. Nature (2015) 2.06

Unravelling stem cell dynamics by lineage tracing. Nat Rev Mol Cell Biol (2013) 1.53

Quantitative evolutionary dynamics using high-resolution lineage tracking. Nature (2015) 1.41

Counting stem cells: methodological constraints. Nat Methods (2012) 1.35

Retroviral integrations in gene therapy trials. Mol Ther (2012) 1.34

Hematopoietic stem cells: concepts, definitions, and the new reality. Blood (2015) 1.30

Identification of a clonally expanding haematopoietic compartment in bone marrow. EMBO J (2012) 1.19

Clonal tracking of rhesus macaque hematopoiesis highlights a distinct lineage origin for natural killer cells. Cell Stem Cell (2014) 1.15

Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells. Proc Natl Acad Sci U S A (2012) 1.15

Analysis of the clonal growth and differentiation dynamics of primitive barcoded human cord blood cells in NSG mice. Blood (2013) 1.13

Hematopoietic stem cell engineering at a crossroads. Blood (2011) 1.10

Whole-organism lineage tracing by combinatorial and cumulative genome editing. Science (2016) 1.06

Drawing a high-resolution functional map of adeno-associated virus capsid by massively parallel sequencing. Nat Commun (2014) 1.04

Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy. Blood (2012) 1.04

Dynamics of HSPC repopulation in nonhuman primates revealed by a decade-long clonal-tracking study. Cell Stem Cell (2014) 1.02

Single-cell technologies are revolutionizing the approach to rare cells. Immunol Cell Biol (2015) 1.01

Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche. Nature (2016) 0.99

High efficiency restriction enzyme-free linear amplification-mediated polymerase chain reaction approach for tracking lentiviral integration sites does not abrogate retrieval bias. Hum Gene Ther (2012) 0.98

An in vivo functional screen uncovers miR-150-mediated regulation of hematopoietic injury response. Cell Rep (2012) 0.97

Multiplexing clonality: combining RGB marking and genetic barcoding. Nucleic Acids Res (2014) 0.96

Evaluating a ligation-mediated PCR and pyrosequencing method for the detection of clonal contribution in polyclonal retrovirally transduced samples. Hum Gene Ther Methods (2013) 0.95

The microRNA miR-148a functions as a critical regulator of B cell tolerance and autoimmunity. Nat Immunol (2016) 0.93

Lentiviral and targeted cellular barcoding reveals ongoing clonal dynamics of cell lines in vitro and in vivo. Genome Biol (2014) 0.92

Neural progenitor cells labeling with microbubble contrast agent for ultrasound imaging in vivo. Biomaterials (2013) 0.91

In vivo generation of DNA sequence diversity for cellular barcoding. Nucleic Acids Res (2014) 0.90

Impact of next-generation sequencing error on analysis of barcoded plasmid libraries of known complexity and sequence. Nucleic Acids Res (2014) 0.90

Checkpoints that control B cell development. J Clin Invest (2015) 0.89

Quantitative shearing linear amplification polymerase chain reaction: an improved method for quantifying lentiviral vector insertion sites in transplanted hematopoietic cell systems. Hum Gene Ther Methods (2015) 0.85

The niche in single-cell technologies. Immunol Cell Biol (2015) 0.85

Prostaglandin E2 enhances long-term repopulation but does not permanently alter inherent stem cell competitiveness. Blood (2013) 0.84

Hematopoietic stem/progenitor cell commitment to the megakaryocyte lineage. Blood (2016) 0.84

Single cells get together: High-resolution approaches to study the dynamics of early mouse development. Semin Cell Dev Biol (2015) 0.84

Building a lineage from single cells: genetic techniques for cell lineage tracking. Nat Rev Genet (2017) 0.83

An animated landscape representation of CD4+ T-cell differentiation, variability, and plasticity: insights into the behavior of populations versus cells. Eur J Immunol (2014) 0.83

Molecular neuroanatomy: a generation of progress. Trends Neurosci (2013) 0.83

Optical Barcoding for Single-Clone Tracking to Study Tumor Heterogeneity. Mol Ther (2017) 0.83

Hematopoietic stem cell expansion and generation: the ways to make a breakthrough. Blood Res (2015) 0.82

Gene expression of single human mesenchymal stem cell in response to fluid shear. J Tissue Eng (2012) 0.81

"Deep" sequencing accuracy and reproducibility using Roche/454 technology for inferring co-receptor usage in HIV-1. PLoS One (2014) 0.81

Computational promoter modeling identifies the modes of transcriptional regulation in hematopoietic stem cells. PLoS One (2014) 0.81

Transcription factor-mediated reprogramming toward hematopoietic stem cells. EMBO J (2015) 0.81

Clonal fate mapping quantifies the number of haematopoietic stem cells that arise during development. Nat Cell Biol (2016) 0.80

Genetic barcoding with fluorescent proteins for multiplexed applications. J Vis Exp (2015) 0.80

Asymmetric Cell Division in T Lymphocyte Fate Diversification. Trends Immunol (2015) 0.80

Development of a diverse human T-cell repertoire despite stringent restriction of hematopoietic clonality in the thymus. Proc Natl Acad Sci U S A (2015) 0.79

Long-term evolution is surprisingly predictable in lattice proteins. J R Soc Interface (2013) 0.78

Rapidly evolving homing CRISPR barcodes. Nat Methods (2016) 0.78

Upregulation of CD11A on hematopoietic stem cells denotes the loss of long-term reconstitution potential. Stem Cell Reports (2014) 0.78

You can count on this: barcoded hematopoietic stem cells. Cell Stem Cell (2011) 0.78

Comparison of transduction efficiency among various lentiviruses containing GFP reporter in bone marrow hematopoietic stem cell transplantation. Exp Hematol (2013) 0.78

Limitations and challenges of genetic barcode quantification. Sci Rep (2017) 0.78

Bone Density Loss Is Associated With Blood Cell Counts. J Bone Miner Res (2016) 0.77

Droplet microfluidics for kinetic studies of viral fusion. Biomicrofluidics (2016) 0.77

Mechanisms of blood homeostasis: lineage tracking and a neutral model of cell populations in rhesus macaques. BMC Biol (2015) 0.77

Reproducibility of Illumina platform deep sequencing errors allows accurate determination of DNA barcodes in cells. BMC Bioinformatics (2016) 0.77

Technology advancement for integrative stem cell analyses. Tissue Eng Part B Rev (2014) 0.77

Epigenetic Control of Haematopoietic Stem Cell Aging and Its Clinical Implications. Stem Cells Int (2015) 0.77

An exactly solvable model of random site-specific recombinations. Bull Math Biol (2012) 0.76

Theory and Practice of Lineage Tracing. Stem Cells (2015) 0.76

Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets. Sci Rep (2014) 0.76

Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells. Cell (2017) 0.76

Transgene expression in various organs post BM-HSC transplantation. Stem Cell Res (2013) 0.76

Digitally Barcoding Mycobacterium tuberculosis Reveals In Vivo Infection Dynamics in the Macaque Model of Tuberculosis. MBio (2017) 0.75

Gene Expression Correlates with the Number of Herpes Viral Genomes Initiating Infection in Single Cells. PLoS Pathog (2016) 0.75

Evidence for the in vivo safety of insulated foamy viral vectors. Gene Ther (2016) 0.75

What do we know about the participation of hematopoietic stem cells in hematopoiesis? F1000Res (2015) 0.75

The hematopoietic stem-cell niche in health and leukemia. Cell Mol Life Sci (2016) 0.75

A novel process of viral vector barcoding and library preparation enables high-diversity library generation and recombination-free paired-end sequencing. Sci Rep (2016) 0.75

Transplantation Dose Alters the Differentiation Program of Hematopoietic Stem Cells. Cell Rep (2016) 0.75

Clonal reversal of ageing-associated stem cell lineage bias via a pluripotent intermediate. Nat Commun (2017) 0.75

A generic, cost-effective, and scalable cell lineage analysis platform. Genome Res (2016) 0.75

The Clonal Fate of Live Cells. Mol Ther Methods Clin Dev (2017) 0.75

An in vivo multiplexed small-molecule screening platform. Nat Methods (2016) 0.75

Early commitment and robust differentiation in colonic crypts. Mol Syst Biol (2017) 0.75

Genetic Tagging During Human Mesoderm Differentiation Reveals Tripotent Lateral Plate Mesodermal Progenitors. Stem Cells (2016) 0.75

Quantitative Stability of Hematopoietic Stem and Progenitor Cell Clonal Output in Transplanted Rhesus Macaques. Blood (2017) 0.75

Lentiviral Fluorescent Genetic Barcoding for Multiplex Fate Tracking of Leukemic Cells. Mol Ther Methods Clin Dev (2017) 0.75

Cancer cells exhibit clonal diversity in phenotypic plasticity. Open Biol (2017) 0.75

Haematopoietic stem cells show their true colours. Nat Cell Biol (2016) 0.75

Unperturbed vs. post-transplantation hematopoiesis: both in vivo but different. Curr Opin Hematol (2016) 0.75

One stem cell at a time. Nat Methods (2011) 0.75

Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique. Open Biol (2017) 0.75

Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors. Nat Commun (2016) 0.75

Stem and progenitor cell subsets are affected by JAK2 signaling and can be monitored by flow cytometry. PLoS One (2014) 0.75

Transient loading of CD34(+) hematopoietic progenitor cells with polystyrene nanoparticles. Int J Nanomedicine (2017) 0.75

Genetic barcode sequencing for screening altered population dynamics of hematopoietic stem cells transduced with lentivirus. Mol Ther Methods Clin Dev (2014) 0.75

Site-specific recombinatorics: in situ cellular barcoding with the Cre Lox system. BMC Syst Biol (2016) 0.75

Inferring average generation via division-linked labeling. J Math Biol (2016) 0.75

Articles cited by this

Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci U S A (2003) 59.17

Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Nature (2006) 27.91

Strong association of de novo copy number mutations with autism. Science (2007) 27.84

SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell (2005) 24.00

A cell initiating human acute myeloid leukaemia after transplantation into SCID mice. Nature (1994) 21.59

A clonogenic common myeloid progenitor that gives rise to all myeloid lineages. Nature (2000) 16.75

Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell. Science (1996) 12.54

Association of reactive oxygen species levels and radioresistance in cancer stem cells. Nature (2009) 10.31

Cancer stem cells. N Engl J Med (2006) 9.63

A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature (2004) 8.67

Flk-2 is a marker in hematopoietic stem cell differentiation: a simple method to isolate long-term stem cells. Proc Natl Acad Sci U S A (2001) 7.58

Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors. Nature (1985) 7.54

Identification of genetic variants using bar-coded multiplexed sequencing. Nat Methods (2008) 7.50

Stem cells: units of development, units of regeneration, and units in evolution. Cell (2000) 7.36

Introduction of a selectable gene into primitive stem cells capable of long-term reconstitution of the hemopoietic system of W/Wv mice. Cell (1985) 6.88

Stem cell concepts renew cancer research. Blood (2008) 6.74

Stems cells and the pathways to aging and cancer. Cell (2008) 5.99

Developmental potential and dynamic behavior of hematopoietic stem cells. Cell (1986) 5.98

Hematopoietic stem cell gene transfer in a tumor-prone mouse model uncovers low genotoxicity of lentiviral vector integration. Nat Biotechnol (2006) 5.58

The increasing complexity of the cancer stem cell paradigm. Science (2009) 5.45

Long-term propagation of distinct hematopoietic differentiation programs in vivo. Cell Stem Cell (2007) 5.14

Hematopoietic stem cells: the paradigmatic tissue-specific stem cell. Am J Pathol (2006) 4.32

Clonal and systemic analysis of long-term hematopoiesis in the mouse. Genes Dev (1990) 4.17

Epithelial stem cells: turning over new leaves. Cell (2007) 4.10

High-resolution insertion-site analysis by linear amplification-mediated PCR (LAM-PCR). Nat Methods (2007) 3.90

Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking. Science (2005) 3.58

Functionally distinct hematopoietic stem cells modulate hematopoietic lineage potential during aging by a mechanism of clonal expansion. Proc Natl Acad Sci U S A (2010) 3.41

High-throughput SELEX SAGE method for quantitative modeling of transcription-factor binding sites. Nat Biotechnol (2002) 3.04

Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment. J Exp Med (2010) 2.98

The hematopoietic stem compartment consists of a limited number of discrete stem cell subsets. Blood (2005) 2.79

Individual stem cells with highly variable proliferation and self-renewal properties comprise the human hematopoietic stem cell compartment. Nat Immunol (2006) 2.60

Hematopoietic stem cell: self-renewal versus differentiation. Wiley Interdiscip Rev Syst Biol Med (2010) 2.38

CD150- side population cells represent a functionally distinct population of long-term hematopoietic stem cells. Blood (2007) 2.27

A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment but not individual stem cells. Blood (2008) 2.23

Sensitivity of noninvasive prenatal detection of fetal aneuploidy from maternal plasma using shotgun sequencing is limited only by counting statistics. PLoS One (2010) 2.11

Flk2+ common lymphoid progenitors possess equivalent differentiation potential for the B and T lineages. Blood (2008) 2.06

Looking ahead in cancer stem cell research. Nat Biotechnol (2009) 1.89

Cellular barcoding tool for clonal analysis in the hematopoietic system. Blood (2010) 1.81

Recruitment of antigen-specific CD8+ T cells in response to infection is markedly efficient. Science (2009) 1.70

Lentivector-mediated clonal tracking reveals intrinsic heterogeneity in the human hematopoietic stem cell compartment and culture-induced stem cell impairment. Blood (2003) 1.60

Tracking adult stem cells. EMBO Rep (2011) 1.56

Lnk negatively regulates self-renewal of hematopoietic stem cells by modifying thrombopoietin-mediated signal transduction. Proc Natl Acad Sci U S A (2007) 1.55

Dissecting T cell lineage relationships by cellular barcoding. J Exp Med (2008) 1.53

Multiarm high-throughput integration site detection: limitations of LAM-PCR technology and optimization for clonal analysis. Stem Cells Dev (2007) 1.32

Unaltered repopulation properties of mouse hematopoietic stem cells transduced with lentiviral vectors. Blood (2008) 1.02

Local clonal analysis of the hematopoietic system shows that multiple small short-living clones maintain life-long hematopoiesis in reconstituted mice. Blood (1996) 1.00

Polyclonal fluctuation of lentiviral vector-transduced and expanded murine hematopoietic stem cells. Blood (2011) 0.97

High-throughput, sensitive quantification of repopulating hematopoietic stem cell clones. J Virol (2010) 0.95

Contributions of gene marking to cell and gene therapies. Hum Gene Ther (2011) 0.95

Low-dose total body irradiation causes clonal fluctuation of primate hematopoietic stem and progenitor cells. Blood (2004) 0.95

Articles by these authors

Cancer stem cells--perspectives on current status and future directions: AACR Workshop on cancer stem cells. Cancer Res (2006) 17.42

Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells. Nature (2003) 14.35

A role for Wnt signalling in self-renewal of haematopoietic stem cells. Nature (2003) 12.81

Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature (2003) 12.54

Single-molecule DNA sequencing of a viral genome. Science (2008) 11.66

Granulocyte-macrophage progenitors as candidate leukemic stem cells in blast-crisis CML. N Engl J Med (2004) 11.29

Rejuvenation of aged progenitor cells by exposure to a young systemic environment. Nature (2005) 11.23

Association of reactive oxygen species levels and radioresistance in cancer stem cells. Nature (2009) 10.31

Clinical assessment incorporating a personal genome. Lancet (2010) 10.18

Little evidence for developmental plasticity of adult hematopoietic stem cells. Science (2002) 9.63

Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood. Proc Natl Acad Sci U S A (2008) 8.38

Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium. Nature (2004) 8.36

Single-molecule sequencing of an individual human genome. Nat Biotechnol (2009) 8.35

Single-cell dissection of transcriptional heterogeneity in human colon tumors. Nat Biotechnol (2011) 7.84

Dissecting biological "dark matter" with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth. Proc Natl Acad Sci U S A (2007) 7.82

Biology of hematopoietic stem cells and progenitors: implications for clinical application. Annu Rev Immunol (2002) 7.78

Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Nature (2007) 7.72

Microfluidic large-scale integration. Science (2002) 7.64

Langerhans cells renew in the skin throughout life under steady-state conditions. Nat Immunol (2002) 7.03

Whole-genome molecular haplotyping of single cells. Nat Biotechnol (2010) 6.77

Cell intrinsic alterations underlie hematopoietic stem cell aging. Proc Natl Acad Sci U S A (2005) 6.32

Comprehensive methylome map of lineage commitment from haematopoietic progenitors. Nature (2010) 6.06

Stems cells and the pathways to aging and cancer. Cell (2008) 5.99

Developing optofluidic technology through the fusion of microfluidics and optics. Nature (2006) 5.82

Global analysis of proliferation and cell cycle gene expression in the regulation of hematopoietic stem and progenitor cell fates. J Exp Med (2005) 5.68

CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells. Cell (2009) 5.30

Elucidation of the phenotypic, functional, and molecular topography of a myeloerythroid progenitor cell hierarchy. Cell Stem Cell (2007) 5.28

Single-cell NF-kappaB dynamics reveal digital activation and analogue information processing. Nature (2010) 5.27

Sequence information can be obtained from single DNA molecules. Proc Natl Acad Sci U S A (2003) 5.24

CD47 is upregulated on circulating hematopoietic stem cells and leukemia cells to avoid phagocytosis. Cell (2009) 5.22

Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR. Proc Natl Acad Sci U S A (2006) 5.21

Isolation of adult mouse myogenic progenitors: functional heterogeneity of cells within and engrafting skeletal muscle. Cell (2004) 5.20

Plasticity of adult stem cells. Cell (2004) 5.13

A systems approach to measuring the binding energy landscapes of transcription factors. Science (2007) 5.06

Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271. Nature (2010) 5.05

Similar MLL-associated leukemias arising from self-renewing stem cells and short-lived myeloid progenitors. Genes Dev (2003) 4.71

In vivo visualization of embryonic stem cell survival, proliferation, and migration after cardiac delivery. Circulation (2006) 4.48

High-throughput sequencing of the zebrafish antibody repertoire. Science (2009) 4.46

Shifting foci of hematopoiesis during reconstitution from single stem cells. Proc Natl Acad Sci U S A (2003) 4.41

Myeloid or lymphoid promiscuity as a critical step in hematopoietic lineage commitment. Dev Cell (2002) 4.37

Hematopoietic stem cells: the paradigmatic tissue-specific stem cell. Am J Pathol (2006) 4.32

JunB deficiency leads to a myeloproliferative disorder arising from hematopoietic stem cells. Cell (2004) 4.28

The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors. Proc Natl Acad Sci U S A (2012) 4.20

A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion. Proc Natl Acad Sci U S A (2002) 4.16

Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver. Genome Res (2009) 4.11

Normal and leukemic hematopoiesis: are leukemias a stem cell disorder or a reacquisition of stem cell characteristics? Proc Natl Acad Sci U S A (2003) 4.09

Clonal evolution of preleukemic hematopoietic stem cells precedes human acute myeloid leukemia. Sci Transl Med (2012) 4.05

Genome of a low-salinity ammonia-oxidizing archaeon determined by single-cell and metagenomic analysis. PLoS One (2011) 4.04

Coronary arteries form by developmental reprogramming of venous cells. Nature (2010) 3.95

Cancer stem cells in solid tumors. Curr Opin Biotechnol (2007) 3.91

Endochondral ossification is required for haematopoietic stem-cell niche formation. Nature (2008) 3.87

Identification, molecular characterization, clinical prognosis, and therapeutic targeting of human bladder tumor-initiating cells. Proc Natl Acad Sci U S A (2009) 3.87

Microfluidic digital PCR enables multigene analysis of individual environmental bacteria. Science (2006) 3.84

Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. Nat Med (2009) 3.82

Flt3 ligand regulates dendritic cell development from Flt3+ lymphoid and myeloid-committed progenitors to Flt3+ dendritic cells in vivo. J Exp Med (2003) 3.74

Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines. PLoS One (2012) 3.74

Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood. Cell Stem Cell (2007) 3.59

Long-term monitoring of bacteria undergoing programmed population control in a microchemostat. Science (2005) 3.54

Genome-wide single-cell analysis of recombination activity and de novo mutation rates in human sperm. Cell (2012) 3.52

Prospective isolation of human clonogenic common myeloid progenitors. Proc Natl Acad Sci U S A (2002) 3.50

An integrated microfabricated cell sorter. Anal Chem (2002) 3.43

Functionally distinct hematopoietic stem cells modulate hematopoietic lineage potential during aging by a mechanism of clonal expansion. Proc Natl Acad Sci U S A (2010) 3.41

Preleukemic mutations in human acute myeloid leukemia affect epigenetic regulators and persist in remission. Proc Natl Acad Sci U S A (2014) 3.39

Anti-CD47 antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma. Cell (2010) 3.37

Microfluidic large-scale integration: the evolution of design rules for biological automation. Annu Rev Biophys Biomol Struct (2007) 3.32

Genomic determination of the glucocorticoid response reveals unexpected mechanisms of gene regulation. Genome Res (2009) 3.30

Bone marrow-derived circulating endothelial precursors do not contribute to vascular endothelium and are not needed for tumor growth. Proc Natl Acad Sci U S A (2008) 3.27

Engineered SIRPα variants as immunotherapeutic adjuvants to anticancer antibodies. Science (2013) 3.25

Versatile, fully automated, microfluidic cell culture system. Anal Chem (2007) 3.19

Depletion of host Langerhans cells before transplantation of donor alloreactive T cells prevents skin graft-versus-host disease. Nat Med (2004) 3.17

Non-invasive prenatal measurement of the fetal genome. Nature (2012) 3.11

Differential expression of novel potential regulators in hematopoietic stem cells. PLoS Genet (2005) 3.11

An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells. Nat Biotechnol (2011) 3.05

Germ-layer and lineage-restricted stem/progenitors regenerate the mouse digit tip. Nature (2011) 3.03

Isolation and characterization of a protochordate histocompatibility locus. Nature (2005) 2.96

Human bone marrow hematopoietic stem cells are increased in frequency and myeloid-biased with age. Proc Natl Acad Sci U S A (2011) 2.96

Hematopoietic stem cells are uniquely selective in their migratory response to chemokines. J Exp Med (2002) 2.96

The JAK2 V617F mutation occurs in hematopoietic stem cells in polycythemia vera and predisposes toward erythroid differentiation. Proc Natl Acad Sci U S A (2006) 2.94

A synthetic Escherichia coli predator-prey ecosystem. Mol Syst Biol (2008) 2.92

Hematopoietic stem and progenitor cells: clinical and preclinical regeneration of the hematolymphoid system. Annu Rev Med (2005) 2.92

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nat Biotechnol (2011) 2.87

Transcriptional and functional profiling of human embryonic stem cell-derived cardiomyocytes. PLoS One (2008) 2.85

Multistep synthesis of a radiolabeled imaging probe using integrated microfluidics. Science (2005) 2.83

Memory T and memory B cells share a transcriptional program of self-renewal with long-term hematopoietic stem cells. Proc Natl Acad Sci U S A (2006) 2.83

Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47. Sci Transl Med (2010) 2.79

Nanoliter reactors improve multiple displacement amplification of genomes from single cells. PLoS Genet (2007) 2.79

Dysregulated gene expression networks in human acute myelogenous leukemia stem cells. Proc Natl Acad Sci U S A (2009) 2.76

Temporal response of the human virome to immunosuppression and antiviral therapy. Cell (2013) 2.72

Lineage structure of the human antibody repertoire in response to influenza vaccination. Sci Transl Med (2013) 2.71

Efficient transplantation via antibody-based clearance of hematopoietic stem cell niches. Science (2007) 2.69

Extranodal dissemination of non-Hodgkin lymphoma requires CD47 and is inhibited by anti-CD47 antibody therapy. Blood (2011) 2.69

microRNA-29a induces aberrant self-renewal capacity in hematopoietic progenitors, biased myeloid development, and acute myeloid leukemia. J Exp Med (2010) 2.68

Integrated nanoliter systems. Nat Biotechnol (2003) 2.68