Published in Nucleic Acids Res on August 11, 1988
Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res (1989) 19.85
Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci U S A (1990) 10.01
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Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev (1998) 8.51
Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor. EMBO J (1995) 7.60
A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res (1989) 6.78
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Ion channel activity of influenza A virus M2 protein: characterization of the amantadine block. J Virol (1993) 4.76
NS3 is a serine protease required for processing of hepatitis C virus polyprotein. J Virol (1993) 4.60
Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication. J Virol (1995) 4.27
Construction of mutant and chimeric genes using the polymerase chain reaction. Nucleic Acids Res (1989) 4.19
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Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1-carboxylate synthase in tomato. Proc Natl Acad Sci U S A (1990) 3.85
The large form of hepatitis delta antigen is crucial for assembly of hepatitis delta virus. Proc Natl Acad Sci U S A (1991) 3.52
Programmable bacteria detect and record an environmental signal in the mammalian gut. Proc Natl Acad Sci U S A (2014) 3.50
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products. Nucleic Acids Res (2007) 3.43
Second locus involved in human immunodeficiency virus type 1 resistance to protease inhibitors. J Virol (1996) 3.39
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Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence. J Cell Biol (1993) 3.30
The full-length leptin receptor has signaling capabilities of interleukin 6-type cytokine receptors. Proc Natl Acad Sci U S A (1996) 3.21
A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway. EMBO J (1998) 3.13
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR). Nucleic Acids Res (1989) 2.90
Essential functions and actin-binding surfaces of yeast cofilin revealed by systematic mutagenesis. EMBO J (1997) 2.88
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Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO J (1998) 2.82
Protein targeting by tyrosine- and di-leucine-based signals: evidence for distinct saturable components. J Cell Biol (1996) 2.78
Two independent targeting signals in the cytoplasmic domain determine trans-Golgi network localization and endosomal trafficking of the proprotein convertase furin. EMBO J (1995) 2.76
Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli. Proc Natl Acad Sci U S A (1991) 2.75
Sar1 promotes vesicle budding from the endoplasmic reticulum but not Golgi compartments. J Cell Biol (1994) 2.75
Conformational flexibility in the multidrug efflux system protein AcrA. Structure (2006) 2.75
Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3' UTR and does not involve poly(A) tail shortening. EMBO J (1994) 2.70
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Monoubiquitin carries a novel internalization signal that is appended to activated receptors. EMBO J (2000) 2.54
An acidic sequence within the cytoplasmic domain of furin functions as a determinant of trans-Golgi network localization and internalization from the cell surface. EMBO J (1995) 2.54
Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesicles. J Cell Biol (1993) 2.51
A general and fast method to generate multiple site directed mutations. Nucleic Acids Res (1992) 2.51
RGD sequence of foot-and-mouth disease virus is essential for infecting cells via the natural receptor but can be bypassed by an antibody-dependent enhancement pathway. Proc Natl Acad Sci U S A (1994) 2.49
A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J (1995) 2.47
Rescue of rinderpest virus from cloned cDNA. J Virol (1997) 2.44
Critical contacts between HIV-1 integrase and viral DNA identified by structure-based analysis and photo-crosslinking. EMBO J (1997) 2.42
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Early events in preprotein recognition in E. coli: interaction of SRP and trigger factor with nascent polypeptides. EMBO J (1995) 2.38
Spontaneous cleavage of RNA in ternary complexes of Escherichia coli RNA polymerase and its significance for the mechanism of transcription. Proc Natl Acad Sci U S A (1991) 2.36
A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase. Nucleic Acids Res (1994) 2.35
T-cell and basophil activation through the cytoplasmic tail of T-cell-receptor zeta family proteins. Proc Natl Acad Sci U S A (1991) 2.32
Mitochondria-to-nucleus stress signaling induces phenotypic changes, tumor progression and cell invasion. EMBO J (2001) 2.30
Mutagenesis of the putative fusion domain of the Semliki Forest virus spike protein. J Virol (1991) 2.30
A lumenal domain-dependent pathway for sorting to intralumenal vesicles of multivesicular endosomes involved in organelle morphogenesis. Dev Cell (2006) 2.29
The cytoplasmic domain mediates localization of furin to the trans-Golgi network en route to the endosomal/lysosomal system. J Cell Biol (1994) 2.25
USER friendly DNA engineering and cloning method by uracil excision. Nucleic Acids Res (2007) 2.24
Resistance to 2',3'-dideoxycytidine conferred by a mutation in codon 65 of the human immunodeficiency virus type 1 reverse transcriptase. Antimicrob Agents Chemother (1994) 2.24
The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascade. J Biol Chem (2007) 2.24
The tomato R gene products I-2 and MI-1 are functional ATP binding proteins with ATPase activity. Plant Cell (2002) 2.22
Rapid cloning by homologous recombination in vivo. Nucleic Acids Res (1993) 2.19
A potent transrepression domain in the retinoblastoma protein induces a cell cycle arrest when bound to E2F sites. Proc Natl Acad Sci U S A (1995) 2.17
Transcriptional regulator PRDM12 is essential for human pain perception. Nat Genet (2015) 2.15
RNA-RNA interaction is required for the formation of specific bicoid mRNA 3' UTR-STAUFEN ribonucleoprotein particles. EMBO J (1997) 2.13
Proprotein convertase cleavage liberates a fibrillogenic fragment of a resident glycoprotein to initiate melanosome biogenesis. J Cell Biol (2003) 2.12
Tomato SP-interacting proteins define a conserved signaling system that regulates shoot architecture and flowering. Plant Cell (2001) 2.11
Comprehensive mutant enzyme and viral variant assessment of human immunodeficiency virus type 1 reverse transcriptase resistance to nonnucleoside inhibitors. Antimicrob Agents Chemother (1993) 2.10
Catalytic domain of human immunodeficiency virus type 1 integrase: identification of a soluble mutant by systematic replacement of hydrophobic residues. Proc Natl Acad Sci U S A (1995) 2.09
Specific association of the gene product of PKD2 with the TRPC1 channel. Proc Natl Acad Sci U S A (1999) 2.09
Binding of Bruton's tyrosine kinase to Fyn, Lyn, or Hck through a Src homology 3 domain-mediated interaction. Proc Natl Acad Sci U S A (1994) 2.06
Rapid cell-surface prion protein conversion revealed using a novel cell system. Nat Commun (2011) 2.04
Csk inhibition of c-Src activity requires both the SH2 and SH3 domains of Src. EMBO J (1993) 2.02
Interactions with PIP2, ADP-actin monomers, and capping protein regulate the activity and localization of yeast twinfilin. J Cell Biol (2001) 1.97
Polyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis. J Virol (1996) 1.96
Beta-casein gene promoter activity is regulated by the hormone-mediated relief of transcriptional repression and a mammary-gland-specific nuclear factor. Mol Cell Biol (1991) 1.93
A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR. Nucleic Acids Res (1997) 1.93
Negative regulation of tumor suppressor p53 by microRNA miR-504. Mol Cell (2010) 1.92
The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipids. EMBO J (1993) 1.91
Structure-guided recombination creates an artificial family of cytochromes P450. PLoS Biol (2006) 1.90
Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein: implications for morphogenesis and organization of encapsidated RNA. Proc Natl Acad Sci U S A (1997) 1.90
Identification and characterization of a cis-acting replication element (cre) adjacent to the internal ribosome entry site of foot-and-mouth disease virus. J Virol (2002) 1.88
A novel membrane-associated metalloprotease, Ste24p, is required for the first step of NH2-terminal processing of the yeast a-factor precursor. J Cell Biol (1997) 1.87
Chromatin opening and transactivator potentiation by RAP1 in Saccharomyces cerevisiae. Mol Cell Biol (1999) 1.87
Identification of Lassa virus glycoprotein signal peptide as a trans-acting maturation factor. EMBO Rep (2003) 1.85
Role of tryptophan repeats and flanking amino acids in Myb-DNA interactions. Proc Natl Acad Sci U S A (1990) 1.84
Protein-protein interaction panel using mouse full-length cDNAs. Genome Res (2001) 1.84
Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: evolutionary implications. Proc Natl Acad Sci U S A (1992) 1.82
Site-specific mutagenesis using asymmetric polymerase chain reaction and a single mutant primer. Nucleic Acids Res (1990) 1.82
Multiple closely-linked NFAT/octamer and HMG I(Y) binding sites are part of the interleukin-4 promoter. Nucleic Acids Res (1993) 1.80
A lysosomal targeting signal in the cytoplasmic tail of the beta chain directs HLA-DM to MHC class II compartments. J Cell Biol (1995) 1.78
Specificity for the hairy/enhancer of split basic helix-loop-helix (bHLH) proteins maps outside the bHLH domain and suggests two separable modes of transcriptional repression. Mol Cell Biol (1995) 1.77
Site-directed mutagenesis reveals a liver transcription factor essential for the albumin transcriptional enhancer. Proc Natl Acad Sci U S A (1990) 1.77
Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59. J Virol (1995) 1.76
Mutations in the NB-ARC domain of I-2 that impair ATP hydrolysis cause autoactivation. Plant Physiol (2006) 1.75
N-terminal DNA-binding domains contribute to differential DNA-binding specificities of NF-kappa B p50 and p65. Mol Cell Biol (1993) 1.75
Identification of the catalytic sites of a papain-like cysteine proteinase of murine coronavirus. J Virol (1993) 1.74
Hepatitis B virus core antigen has two nuclear localization sequences in the arginine-rich carboxyl terminus. J Virol (1991) 1.73
Two nonallelic insulin genes in Xenopus laevis are expressed differentially during neurulation in prepancreatic embryos. Proc Natl Acad Sci U S A (1991) 1.72
Diversification of catalytic function in a synthetic family of chimeric cytochrome p450s. Chem Biol (2007) 1.71
Absence of a stable intermediate on the folding pathway of protein A. Protein Sci (1997) 1.71
Occurrence and characterization of mercury resistance in the hyperthermophilic archaeon Sulfolobus solfataricus by use of gene disruption. J Bacteriol (2004) 1.70
TRIF and IRF-3 binding to the TNF promoter results in macrophage TNF dysregulation and steatosis induced by chronic ethanol. J Immunol (2008) 1.70
Structures of receptor complexes of a North American H7N2 influenza hemagglutinin with a loop deletion in the receptor binding site. PLoS Pathog (2010) 1.69
Solution structures of cytosolic RNA sensor MDA5 and LGP2 C-terminal domains: identification of the RNA recognition loop in RIG-I-like receptors. J Biol Chem (2009) 1.68
Gab1 acts as an adapter molecule linking the cytokine receptor gp130 to ERK mitogen-activated protein kinase. Mol Cell Biol (1998) 1.66
Multistep phosphorelay proteins transmit oxidative stress signals to the fission yeast stress-activated protein kinase. Mol Biol Cell (2000) 1.66
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science (1988) 220.77
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science (1985) 95.08
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol (1987) 58.68
Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science (1986) 19.54
Purification and biochemical characterization of the promoter-specific transcription factor, Sp1. Science (1986) 12.58
Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA. Nucleic Acids Res (1987) 10.38
DNA typing from single hairs. Nature (1988) 9.32
The interaction of RNA polymerase and lac repressor with the lac control region. Nucleic Acids Res (1979) 6.17
A simple procedure for resolution of Escherichia coli RNA polymerase holoenzyme from core polymerase. Arch Biochem Biophys (1977) 4.85
Isolation and properties of transcribing ternary complexes of Escherichia coli RNA polymerase positioned at a single template base. J Mol Biol (1987) 4.83
RNA polymerase unwinds an 11-base pair segment of a phage T7 promoter. Nature (1979) 4.63
Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4. J Mol Biol (1981) 4.56
A T5 promoter-based transcription-translation system for the analysis of proteins in vitro and in vivo. Methods Enzymol (1987) 4.21
Intermediates in transcription initiation from the E. coli lac UV5 promoter. Cell (1985) 3.88
DNA amplification in vitro using T4 DNA polymerase. DNA (1988) 2.45
Rapid construction of large synthetic genes: total chemical synthesis of two different versions of the bovine prochymosin gene. Gene (1987) 2.36
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science (1985) 95.08
Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes. Nature (1986) 17.77
Amplification and analysis of DNA sequences in single human sperm and diploid cells. Nature (1988) 10.84
Characterization of beta-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA. Nature (1987) 8.58
Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J Biol Chem (1989) 4.96
High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. PCR Methods Appl (1993) 4.83
Isolation and properties of transcribing ternary complexes of Escherichia coli RNA polymerase positioned at a single template base. J Mol Biol (1987) 4.83
Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides. Biochem Biophys Res Commun (1987) 4.05
Rapid prenatal diagnosis of sickle cell anemia by a new method of DNA analysis. N Engl J Med (1987) 3.44
T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells. Proc Natl Acad Sci U S A (1980) 3.32
Analysis of RAS gene mutations in acute myeloid leukemia by polymerase chain reaction and oligonucleotide probes. Proc Natl Acad Sci U S A (1988) 2.99
Detection of ras gene mutations in pancreatic juice and peripheral blood of patients with pancreatic adenocarcinoma. Cancer Res (1993) 2.22
A mutation that prevents glucosylation of the lipid-linked oligosaccharide precursor leads to underglycosylation of secreted yeast invertase. Proc Natl Acad Sci U S A (1986) 1.15
Rapid prenatal diagnosis of beta thalassemia using DNA amplification and nonradioactive probes. Blood (1989) 0.92
Genetic diversity within the HLA class II region: ten new DPB1 alleles and their population distribution. Tissue Antigens (1992) 0.89
Analysis of 16 cystic fibrosis mutations in Mexican patients. Am J Med Genet (1997) 0.80
Prenatal diagnosis of alpha-thalassemia by polymerase chain reaction and dual restriction enzyme analysis. Hum Genet (1990) 0.79
T-DNA of a crown gall teratoma is covalently joined to host plant DNA. Nature (1980) 0.77
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. 1985. Biotechnology (1992) 0.76
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