Published in J Cell Biol on December 01, 1985
Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol (1988) 13.27
A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum. J Cell Biol (1987) 4.94
A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. J Cell Biol (1989) 4.29
Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Mol Biol Cell (1992) 3.81
Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily. Mol Cell Biol (1991) 3.68
A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast. J Cell Biol (1988) 3.37
The promoter region of the yeast KAR2 (BiP) gene contains a regulatory domain that responds to the presence of unfolded proteins in the endoplasmic reticulum. Mol Cell Biol (1993) 3.25
The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport. Mol Cell Biol (1991) 2.87
Yeast Sec23p acts in the cytoplasm to promote protein transport from the endoplasmic reticulum to the Golgi complex in vivo and in vitro. EMBO J (1989) 2.64
Genetic and biochemical characterization of clathrin-deficient Saccharomyces cerevisiae. Mol Cell Biol (1987) 2.50
Structural and functional dissection of Sec62p, a membrane-bound component of the yeast endoplasmic reticulum protein import machinery. Mol Cell Biol (1990) 2.36
Continued functioning of the secretory pathway is essential for ribosome synthesis. Mol Cell Biol (1994) 2.20
Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor. J Cell Biol (1986) 1.66
Mapping of residues forming the voltage sensor of the voltage-dependent anion-selective channel. Proc Natl Acad Sci U S A (1993) 1.52
Secretory vesicles externalize the major plasma membrane ATPase in yeast. J Cell Biol (1988) 1.37
Sec59 encodes a membrane protein required for core glycosylation in Saccharomyces cerevisiae. Mol Cell Biol (1989) 1.21
The FKB2 gene of Saccharomyces cerevisiae, encoding the immunosuppressant-binding protein FKBP-13, is regulated in response to accumulation of unfolded proteins in the endoplasmic reticulum. Proc Natl Acad Sci U S A (1993) 1.16
Sec53, a protein required for an early step in secretory protein processing and transport in yeast, interacts with the cytoplasmic surface of the endoplasmic reticulum. Proc Natl Acad Sci U S A (1987) 1.14
PMI40, an intron-containing gene required for early steps in yeast mannosylation. Mol Cell Biol (1992) 1.08
In vivo evidence for posttranslational translocation and signal cleavage of the killer preprotoxin of Saccharomyces cerevisiae. Mol Cell Biol (1986) 0.86
Expression of the RNA genome of an animal virus in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1986) 0.86
Using Gene Essentiality and Synthetic Lethality Information to Correct Yeast and CHO Cell Genome-Scale Models. Metabolites (2015) 0.77
Secretion in yeast: in vitro analysis of the sec53 mutant. EMBO J (1988) 0.77
Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (1970) 1528.65
DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A (1977) 790.54
A simple method for displaying the hydropathic character of a protein. J Mol Biol (1982) 150.65
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene (1982) 102.30
Transformation of intact yeast cells treated with alkali cations. J Bacteriol (1983) 100.26
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem (1981) 71.15
Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature (1962) 64.93
An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem (1976) 46.42
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol (1980) 41.18
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem (1978) 38.54
Yeast transformation: a model system for the study of recombination. Proc Natl Acad Sci U S A (1981) 26.54
Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase. Cell (1982) 25.10
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell (1980) 23.17
Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro. Proc Natl Acad Sci U S A (1977) 18.85
Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene (1979) 16.95
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo. Proc Natl Acad Sci U S A (1980) 16.88
A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol (1946) 16.78
DNA sequence required for efficient transcription termination in yeast. Cell (1982) 14.19
Easy identification of cDNA clones. EMBO J (1983) 9.18
Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc Natl Acad Sci U S A (1980) 7.81
Glycosylation and processing of prepro-alpha-factor through the yeast secretory pathway. Cell (1984) 5.53
Compartmentalized assembly of oligosaccharides on exported glycoproteins in yeast. Cell (1981) 5.51
Lyticase: endoglucanase and protease activities that act together in yeast cell lysis. J Bacteriol (1980) 4.25
Invertase signal and mature sequence substitutions that delay intercompartmental transport of active enzyme. J Cell Biol (1985) 4.24
Yeast secretory mutants that block the formation of active cell surface enzymes. J Cell Biol (1984) 2.71
Yeast mutants deficient in protein glycosylation. Proc Natl Acad Sci U S A (1983) 2.55
Selection of functional cDNAs by complementation in yeast. Proc Natl Acad Sci U S A (1983) 2.47
Import of proteins into mitochondria: a 70 kilodalton outer membrane protein with a large carboxy-terminal deletion is still transported to the outer membrane. EMBO J (1983) 2.33
Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K. Proc Natl Acad Sci U S A (1983) 2.14
Genes required for completion of import of proteins into the endoplasmic reticulum in yeast. J Cell Biol (1984) 2.00
Studies on the microsomal electron-transport system of anaerobically grown yeast. V. Purification and characterization of NADPH-cytochrome c reductase. J Biochem (1977) 1.96
Conformational changes associated with proteolytic processing of presecretory proteins allow glutathione-catalyzed formation of native disulfide bonds. J Biol Chem (1982) 1.72
Biosynthesis of the core region of yeast mannoproteins. Formation of a glucosylated dolichol-bound oligosaccharide precursor, its transfer to protein and subsequent modification. Eur J Biochem (1980) 1.36
Characterization of large oligosaccharide-lipids synthesized in vitro by microsomes from Saccharomyces cerevisiae. J Biol Chem (1980) 1.02
Regulation of expression of the ADE3 gene for yeast C1-tetrahydrofolate synthase, a trifunctional enzyme involved in one-carbon metabolism. J Biol Chem (1985) 0.90
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell (1980) 23.17
Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron (2000) 22.02
A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides. Nature (1988) 11.00
Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole. Cell (1982) 10.55
Order of events in the yeast secretory pathway. Cell (1981) 10.13
COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum. Cell (1994) 10.01
Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell (1990) 8.98
RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form. Proc Natl Acad Sci U S A (1972) 8.09
Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1979) 8.03
Nuclear localization of the C2H2 zinc finger protein Msn2p is regulated by stress and protein kinase A activity. Genes Dev (1998) 6.75
A possible role for RNA polymerase in the initiation of M13 DNA synthesis. Proc Natl Acad Sci U S A (1971) 6.71
Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin. Proc Natl Acad Sci U S A (1972) 5.74
Glycosylation and processing of prepro-alpha-factor through the yeast secretory pathway. Cell (1984) 5.53
Compartmentalized assembly of oligosaccharides on exported glycoproteins in yeast. Cell (1981) 5.51
Vesicle-mediated protein sorting. Annu Rev Biochem (1992) 5.20
A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum. J Cell Biol (1987) 4.94
Expression of genes in yeast using the ADCI promoter. Methods Enzymol (1983) 4.94
COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes. Cell (1998) 4.73
Reconstitution of SEC gene product-dependent intercompartmental protein transport. Cell (1988) 4.66
Lyticase: endoglucanase and protease activities that act together in yeast cell lysis. J Bacteriol (1980) 4.25
Invertase signal and mature sequence substitutions that delay intercompartmental transport of active enzyme. J Cell Biol (1985) 4.24
PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors. Mol Cell Biol (1986) 4.23
Functional compartments of the yeast Golgi apparatus are defined by the sec7 mutation. EMBO J (1989) 4.19
Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast. J Cell Biol (1989) 4.13
An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast. Proc Natl Acad Sci U S A (1983) 3.95
Localization of components involved in protein transport and processing through the yeast Golgi apparatus. J Cell Biol (1991) 3.86
Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y. J Cell Biol (1986) 3.85
Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae. J Cell Biol (1980) 3.84
Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature (1982) 3.82
Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Mol Biol Cell (1992) 3.81
Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product. Mol Cell Biol (1988) 3.74
Letrozole, a new oral aromatase inhibitor for advanced breast cancer: double-blind randomized trial showing a dose effect and improved efficacy and tolerability compared with megestrol acetate. J Clin Oncol (1998) 3.69
Multienzyme systems of DNA replication. Science (1974) 3.66
FAR1 links the signal transduction pathway to the cell cycle machinery in yeast. Cell (1993) 3.59
Protein localization and membrane traffic in yeast. Annu Rev Cell Biol (1985) 3.51
Membrane fusion and the cell cycle: Cdc48p participates in the fusion of ER membranes. Cell (1995) 3.44
A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast. J Cell Biol (1988) 3.37
Requirement for a GTPase-activating protein in vesicle budding from the endoplasmic reticulum. Science (1993) 3.36
STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes. Genes Dev (1989) 3.28
SEC12 encodes a guanine-nucleotide-exchange factor essential for transport vesicle budding from the ER. Nature (1993) 3.23
Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex. Nature (1991) 3.21
COPII-cargo interactions direct protein sorting into ER-derived transport vesicles. Nature (1998) 3.20
Randomised trial of intravenous immunoglobulin G, intravenous anti-D, and oral prednisone in childhood acute immune thrombocytopenic purpura. Lancet (1994) 3.12
Regulated import and degradation of a cytosolic protein in the yeast vacuole. Nature (1991) 3.12
Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p. Mol Cell Biol (1999) 3.04
SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer. Nature (1992) 2.99
Relation of breast cancer with passive and active exposure to tobacco smoke. Am J Epidemiol (1996) 2.93
Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1. Genes Dev (1992) 2.92
A population-based study of the usefulness of screening for neuroblastoma. Lancet (1997) 2.92
Sec61p mediates export of a misfolded secretory protein from the endoplasmic reticulum to the cytosol for degradation. EMBO J (1997) 2.92
COPI- and COPII-coated vesicles bud directly from the endoplasmic reticulum in yeast. Cell (1995) 2.87
Protein transport to the vacuole and receptor-mediated endocytosis by clathrin heavy chain-deficient yeast. J Cell Biol (1988) 2.86
Mcm1 is required to coordinate G2-specific transcription in Saccharomyces cerevisiae. Mol Cell Biol (1995) 2.85
GTP-binding Ypt1 protein and Ca2+ function independently in a cell-free protein transport reaction. Proc Natl Acad Sci U S A (1990) 2.78
Clathrin: a role in the intracellular retention of a Golgi membrane protein. Science (1989) 2.75
Forkhead-like transcription factors recruit Ndd1 to the chromatin of G2/M-specific promoters. Nature (2000) 2.74
Molecular characterization of the CAN1 locus in Saccharomyces cerevisiae. A transmembrane protein without N-terminal hydrophobic signal sequence. J Biol Chem (1985) 2.72
Neurological manifestations of intracranial dural arteriovenous malformations. J Neurosurg (1986) 2.72
Yeast secretory mutants that block the formation of active cell surface enzymes. J Cell Biol (1984) 2.71
A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription. Genes Dev (1991) 2.70
A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome. J Cell Biol (1993) 2.65
Yeast Sec23p acts in the cytoplasm to promote protein transport from the endoplasmic reticulum to the Golgi complex in vivo and in vitro. EMBO J (1989) 2.64
A primer on vesicle budding. Cell (1999) 2.58
Export of major cell surface proteins is blocked in yeast secretory mutants. J Cell Biol (1983) 2.58
Topology and functional domains of Sec63p, an endoplasmic reticulum membrane protein required for secretory protein translocation. Mol Cell Biol (1992) 2.51
Genetic and biochemical characterization of clathrin-deficient Saccharomyces cerevisiae. Mol Cell Biol (1987) 2.50
SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum. J Cell Biol (1989) 2.49
Ten proteins required for conversion of phiX174 single-stranded DNA to duplex form in vitro. Resolution and reconstitution. J Biol Chem (1975) 2.48
Kinase activity-dependent nuclear export opposes stress-induced nuclear accumulation and retention of Hog1 mitogen-activated protein kinase in the budding yeast Saccharomyces cerevisiae. Mol Biol Cell (1999) 2.46
Expression of a human gene for interferon in yeast. Nature (1981) 2.46
Dynamics of the COPII coat with GTP and stable analogues. Nat Cell Biol (2001) 2.44
A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. Proc Natl Acad Sci U S A (1973) 2.42
Structural and functional dissection of Sec62p, a membrane-bound component of the yeast endoplasmic reticulum protein import machinery. Mol Cell Biol (1990) 2.36
A test of clathrin function in protein secretion and cell growth. Science (1985) 2.36
SEC7 encodes an unusual, high molecular weight protein required for membrane traffic from the yeast Golgi apparatus. J Biol Chem (1988) 2.35
MAP kinase-related FUS3 from S. cerevisiae is activated by STE7 in vitro. Nature (1993) 2.33
MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae. EMBO J (1994) 2.32
GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae. J Bacteriol (1986) 2.30
Mammalian Sec23p homologue is restricted to the endoplasmic reticulum transitional cytoplasm. Proc Natl Acad Sci U S A (1991) 2.27
Actin from Saccharomyces cerevisiae. Mol Cell Biol (1982) 2.26
Stress-induced map kinase Hog1 is part of transcription activation complexes. Mol Cell (2001) 2.23
Incidence of silent hemorrhage and delayed deterioration after stereotactic brain biopsy. J Neurosurg (1998) 2.17
Coatomer, Arf1p, and nucleotide are required to bud coat protein complex I-coated vesicles from large synthetic liposomes. Proc Natl Acad Sci U S A (1998) 2.15
Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs. Science (1998) 2.14
Venous thromboembolic complications (VTE) in children: first analyses of the Canadian Registry of VTE. Blood (1994) 2.14
BiP and Sec63p are required for both co- and posttranslational protein translocation into the yeast endoplasmic reticulum. Proc Natl Acad Sci U S A (1995) 2.09
Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER. J Cell Biol (1991) 2.04
Purification and characterization of SAR1p, a small GTP-binding protein required for transport vesicle formation from the endoplasmic reticulum. J Biol Chem (1993) 2.04
The yeast SEC17 gene product is functionally equivalent to mammalian alpha-SNAP protein. J Biol Chem (1992) 2.03
Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases. Mol Cell Biol (1993) 2.03
ER export: public transportation by the COPII coach. Curr Opin Cell Biol (2001) 2.02
Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic Hsc70. J Cell Biol (1993) 2.02
Cytosolic Sec13p complex is required for vesicle formation from the endoplasmic reticulum in vitro. J Cell Biol (1993) 2.00
Genes required for completion of import of proteins into the endoplasmic reticulum in yeast. J Cell Biol (1984) 2.00
Significance of hemorrhage into brain tumors: clinicopathological study. J Neurosurg (1987) 1.95
Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. J Biol Chem (1994) 1.93
Invertase beta-galactosidase hybrid proteins fail to be transported from the endoplasmic reticulum in Saccharomyces cerevisiae. Mol Cell Biol (1984) 1.91
Chs6p-dependent anterograde transport of Chs3p from the chitosome to the plasma membrane in Saccharomyces cerevisiae. Mol Biol Cell (1998) 1.90
New mutants of Saccharomyces cerevisiae affected in the transport of proteins from the endoplasmic reticulum to the Golgi complex. Genetics (1996) 1.89