Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA.

PubWeight™: 7.11‹?› | Rank: Top 1%

🔗 View Article (PMC 433459)

Published in Proc Natl Acad Sci U S A on April 01, 1973

Authors

F Sanger, J E Donelson, A R Coulson, H Kössel, D Fischer

Articles citing this

Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A (1975) 56.75

DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res (1974) 19.42

Structure-independent nucleotide sequence analysis. Proc Natl Acad Sci U S A (1979) 8.65

Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis. Nucleic Acids Res (1978) 6.25

The use of nuclease P1 in sequence analysis of end group labeled RNA. Nucleic Acids Res (1977) 4.67

Ending the message: poly(A) signals then and now. Genes Dev (2011) 3.47

Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides. Nucleic Acids Res (1987) 3.24

The nucleotide sequence of formylmethionine tRNA from Mycoplasma mycoides sp. capri. Nucleic Acids Res (1978) 2.69

RNA sequencing with radioactive chain-terminating ribonucleotides. Proc Natl Acad Sci U S A (1978) 2.67

Nucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli. Proc Natl Acad Sci U S A (1977) 2.57

Nucleotide sequence in the promoter region of the Escherichia coli tyrosine tRNA gene. Proc Natl Acad Sci U S A (1974) 2.01

Nucleotide sequence of 5' terminus of alfalfa mosaic virus RNA 4 leading into coat protein cistron. Proc Natl Acad Sci U S A (1977) 1.99

Ten years of bacterial genome sequencing: comparative-genomics-based discoveries. Funct Integr Genomics (2006) 1.86

Comparative sequence analysis of the inverted terminal repetitions from different adenoviruses. Proc Natl Acad Sci U S A (1980) 1.75

The nucleotide sequence of the chloroplast 5S ribosomal RNA from spinach. Nucleic Acids Res (1981) 1.50

Homologous nucleotide sequences between prokaryotic and eukaryotic mRNAs: the 5'-end sequence of the mRNA of the lipoprotein of the Escherichia coli outer membrane. Proc Natl Acad Sci U S A (1978) 1.50

The nucleotide sequence of a major species of leucine tRNA from bovine liver. Nucleic Acids Res (1980) 1.49

Studies on transfer ribonucleic acids and related compounds. XLV. Block condensation of ribooligonucleotides containing 2'-O-tetrahydrofuranyl-5'-O-dimethoxytritylnucleosides. Nucleic Acids Res (1983) 1.49

ScrFI: a new sequence-specific endonuclease from Streptococcus cremoris. Nucleic Acids Res (1982) 1.45

Studies of gene control regions. III. Binding of synthetic and modified synthetic lac operator DNAs to lactose repressor. Nucleic Acids Res (1977) 1.44

Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleoside. Nucleic Acids Res (1985) 1.38

Synthesis of a gene for human growth hormone and its expression in Escherichia coli. Proc Natl Acad Sci U S A (1984) 1.34

Studies on gene control regions. VI. The 5- methyl of thymine, a lac repressor recognition site. Nucleic Acids Res (1977) 1.30

Primary structure of yeast mitochondrial DNA-coded phenylalanine-tRNA. Nucleic Acids Res (1978) 1.22

Nucleotide sequence of Scenedesmus obliquus cytoplasmic initiator tRNA. Nucleic Acids Res (1980) 1.20

Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent. Nucleic Acids Res (1982) 1.18

Application of fingerprinting techniques to iodinated nucleic acids. Proc Natl Acad Sci U S A (1973) 1.16

Studies on transfer ribonucleic acids and related compounds. XL. Synthesis of an eicosaribonucleotide corresponding to residues 35-54 of tRNAfMet from E. coli. Nucleic Acids Res (1981) 1.09

Nucleotide sequence of Neurospora crassa cytoplasmic initiator tRNA. Nucleic Acids Res (1977) 1.08

Sequences spanning the EcoRI substrate site. Nucleic Acids Res (1975) 1.04

Draft sequences of the radish (Raphanus sativus L.) genome. DNA Res (2014) 1.02

Extensive chromosome homoeology among Brassiceae species were revealed by comparative genetic mapping with high-density EST-based SNP markers in radish (Raphanus sativus L.). DNA Res (2011) 1.01

A new solid-phase synthesis of oligoribonucleotides by the phosphoro-p-anisidate method using tetrahydrofuranyl protection of 2'-hydroxyl groups. Nucleic Acids Res (1987) 0.96

The complete genome sequence of Thermoproteus tenax: a physiologically versatile member of the Crenarchaeota. PLoS One (2011) 0.95

Nucleotide sequence of HS-beta satellite DNA from kangaroo rat Dipodomys ordii. Proc Natl Acad Sci U S A (1973) 0.95

Selective 2'-benzoylation at the cis 2',3'-diols of protected ribonucleosides. New solid phase synthesis of RNA and DNA-RNA mixtures. Nucleic Acids Res (1982) 0.94

Reverse transcription of phage RNA and its fragment directed by synthetic heteropolymeric primers. Nucleic Acids Res (1977) 0.92

Synthesis of decadeoxyribonucleotides containing 5-modified uracils and their interactions with restriction endonucleases Bgl II, Sau 3AI and Mbo I (nucleosides and nucleotides 82). Nucleic Acids Res (1988) 0.87

Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction. Nucleic Acids Res (1979) 0.87

The sequence of sequencers: The history of sequencing DNA. Genomics (2015) 0.86

DNA sequence at the end of the cI gene in bacteriophage lambda. Nucleic Acids Res (1977) 0.86

A new insight into Sanger's development of sequencing: from proteins to DNA, 1943-1977. J Hist Biol (2010) 0.81

Next-Generation Sequencing Approaches in Cancer: Where Have They Brought Us and Where Will They Take Us? Cancers (Basel) (2015) 0.80

Research on neonatal microbiomes: what neonatologists need to know. Neonatology (2013) 0.80

The nucleotide sequence of phenylalanine tRNA from the cytoplasm of Neurospora crassa. Nucleic Acids Res (1980) 0.80

DNA sequence analysis: a formula to predict electrophoretic mobilities of oligonucleotides on cellulose acetate. Nucleic Acids Res (1974) 0.80

Synthesis and conformational studies of ribooligonucleotides which contain an alternating C-G sequence and show unusual circular dichroism spectra. Nucleic Acids Res (1984) 0.80

Determination of the sequences of 18 nucleotides from the 5'-end of the 1-strand and 15 nucleotides from the 5'-end of the r-strand of T7 DNA. Nucleic Acids Res (1975) 0.78

The nature of protein domain evolution: shaping the interaction network. Curr Genomics (2010) 0.77

Selective breakage of DNA alongside 5-bromodeoxyuridine nucleotide residues by high temperature hydrolysis. Nucleic Acids Res (1977) 0.77

Chemical synthesis of a primer and its use in the sequence analysis of the lysozyme gene of bacteriophage T4. Proc Natl Acad Sci U S A (1974) 0.76

Would Fred Sanger get funded today? Genetics (2014) 0.75

A rapid method for preparation of calf spleen exonuclease. Nucleic Acids Res (1977) 0.75

Romance of the three domains: how cladistics transformed the classification of cellular organisms. Protein Cell (2013) 0.75

Tetra-Primer ARMS PCR Optimization for Detection of IVS-II-I (G-A) and FSC 8/9 InsG Mutations in β-Thalassemia Major Patients in Isfahan Population. Iran J Public Health (2015) 0.75

Gene Transfer in Mycobacterium tuberculosis: Shuttle Phasmids to Enlightenment. Microbiol Spectr (2014) 0.75

Articles cited by this

A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol (1965) 20.83

Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem (1969) 15.21

The sequence of 5 s ribosomal ribonucleic acid. J Mol Biol (1968) 6.47

Nucleotide sequence analysis of DNA. II. Complete nucleotide sequence of the cohesive ends of bacteriophage lambda DNA. J Mol Biol (1971) 5.80

RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed? Nat New Biol (1972) 5.18

Active center of DNA polymerase. Science (1969) 4.47

Fractionation and sequences of the large pyrimidine oligonucleotides from bacteriophage fd DNA. J Mol Biol (1972) 4.42

Base sequence and evolution of guinea-pig alpha-satellite DNA. Nature (1970) 4.19

RESTRICTION OF IN VIVO GENETIC TRANSCRIPTION TO ONE OF THE COMPLEMENTARY STRANDS OF DNA. Proc Natl Acad Sci U S A (1963) 4.00

Determination of the nucleotide sequence of a fragment of bacteriophage phiX 174 DNA. Nat New Biol (1973) 3.74

Preparation of [alpha-32P]nucleoside and deoxynucleoside 5'-triphosphates from 32Pi and protected and unprotected nucleosides. Biochim Biophys Acta (1969) 3.25

[Virus proteins. IV. Constitution of the coat protein of the fd phage]. Hoppe Seylers Z Physiol Chem (1969) 2.56

Studies on polynucleotides. 118. A further study of ribonucleotide incorporation into deoxyribonucleic acid chains by deoxyribonucleic acid polymerase I of Escherichia coli. J Biol Chem (1972) 2.09

Nucleotide sequence analysis of DNA. IV. Complete nucleotide sequence of the left-hand cohesive end of coliphage 186 DNA. J Mol Biol (1972) 1.88

Sequence of the first 175 nucleotides from the 5' terminus of Qbeta RNA synthesized in vitro. Nature (1969) 1.84

Isolation and sequence analysis of a ribosome-protected fragment from bacteriophage phiX 174 DNA. Nat New Biol (1973) 1.79

Nucleotide sequence from the polypeptide chain termination region of the coat protein cistron in bacteriophage R17 RNA. Nature (1970) 1.71

A new approach to the sequence analysis of DNA. FEBS Lett (1972) 1.60

Nucleotide sequencing of DNA: preliminary characterization of the products of specific cleavages at guanine, cytosine, or adenine residues (bacteriophage M13-ribosubstitution-DNA polymerase I-electrophoresis-two-dimensional fingerprinting). Proc Natl Acad Sci U S A (1972) 1.44

The amino acid sequence of the B-protein of bacteriophage ZJ-2. Biochem J (1972) 1.36

Correlation between a coat protein amino-terminal sequence and a ribosome-binding DNA sequence from phiX 174. Nat New Biol (1973) 1.18

Articles by these authors

DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A (1977) 790.54

Nucleotide sequence of bacteriophage phi X174 DNA. Nature (1977) 95.69

A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol (1975) 90.35

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol (1980) 61.16

Nucleotide and amino acid sequences of gene G of omegaX174. J Mol Biol (1976) 57.73

Sequence and organization of the human mitochondrial genome. Nature (1981) 57.39

The use of thin acrylamide gels for DNA sequencing. FEBS Lett (1978) 40.52

A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster. Cell (1974) 27.33

A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol (1965) 20.83

Nucleotide sequence of bacteriophage lambda DNA. J Mol Biol (1982) 19.80

Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem (1969) 15.21

The nucleotide sequence of bacteriophage phiX174. J Mol Biol (1978) 13.55

The availability of the acetyl derivatives of lysine for growth. Biochem J (1943) 11.78

Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome. J Mol Biol (1982) 11.71

The free amino groups of haemoglobins. Biochem J (1948) 10.93

Nucleotide sequence of the yeast plasmid. Nature (1980) 7.64

The sequence of 5 s ribosomal ribonucleic acid. J Mol Biol (1968) 6.47

Determination of nucleotide sequences in DNA. Science (1981) 6.02

Primary and secondary structures of Escherichia coli MRE 600 23S ribosomal RNA. Comparison with models of secondary structure for maize chloroplast 23S rRNA and for large portions of mouse and human 16S mitochondrial rRNAs. Nucleic Acids Res (1981) 4.53

Nucleotide sequence of cro, cII and part of the O gene in phage lambda DNA. Nature (1978) 4.12

Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes. Nucleic Acids Res (1981) 4.04

Complete sequence of the maize chloroplast genome: gene content, hotspots of divergence and fine tuning of genetic information by transcript editing. J Mol Biol (1995) 3.92

MaxSub: an automated measure for the assessment of protein structure prediction quality. Bioinformatics (2000) 3.86

Different pattern of codon recognition by mammalian mitochondrial tRNAs. Proc Natl Acad Sci U S A (1980) 3.74

The RNA-binding protein HF-I, known as a host factor for phage Qbeta RNA replication, is essential for rpoS translation in Escherichia coli. Genes Dev (1996) 3.62

The metabolism of lysine. Biochem J (1944) 3.48

Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes. Cell (1981) 3.40

Effects of sevelamer and calcium-based phosphate binders on mortality in hemodialysis patients. Kidney Int (2007) 3.34

Nucleotide sequence of a Euglena gracilis chloroplast gene coding for the 16S rRNA: homologies to E. coli and Zea mays chloroplast 16S rRNA. Nucleic Acids Res (1982) 3.25

The rRNA operon from Zea mays chloroplasts: nucleotide sequence of 23S rDNA and its homology with E.coli 23S rDNA. Nucleic Acids Res (1981) 3.23

Nucleotide sequence from the coat protein cistron of R17 bacteriophage RNA. Nature (1969) 3.23

Synthetic polynucleotides. Enzymic synthesis of ribonucleotide terminated oligodeoxynucleotides and their use as primers for the enzymic synthesis of polydeoxynucleotides. Eur J Biochem (1971) 3.12

Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli. J Mol Biol (1967) 3.06

Structure of the rat prolactin gene. J Biol Chem (1980) 2.91

The response regulator RssB controls stability of the sigma(S) subunit of RNA polymerase in Escherichia coli. EMBO J (1996) 2.91

Osmotic regulation of rpoS-dependent genes in Escherichia coli. J Bacteriol (1993) 2.83

Rat hepatic cytosolic phosphoenolpyruvate carboxykinase (GTP). Structures of the protein, messenger RNA, and gene. J Biol Chem (1985) 2.82

Editing of a chloroplast mRNA by creation of an initiation codon. Nature (1991) 2.75

Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids. J Mol Biol (1969) 2.66

Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction. J Clin Microbiol (1989) 2.58

Role for the histone-like protein H-NS in growth phase-dependent and osmotic regulation of sigma S and many sigma S-dependent genes in Escherichia coli. J Bacteriol (1995) 2.55

Identification of transcriptional start sites and the role of ppGpp in the expression of rpoS, the structural gene for the sigma S subunit of RNA polymerase in Escherichia coli. J Bacteriol (1995) 2.54

Synthetic polynucleotides. Ther terminal addition of riboadenylic acid to deoxyoligonucleotides by terminal deoxynucleotidyl transferase as a tool for the specific labelling of deoxyoligonucleotides at the 3'-ends. Eur J Biochem (1971) 2.51

Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase. Proc Natl Acad Sci U S A (1989) 2.49

DNA base sequence of the po promoter region of phage lamdba. Nature (1977) 2.48

Sulindac causes regression of rectal polyps in familial adenomatous polyposis. Gastroenterology (1991) 2.43

Identification and molecular analysis of glgS, a novel growth-phase-regulated and rpoS-dependent gene involved in glycogen synthesis in Escherichia coli. Mol Microbiol (1992) 2.39

Reappraisal of the role of axillary lymph node dissection in the conservative treatment of breast cancer. J Clin Oncol (1997) 2.35

Increased incidence of Guillain-Barré syndrome after surgery. Eur J Neurol (2012) 2.32

The RNA-binding protein HF-I plays a global regulatory role which is largely, but not exclusively, due to its role in expression of the sigmaS subunit of RNA polymerase in Escherichia coli. J Bacteriol (1997) 2.31

Nucleotide sequence of 5S-ribosomal RNA from Escherichia coli. Nature (1967) 2.30

Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes. Nature (1980) 2.29

The rDNA of C. elegans: sequence and structure. Nucleic Acids Res (1986) 2.28

Detection of Trypanosoma congolense and Trypanosoma brucei subspecies by DNA amplification using the polymerase chain reaction. Parasitology (1989) 2.22

Functional analysis of the replicator structure of lambdoid bacteriophage DNAs. Cold Spring Harb Symp Quant Biol (1979) 2.18

Phosphohexosyl recognition is a general characteristic of pinocytosis of lysosomal glycosidases by human fibroblasts. J Clin Invest (1977) 2.18

RNA editing in plant mitochondria and chloroplasts. Plant Mol Biol (1996) 2.16

Introduction of a heterologous editing site into the tobacco plastid genome: the lack of RNA editing leads to a mutant phenotype. EMBO J (1994) 2.16

Double-stranded RNA interference in Trypanosoma brucei using head-to-head promoters. Mol Biochem Parasitol (2000) 2.15

The sequence of phenylalanine tRNA from E. coli. FEBS Lett (1969) 2.14

The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's. Science (1986) 2.14

DNA sequencing with chain-terminating inhibitors. 1977. Biotechnology (1992) 2.13

RNA editing in tobacco chloroplasts leads to the formation of a translatable psbL mRNA by a C to U substitution within the initiation codon. EMBO J (1992) 2.13

The organization and complete nucleotide sequence of the PstI restriction-modification system. J Biol Chem (1984) 2.07

The role of chest and abdominal computed tomography in assessing the severity of acute corrosive ingestion. Clin Toxicol (Phila) (2013) 2.06

Structure prediction meta server. Bioinformatics (2001) 2.02

3' End-group labeling and partial sequence determination of oligodeoxynucleotides. Methods Enzymol (1974) 2.00

Sequence of the Saccharomyces GAL region and its transcription in vivo. J Bacteriol (1984) 1.96

A DNA sequence analysis package for the IBM personal computer. Nucleic Acids Res (1984) 1.96

The nitrogen of the potato. Biochem J (1942) 1.94

A new method for the sequence analysis of oligodeoxynucleotides. Biochem Biophys Res Commun (1971) 1.89

In vivo dissection of cis-acting determinants for plastid RNA editing. EMBO J (1996) 1.88

Lorist2, a cosmid with transcriptional terminators insulating vector genes from interference by promoters within the insert: effect on DNA yield and cloned insert frequency. Gene (1987) 1.84

A computer vision based technique for 3-D sequence-independent structural comparison of proteins. Protein Eng (1993) 1.83

A gene coding for tRNA is located near 5' terminus of 16S rRNA gene in Zea mays chloroplast genome. Proc Natl Acad Sci U S A (1981) 1.81

LiveBench-1: continuous benchmarking of protein structure prediction servers. Protein Sci (2001) 1.80

Proofreading function of deoxyribonucleic acid polymerase I from Escherichia coli. Nature of excision of ribonucleotides from the 3' termini of oligodeoxynucleotide primers. J Biol Chem (1974) 1.80

Isolation and sequence analysis of a ribosome-protected fragment from bacteriophage phiX 174 DNA. Nat New Biol (1973) 1.79

Sequence analysis of specific areas of the 16S and 23S ribosomal RNAs. Nature (1968) 1.78

A study of quality measures for protein threading models. BMC Bioinformatics (2001) 1.74

Regulation of RssB-dependent proteolysis in Escherichia coli: a role for acetyl phosphate in a response regulator-controlled process. Mol Microbiol (1998) 1.74

Randomized comparison of high-frequency ventilation with high-rate intermittent positive pressure ventilation in preterm infants with respiratory failure. J Pediatr (1999) 1.69

LiveBench-2: large-scale automated evaluation of protein structure prediction servers. Proteins (2001) 1.68

Editing of the chloroplast rpoB transcript is independent of chloroplast translation and shows different patterns in barley and maize. EMBO J (1993) 1.68

Identification of editing positions in the ndhB transcript from maize chloroplasts reveals sequence similarities between editing sites of chloroplasts and plant mitochondria. Nucleic Acids Res (1992) 1.68

High-pathogenicity island of Yersinia pestis in enterobacteriaceae isolated from blood cultures and urine samples: prevalence and functional expression. J Infect Dis (2000) 1.66