Published in Nature on July 04, 1970
Genome engineering. Nat Biotechnol (2009) 4.60
Genetic assay for small fragments of bacteriophage phi X174 deoxyribonucleic acid. J Virol (1971) 3.78
Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase. Proc Natl Acad Sci U S A (1970) 3.11
Gene Designer: a synthetic biology tool for constructing artificial DNA segments. BMC Bioinformatics (2006) 2.85
Enzymatic oligomerization of bacteriophage P22 DNA and of linear Simian virus 40 DNA. Proc Natl Acad Sci U S A (1972) 2.62
Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res (2004) 2.26
Chemical synthesis of the mouse mitochondrial genome. Nat Methods (2010) 2.15
A study of the efficiency and the problem of sulfonation of several condensing reagents and their mechanisms for the chemical synthesis of deoxyoligoribonucleotides. Nucleic Acids Res (1980) 2.14
Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides. Nucleic Acids Res (2009) 1.70
Synthetic biology and the ethics of knowledge. J Med Ethics (2010) 1.58
Large-scale de novo DNA synthesis: technologies and applications. Nat Methods (2014) 1.51
Synthetic viruses: a new opportunity to understand and prevent viral disease. Nat Biotechnol (2009) 1.47
Rewriting the blueprint of life by synthetic genomics and genome engineering. Genome Biol (2015) 1.39
In vitro biosynthesis of pseudouridine at the polynucleotide level by an enzyme extract from Escherichia coli. Proc Natl Acad Sci U S A (1970) 1.25
Modeling structure-function relationships in synthetic DNA sequences using attribute grammars. PLoS Comput Biol (2009) 1.03
Putting synthesis into biology: a viral view of genetic engineering through de novo gene and genome synthesis. Chem Biol (2009) 1.01
The Saccharomyces cerevisiae SCRaMbLE system and genome minimization. Bioeng Bugs (2012) 1.00
A simple and accurate two-step long DNA sequences synthesis strategy to improve heterologous gene expression in pichia. PLoS One (2012) 0.87
Transcriptional origin of Euglena chloroplast tRNAs. Proc Natl Acad Sci U S A (1976) 0.86
A comprehensive list of chemically synthesized genes. Nucleic Acids Res (1988) 0.85
A new method for the synthesis of a structural gene. Nucleic Acids Res (1990) 0.85
Rational de novo gene synthesis by rapid polymerase chain assembly (PCA) and expression of endothelial protein-C and thrombin receptor genes. J Biotechnol (2007) 0.84
Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene. Nucleic Acids Res (1991) 0.83
The effect of antibiotics on the T4 polynucleotide ligase catalyzed template dependent polymerization of oligodeoxythymidylates. Nucleic Acids Res (1979) 0.83
Synthetic gene for the hepatitis C virus nucleocapsid protein. Nucleic Acids Res (1993) 0.78
DNA Oligonucleotide 3'-Phosphorylation by a DNA Enzyme. Biochemistry (2016) 0.77
Mega-cloning and the advent of synthetic genomes. Artif DNA PNA XNA (2010) 0.76
A theoretical model for template-free synthesis of long DNA sequence. Syst Synth Biol (2009) 0.75
Construction of a double-stranded deoxyribonucleotide sequence of 45 base pairs designed to code for S-peptide 2-14 of bovine ribonuclease A. Nucleic Acids Res (1975) 0.75
Four Inducible Promoters for Controlled Gene Expression in the Oleaginous Yeast Rhodotorula toruloides. Front Microbiol (2016) 0.75
Gene Assembly from Chip-Synthesized Oligonucleotides. Curr Protoc Chem Biol (2012) 0.75
CV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxyribonucleotide corresponding to the nucleotide sequence 31 to 50. J Mol Biol (1972) 58.52
Effect of salts and polyamines on T4 polynucleotide kinase. Biochemistry (1975) 22.01
Reversal of bacteriophage T4 induced polynucleotide kinase action. Biochemistry (1973) 21.41
Improved free-energy parameters for predictions of RNA duplex stability. Proc Natl Acad Sci U S A (1986) 19.52
Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry (1973) 11.45
Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature (1999) 9.87
Deficient signaling in mice devoid of double-stranded RNA-dependent protein kinase. EMBO J (1995) 6.94
A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules. Biochem Biophys Res Commun (1980) 6.64
Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin. Science (1996) 6.50
Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. Plant Cell (2000) 6.40
High-pressure liquid chromatography in polynucleotide synthesis. Biochemistry (1978) 5.98
Engineering cell shape and function. Science (1994) 5.95
Lumbar disc herniation. A controlled, prospective study with ten years of observation. Spine (Phila Pa 1976) (1983) 5.72
Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature (2000) 5.71
Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site. Nature (1982) 5.61
Structures of ternary complexes of rat DNA polymerase beta, a DNA template-primer, and ddCTP. Science (1994) 5.57
A novel proteolytic cleavage involved in Notch signaling: the role of the disintegrin-metalloprotease TACE. Mol Cell (2000) 5.53
Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA. Nature (1983) 5.18
IS431, a staphylococcal insertion sequence-like element related to IS26 from Proteus vulgaris. Gene (1987) 5.15
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells. Proc Natl Acad Sci U S A (1987) 5.11
Exclusion of elderly people from clinical research: a descriptive study of published reports. BMJ (1997) 4.95
Studies on the sequence of the 3'-terminal region of turnip-yellow-mosaic-virus RNA. Eur J Biochem (1977) 4.95
Vibrational spectroscopy of bacteriorhodopsin mutants: light-driven proton transport involves protonation changes of aspartic acid residues 85, 96, and 212. Biochemistry (1988) 4.93
Gene synthesis machines: DNA chemistry and its uses. Science (1985) 4.76
Regulation of lactate dehydrogenase and change of fermentation products in streptococci. J Bacteriol (1975) 4.60
Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor. Nature (2001) 4.55
In situ activation of bis-dialkylaminophosphines--a new method for synthesizing deoxyoligonucleotides on polymer supports. Nucleic Acids Res (1984) 4.55
Glutamic acid-113 serves as the retinylidene Schiff base counterion in bovine rhodopsin. Proc Natl Acad Sci U S A (1989) 4.44
An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions. J Biol Chem (1985) 4.44
A collision gradient method to determine the immersion depth of nitroxides in lipid bilayers: application to spin-labeled mutants of bacteriorhodopsin. Proc Natl Acad Sci U S A (1994) 4.27
Crystal structure of rat DNA polymerase beta: evidence for a common polymerase mechanism. Science (1994) 4.11
Complete nucleotide sequence of the 16S rRNA gene of Mycobacterium bovis BCG. J Bacteriol (1988) 4.10
Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants. Science (1990) 4.07
Initiation of haemoglobin synthesis by methionyl-tRNA. Nature (1970) 3.99
Three-dimensional structure of an oncogene protein: catalytic domain of human c-H-ras p21. Science (1988) 3.99
Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen. J Bacteriol (1988) 3.97
Small adenocarcinoma of the lung. Histologic characteristics and prognosis. Cancer (1995) 3.83
Studies on polynucleotides. LI. Syntheses of the 64 possible ribotrinucleotides derived from the four major ribomononucleotides. J Am Chem Soc (1966) 3.83
Amino acid sequence of bacteriorhodopsin. Proc Natl Acad Sci U S A (1979) 3.74
Studies on polynucleotides, XLIX. Stimulation of the binding of aminoacyl-sRNA's to ribosomes by ribotrinucleotides and a survey of codon assignments for 20 amino acids. Proc Natl Acad Sci U S A (1965) 3.71
Lumbar spinal stenosis: conservative or surgical management?: A prospective 10-year study. Spine (Phila Pa 1976) (2000) 3.69
Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons. Proc Natl Acad Sci U S A (1984) 3.67
Nucleotide sequence of three isoaccepting lysine tRNAs from rabbit liver and SV40-transformed mouse fibroblasts. Eur J Biochem (1979) 3.59
Plant defense in the absence of jasmonic acid: the role of cyclopentenones. Proc Natl Acad Sci U S A (2001) 3.58
Misreading of DNA templates containing 8-hydroxydeoxyguanosine at the modified base and at adjacent residues. Nature (1987) 3.55
The bacteriorhodopsin gene. Proc Natl Acad Sci U S A (1981) 3.40
A new form of amyloid protein associated with chronic hemodialysis was identified as beta 2-microglobulin. Biochem Biophys Res Commun (1985) 3.40
Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs. Proc Natl Acad Sci U S A (1973) 3.29
Structural studies on transmembrane proteins. 2. Spin labeling of bacteriorhodopsin mutants at unique cysteines. Biochemistry (1989) 3.25
Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides. Nucleic Acids Res (1987) 3.24
Apoptosis inhibitory activity of cytoplasmic p21(Cip1/WAF1) in monocytic differentiation. EMBO J (1999) 3.22
Polymer-supported RNA synthesis and its application to test the nearest-neighbor model for duplex stability. Biochemistry (1986) 3.21
Omalizumab, anti-IgE recombinant humanized monoclonal antibody, for the treatment of severe allergic asthma. J Allergy Clin Immunol (2001) 3.19
Aspartic acid substitutions affect proton translocation by bacteriorhodopsin. Proc Natl Acad Sci U S A (1988) 3.13
Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase. Proc Natl Acad Sci U S A (1970) 3.11
Total synthesis of a gene for bovine rhodopsin. Proc Natl Acad Sci U S A (1986) 3.08
Aspartic acid-96 is the internal proton donor in the reprotonation of the Schiff base of bacteriorhodopsin. Proc Natl Acad Sci U S A (1989) 3.06
Enzymatic Adenylylation of Streptomycin and Spectinomycin by R-Factor-Resistant Escherichia coli. Infect Immun (1970) 3.02
Prevalence and significance of low 25-hydroxyvitamin D concentrations in healthy subjects in Delhi. Am J Clin Nutr (2000) 3.02
Cysteine residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin. Proc Natl Acad Sci U S A (1988) 3.01
Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine. Proc Natl Acad Sci U S A (1985) 3.01
A strain-driven morphotropic phase boundary in BiFeO3. Science (2009) 2.95
The canals of Hering and hepatic stem cells in humans. Hepatology (1999) 2.93
Molecular cloning of a porcine gene syk that encodes a 72-kDa protein-tyrosine kinase showing high susceptibility to proteolysis. J Biol Chem (1991) 2.89
Traction therapy in sciatica due to disc prolapse (does traction treatment have any positive effect on patients suffering from sciatica caused by disc prolapse?). J Oslo City Hosp (1973) 2.89
Structure and function in rhodopsin. 7. Point mutations associated with autosomal dominant retinitis pigmentosa. Biochemistry (1994) 2.88
RNA splicing is required to make the messenger RNA for a variant surface antigen in trypanosomes. Nucleic Acids Res (1982) 2.87
Binding of synthetic lactose operator DNAs to lactose represessors. Proc Natl Acad Sci U S A (1977) 2.86
Search for a resonance decaying into WZ boson pairs in pp collisions. Phys Rev Lett (2010) 2.83
Molecular cloning and sequence determination of cDNAs for alpha subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain. Proc Natl Acad Sci U S A (1986) 2.83
Molecular cloning, expression, and gene localization of a fourth melanocortin receptor. J Biol Chem (1993) 2.81
Substitution of amino acids Asp-85, Asp-212, and Arg-82 in bacteriorhodopsin affects the proton release phase of the pump and the pK of the Schiff base. Proc Natl Acad Sci U S A (1990) 2.80
The anti-IgE antibody omalizumab reduces exacerbations and steroid requirement in allergic asthmatics. Eur Respir J (2001) 2.77
Bacterio-opsin mRNA in wild-type and bacterio-opsin-deficient Halobacterium halobium strains. Proc Natl Acad Sci U S A (1984) 2.77
A transposable element from Halobacterium halobium which inactivates the bacteriorhodopsin gene. Proc Natl Acad Sci U S A (1982) 2.76
Immediate early and early lytic cycle proteins are frequent targets of the Epstein-Barr virus-induced cytotoxic T cell response. J Exp Med (1997) 2.76
Association of B12 deficiency and clinical neuropathy with metformin use in type 2 diabetes patients. J Postgrad Med (2013) 2.75
Numbering system for the hammerhead. Nucleic Acids Res (1992) 2.74
Sequence-dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H. FEBS Lett (1987) 2.74
Nucleotide sequences at the 5'termini of rabbit alpha and beta globin mRNA. Cell (1976) 2.73
The cost of treating community-acquired pneumonia. Clin Ther (1998) 2.73
High-frequency spontaneous mutation in the bacterio-opsin gene in Halobacterium halobium is mediated by transposable elements. Proc Natl Acad Sci U S A (1983) 2.70
Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1. Science (1992) 2.70
Bacterial lipases: an overview of production, purification and biochemical properties. Appl Microbiol Biotechnol (2004) 2.69
Replacement of aspartic acid-96 by asparagine in bacteriorhodopsin slows both the decay of the M intermediate and the associated proton movement. Proc Natl Acad Sci U S A (1989) 2.64
Metal ion catalysis in the Tetrahymena ribozyme reaction. Nature (1993) 2.62
Cloning and characterization of the gene for immunogenic protein MPB64 of Mycobacterium bovis BCG. Infect Immun (1989) 2.62
Actinin-4, a novel actin-bundling protein associated with cell motility and cancer invasion. J Cell Biol (1998) 2.62
Enzymatic oligomerization of bacteriophage P22 DNA and of linear Simian virus 40 DNA. Proc Natl Acad Sci U S A (1972) 2.62