Published in Proc Natl Acad Sci U S A on February 01, 1986
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells. Proc Natl Acad Sci U S A (1987) 5.11
Glutamic acid-113 serves as the retinylidene Schiff base counterion in bovine rhodopsin. Proc Natl Acad Sci U S A (1989) 4.44
Cysteine residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin. Proc Natl Acad Sci U S A (1988) 3.01
The initiation of translation in E. coli: apparent base pairing between the 16srRNA and downstream sequences of the mRNA. Nucleic Acids Res (1990) 2.81
Structure and function in rhodopsin: high level expression of a synthetic bovine opsin gene and its mutants in stable mammalian cell lines. Proc Natl Acad Sci U S A (1996) 2.24
Role of the conserved NPxxY(x)5,6F motif in the rhodopsin ground state and during activation. Proc Natl Acad Sci U S A (2003) 2.21
Structure and function in rhodopsin: the role of asparagine-linked glycosylation. Proc Natl Acad Sci U S A (1994) 2.17
Repetitive DNA sequences in Mycoplasma pneumoniae. Nucleic Acids Res (1988) 2.09
G protein-coupled receptor rhodopsin: a prospectus. Annu Rev Physiol (2002) 2.08
Role of the intradiscal domain in rhodopsin assembly and function. Proc Natl Acad Sci U S A (1990) 1.97
Protonation states of membrane-embedded carboxylic acid groups in rhodopsin and metarhodopsin II: a Fourier-transform infrared spectroscopy study of site-directed mutants. Proc Natl Acad Sci U S A (1993) 1.97
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Physical mapping of the Mycoplasma pneumoniae genome. Nucleic Acids Res (1988) 1.92
Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy. Proc Natl Acad Sci U S A (1998) 1.77
Naturally occurring rhodopsin mutation in the dog causes retinal dysfunction and degeneration mimicking human dominant retinitis pigmentosa. Proc Natl Acad Sci U S A (2002) 1.67
Structure and function in rhodopsin: replacement by alanine of cysteine residues 110 and 187, components of a conserved disulfide bond in rhodopsin, affects the light-activated metarhodopsin II state. Proc Natl Acad Sci U S A (1994) 1.62
The role of the retinylidene Schiff base counterion in rhodopsin in determining wavelength absorbance and Schiff base pKa. Proc Natl Acad Sci U S A (1991) 1.38
Expression of the bacterioopsin gene in Halobacterium halobium using a multicopy plasmid. Proc Natl Acad Sci U S A (1991) 1.37
Sequence divergence in the ORF6 gene of Mycoplasma pneumonia. J Bacteriol (1994) 1.36
Two light-transducing membrane proteins: bacteriorhodopsin and the mammalian rhodopsin. Proc Natl Acad Sci U S A (1993) 1.32
Structure and function in rhodopsin: packing of the helices in the transmembrane domain and folding to a tertiary structure in the intradiscal domain are coupled. Proc Natl Acad Sci U S A (1997) 1.29
Transformation of normal rat kidney (NRK) cells by an infectious retrovirus carrying a synthetic rat type alpha transforming growth factor gene. Proc Natl Acad Sci U S A (1987) 1.27
Structure and function in rhodopsin: topology of the C-terminal polypeptide chain in relation to the cytoplasmic loops. Proc Natl Acad Sci U S A (1997) 1.25
Solid-phase assembly of cow colostrum trypsin inhibitor gene. Nucleic Acids Res (1987) 1.23
Structure and function in rhodopsin: correct folding and misfolding in point mutants at and in proximity to the site of the retinitis pigmentosa mutation Leu-125-->Arg in the transmembrane helix C. Proc Natl Acad Sci U S A (1996) 1.22
Expression of a bovine rhodopsin gene in Xenopus oocytes: demonstration of light-dependent ionic currents. Proc Natl Acad Sci U S A (1988) 1.20
In vivo assembly of rhodopsin from expressed polypeptide fragments. Proc Natl Acad Sci U S A (1995) 1.18
Crystal structure of a common GPCR-binding interface for G protein and arrestin. Nat Commun (2014) 1.16
Synthesis of a gene for sensory rhodopsin I and its functional expression in Halobacterium halobium. Proc Natl Acad Sci U S A (1993) 1.10
Vaccine-induced immune responses in rodents and nonhuman primates by use of a humanized human immunodeficiency virus type 1 pol gene. J Virol (2002) 1.07
Expression in bacteria of functional inhibitory subunit of retinal rod cGMP phosphodiesterase. Proc Natl Acad Sci U S A (1989) 1.06
Expression, stability, and membrane integration of truncation mutants of bovine rhodopsin. Proc Natl Acad Sci U S A (1997) 1.04
Construction of an EcoRI restriction map of Mycoplasma pneumoniae and localization of selected genes. J Bacteriol (1992) 1.04
Putting synthesis into biology: a viral view of genetic engineering through de novo gene and genome synthesis. Chem Biol (2009) 1.01
Synthesis and characterization of a novel retinylamine analog inhibitor of constitutively active rhodopsin mutants found in patients with autosomal dominant retinitis pigmentosa. Proc Natl Acad Sci U S A (1997) 0.90
Opsins with mutations at the site of chromophore attachment constitutively activate transducin but are not phosphorylated by rhodopsin kinase. Proc Natl Acad Sci U S A (1994) 0.89
An unstructured mRNA region and a 5' hairpin represent important elements of the E. coli translation initiation signal determined by using the bacteriophage T7 gene 1 translation start site. Nucleic Acids Res (1993) 0.87
How a small change in retinal leads to G-protein activation: initial events suggested by molecular dynamics calculations. Proteins (2007) 0.86
Mutations of the opsin gene (Y102H and I307N) lead to light-induced degeneration of photoreceptors and constitutive activation of phototransduction in mice. J Biol Chem (2010) 0.86
Ocular findings in a form of retinitis pigmentosa with a rhodopsin gene defect. Trans Am Ophthalmol Soc (1990) 0.86
A comprehensive list of chemically synthesized genes. Nucleic Acids Res (1988) 0.85
A new method for the synthesis of a structural gene. Nucleic Acids Res (1990) 0.85
Total synthesis and expression of a gene for the alpha-subunit of bovine rod outer segment guanine nucleotide-binding protein (transducin). Nucleic Acids Res (1988) 0.85
An orthogonal oligonucleotide protecting group strategy that enables assembly of repetitive or highly structured DNAs. Nucleic Acids Res (2002) 0.82
The design of synthetic genes. Nucleic Acids Res (1988) 0.82
Conformational selection and equilibrium governs the ability of retinals to bind opsin. J Biol Chem (2014) 0.80
Synthesis of a gene for human serum albumin and its expression in Saccharomyces cerevisiae. Nucleic Acids Res (1990) 0.79
Thermal stability of rhodopsin and progression of retinitis pigmentosa: comparison of S186W and D190N rhodopsin mutants. J Biol Chem (2013) 0.79
Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene. Nucleic Acids Res (1993) 0.78
Synthetic gene for the hepatitis C virus nucleocapsid protein. Nucleic Acids Res (1993) 0.78
The roles of transmembrane domain helix-III during rhodopsin photoactivation. PLoS One (2011) 0.78
Expression of bacteriorhodopsin in Sf9 and COS-1 cells. J Bioenerg Biomembr (1997) 0.77
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Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol (1980) 287.68
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene (1982) 59.01
Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res (1981) 32.85
Isolation, sequence analysis, and intron-exon arrangement of the gene encoding bovine rhodopsin. Cell (1983) 5.49
Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons. Proc Natl Acad Sci U S A (1984) 3.67
The structure of bovine rhodopsin. Biophys Struct Mech (1983) 3.33
Total synthesis of a gene. Science (1979) 2.38
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Reversal of bacteriophage T4 induced polynucleotide kinase action. Biochemistry (1973) 21.41
Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry (1973) 11.45
Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin. Science (1996) 6.50
High-pressure liquid chromatography in polynucleotide synthesis. Biochemistry (1978) 5.98
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells. Proc Natl Acad Sci U S A (1987) 5.11
Vibrational spectroscopy of bacteriorhodopsin mutants: light-driven proton transport involves protonation changes of aspartic acid residues 85, 96, and 212. Biochemistry (1988) 4.93
Glutamic acid-113 serves as the retinylidene Schiff base counterion in bovine rhodopsin. Proc Natl Acad Sci U S A (1989) 4.44
A collision gradient method to determine the immersion depth of nitroxides in lipid bilayers: application to spin-labeled mutants of bacteriorhodopsin. Proc Natl Acad Sci U S A (1994) 4.27
Hepatitis B virus nucleocapsid assembly: primary structure requirements in the core protein. J Virol (1990) 4.16
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Amino acid sequence of bacteriorhodopsin. Proc Natl Acad Sci U S A (1979) 3.74
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Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons. Proc Natl Acad Sci U S A (1984) 3.67
Effect of carboxylic acid side chains on the absorption maximum of visual pigments. Science (1989) 3.44
The bacteriorhodopsin gene. Proc Natl Acad Sci U S A (1981) 3.40
Structural studies on transmembrane proteins. 2. Spin labeling of bacteriorhodopsin mutants at unique cysteines. Biochemistry (1989) 3.25
Aspartic acid substitutions affect proton translocation by bacteriorhodopsin. Proc Natl Acad Sci U S A (1988) 3.13
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Aspartic acid-96 is the internal proton donor in the reprotonation of the Schiff base of bacteriorhodopsin. Proc Natl Acad Sci U S A (1989) 3.06
Cysteine residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin. Proc Natl Acad Sci U S A (1988) 3.01
Structure and function in rhodopsin. 7. Point mutations associated with autosomal dominant retinitis pigmentosa. Biochemistry (1994) 2.88
Constitutively active mutants of rhodopsin. Neuron (1992) 2.84
Substitution of amino acids Asp-85, Asp-212, and Arg-82 in bacteriorhodopsin affects the proton release phase of the pump and the pK of the Schiff base. Proc Natl Acad Sci U S A (1990) 2.80
Bacterio-opsin mRNA in wild-type and bacterio-opsin-deficient Halobacterium halobium strains. Proc Natl Acad Sci U S A (1984) 2.77
A transposable element from Halobacterium halobium which inactivates the bacteriorhodopsin gene. Proc Natl Acad Sci U S A (1982) 2.76
High-frequency spontaneous mutation in the bacterio-opsin gene in Halobacterium halobium is mediated by transposable elements. Proc Natl Acad Sci U S A (1983) 2.70
Replacement of aspartic acid-96 by asparagine in bacteriorhodopsin slows both the decay of the M intermediate and the associated proton movement. Proc Natl Acad Sci U S A (1989) 2.64
Total synthesis of the gene for an alanine transfer ribonucleic acid from yeast. Nature (1970) 2.51
Studies on polynucleotides, lxviii the primary structure of yeast phenylalanine transfer RNA. Proc Natl Acad Sci U S A (1967) 2.45
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A single amino acid substitution in rhodopsin (lysine 248----leucine) prevents activation of transducin. J Biol Chem (1988) 2.37
CIV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxynucleotide corresponding to the nucleotide sequence 21 to 40. J Mol Biol (1972) 2.35
Structure and function in rhodopsin. Measurement of the rate of metarhodopsin II decay by fluorescence spectroscopy. J Biol Chem (1995) 2.34
Structure and function in rhodopsin: high level expression of a synthetic bovine opsin gene and its mutants in stable mammalian cell lines. Proc Natl Acad Sci U S A (1996) 2.24
Orientation of bacteriorhodopsin in Halobacterium halobium as studied by selective proteolysis. Proc Natl Acad Sci U S A (1977) 2.23
Molecular determinants of human red/green color discrimination. Neuron (1994) 2.18
Assembly of functional rhodopsin requires a disulfide bond between cysteine residues 110 and 187. J Biol Chem (1990) 2.18
The nucleotide sequence in the promoter region of the fene for an Escherichia coli tyrosine transfer ribonucleic acid. J Biol Chem (1975) 2.17
Partial primary structure of bacteriorhodopsin: sequencing methods for membrane proteins. Proc Natl Acad Sci U S A (1979) 2.17
A visual pigment expressed in both rod and cone photoreceptors. Neuron (2001) 2.17
Structure and function in rhodopsin: the role of asparagine-linked glycosylation. Proc Natl Acad Sci U S A (1994) 2.17
Rhodopsin mutants that bind but fail to activate transducin. Science (1990) 2.16
GTPase of bovine rod outer segments: the amino acid sequence of the alpha subunit as derived from the cDNA sequence. Proc Natl Acad Sci U S A (1985) 2.09
Studies on polynucleotides. 118. A further study of ribonucleotide incorporation into deoxyribonucleic acid chains by deoxyribonucleic acid polymerase I of Escherichia coli. J Biol Chem (1972) 2.09
Glycerophospholipid synthesis: improved general method and new analogs containing photoactivable groups. Proc Natl Acad Sci U S A (1977) 2.07
Studies on polynucleotides. LXIX. Synthetic deoxyribopolynucleotides as templates for the DNA polymerase of Escherichia coli: DNA-like polymers containing repeating trinucleotide sequences. J Mol Biol (1967) 2.06
Replacement of aspartic residues 85, 96, 115, or 212 affects the quantum yield and kinetics of proton release and uptake by bacteriorhodopsin. Proc Natl Acad Sci U S A (1989) 2.04
The encapsidation signal on the hepatitis B virus RNA pregenome forms a stem-loop structure that is critical for its function. Nucleic Acids Res (1993) 2.04
Refolding of an integral membrane protein. Denaturation, renaturation, and reconstitution of intact bacteriorhodopsin and two proteolytic fragments. J Biol Chem (1981) 2.03
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Evolutionary conservation in the hepatitis B virus core structure: comparison of human and duck cores. Structure (1995) 1.98
Role of the intradiscal domain in rhodopsin assembly and function. Proc Natl Acad Sci U S A (1990) 1.97
Protonation state of Asp (Glu)-85 regulates the purple-to-blue transition in bacteriorhodopsin mutants Arg-82----Ala and Asp-85----Glu: the blue form is inactive in proton translocation. Proc Natl Acad Sci U S A (1990) 1.94
Heterozygous missense mutation in the rhodopsin gene as a cause of congenital stationary night blindness. Nat Genet (1993) 1.93
Mechanism of activation and inactivation of opsin: role of Glu113 and Lys296. Biochemistry (1992) 1.91
Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 1. General introduction. J Biol Chem (1976) 1.91
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Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: a site-directed spin-labeling study. Biochemistry (1996) 1.88
Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA's as catalyzed by DNA polymerases. J Mol Biol (1971) 1.87
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Structure of the lipopolysaccharide from an Escherichia coli heptose-less mutant. I. Chemical degradations and identification of products. J Biol Chem (1979) 1.86
Structure and function in rhodopsin. Studies of the interaction between the rhodopsin cytoplasmic domain and transducin. J Biol Chem (1992) 1.82
Mapping of the amino acids in membrane-embedded helices that interact with the retinal chromophore in bovine rhodopsin. J Biol Chem (1991) 1.80
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Structure and function in rhodopsin: replacement by alanine of cysteine residues 110 and 187, components of a conserved disulfide bond in rhodopsin, affects the light-activated metarhodopsin II state. Proc Natl Acad Sci U S A (1994) 1.62
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Structure and function in rhodopsin: correct folding and misfolding in two point mutants in the intradiscal domain of rhodopsin identified in retinitis pigmentosa. Proc Natl Acad Sci U S A (1996) 1.61
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Interferon gene transfer by a hepatitis B virus vector efficiently suppresses wild-type virus infection. Proc Natl Acad Sci U S A (1999) 1.49
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