Published in Gene on December 13, 1984
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics (1989) 105.30
A constant rate of spontaneous mutation in DNA-based microbes. Proc Natl Acad Sci U S A (1991) 11.66
Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes. Nucleic Acids Res (1986) 7.89
Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae. Mol Cell Biol (1988) 7.10
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc Natl Acad Sci U S A (1991) 6.82
Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Mol Cell Biol (1987) 6.67
Random-clone strategy for genomic restriction mapping in yeast. Proc Natl Acad Sci U S A (1986) 6.44
Characterization of double-strand break-induced recombination: homology requirements and single-stranded DNA formation. Mol Cell Biol (1992) 5.71
Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc Natl Acad Sci U S A (1989) 5.37
Four mating-type genes control sexual differentiation in the fission yeast. EMBO J (1988) 5.00
Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions. Proc Natl Acad Sci U S A (1996) 4.70
Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Mol Cell Biol (1986) 4.33
Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene. Mol Cell Biol (1986) 4.30
Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO J (1993) 4.27
Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1991) 4.18
Pathway correcting DNA replication errors in Saccharomyces cerevisiae. EMBO J (1993) 3.57
Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1987) 3.50
Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease. Proc Natl Acad Sci U S A (1989) 3.49
Analysis of meiosis-defective mutations in yeast by physical monitoring of recombination. Genetics (1986) 3.38
Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. Genetics (1986) 3.37
Constitutive and inducible Saccharomyces cerevisiae promoters: evidence for two distinct molecular mechanisms. Mol Cell Biol (1986) 3.37
Mutations in XRS2 and RAD50 delay but do not prevent mating-type switching in Saccharomyces cerevisiae. Mol Cell Biol (1994) 3.34
Role of Saccharomyces cerevisiae Msh2 and Msh3 repair proteins in double-strand break-induced recombination. Proc Natl Acad Sci U S A (1997) 3.29
Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1. Mol Cell Biol (1990) 3.24
Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J (1995) 3.23
Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. Mol Cell Biol (1986) 3.01
The structure and evolution of subtelomeric Y' repeats in Saccharomyces cerevisiae. Genetics (1992) 2.96
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p. J Cell Biol (1995) 2.95
Evolution of the dispersed SUC gene family of Saccharomyces by rearrangements of chromosome telomeres. Mol Cell Biol (1985) 2.88
Substrate length requirements for efficient mitotic recombination in Saccharomyces cerevisiae. Mol Cell Biol (1993) 2.81
Telomere-proximal DNA in Saccharomyces cerevisiae is refractory to methyltransferase activity in vivo. Proc Natl Acad Sci U S A (1992) 2.80
Role of conserved sequence elements in yeast centromere DNA. EMBO J (1985) 2.75
Timing of molecular events in meiosis in Saccharomyces cerevisiae: stable heteroduplex DNA is formed late in meiotic prophase. Mol Cell Biol (1993) 2.70
Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae. Mol Cell Biol (1987) 2.56
Altered expression of selectable marker URA3 in gene-disrupted Candida albicans strains complicates interpretation of virulence studies. Infect Immun (1998) 2.51
Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport. J Cell Biol (1995) 2.50
Length and distribution of meiotic gene conversion tracts and crossovers in Saccharomyces cerevisiae. Genetics (1989) 2.38
Physical lengths of meiotic and mitotic gene conversion tracts in Saccharomyces cerevisiae. Genetics (1988) 2.36
Specificity of mismatch repair following transformation of Saccharomyces cerevisiae with heteroduplex plasmid DNA. Proc Natl Acad Sci U S A (1989) 2.33
Characterization of a positive regulatory gene, LAC9, that controls induction of the lactose-galactose regulon of Kluyveromyces lactis: structural and functional relationships to GAL4 of Saccharomyces cerevisiae. Mol Cell Biol (1987) 2.29
Detection of heteroduplex DNA molecules among the products of Saccharomyces cerevisiae meiosis. Proc Natl Acad Sci U S A (1990) 2.26
Interaction of GAL4 and GAL80 gene regulatory proteins in vitro. Mol Cell Biol (1987) 2.26
Regulation of dimorphism in Saccharomyces cerevisiae: involvement of the novel protein kinase homolog Elm1p and protein phosphatase 2A. Mol Cell Biol (1993) 2.19
The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex. Mol Cell Biol (1995) 2.14
Interaction between cdc13+ and cdc2+ in the control of mitosis in fission yeast; dissociation of the G1 and G2 roles of the cdc2+ protein kinase. EMBO J (1987) 2.10
Adaptor complex-independent clathrin function in yeast. Mol Biol Cell (1999) 2.10
FLO11, a yeast gene related to the STA genes, encodes a novel cell surface flocculin. J Bacteriol (1996) 2.08
Effects of null mutations in the hexokinase genes of Saccharomyces cerevisiae on catabolite repression. Mol Cell Biol (1986) 2.06
Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid. J Bacteriol (1990) 2.02
Transcription-induced nucleosome 'splitting': an underlying structure for DNase I sensitive chromatin. EMBO J (1991) 1.93
SAM2 encodes the second methionine S-adenosyl transferase in Saccharomyces cerevisiae: physiology and regulation of both enzymes. Mol Cell Biol (1988) 1.87
Heteroduplex rejection during single-strand annealing requires Sgs1 helicase and mismatch repair proteins Msh2 and Msh6 but not Pms1. Proc Natl Acad Sci U S A (2004) 1.87
Chromatin transitions during activation and repression of galactose-regulated genes in yeast. EMBO J (1993) 1.84
Fine-resolution mapping of spontaneous and double-strand break-induced gene conversion tracts in Saccharomyces cerevisiae reveals reversible mitotic conversion polarity. Mol Cell Biol (1994) 1.84
Characterization of RPR1, an essential gene encoding the RNA component of Saccharomyces cerevisiae nuclear RNase P. Mol Cell Biol (1991) 1.82
Saccharomyces cerevisiae contains two functional citrate synthase genes. Mol Cell Biol (1986) 1.82
Identification of two factors which bind to the upstream sequences of a number of nuclear genes coding for mitochondrial proteins and to genetic elements important for cell division in yeast. Nucleic Acids Res (1988) 1.80
cis- and trans-acting regulatory elements of the yeast URA3 promoter. Mol Cell Biol (1990) 1.74
Synapsis-mediated fusion of free DNA ends forms inverted dimer plasmids in yeast. Genetics (1990) 1.65
A transcription map of a yeast centromere plasmid: unexpected transcripts and altered gene expression. Nucleic Acids Res (1985) 1.62
Ty1 transposition in Saccharomyces cerevisiae is nonrandom. Genetics (1989) 1.60
GCD14p, a repressor of GCN4 translation, cooperates with Gcd10p and Lhp1p in the maturation of initiator methionyl-tRNA in Saccharomyces cerevisiae. Mol Cell Biol (1999) 1.59
Isolation and characterization of a Saccharomyces cerevisiae peptide transport gene. Mol Cell Biol (1994) 1.49
Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity. EMBO J (1993) 1.49
SnR30: a new, essential small nuclear RNA from Saccharomyces cerevisiae. Nucleic Acids Res (1988) 1.48
Meiotic recombination between repeated transposable elements in Saccharomyces cerevisiae. Mol Cell Biol (1988) 1.46
The 'polysemous' codon--a codon with multiple amino acid assignment caused by dual specificity of tRNA identity. EMBO J (1997) 1.45
A new type of fusion analysis applicable to many organisms: protein fusions to the URA3 gene of yeast. Genetics (1987) 1.42
Modification and transfer into an embryonal carcinoma cell line of a 360-kilobase human-derived yeast artificial chromosome. Mol Cell Biol (1990) 1.41
An in vivo assay for the reverse transcriptase of human retrotransposon L1 in Saccharomyces cerevisiae. Mol Cell Biol (1994) 1.38
Homologous recombination between single-stranded DNA and chromosomal genes in Saccharomyces cerevisiae. Mol Cell Biol (1987) 1.33
Vertebrate and yeast calmodulin, despite significant sequence divergence, are functionally interchangeable. Proc Natl Acad Sci U S A (1989) 1.29
Unusual enhancer function in yeast rRNA transcription. Mol Cell Biol (1989) 1.29
Functional analysis of mRNA 3' end formation signals in the convergent and overlapping transcription units of the S. cerevisiae genes RHO1 and MRP2. Nucleic Acids Res (1993) 1.28
Repeated use of GAL1 for gene disruption in Candida albicans. Genetics (1991) 1.25
A yeast cyclophilin gene essential for lactate metabolism at high temperature. Proc Natl Acad Sci U S A (1992) 1.23
General amino acid control and specific arginine repression in Saccharomyces cerevisiae: physical study of the bifunctional regulatory region of the ARG3 gene. Mol Cell Biol (1985) 1.22
Sequences required for transcriptional initiation of the Saccharomyces cerevisiae CYC7 genes. Mol Cell Biol (1987) 1.22
Effects of excess centromeres and excess telomeres on chromosome loss rates. Mol Cell Biol (1991) 1.19
The Saccharomyces cerevisiae mutation elm4-1 facilitates pseudohyphal differentiation and interacts with a deficiency in phosphoribosylpyrophosphate synthase activity to cause constitutive pseudohyphal growth. Mol Cell Biol (1994) 1.16
The Saccharomyces cerevisiae ACP2 gene encodes an essential HMG1-like protein. Mol Cell Biol (1988) 1.15
The RAD5 gene product is involved in the avoidance of non-homologous end-joining of DNA double strand breaks in the yeast Saccharomyces cerevisiae. Nucleic Acids Res (1997) 1.15
Regulation of STA1 gene expression by MAT during the life cycle of Saccharomyces cerevisiae. Mol Cell Biol (1989) 1.15
SARs stimulate but do not confer position independent gene expression. Nucleic Acids Res (1994) 1.13
Use of polymerase chain reaction for rapid detection of gene insertions in whole yeast cells. Nucleic Acids Res (1991) 1.13
Isolation and characterization of mutations in the HXK2 gene of Saccharomyces cerevisiae. Mol Cell Biol (1989) 1.12
Separation-of-function mutations in Saccharomyces cerevisiae MSH2 that confer mismatch repair defects but do not affect nonhomologous-tail removal during recombination. Mol Cell Biol (1999) 1.08
Casein kinase II mediates multiple phosphorylation of Saccharomyces cerevisiae eIF-2 alpha (encoded by SUI2), which is required for optimal eIF-2 function in S. cerevisiae. Mol Cell Biol (1994) 1.04
The Saccharomyces SHP1 gene, which encodes a regulator of phosphoprotein phosphatase 1 with differential effects on glycogen metabolism, meiotic differentiation, and mitotic cell cycle progression. Mol Cell Biol (1995) 1.03
Ethanol formation in adh0 mutants reveals the existence of a novel acetaldehyde-reducing activity in Saccharomyces cerevisiae. J Bacteriol (1990) 1.03
Molecular cloning and nucleotide sequence for the complete coding region of human UMP synthase. Proc Natl Acad Sci U S A (1988) 1.00
Sequence of the Kluyveromyces lactis URA3 gene. Nucleic Acids Res (1987) 1.00
Molecular analysis of Saccharomyces cerevisiae chromosome I: identification of additional transcribed regions and demonstration that some encode essential functions. Genetics (1991) 0.99
Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts. Microb Cell Fact (2006) 0.97
Identification and characterization of FAR3, a gene required for pheromone-mediated G1 arrest in Saccharomyces cerevisiae. Genetics (1996) 0.95
Targeted alterations in yeast artificial chromosomes for inter-species gene transfer. Nucleic Acids Res (1992) 0.95
High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl Environ Microbiol (1994) 0.93
The general control activator protein GCN4 is essential for a basal level of ARO3 gene expression in Saccharomyces cerevisiae. Mol Cell Biol (1989) 0.91
Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet (2000) 336.52
Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A (1998) 192.97
Molecular portraits of human breast tumours. Nature (2000) 94.14
Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics (1989) 79.44
Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet (1980) 75.25
Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications. Proc Natl Acad Sci U S A (2001) 71.82
Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature (2000) 64.32
Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell (1998) 62.20
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The transcriptional program of sporulation in budding yeast. Science (1998) 37.23
Genomic expression programs in the response of yeast cells to environmental changes. Mol Biol Cell (2000) 36.09
The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature (1997) 33.47
Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase. Cell (1982) 25.10
Systematic variation in gene expression patterns in human cancer cell lines. Nat Genet (2000) 24.22
Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF. Nature (2001) 21.55
Genetic and physical maps of Saccharomyces cerevisiae. Nature (1997) 21.48
Missing value estimation methods for DNA microarrays. Bioinformatics (2001) 20.84
Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. J Mol Biol (1977) 20.29
SGD: Saccharomyces Genome Database. Nucleic Acids Res (1998) 20.26
A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene (1987) 19.68
Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene (1979) 19.39
The transcriptional program in the response of human fibroblasts to serum. Science (1999) 19.19
Exploring the new world of the genome with DNA microarrays. Nat Genet (1999) 19.05
Integrating functional genomic information into the Saccharomyces genome database. Nucleic Acids Res (2000) 18.52
Singular value decomposition for genome-wide expression data processing and modeling. Proc Natl Acad Sci U S A (2000) 18.42
Diversity of gene expression in adenocarcinoma of the lung. Proc Natl Acad Sci U S A (2001) 17.36
A common language for physical mapping of the human genome. Science (1989) 17.36
Genome-wide analysis of DNA copy-number changes using cDNA microarrays. Nat Genet (1999) 14.96
Distinctive gene expression patterns in human mammary epithelial cells and breast cancers. Proc Natl Acad Sci U S A (1999) 14.16
Using the Saccharomyces Genome Database (SGD) for analysis of protein similarities and structure. Nucleic Acids Res (1999) 13.65
A gene expression database for the molecular pharmacology of cancer. Nat Genet (2000) 13.31
Promoter-specific binding of Rap1 revealed by genome-wide maps of protein-DNA association. Nat Genet (2001) 13.05
Comparative genomics of Listeria species. Science (2001) 12.94
Homozygosity mapping: a way to map human recessive traits with the DNA of inbred children. Science (1987) 12.20
Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol (1975) 11.14
Strategies for studying heterogeneous genetic traits in humans by using a linkage map of restriction fragment length polymorphisms. Proc Natl Acad Sci U S A (1986) 9.51
Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell (1985) 8.53
Mapping complex genetic traits in humans: new methods using a complete RFLP linkage map. Cold Spring Harb Symp Quant Biol (1986) 8.22
The Stanford Microarray Database. Nucleic Acids Res (2001) 7.77
The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery. Cell (1988) 7.39
Plasmid construction by homologous recombination in yeast. Gene (1987) 7.26
Diverse effects of beta-tubulin mutations on microtubule formation and function. J Cell Biol (1988) 6.88
Lethal disruption of the yeast actin gene by integrative DNA transformation. Science (1982) 6.88
Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli. Proc Natl Acad Sci U S A (1979) 6.81
Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda. Genetics (1978) 6.73
Genomic expression responses to DNA-damaging agents and the regulatory role of the yeast ATR homolog Mec1p. Mol Biol Cell (2001) 6.39
Comparison of the complete protein sets of worm and yeast: orthology and divergence. Science (1998) 6.28
Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo. Cell (1983) 6.22
Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate. Virology (1972) 6.21
Mutants of yeast defective in sucrose utilization. Genetics (1981) 6.20
DNA topoisomerase II is required at the time of mitosis in yeast. Cell (1985) 6.08
EIR1, a root-specific protein involved in auxin transport, is required for gravitropism in Arabidopsis thaliana. Genes Dev (1998) 6.06
Yeast actin-binding proteins: evidence for a role in morphogenesis. J Cell Biol (1988) 5.46
Systematic changes in gene expression patterns following adaptive evolution in yeast. Proc Natl Acad Sci U S A (1999) 5.45
Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene. Mol Gen Genet (1984) 5.44
'Gene shaving' as a method for identifying distinct sets of genes with similar expression patterns. Genome Biol (2000) 5.29
A rapid, efficient method for isolating DNA from yeast. Gene (1986) 5.20
Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane. J Cell Biol (1994) 5.14
Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana. Nature (1999) 5.03
Secretion of beta-lactamase requires the carboxy end of the protein. Cell (1980) 5.03
Systematic mutational analysis of the yeast ACT1 gene. Genetics (1992) 5.02
Simple Mendelian inheritance of the reiterated ribosomal DNA of yeast. Proc Natl Acad Sci U S A (1977) 4.86
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Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast. Proc Natl Acad Sci U S A (1981) 4.79
Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics (1982) 4.66
Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast. Methods Enzymol (1983) 4.63
Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth. Nature (2000) 4.52
Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins. Proc Natl Acad Sci U S A (1975) 4.52
Molecular genetics of bacteriophage P22. Microbiol Rev (1978) 4.51
A group of interacting yeast DNA replication genes. Genes Dev (1991) 4.45
Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Mol Cell Biol (1986) 4.33
Many random sequences functionally replace the secretion signal sequence of yeast invertase. Science (1987) 4.33
Genome-wide characterization of the Zap1p zinc-responsive regulon in yeast. Proc Natl Acad Sci U S A (2000) 4.30
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ADP-ribosylation factor is functionally and physically associated with the Golgi complex. Proc Natl Acad Sci U S A (1990) 4.12
The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence. Mol Cell Biol (1983) 3.96
Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I. Nature (1990) 3.90
Suppressors of yeast actin mutations. Genetics (1989) 3.87
Propagation of some human DNA sequences in bacteriophage lambda vectors requires mutant Escherichia coli hosts. Proc Natl Acad Sci U S A (1985) 3.82
A suppressor of SNF1 mutations causes constitutive high-level invertase synthesis in yeast. Genetics (1984) 3.81
Isolation and characterization of mutations in the beta-tubulin gene of Saccharomyces cerevisiae. Genetics (1985) 3.77
Host/vector interactions which affect the viability of recombinant phage lambda clones. Gene (1986) 3.75
WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and aberrantly expressed in human colon tumors. Proc Natl Acad Sci U S A (1998) 3.73
Requirement of yeast fimbrin for actin organization and morphogenesis in vivo. Nature (1991) 3.70
Subcellular localization of Cdc42p, a Saccharomyces cerevisiae GTP-binding protein involved in the control of cell polarity. Mol Biol Cell (1993) 3.70
Genetic analysis of the yeast cytoskeleton. Annu Rev Genet (1987) 3.67
Adult T-cell lymphoma-leukaemia in Blacks from the West Indies. Lancet (1982) 3.67
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High-throughput genotyping with single nucleotide polymorphisms. Genome Res (2001) 3.55
Complete DNA sequence of yeast chromosome XI. Nature (1994) 3.51
Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene. Proc Natl Acad Sci U S A (1984) 3.47
Unlinked noncomplementation: isolation of new conditional-lethal mutations in each of the tubulin genes of Saccharomyces cerevisiae. Genetics (1988) 3.43
Synthesis and maturation of phage P22 DNA. I. Identification of intermediates. J Mol Biol (1968) 3.39
Subcellular localization of yeast CDC46 varies with the cell cycle. Genes Dev (1990) 3.34
Movement of yeast cortical actin cytoskeleton visualized in vivo. Proc Natl Acad Sci U S A (1996) 3.32
A gene required for the separation of chromosomes on the spindle apparatus in yeast. Cell (1986) 3.32