Published in Nature on November 16, 2000
A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci U S A (2001) 33.71
The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics. PLoS Biol (2003) 13.32
Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J (2001) 12.88
A functional genomic analysis of cell morphology using RNA interference. J Biol (2003) 5.99
Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor. Nucleic Acids Res (2002) 5.93
Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res (2004) 5.81
Informatics and quantitative analysis in biological imaging. Science (2003) 5.22
Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions. PLoS Biol (2003) 5.07
Stable suppression of gene expression by RNAi in mammalian cells. Proc Natl Acad Sci U S A (2002) 5.05
A comprehensive evolutionary classification of proteins encoded in complete eukaryotic genomes. Genome Biol (2004) 4.94
Similarities and differences in genome-wide expression data of six organisms. PLoS Biol (2003) 4.75
Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (2010) 4.69
Identification of genes needed for regeneration, stem cell function, and tissue homeostasis by systematic gene perturbation in planaria. Dev Cell (2005) 4.35
WormBase: a multi-species resource for nematode biology and genomics. Nucleic Acids Res (2004) 4.14
CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex. J Cell Biol (2005) 3.54
Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics (2001) 3.49
Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms. Science (2004) 3.48
Reverse genetics in the mosquito Anopheles gambiae: targeted disruption of the Defensin gene. EMBO Rep (2002) 3.43
A first version of the Caenorhabditis elegans Promoterome. Genome Res (2004) 3.29
KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. Genes Dev (2003) 3.29
Automatic identification of subcellular phenotypes on human cell arrays. Genome Res (2004) 3.25
High-throughput gene mapping in Caenorhabditis elegans. Genome Res (2002) 3.21
A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans. J Cell Biol (2005) 3.18
Terminal cytokinesis events uncovered after an RNAi screen. Curr Biol (2004) 2.99
Prp8 protein: at the heart of the spliceosome. RNA (2005) 2.89
Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proc Natl Acad Sci U S A (2002) 2.85
The GAPs, GEFs, and GDIs of heterotrimeric G-protein alpha subunits. Int J Biol Sci (2005) 2.76
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Nature (2009) 2.68
Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. J Cell Biol (2006) 2.67
A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue. Cell (2011) 2.64
Genomewide view of gene silencing by small interfering RNAs. Proc Natl Acad Sci U S A (2003) 2.56
Astral signals spatially bias cortical myosin recruitment to break symmetry and promote cytokinesis. Curr Biol (2007) 2.44
CDK-9/cyclin T (P-TEFb) is required in two postinitiation pathways for transcription in the C. elegans embryo. Genes Dev (2002) 2.36
Requirement for a conserved Toll/interleukin-1 resistance domain protein in the Caenorhabditis elegans immune response. Proc Natl Acad Sci U S A (2004) 2.23
A complex of LIN-5 and GPR proteins regulates G protein signaling and spindle function in C elegans. Genes Dev (2003) 2.23
RNA interference: biology, mechanism, and applications. Microbiol Mol Biol Rev (2003) 2.19
Identification of in vivo mRNA targets of GLD-1, a maxi-KH motif containing protein required for C. elegans germ cell development. Genes Dev (2001) 2.19
Drosophila asterless and vertebrate Cep152 Are orthologs essential for centriole duplication. Genetics (2008) 2.17
Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo. Mol Biol Cell (2003) 2.12
Analysis and functional classification of transcripts from the nematode Meloidogyne incognita. Genome Biol (2003) 2.10
Building the centriole. Curr Biol (2010) 2.07
siRNA relieves chronic neuropathic pain. Nucleic Acids Res (2004) 1.96
MILI, a PIWI-interacting RNA-binding protein, is required for germ line stem cell self-renewal and appears to positively regulate translation. J Biol Chem (2008) 1.93
A Caenorhabditis elegans model of insulin resistance: altered macronutrient storage and dauer formation in an OGT-1 knockout. Proc Natl Acad Sci U S A (2005) 1.93
Use of cDNA subtraction and RNA interference screens in combination reveals genes required for germ-line development in Caenorhabditis elegans. Proc Natl Acad Sci U S A (2001) 1.88
"Holo"er than thou: chromosome segregation and kinetochore function in C. elegans. Chromosome Res (2004) 1.88
Automated microscopy for high-content RNAi screening. J Cell Biol (2010) 1.87
Roles for 147 embryonic lethal genes on C.elegans chromosome I identified by RNA interference and video microscopy. EMBO J (2001) 1.85
Vigilance and validation: Keys to success in RNAi screening. ACS Chem Biol (2010) 1.85
Applications of high-throughput RNA interference screens to problems in cell and developmental biology. Genetics (2007) 1.82
Establishment of a tissue-specific RNAi system in C. elegans. Gene (2007) 1.82
RNAiDB and PhenoBlast: web tools for genome-wide phenotypic mapping projects. Nucleic Acids Res (2004) 1.74
The Arabidopsis SeedGenes Project. Nucleic Acids Res (2003) 1.73
Worm Phenotype Ontology: integrating phenotype data within and beyond the C. elegans community. BMC Bioinformatics (2011) 1.69
Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos. Mol Biol Cell (2002) 1.69
E-RNAi: a web application to design optimized RNAi constructs. Nucleic Acids Res (2005) 1.69
WorfDB: the Caenorhabditis elegans ORFeome Database. Nucleic Acids Res (2003) 1.65
MES-4: an autosome-associated histone methyltransferase that participates in silencing the X chromosomes in the C. elegans germ line. Development (2006) 1.63
Using RNA interference to identify genes required for RNA interference. Proc Natl Acad Sci U S A (2002) 1.63
The Tre2 (USP6) oncogene is a hominoid-specific gene. Proc Natl Acad Sci U S A (2003) 1.62
Functional and phylogenetic analysis of the ubiquitylation system in Caenorhabditis elegans: ubiquitin-conjugating enzymes, ubiquitin-activating enzymes, and ubiquitin-like proteins. Genome Biol (2001) 1.61
Schizosaccharomyces pombe essential genes: a pilot study. Genome Res (2003) 1.58
Conservation and functional significance of gene topology in the genome of Caenorhabditis elegans. Genome Res (2006) 1.56
The ABC transporter gene family of Caenorhabditis elegans has implications for the evolutionary dynamics of multidrug resistance in eukaryotes. Genome Biol (2004) 1.55
Toxicogenomic analysis of Caenorhabditis elegans reveals novel genes and pathways involved in the resistance to cadmium toxicity. Genome Biol (2007) 1.55
Roles of formin nodes and myosin motor activity in Mid1p-dependent contractile-ring assembly during fission yeast cytokinesis. Mol Biol Cell (2009) 1.52
RNAi microarray analysis in cultured mammalian cells. Genome Res (2003) 1.51
An integrated strategy to study muscle development and myofilament structure in Caenorhabditis elegans. PLoS Genet (2009) 1.50
Phosducin-Like Protein 3 is required for microtubule-dependent steps of cell division but not for meristem growth in Arabidopsis. Plant Cell (2008) 1.44
RNA interference of the salivary gland nitrophorin 2 in the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae) by dsRNA ingestion or injection. Insect Biochem Mol Biol (2006) 1.43
Exon-specific RNAi: a tool for dissecting the functional relevance of alternative splicing. RNA (2002) 1.41
Profiling of the mammalian mitotic spindle proteome reveals an ER protein, OSTD-1, as being necessary for cell division and ER morphology. PLoS One (2013) 1.40
TILLING is an effective reverse genetics technique for Caenorhabditis elegans. BMC Genomics (2006) 1.40
Inducible systemic RNA silencing in Caenorhabditis elegans. Mol Biol Cell (2003) 1.38
Stable and controllable RNA interference: Investigating the physiological function of glutathionylated actin. Proc Natl Acad Sci U S A (2003) 1.36
A targeted RNAi screen for genes involved in chromosome morphogenesis and nuclear organization in the Caenorhabditis elegans germline. Genetics (2002) 1.35
Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails. Genome Biol (2005) 1.35
Here, there, and everywhere: kinetochore function on holocentric chromosomes. J Cell Biol (2001) 1.35
UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. Dev Biol (2009) 1.32
C. elegans La-related protein, LARP-1, localizes to germline P bodies and attenuates Ras-MAPK signaling during oogenesis. RNA (2008) 1.32
Explosive lineage-specific expansion of the orphan nuclear receptor HNF4 in nematodes. J Mol Evol (2005) 1.31
Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution. Genome Biol (2006) 1.30
Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function. Mol Biol Cell (2006) 1.26
Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in Caenorhabditis elegans embryonic polarity. BMC Dev Biol (2003) 1.26
Effects on RNAi of the tight structure, sequence and position of the targeted region. Nucleic Acids Res (2004) 1.26
Statins inhibit protein lipidation and induce the unfolded protein response in the non-sterol producing nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A (2009) 1.26
A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans. Nucleic Acids Res (2006) 1.25
Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA. Proc Natl Acad Sci U S A (2002) 1.23
large-scale screening for targeted knockouts in the Caenorhabditis elegans genome. G3 (Bethesda) (2012) 1.23
Haspin-like proteins: a new family of evolutionarily conserved putative eukaryotic protein kinases. Protein Sci (2001) 1.21
Comparative genomics of gene expression in the parasitic and free-living nematodes Strongyloides stercoralis and Caenorhabditis elegans. Genome Res (2004) 1.20
Minimizing off-target effects by using diced siRNAs for RNA interference. J RNAi Gene Silencing (2006) 1.20
Efficient target-selected mutagenesis in Caenorhabditis elegans: toward a knockout for every gene. Genome Res (2007) 1.20
Multiple subunits of the Caenorhabditis elegans anaphase-promoting complex are required for chromosome segregation during meiosis I. Genetics (2002) 1.19
High-throughput analysis of spatio-temporal dynamics in Dictyostelium. Genome Biol (2007) 1.19
Cytoplasmic dynein light intermediate chain is required for discrete aspects of mitosis in Caenorhabditis elegans. Mol Biol Cell (2001) 1.18
Small interfering RNA production by enzymatic engineering of DNA (SPEED). Proc Natl Acad Sci U S A (2004) 1.17
A cell cycle timer for asymmetric spindle positioning. PLoS Biol (2009) 1.16
A survey of new temperature-sensitive, embryonic-lethal mutations in C. elegans: 24 alleles of thirteen genes. PLoS One (2011) 1.15
SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery. Mol Biol Cell (2008) 1.13
Initial sequencing and analysis of the human genome. Nature (2001) 212.86
Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol (1993) 45.22
SMART, a simple modular architecture research tool: identification of signaling domains. Proc Natl Acad Sci U S A (1998) 36.83
Comparative genomics of the eukaryotes. Science (2000) 26.62
Variable number of tandem repeat (VNTR) markers for human gene mapping. Science (1987) 25.71
Prediction of deleterious human alleles. Hum Mol Genet (2001) 21.00
A multicenter, randomized, controlled clinical trial of transfusion requirements in critical care. Transfusion Requirements in Critical Care Investigators, Canadian Critical Care Trials Group. N Engl J Med (1999) 20.31
SMART: a web-based tool for the study of genetically mobile domains. Nucleic Acids Res (2000) 17.77
HGBASE: a database of SNPs and other variations in and around human genes. Nucleic Acids Res (2000) 11.75
BRCA1 protein products ... Functional motifs... Nat Genet (1996) 11.50
SMART: identification and annotation of domains from signalling and extracellular protein sequences. Nucleic Acids Res (1999) 11.33
Conservation of gene order: a fingerprint of proteins that physically interact. Trends Biochem Sci (1998) 9.94
Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans. Nature (2005) 9.50
Meta-analysis of 74,046 individuals identifies 11 new susceptibility loci for Alzheimer's disease. Nat Genet (2013) 9.46
Gene families: the taxonomy of protein paralogs and chimeras. Science (1997) 8.80
Genome phylogeny based on gene content. Nat Genet (1999) 8.12
The DNA sequence and comparative analysis of human chromosome 20. Nature (2002) 7.40
Measuring genome evolution. Proc Natl Acad Sci U S A (1998) 6.97
QTc-interval abnormalities and psychotropic drug therapy in psychiatric patients. Lancet (2000) 6.78
Quantitative phylogenetic assessment of microbial communities in diverse environments. Science (2007) 6.35
A functional genomic analysis of cell morphology using RNA interference. J Biol (2003) 5.99
A global profile of germline gene expression in C. elegans. Mol Cell (2000) 5.97
An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region. Genetics (1999) 5.87
STRING: a web-server to retrieve and display the repeatedly occurring neighbourhood of a gene. Nucleic Acids Res (2000) 5.68
Metabolism and evolution of Haemophilus influenzae deduced from a whole-genome comparison with Escherichia coli. Curr Biol (1996) 5.50
Horizontal transfer of multiple penicillin-binding protein genes, and capsular biosynthetic genes, in natural populations of Streptococcus pneumoniae. Mol Microbiol (1991) 5.45
Towards a structural basis of human non-synonymous single nucleotide polymorphisms. Trends Genet (2000) 5.41
Predicting protein function by genomic context: quantitative evaluation and qualitative inferences. Genome Res (2000) 5.14
Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau. Mol Biol Cell (1992) 5.11
EST comparison indicates 38% of human mRNAs contain possible alternative splice forms. FEBS Lett (2000) 4.96
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts. Cell (1990) 4.92
Functional analysis of kinetochore assembly in Caenorhabditis elegans. J Cell Biol (2001) 4.82
The DNA sequence and analysis of human chromosome 6. Nature (2003) 4.75
Epidemiological evidence of the spread of a Mycobacterium tuberculosis strain of the Beijing genotype on Gran Canaria Island. Am J Respir Crit Care Med (2001) 4.75
Rab5 regulates motility of early endosomes on microtubules. Nat Cell Biol (1999) 4.74
The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast. Genes Dev (1999) 4.69
A survey of expressed genes in Caenorhabditis elegans. Nat Genet (1992) 4.63
eggNOG v2.0: extending the evolutionary genealogy of genes with enhanced non-supervised orthologous groups, species and functional annotations. Nucleic Acids Res (2009) 4.55
Differential genome display. Trends Genet (1997) 4.48
Positionally cloned human disease genes: patterns of evolutionary conservation and functional motifs. Proc Natl Acad Sci U S A (1997) 4.40
A novel class of RanGTP binding proteins. J Cell Biol (1997) 4.36
Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. Nat Cell Biol (2000) 4.31
Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo. Nature (2001) 4.15
REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export. RNA (2000) 4.10
HGVbase: a human sequence variation database emphasizing data quality and a broad spectrum of data sources. Nucleic Acids Res (2002) 4.08
First clinical isolate of vancomycin-intermediate Staphylococcus aureus in a French hospital. Lancet (1998) 4.08
Identification of essential components of the S. cerevisiae kinetochore. Cell (1993) 4.03
Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. EMBO J (2000) 3.97
Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo. J Cell Biol (1999) 3.97
Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans. J Cell Biol (2001) 3.86
SNP frequencies in human genes an excess of rare alleles and differing modes of selection. Trends Genet (2000) 3.78
Comparison of ARM and HEAT protein repeats. J Mol Biol (2001) 3.78
Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus. Cell (1986) 3.77
Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant J (1998) 3.75
Software for genome mapping by fingerprinting techniques. Comput Appl Biosci (1988) 3.67
Is a low transfusion threshold safe in critically ill patients with cardiovascular diseases? Crit Care Med (2001) 3.65
Genetics of resistance to third-generation cephalosporins in clinical isolates of Streptococcus pneumoniae. Mol Microbiol (1992) 3.58
CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis. J Cell Biol (2000) 3.55
Meiotic recombination, noncoding DNA and genomic organization in Caenorhabditis elegans. Genetics (1995) 3.45
Eukaryotic signalling domain homologues in archaea and bacteria. Ancient ancestry and horizontal gene transfer. J Mol Biol (1999) 3.28
Automated extraction of information in molecular biology. FEBS Lett (2000) 3.28
HEAT repeats in the Huntington's disease protein. Nat Genet (1995) 3.24
Early-onset autosomal dominant Alzheimer disease: prevalence, genetic heterogeneity, and mutation spectrum. Am J Hum Genet (1999) 3.14
Flower colour intensity depends on specialized cell shape controlled by a Myb-related transcription factor. Nature (1994) 3.13
Binding of the adenomatous polyposis coli protein to microtubules increases microtubule stability and is regulated by GSK3 beta phosphorylation. Curr Biol (2001) 3.13
Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol (1999) 3.12
Non-orthologous gene displacement. Trends Genet (1996) 3.12
Computer support for interpreting family histories of breast and ovarian cancer in primary care: comparative study with simulated cases. BMJ (2000) 3.12
Characterization of a novel protein-binding module--the WW domain. FEBS Lett (1995) 3.11
5-Fluorouracil versus 5-fluorouracil plus alpha-interferon as treatment of metastatic colorectal carcinoma. A randomized study. Ann Oncol (1996) 3.10
Protein annotation: detective work for function prediction. Trends Genet (1998) 3.04
Characterization of the mammalian YAP (Yes-associated protein) gene and its role in defining a novel protein module, the WW domain. J Biol Chem (1995) 3.01
Mott transition in VO2 revealed by infrared spectroscopy and nano-imaging. Science (2007) 3.01
Flavonoid synthesis in Petunia hybrida: partial characterization of dihydroflavonol-4-reductase genes. Plant Mol Biol (1989) 3.00
Evolution of domain families. Adv Protein Chem (2000) 2.98
Prediction of potential GPI-modification sites in proprotein sequences. J Mol Biol (1999) 2.95
Yeast chromosome III: new gene functions. EMBO J (1994) 2.85
Reconstitution of physiological microtubule dynamics using purified components. Science (2001) 2.83
Measurements of energetic particle radiation in transit to Mars on the Mars Science Laboratory. Science (2013) 2.83
SPRY domains in ryanodine receptors (Ca(2+)-release channels). Trends Biochem Sci (1997) 2.82
Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma. Proc Natl Acad Sci U S A (1998) 2.80
Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames. Nucleic Acids Res (2000) 2.79
Dyspepsia in England and Scotland. Gut (1990) 2.73