Published in Proc Natl Acad Sci U S A on December 21, 2005
The Bacillus subtilis endospore: assembly and functions of the multilayered coat. Nat Rev Microbiol (2012) 2.30
Recent progress in Bacillus subtilis sporulation. FEMS Microbiol Rev (2011) 2.12
Dynamics of spore coat morphogenesis in Bacillus subtilis. Mol Microbiol (2011) 1.81
Genomic determinants of sporulation in Bacilli and Clostridia: towards the minimal set of sporulation-specific genes. Environ Microbiol (2012) 1.59
Hierarchical evolution of the bacterial sporulation network. Curr Biol (2010) 1.50
Role of dipicolinic acid in the germination, stability, and viability of spores of Bacillus subtilis. J Bacteriol (2008) 1.19
Spore formation in Bacillus subtilis. Environ Microbiol Rep (2013) 1.19
Role of spore coat proteins in the resistance of Bacillus subtilis spores to Caenorhabditis elegans predation. J Bacteriol (2008) 1.18
A genomic signature and the identification of new sporulation genes. J Bacteriol (2013) 1.12
The Tetrahymena thermophila phagosome proteome. Eukaryot Cell (2006) 1.11
CotC-CotU heterodimerization during assembly of the Bacillus subtilis spore coat. J Bacteriol (2007) 1.08
The coat morphogenetic protein SpoVID is necessary for spore encasement in Bacillus subtilis. Mol Microbiol (2009) 1.04
Characterization of spores of Bacillus subtilis that lack most coat layers. J Bacteriol (2008) 1.04
Roles of the Bacillus anthracis spore protein ExsK in exosporium maturation and germination. J Bacteriol (2009) 1.00
CotE binds to CotC and CotU and mediates their interaction during spore coat formation in Bacillus subtilis. J Bacteriol (2009) 0.99
Vacuolar protein sorting protein 13A, TtVPS13A, localizes to the tetrahymena thermophila phagosome membrane and is required for efficient phagocytosis. Eukaryot Cell (2011) 0.96
Interaction between coat morphogenetic proteins SafA and SpoVID. J Bacteriol (2006) 0.94
Analysis of the effects of a gerP mutation on the germination of spores of Bacillus subtilis. J Bacteriol (2012) 0.94
The timing of cotE expression affects Bacillus subtilis spore coat morphology but not lysozyme resistance. J Bacteriol (2006) 0.93
Flexibility of the programme of spore coat formation in Bacillus subtilis: bypass of CotE requirement by over-production of CotH. PLoS One (2013) 0.92
Efficient inhibition of germination of coat-deficient bacterial spores by multivalent metal cations, including terbium (Tb³+). Appl Environ Microbiol (2011) 0.92
Physical interaction between coat morphogenetic proteins SpoVID and CotE is necessary for spore encasement in Bacillus subtilis. J Bacteriol (2012) 0.88
Predation by Myxococcus xanthus induces Bacillus subtilis to form spore-filled megastructures. Appl Environ Microbiol (2014) 0.88
Analysis of dye binding by and membrane potential in spores of Bacillus species. J Appl Microbiol (2009) 0.88
Genome Diversity of Spore-Forming Firmicutes. Microbiol Spectr (2013) 0.87
Role of the gerP operon in germination and outgrowth of Bacillus anthracis spores. PLoS One (2010) 0.86
Protozoal digestion of coat-defective Bacillus subtilis spores produces "rinds" composed of insoluble coat protein. Appl Environ Microbiol (2008) 0.85
Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy. J Bacteriol (2008) 0.84
Conservation and innovation in Tetrahymena membrane traffic: proteins, lipids, and compartments. Methods Cell Biol (2012) 0.82
Genomics, evolution, and crystal structure of a new family of bacterial spore kinases. Proteins (2010) 0.81
Architecture and assembly of the Bacillus subtilis spore coat. PLoS One (2014) 0.81
Mechanism of Bacillus subtilis spore inactivation by and resistance to supercritical CO2 plus peracetic acid. J Appl Microbiol (2015) 0.80
Sporulation during growth in a gut isolate of Bacillus subtilis. J Bacteriol (2014) 0.80
Diverse supramolecular structures formed by self-assembling proteins of the Bacillus subtilis spore coat. Mol Microbiol (2015) 0.80
Clostridium perfringens Sporulation and Sporulation-Associated Toxin Production. Microbiol Spectr (2016) 0.78
Sporulation Temperature Reveals a Requirement for CotE in the Assembly of both the Coat and Exosporium Layers of Bacillus cereus Spores. Appl Environ Microbiol (2015) 0.77
The Bacillus subtilis germinant receptor GerA triggers premature germination in response to morphological defects during sporulation. Mol Microbiol (2017) 0.76
Structural and genetic analysis of X-ray scattering by spores of Bacillus subtilis. J Bacteriol (2009) 0.76
Contrasting evolutionary patterns of spore coat proteins in two Bacillus species groups are linked to a difference in cellular structure. BMC Evol Biol (2013) 0.76
Bacterial Sphingomyelinases and Phospholipases as Virulence Factors. Microbiol Mol Biol Rev (2016) 0.76
Surviving Between Hosts: Sporulation and Transmission. Microbiol Spectr (2016) 0.75
Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores. J Bacteriol (2015) 0.75
The Conserved Spore Coat Protein SpoVM Is Largely Dispensable in Clostridium difficile Spore Formation. mSphere (2017) 0.75
Effects of Bacillus cereus Endospores on Free-Living Protist Growth. Microb Ecol (2016) 0.75
Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol Biol Rev (2000) 6.86
Spore germination. Curr Opin Microbiol (2003) 6.02
A bacteriolytic agent that detects and kills Bacillus anthracis. Nature (2002) 5.63
Revival and identification of bacterial spores in 25- to 40-million-year-old Dominican amber. Science (1995) 4.59
Bacillus subtilis spore coat. Microbiol Mol Biol Rev (1999) 4.21
Role of ger proteins in nutrient and nonnutrient triggering of spore germination in Bacillus subtilis. J Bacteriol (2000) 3.79
Isolation of a 250 million-year-old halotolerant bacterium from a primary salt crystal. Nature (2000) 3.75
Characterization of spores of Bacillus subtilis which lack dipicolinic acid. J Bacteriol (2000) 3.50
A modified reagent for dipicolinic acid analysis. Anal Biochem (1968) 3.15
Early Bacillus anthracis-macrophage interactions: intracellular survival survival and escape. Cell Microbiol (2000) 3.11
Genetic requirements for induction of germination of spores of Bacillus subtilis by Ca(2+)-dipicolinate. J Bacteriol (2001) 2.97
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis. J Mol Biol (2003) 2.59
Proteomic analysis of the spore coats of Bacillus subtilis and Bacillus anthracis. J Bacteriol (2003) 2.34
Fate of germinated Bacillus anthracis spores in primary murine macrophages. Mol Microbiol (2001) 2.30
Maximum shields: the assembly and function of the bacterial spore coat. Trends Microbiol (2002) 1.90
Cloning and characterization of a cluster of genes encoding polypeptides present in the insoluble fraction of the spore coat of Bacillus subtilis. J Bacteriol (1993) 1.82
Mechanisms of killing of Bacillus subtilis spores by hypochlorite and chlorine dioxide. J Appl Microbiol (2003) 1.81
The products of the spoVA operon are involved in dipicolinic acid uptake into developing spores of Bacillus subtilis. J Bacteriol (2002) 1.72
Involvement of superoxide dismutase in spore coat assembly in Bacillus subtilis. J Bacteriol (1998) 1.68
Proteomics characterization of novel spore proteins of Bacillus subtilis. Microbiology (2002) 1.61
Bacillus subtilis spore coat assembly requires cotH gene expression. J Bacteriol (1996) 1.61
Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate. J Bacteriol (2003) 1.55
Preservation records of micro-organisms: evidence of the tenacity of life. Microbiology (1994) 1.55
Localization of the cortex lytic enzyme CwlJ in spores of Bacillus subtilis. J Bacteriol (2002) 1.54
Interactions among CotB, CotG, and CotH during assembly of the Bacillus subtilis spore coat. J Bacteriol (2004) 1.46
Cloning, DNA sequence, functional analysis and transcriptional regulation of the genes encoding dipicolinic acid synthetase required for sporulation in Bacillus subtilis. J Mol Biol (1993) 1.46
Alkyl hydroperoxide reductase, catalase, MrgA, and superoxide dismutase are not involved in resistance of Bacillus subtilis spores to heat or oxidizing agents. J Bacteriol (1997) 1.41
Assembly of multiple CotC forms into the Bacillus subtilis spore coat. J Bacteriol (2004) 1.34
Dynamic patterns of subcellular protein localization during spore coat morphogenesis in Bacillus subtilis. J Bacteriol (2004) 1.29
Assembly requirements and role of CotH during spore coat formation in Bacillus subtilis. J Bacteriol (1999) 1.21
Transient and stable DNA transformation of Tetrahymena thermophila by electroporation. Methods Cell Biol (2000) 1.19
Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores. J Bacteriol (2004) 1.14
On the fate of ingested Bacillus spores. Res Microbiol (2000) 1.11
Novel method to control pathogenic bacteria on human mucous membranes. Ann N Y Acad Sci (2003) 1.11
Transglutaminase in sporulating cells of Bacillus subtilis. J Gen Appl Microbiol (1998) 0.99
Effect of mechanical abrasion on the viability, disruption and germination of spores of Bacillus subtilis. J Appl Microbiol (2005) 0.97
Germination, growth, and sporulation of Bacillus thuringiensis subsp. israelensis in excreted food vacuoles of the protozoan Tetrahymena pyriformis. Appl Environ Microbiol (1998) 0.93
A Dictyostelium mutant with reduced lysozyme levels compensates by increased phagocytic activity. J Biol Chem (2005) 0.92
Three pools of lysosomal enzymes in Tetrahymena thermophila. Exp Cell Res (1993) 0.84
Spores of Bacillus thuringiensis serovar israelensis as tracers for ingestion rates by Tetrahymena pyriformis. J Invertebr Pathol (1994) 0.82
From rings to layers: surprising patterns of protein deposition during bacterial spore assembly. J Bacteriol (2004) 0.82
Secretion of tetrain, a Tetrahymena cysteine protease, as a mature enzyme and its identification as a member of the cathepsin L subfamily. Eur J Biochem (1998) 0.80
The role of secreted acid hydrolases in the utilization of complex nutrients byTetrahymena. Microb Ecol (1990) 0.77
The forespore line of gene expression in Bacillus subtilis. J Mol Biol (2006) 2.52
Germination of spores of Bacillales and Clostridiales species: mechanisms and proteins involved. Trends Microbiol (2010) 2.24
Effects of overexpression of nutrient receptors on germination of spores of Bacillus subtilis. J Bacteriol (2003) 2.21
Cooperativity between different nutrient receptors in germination of spores of Bacillus subtilis and reduction of this cooperativity by alterations in the GerB receptor. J Bacteriol (2006) 1.87
The products of the spoVA operon are involved in dipicolinic acid uptake into developing spores of Bacillus subtilis. J Bacteriol (2002) 1.72
Studies of the commitment step in the germination of spores of bacillus species. J Bacteriol (2010) 1.68
Isolation and characterization of superdormant spores of Bacillus species. J Bacteriol (2009) 1.65
Role of SpoVA proteins in release of dipicolinic acid during germination of Bacillus subtilis spores triggered by dodecylamine or lysozyme. J Bacteriol (2006) 1.57
Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate. J Bacteriol (2003) 1.55
Localization of the cortex lytic enzyme CwlJ in spores of Bacillus subtilis. J Bacteriol (2002) 1.54
Characterization of Clostridium perfringens spores that lack SpoVA proteins and dipicolinic acid. J Bacteriol (2008) 1.53
Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile. Proc Natl Acad Sci U S A (2004) 1.52
Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster. Mol Microbiol (2011) 1.49
How moist heat kills spores of Bacillus subtilis. J Bacteriol (2007) 1.48
Role of GerD in germination of Bacillus subtilis spores. J Bacteriol (2006) 1.44
A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: implications for spore dormancy. Proc Natl Acad Sci U S A (2003) 1.43
Localization of SpoVAD to the inner membrane of spores of Bacillus subtilis. J Bacteriol (2005) 1.42
Mechanisms of induction of germination of Bacillus subtilis spores by high pressure. Appl Environ Microbiol (2002) 1.42
Effects of a gerF (lgt) mutation on the germination of spores of Bacillus subtilis. J Bacteriol (2004) 1.35
Levels of Ca2+-dipicolinic acid in individual bacillus spores determined using microfluidic Raman tweezers. J Bacteriol (2007) 1.30
The solar UV environment and bacterial spore UV resistance: considerations for Earth-to-Mars transport by natural processes and human spaceflight. Mutat Res (2005) 1.30
Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics. Anal Chem (2009) 1.29
Factors influencing germination of Bacillus subtilis spores via activation of nutrient receptors by high pressure. Appl Environ Microbiol (2005) 1.28
Factors affecting variability in time between addition of nutrient germinants and rapid dipicolinic acid release during germination of spores of Bacillus species. J Bacteriol (2010) 1.27
Structure of a protein-DNA complex essential for DNA protection in spores of Bacillus species. Proc Natl Acad Sci U S A (2008) 1.27
Analysis of the germination of spores of Bacillus subtilis with temperature sensitive spo mutations in the spoVA operon. FEMS Microbiol Lett (2004) 1.25
Clostridium perfringens spore germination: characterization of germinants and their receptors. J Bacteriol (2007) 1.24
Combination of Raman tweezers and quantitative differential interference contrast microscopy for measurement of dynamics and heterogeneity during the germination of individual bacterial spores. J Biomed Opt (2010) 1.23
SleC is essential for cortex peptidoglycan hydrolysis during germination of spores of the pathogenic bacterium Clostridium perfringens. J Bacteriol (2009) 1.22
Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins. J Bacteriol (2006) 1.21
Interaction between individual protein components of the GerA and GerB nutrient receptors that trigger germination of Bacillus subtilis spores. J Bacteriol (2005) 1.19
Role of dipicolinic acid in the germination, stability, and viability of spores of Bacillus subtilis. J Bacteriol (2008) 1.19
Investigating the role of small, acid-soluble spore proteins (SASPs) in the resistance of Clostridium perfringens spores to heat. BMC Microbiol (2006) 1.19
Germination of individual Bacillus subtilis spores with alterations in the GerD and SpoVA proteins, which are important in spore germination. J Bacteriol (2011) 1.15
Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores. Appl Environ Microbiol (2012) 1.14
Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores. J Bacteriol (2004) 1.14
Role of a SpoVA protein in dipicolinic acid uptake into developing spores of Bacillus subtilis. J Bacteriol (2012) 1.13
Superdormant spores of Bacillus species have elevated wet-heat resistance and temperature requirements for heat activation. J Bacteriol (2009) 1.13
Antisense-RNA-mediated decreased synthesis of small, acid-soluble spore proteins leads to decreased resistance of clostridium perfringens spores to moist heat and UV radiation. Appl Environ Microbiol (2007) 1.12
Gene expression in Bacillus subtilis surface biofilms with and without sporulation and the importance of yveR for biofilm maintenance. Biotechnol Bioeng (2004) 1.12
Characterization of bacterial spore germination using phase-contrast and fluorescence microscopy, Raman spectroscopy and optical tweezers. Nat Protoc (2011) 1.12
Localization of the germination protein GerD to the inner membrane in Bacillus subtilis spores. J Bacteriol (2008) 1.12
Germination protein levels and rates of germination of spores of Bacillus subtilis with overexpressed or deleted genes encoding germination proteins. J Bacteriol (2012) 1.11
The physical state of water in bacterial spores. Proc Natl Acad Sci U S A (2009) 1.09
Analysis of interactions between nutrient germinant receptors and SpoVA proteins of Bacillus subtilis spores. FEMS Microbiol Lett (2007) 1.08
Levels of germination proteins in dormant and superdormant spores of Bacillus subtilis. J Bacteriol (2012) 1.07
Photosensitization of DNA by dipicolinic acid, a major component of spores of Bacillus species. Photochem Photobiol Sci (2005) 1.07
The protease CspB is essential for initiation of cortex hydrolysis and dipicolinic acid (DPA) release during germination of spores of Clostridium perfringens type A food poisoning isolates. Microbiology (2009) 1.07
Release of small molecules during germination of spores of Bacillus Species. J Bacteriol (2008) 1.06
Characterization of bacterial spore germination using integrated phase contrast microscopy, Raman spectroscopy, and optical tweezers. Anal Chem (2010) 1.04
Characterization of spores of Bacillus subtilis that lack most coat layers. J Bacteriol (2008) 1.04
Structure-based functional studies of the effects of amino acid substitutions in GerBC, the C subunit of the Bacillus subtilis GerB spore germinant receptor. J Bacteriol (2011) 1.02
Identification of new proteins that modulate the germination of spores of bacillus species. J Bacteriol (2013) 1.02
Monitoring rates and heterogeneity of high-pressure germination of bacillus spores by phase-contrast microscopy of individual spores. Appl Environ Microbiol (2013) 1.01
Roles of small, acid-soluble spore proteins and core water content in survival of Bacillus subtilis spores exposed to environmental solar UV radiation. Appl Environ Microbiol (2009) 1.01
Structure and mechanism of action of a cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus with broad specificity phosphatase activity. J Mol Biol (2002) 1.00
Characterization of wet-heat inactivation of single spores of bacillus species by dual-trap Raman spectroscopy and elastic light scattering. Appl Environ Microbiol (2010) 0.99
Structure of the DNA-SspC complex: implications for DNA packaging, protection, and repair in bacterial spores. J Bacteriol (2004) 0.99
Effects of the SpoVT regulatory protein on the germination and germination protein levels of spores of Bacillus subtilis. J Bacteriol (2012) 0.98
Role of the Nfo (YqfS) and ExoA apurinic/apyrimidinic endonucleases in protecting Bacillus subtilis spores from DNA damage. J Bacteriol (2005) 0.98
Effects of cortex peptidoglycan structure and cortex hydrolysis on the kinetics of Ca(2+)-dipicolinic acid release during Bacillus subtilis spore germination. J Bacteriol (2011) 0.98
Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels. Arch Microbiol (2007) 0.98
Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ. J Bacteriol (2006) 0.97
Synergism between different germinant receptors in the germination of Bacillus subtilis spores. J Bacteriol (2011) 0.97
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane. J Bacteriol (2013) 0.97
YtkD and MutT protect vegetative cells but not spores of Bacillus subtilis from oxidative stress. J Bacteriol (2006) 0.97
Numbers of individual nutrient germinant receptors and other germination proteins in spores of Bacillus subtilis. J Bacteriol (2013) 0.96
Kinetics of germination of wet-heat-treated individual spores of Bacillus species, monitored by Raman spectroscopy and differential interference contrast microscopy. Appl Environ Microbiol (2011) 0.96
Effects of Mn and Fe levels on Bacillus subtilis spore resistance and effects of Mn2+, other divalent cations, orthophosphate, and dipicolinic acid on protein resistance to ionizing radiation. Appl Environ Microbiol (2010) 0.96
Differential gene expression to investigate the effect of (5Z)-4-bromo- 5-(bromomethylene)-3-butyl-2(5H)-furanone on Bacillus subtilis. Appl Environ Microbiol (2004) 0.96
Transcription of the Bacillus subtilis gerK operon, which encodes a spore germinant receptor, and comparison with that of operons encoding other germinant receptors. J Bacteriol (2006) 0.95
Roles of DacB and spm proteins in clostridium perfringens spore resistance to moist heat, chemicals, and UV radiation. Appl Environ Microbiol (2008) 0.94
Analysis of the effects of a gerP mutation on the germination of spores of Bacillus subtilis. J Bacteriol (2012) 0.94
Monitoring the kinetics of uptake of a nucleic acid dye during the germination of single spores of Bacillus species. Anal Chem (2010) 0.94
Characterization of single heat-activated Bacillus spores using laser tweezers Raman spectroscopy. Opt Express (2009) 0.93
Role of GerKB in germination and outgrowth of Clostridium perfringens spores. Appl Environ Microbiol (2009) 0.92
Germination of spores of Clostridium difficile strains, including isolates from a hospital outbreak of Clostridium difficile-associated disease (CDAD). Microbiology (2008) 0.92
Efficient inhibition of germination of coat-deficient bacterial spores by multivalent metal cations, including terbium (Tb³+). Appl Environ Microbiol (2011) 0.92
Crystal structure of the GerBC component of a Bacillus subtilis spore germinant receptor. J Mol Biol (2010) 0.92
Role of the Nfo and ExoA apurinic/apyrimidinic endonucleases in repair of DNA damage during outgrowth of Bacillus subtilis spores. J Bacteriol (2008) 0.92
Levels of glycine betaine in growing cells and spores of Bacillus species and lack of effect of glycine betaine on dormant spore resistance. J Bacteriol (2006) 0.91
PrfA protein of Bacillus species: prediction and demonstration of endonuclease activity on DNA. Protein Sci (2002) 0.91
Spore Resistance Properties. Microbiol Spectr (2014) 0.91
Effects of the binding of alpha/beta-type small, acid-soluble spore proteins on the photochemistry of DNA in spores of Bacillus subtilis and in vitro. Photochem Photobiol (2005) 0.90
Interaction of apurinic/apyrimidinic endonucleases Nfo and ExoA with the DNA integrity scanning protein DisA in the processing of oxidative DNA damage during Bacillus subtilis spore outgrowth. J Bacteriol (2013) 0.90
Workshop report: modeling the molecular mechanism of bacterial spore germination and elucidating reasons for germination heterogeneity. J Food Sci (2009) 0.90
Crystal structure of the catalytic domain of the Bacillus cereus SleB protein, important in cortex peptidoglycan degradation during spore germination. J Bacteriol (2012) 0.89
Analysis of the loss in heat and acid resistance during germination of spores of Bacillus species. J Bacteriol (2014) 0.88
High salinity alters the germination behavior of Bacillus subtilis spores with nutrient and nonnutrient germinants. Appl Environ Microbiol (2013) 0.87
Killing of spores of Bacillus subtilis by peroxynitrite appears to be caused by membrane damage. Microbiology (2002) 0.87
Multifocus confocal Raman microspectroscopy for rapid single-particle analysis. J Biomed Opt (2011) 0.87
Effects of carboxy-terminal modifications and pH on binding of a Bacillus subtilis small, acid-soluble spore protein to DNA. J Bacteriol (2003) 0.87
The synthesis and role of the mechanosensitive channel of large conductance in growth and differentiation of Bacillus subtilis. Arch Microbiol (2006) 0.86
Roles of endonuclease V, uracil-DNA glycosylase, and mismatch repair in Bacillus subtilis DNA base-deamination-induced mutagenesis. J Bacteriol (2011) 0.86
Function of the SpoVAEa and SpoVAF proteins of Bacillus subtilis spores. J Bacteriol (2014) 0.86
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination. J Bacteriol (2013) 0.86
Superdormant spores of bacillus species germinate normally with high pressure, peptidoglycan fragments, and bryostatin. J Bacteriol (2010) 0.86
Maturation of released spores is necessary for acquisition of full spore heat resistance during Bacillus subtilis sporulation. Appl Environ Microbiol (2011) 0.86