A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein delivery.

PubWeight™: 1.93‹?› | Rank: Top 3%

🔗 View Article (PMC 453153)

Published in EMBO J on December 01, 1991

Authors

P K Herman1, J H Stack, S D Emr

Author Affiliations

1: California Institute of Technology, Division of Biology, Pasadena 91125.

Articles citing this

Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae. J Cell Biol (2001) 7.68

Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway. J Cell Biol (1992) 5.50

Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p. EMBO J (1997) 5.05

Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant. Mol Biol Cell (1996) 4.71

Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast. Mol Biol Cell (1996) 4.58

A membrane-associated complex containing the Vps15 protein kinase and the Vps34 PI 3-kinase is essential for protein sorting to the yeast lysosome-like vacuole. EMBO J (1993) 3.49

Yeast vacuolar proenzymes are sorted in the late Golgi complex and transported to the vacuole via a prevacuolar endosome-like compartment. J Cell Biol (1993) 3.25

A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system. EMBO J (1995) 3.19

Golgi and vacuolar membrane proteins reach the vacuole in vps1 mutant yeast cells via the plasma membrane. J Cell Biol (1995) 2.65

Assortment of phosphatidylinositol 3-kinase complexes--Atg14p directs association of complex I to the pre-autophagosomal structure in Saccharomyces cerevisiae. Mol Biol Cell (2006) 2.63

An evolutionary proteomics approach identifies substrates of the cAMP-dependent protein kinase. Proc Natl Acad Sci U S A (2005) 2.60

Novel Golgi to vacuole delivery pathway in yeast: identification of a sorting determinant and required transport component. EMBO J (1997) 2.60

Alternative pathways for the sorting of soluble vacuolar proteins in yeast: a vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases. Mol Biol Cell (1992) 2.32

A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast. Mol Biol Cell (1997) 2.24

A sorting nexin-1 homologue, Vps5p, forms a complex with Vps17p and is required for recycling the vacuolar protein-sorting receptor. Mol Biol Cell (1997) 2.14

Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole. J Cell Biol (1998) 2.13

Synthesis and function of membrane phosphoinositides in budding yeast, Saccharomyces cerevisiae. Biochim Biophys Acta (2007) 2.08

Plant and animal pathogen recognition receptors signal through non-RD kinases. PLoS Pathog (2006) 1.87

Autophagic processes in yeast: mechanism, machinery and regulation. Genetics (2013) 1.78

AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana, is an essential protein with homology to a calcium-dependent lipid binding domain. Proc Natl Acad Sci U S A (1994) 1.72

A yeast protein related to a mammalian Ras-binding protein, Vps9p, is required for localization of vacuolar proteins. Mol Cell Biol (1996) 1.56

Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes. Science (2015) 1.21

MCD4 encodes a conserved endoplasmic reticulum membrane protein essential for glycosylphosphatidylinositol anchor synthesis in yeast. Mol Biol Cell (1999) 1.18

PtdIns 3-Kinase Orchestrates Autophagosome Formation in Yeast. J Lipids (2011) 1.11

A phosphatidylinositol 4,5-bisphosphate-sensitive casein kinase I alpha associates with synaptic vesicles and phosphorylates a subset of vesicle proteins. J Cell Biol (1995) 1.11

Genetic defects in Chediak-Higashi syndrome and the beige mouse. J Clin Immunol (1998) 1.05

Pep7p provides a novel protein that functions in vesicle-mediated transport between the yeast Golgi and endosome. Mol Biol Cell (1997) 1.05

Atg14: a key player in orchestrating autophagy. Int J Cell Biol (2011) 1.05

Structure and function of Vps15 in the endosomal G protein signaling pathway. Biochemistry (2009) 0.96

Arabidopsis AtVPS15 is essential for pollen development and germination through modulating phosphatidylinositol 3-phosphate formation. Plant Mol Biol (2011) 0.90

Genetic interaction with vps8-200 allows partial suppression of the vestigial vacuole phenotype caused by a pep5 mutation in Saccharomyces cerevisiae. Genetics (1998) 0.88

BECLIN 1-VPS34 COMPLEX ARCHITECTURE: UNDERSTANDING THE NUTS AND BOLTS OF THERAPEUTIC TARGETS. Front Biol (Beijing) (2015) 0.77

Articles cited by this

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics (1989) 105.30

Transformation of intact yeast cells treated with alkali cations. J Bacteriol (1983) 100.26

Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A (1985) 68.48

Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol (1987) 66.52

The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science (1988) 35.59

A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol (1946) 16.78

Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol (1988) 13.27

The biogenesis of lysosomes. Annu Rev Cell Biol (1989) 10.73

Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi. Annu Rev Biochem (1987) 10.25

The fungal vacuole: composition, function, and biogenesis. Microbiol Rev (1990) 6.98

The biology and enzymology of eukaryotic protein acylation. Annu Rev Biochem (1988) 6.83

Distinct sequence determinants direct intracellular sorting and modification of a yeast vacuolar protease. Cell (1987) 5.92

Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant. J Cell Biol (1991) 5.76

Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell (1986) 5.73

Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A (1986) 5.71

Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information. Mol Cell Biol (1988) 5.42

Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae. Mol Cell Biol (1990) 5.25

Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase. EMBO J (1989) 5.12

cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes. Annu Rev Biochem (1990) 5.02

Organelle assembly in yeast: characterization of yeast mutants defective in vacuolar biogenesis and protein sorting. J Cell Biol (1988) 4.90

Protein sorting in yeast: the localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide. Cell (1987) 4.76

Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase. Nature (1991) 3.86

Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J (1989) 3.79

Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family. Proc Natl Acad Sci U S A (1987) 3.42

Fatty acylation of proteins. Annu Rev Cell Biol (1988) 2.92

A novel protein kinase homolog essential for protein sorting to the yeast lysosome-like vacuole. Cell (1991) 2.64

Affinity labeling of cAMP-dependent protein kinase with p-fluorosulfonylbenzoyl adenosine. Covalent modification of lysine 71. J Biol Chem (1981) 2.48

A mammalian protein kinase with potential for serine/threonine and tyrosine phosphorylation is related to cell cycle regulators. EMBO J (1991) 2.38

Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase. Proc Natl Acad Sci U S A (1987) 2.36

Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis. Mol Cell Biol (1990) 2.32

Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle. J Cell Biol (1990) 2.25

Phosphotransferase sequence homology. Nature (1987) 2.21

Myristoyl CoA:protein N-myristoyltransferase activities from rat liver and yeast possess overlapping yet distinct peptide substrate specificities. J Biol Chem (1988) 2.17

Neither arginine nor histidine can carry out the function of lysine-295 in the ATP-binding site of p60src. Mol Cell Biol (1986) 2.15

In vivo and in vitro analysis of ptl1, a yeast ts mutant with a membrane-associated defect in protein translocation. EMBO J (1988) 1.91

Expression of the catalytic subunit of cAMP-dependent protein kinase in Escherichia coli. J Biol Chem (1989) 1.85

A new class of lysosomal/vacuolar protein sorting signals. J Biol Chem (1990) 1.38

Articles by these authors

Autophagy as a regulated pathway of cellular degradation. Science (2000) 17.27

Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol (1988) 13.27

A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast. J Cell Biol (1995) 12.18

Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I. Cell (2001) 9.08

Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. Science (1993) 7.86

The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. EMBO J (1998) 7.68

The fungal vacuole: composition, function, and biogenesis. Microbiol Rev (1990) 6.98

Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum. Cell (1994) 6.57

Suppressor mutations that restore export of a protein with a defective signal sequence. Cell (1981) 6.33

Mechanisms of protein localization. Microbiol Rev (1983) 6.12

Fab1p PtdIns(3)P 5-kinase function essential for protein sorting in the multivesicular body. Cell (1998) 6.07

Distinct sequence determinants direct intracellular sorting and modification of a yeast vacuolar protease. Cell (1987) 5.92

Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant. J Cell Biol (1991) 5.76

Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A (1986) 5.71

Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information. Mol Cell Biol (1988) 5.42

Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae. Mol Cell Biol (1990) 5.25

Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase. EMBO J (1989) 5.12

The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. Cell (1994) 5.10

Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p. EMBO J (1997) 5.05

Organelle assembly in yeast: characterization of yeast mutants defective in vacuolar biogenesis and protein sorting. J Cell Biol (1988) 4.90

Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant. Mol Biol Cell (1996) 4.71

Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast. Mol Biol Cell (1996) 4.58

A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol (1998) 4.41

Mammalian tumor susceptibility gene 101 (TSG101) and the yeast homologue, Vps23p, both function in late endosomal trafficking. Traffic (2000) 4.17

A multispecificity syntaxin homologue, Vam3p, essential for autophagic and biosynthetic protein transport to the vacuole. J Cell Biol (1997) 4.16

Phosphoinositides as regulators in membrane traffic. Science (1996) 4.13

The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal. J Cell Biol (1986) 4.07

Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Mol Cell (1998) 4.01

An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast. Proc Natl Acad Sci U S A (1983) 3.95

Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product. Mol Cell Biol (1988) 3.74

New component of the vacuolar class C-Vps complex couples nucleotide exchange on the Ypt7 GTPase to SNARE-dependent docking and fusion. J Cell Biol (2000) 3.69

Mutations affecting localization of an Escherichia coli outer membrane protein, the bacteriophage lambda receptor. J Mol Biol (1980) 3.62

Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products. J Cell Biol (1997) 3.61

The role of phosphoinositides in membrane transport. Curr Opin Cell Biol (2001) 3.57

The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex. J Cell Biol (1989) 3.55

A membrane-associated complex containing the Vps15 protein kinase and the Vps34 PI 3-kinase is essential for protein sorting to the yeast lysosome-like vacuole. EMBO J (1993) 3.49

Sequence analysis of mutations that prevent export of lambda receptor, an Escherichia coli outer membrane protein. Nature (1980) 3.36

Signal-mediated retrieval of a membrane protein from the Golgi to the ER in yeast. J Cell Biol (1994) 3.32

The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole. Cell (1997) 3.30

Class C Vps protein complex regulates vacuolar SNARE pairing and is required for vesicle docking/fusion. Mol Cell (2000) 3.30

A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole. Mol Biol Cell (1997) 3.27

Yeast vacuolar proenzymes are sorted in the late Golgi complex and transported to the vacuole via a prevacuolar endosome-like compartment. J Cell Biol (1993) 3.25

Fab1p is essential for PtdIns(3)P 5-kinase activity and the maintenance of vacuolar size and membrane homeostasis. J Cell Biol (1998) 3.24

Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J Cell Biol (1995) 3.16

Phox domain interaction with PtdIns(3)P targets the Vam7 t-SNARE to vacuole membranes. Nat Cell Biol (2001) 3.14

Intracellular targeting and import of an F1-ATPase beta-subunit-beta-galactosidase hybrid protein into yeast mitochondria. Proc Natl Acad Sci U S A (1984) 3.02

Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins. Annu Rev Cell Dev Biol (1995) 2.93

A mechanism of protein localization: the signal hypothesis and bacteria. J Cell Biol (1980) 2.91

VPS21 encodes a rab5-like GTP binding protein that is required for the sorting of yeast vacuolar proteins. EMBO J (1994) 2.90

Distinct roles for the yeast phosphatidylinositol 4-kinases, Stt4p and Pik1p, in secretion, cell growth, and organelle membrane dynamics. Mol Biol Cell (2000) 2.87

Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations. J Biol Chem (1982) 2.85

A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery. Cell (1982) 2.84

Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking. Mol Cell Biol (1998) 2.80

Cytoplasm to vacuole trafficking of aminopeptidase I requires a t-SNARE-Sec1p complex composed of Tlg2p and Vps45p. EMBO J (1999) 2.77

Role for phosphatidylinositol 3-kinase in the sorting and transport of newly synthesized lysosomal enzymes in mammalian cells. J Cell Biol (1995) 2.74

Phosphoinositide signaling and the regulation of membrane trafficking in yeast. Trends Biochem Sci (2000) 2.72

Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. J Biol Chem (1994) 2.66

Novel PI(4)P 5-kinase homologue, Fab1p, essential for normal vacuole function and morphology in yeast. Mol Biol Cell (1995) 2.64

A novel protein kinase homolog essential for protein sorting to the yeast lysosome-like vacuole. Cell (1991) 2.64

A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15. J Cell Biol (1996) 2.62

Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles. J Cell Sci (1994) 2.61

Novel Golgi to vacuole delivery pathway in yeast: identification of a sorting determinant and required transport component. EMBO J (1997) 2.60

The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast. J Biol Chem (1993) 2.50

The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization. Mol Biol Cell (1995) 2.50

Phosphoinositide signaling and turnover: PtdIns(3)P, a regulator of membrane traffic, is transported to the vacuole and degraded by a process that requires lumenal vacuolar hydrolase activities. EMBO J (1998) 2.44

Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis. J Cell Biol (1998) 2.43

A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation. Mol Cell Biol (1991) 2.38

Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein. Proc Natl Acad Sci U S A (1978) 2.37

Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis. Mol Cell Biol (1990) 2.32

ARF is required for maintenance of yeast Golgi and endosome structure and function. Mol Biol Cell (1998) 2.32

Alternative pathways for the sorting of soluble vacuolar proteins in yeast: a vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases. Mol Biol Cell (1992) 2.32

GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae. J Bacteriol (1986) 2.30

A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast. Mol Biol Cell (1997) 2.24

Mutants of Saccharomyces cerevisiae that block intervacuole vesicular traffic and vacuole division and segregation. Proc Natl Acad Sci U S A (1990) 2.18

Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis. EMBO J (1999) 2.18

COPI-independent anterograde transport: cargo-selective ER to Golgi protein transport in yeast COPI mutants. J Cell Biol (1997) 2.16

Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. Curr Biol (1999) 2.15

A sorting nexin-1 homologue, Vps5p, forms a complex with Vps17p and is required for recycling the vacuolar protein-sorting receptor. Mol Biol Cell (1997) 2.14

Protein traffic in the yeast endocytic and vacuolar protein sorting pathways. Curr Opin Cell Biol (1998) 2.13

Location, location, location: membrane targeting directed by PX domains. Science (2001) 2.09

Sac1 lipid phosphatase and Stt4 phosphatidylinositol 4-kinase regulate a pool of phosphatidylinositol 4-phosphate that functions in the control of the actin cytoskeleton and vacuole morphology. Mol Biol Cell (2001) 1.99

Importance of secondary structure in the signal sequence for protein secretion. Proc Natl Acad Sci U S A (1983) 1.98

The AP-3 complex: a coat of many colours. Trends Cell Biol (1998) 1.92

Invertase beta-galactosidase hybrid proteins fail to be transported from the endoplasmic reticulum in Saccharomyces cerevisiae. Mol Cell Biol (1984) 1.91

D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. J Bacteriol (1984) 1.89

In vitro reconstitution of intercompartmental protein transport to the yeast vacuole. J Cell Biol (1990) 1.85

Identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery. Protein Sci (1999) 1.84

Phosphoinositide 3-kinases and their FYVE domain-containing effectors as regulators of vacuolar/lysosomal membrane trafficking pathways. J Biol Chem (1999) 1.80

Clathrin-dependent localization of alpha 1,3 mannosyltransferase to the Golgi complex of Saccharomyces cerevisiae. J Cell Biol (1994) 1.79

Phosphatidylinositol 3-phosphate recognition by the FYVE domain. Mol Cell (1999) 1.76

The class C Vps complex functions at multiple stages of the vacuolar transport pathway. Traffic (2001) 1.73

AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana, is an essential protein with homology to a calcium-dependent lipid binding domain. Proc Natl Acad Sci U S A (1994) 1.72

Yeast Gga coat proteins function with clathrin in Golgi to endosome transport. Mol Biol Cell (2001) 1.72

Formation of AP-3 transport intermediates requires Vps41 function. Nat Cell Biol (1999) 1.69

Molecular components of the signal sequence that function in the initiation of protein export. J Cell Biol (1982) 1.65

A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development. Mol Cell Biol (1995) 1.64

Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway. EMBO J (1993) 1.59

Acidic di-leucine motif essential for AP-3-dependent sorting and restriction of the functional specificity of the Vam3p vacuolar t-SNARE. J Cell Biol (1998) 1.58