Published in Trends Biotechnol on January 13, 2015
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat Biotechnol (2015) 1.71
Off-target Effects in CRISPR/Cas9-mediated Genome Engineering. Mol Ther Nucleic Acids (2015) 1.23
Evaluation of TCR Gene Editing Achieved by TALENs, CRISPR/Cas9, and megaTAL Nucleases. Mol Ther (2015) 1.04
Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery. Nat Methods (2015) 0.96
Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions. Cell Stem Cell (2016) 0.87
Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations. Nucleic Acids Res (2016) 0.86
Patterns of CRISPR/Cas9 activity in plants, animals and microbes. Plant Biotechnol J (2016) 0.85
Reprogramming and transdifferentiation for cardiovascular development and regenerative medicine: where do we stand? EMBO Mol Med (2015) 0.85
Long-term multilineage engraftment of autologous genome-edited hematopoietic stem cells in nonhuman primates. Blood (2016) 0.84
CRISPR guide RNA design for research applications. FEBS J (2016) 0.83
Towards a new era in medicine: therapeutic genome editing. Genome Biol (2015) 0.83
Origins of Programmable Nucleases for Genome Engineering. J Mol Biol (2015) 0.83
Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing. Nat Protoc (2016) 0.83
Loss-of-function genetic tools for animal models: cross-species and cross-platform differences. Nat Rev Genet (2016) 0.82
PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing. Nat Commun (2015) 0.82
Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo. Nucleic Acids Res (2016) 0.80
An Overview of CRISPR-Based Tools and Their Improvements: New Opportunities in Understanding Plant-Pathogen Interactions for Better Crop Protection. Front Plant Sci (2016) 0.80
DNA-free genome editing methods for targeted crop improvement. Plant Cell Rep (2016) 0.80
Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineering. Mol Ther Nucleic Acids (2015) 0.80
Strategies for precision modulation of gene expression by epigenome editing: an overview. Epigenetics Chromatin (2015) 0.79
A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells. PLoS One (2016) 0.78
Multidimensional Genome-wide Analyses Show Accurate FVIII Integration by ZFN in Primary Human Cells. Mol Ther (2015) 0.77
To CRISPR and beyond: the evolution of genome editing in stem cells. Regen Med (2016) 0.75
Engineered Swine Models of Cancer. Front Genet (2016) 0.75
Weaknesses and Pitfalls of Using Mice and Rats in Cancer Chemoprevention Studies. J Cancer (2015) 0.75
DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins. Front Plant Sci (2016) 0.75
Adenoviral vectors encoding CRISPR/Cas9 multiplexes rescue dystrophin synthesis in unselected populations of DMD muscle cells. Sci Rep (2016) 0.75
Dual loss of human POLQ and LIG4 abolishes random integration. Nat Commun (2017) 0.75
Technical Considerations for the Use of CRISPR/Cas9 in Hematology Research. Exp Hematol (2017) 0.75
A Critical Analysis of the Role of SNARE Protein SEC22B in Antigen Cross-Presentation. Cell Rep (2017) 0.75
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol (2013) 19.99
Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature (2005) 19.15
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol (2013) 18.01
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat Biotechnol (2008) 14.75
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell (2013) 13.37
Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol (2011) 11.76
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol (2013) 11.58
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol (2013) 11.35
TAL effectors: customizable proteins for DNA targeting. Science (2011) 9.47
Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics (2002) 9.09
Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly. Genome Res (2009) 8.69
Knockout rats generated by embryo microinjection of TALENs. Nat Biotechnol (2011) 8.45
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol (2013) 8.40
Heritable gene targeting in zebrafish using customized TALENs. Nat Biotechnol (2011) 8.34
Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection. Nat Methods (2011) 6.90
Cas9 as a versatile tool for engineering biology. Nat Methods (2013) 6.87
A rapid and general assay for monitoring endogenous gene modification. Methods Mol Biol (2010) 6.77
An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nat Biotechnol (2011) 6.44
Gene targeting using zinc finger nucleases. Nat Biotechnol (2005) 6.24
Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases. Proc Natl Acad Sci U S A (2007) 5.93
Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells. Proc Natl Acad Sci U S A (1994) 5.37
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat Biotechnol (2014) 5.07
A TALEN genome-editing system for generating human stem cell-based disease models. Cell Stem Cell (2012) 4.49
Simple methods for generating and detecting locus-specific mutations induced with TALENs in the zebrafish genome. PLoS Genet (2012) 4.42
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol (2014) 4.41
Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains. Nucleic Acids Res (2000) 4.34
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nat Biotechnol (2014) 4.16
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity. Nucleic Acids Res (2013) 4.11
Tracking genome engineering outcome at individual DNA breakpoints. Nat Methods (2011) 4.05
Mutation detection using Surveyor nuclease. Biotechniques (2004) 3.72
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat Biotechnol (2014) 3.72
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat Biotechnol (2014) 3.66
Surrogate reporters for enrichment of cells with nuclease-induced mutations. Nat Methods (2011) 3.22
Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing. Cell Stem Cell (2014) 3.16
Genome engineering with targetable nucleases. Annu Rev Biochem (2014) 3.14
Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility. Nucleic Acids Res (2001) 3.04
Comparison of zinc finger nucleases for use in gene targeting in mammalian cells. Mol Ther (2008) 2.87
Targeted chromosomal duplications and inversions in the human genome using zinc finger nucleases. Genome Res (2011) 2.85
Optimization of scarless human stem cell genome editing. Nucleic Acids Res (2013) 2.78
An erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin level. Science (2013) 2.71
Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases. J Mol Biol (2010) 2.65
In situ genetic correction of the sickle cell anemia mutation in human induced pluripotent stem cells using engineered zinc finger nucleases. Stem Cells (2011) 2.52
Attenuation of zinc finger nuclease toxicity by small-molecule regulation of protein levels. PLoS Genet (2009) 2.18
Targeted gene knockout by direct delivery of zinc-finger nuclease proteins. Nat Methods (2012) 2.07
Repair of strand breaks by homologous recombination. Cold Spring Harb Perspect Biol (2013) 1.96
Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases. Nat Commun (2012) 1.90
Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy. Curr Gene Ther (2011) 1.76
Gene-editing nucleases. Nat Methods (2012) 1.66
Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity. Nat Methods (2014) 1.66
TALENs and ZFNs are associated with different mutation signatures. Nat Methods (2013) 1.66
Reading frame correction by targeted genome editing restores dystrophin expression in cells from Duchenne muscular dystrophy patients. Mol Ther (2013) 1.63
Increasing frequencies of site-specific mutagenesis and gene targeting in Arabidopsis by manipulating DNA repair pathways. Genome Res (2013) 1.57
Frequent endonuclease cleavage at off-target locations in vivo. Mol Ther (2010) 1.52
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Cell Rep (2014) 1.50
In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites. Nucleic Acids Res (2013) 1.46
An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage. Nucleic Acids Res (2013) 1.45
Engineering human tumour-associated chromosomal translocations with the RNA-guided CRISPR-Cas9 system. Nat Commun (2014) 1.43
A large-scale in vivo analysis reveals that TALENs are significantly more mutagenic than ZFNs generated using context-dependent assembly. Nucleic Acids Res (2013) 1.40
megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering. Nucleic Acids Res (2013) 1.30
Coupling endonucleases with DNA end-processing enzymes to drive gene disruption. Nat Methods (2012) 1.28
Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair. Proc Natl Acad Sci U S A (2014) 1.21
A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture. Nucleic Acids Res (2015) 1.20
Gene editing: not just for translation anymore. Nat Methods (2011) 1.16
TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity. Nucleic Acids Res (2014) 1.16
SAPTA: a new design tool for improving TALE nuclease activity. Nucleic Acids Res (2014) 0.96
Lentiviral and targeted cellular barcoding reveals ongoing clonal dynamics of cell lines in vitro and in vivo. Genome Biol (2014) 0.92
Targeted gene therapies: tools, applications, optimization. Crit Rev Biochem Mol Biol (2012) 0.91
A streamlined method for detecting structural variants in cancer genomes by short read paired-end sequencing. PLoS One (2012) 0.85
Codon swapping of zinc finger nucleases confers expression in primary cells and in vivo from a single lentiviral vector. Curr Gene Ther (2014) 0.78
Seeing the light: integrating genome engineering with double-strand break repair. Nat Methods (2011) 0.78
An unbiased method for detection of genome-wide off-target effects in cell lines treated with zinc finger nucleases. Methods Mol Biol (2014) 0.76
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Cell Rep (2014) 1.50
An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage. Nucleic Acids Res (2013) 1.45
TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity. Nucleic Acids Res (2014) 1.16
Shedding light on the dynamics of endocytosis and viral budding. Proc Natl Acad Sci U S A (2005) 0.99
Molecular Biomechanics: The Molecular Basis of How Forces Regulate Cellular Function. Mol Cell Biomech (2010) 0.91
CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors. Cell Rep (2017) 0.75