Binding of a soluble factor of Escherichia coli to preproteins does not require ATP and appears to be the first step in protein export.

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Published in Proc Natl Acad Sci U S A on April 01, 1989

Authors

M Watanabe1, G Blobel

Author Affiliations

1: Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021.

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Signal recognition particle: a ribonucleoprotein required for cotranslational translocation of proteins, isolation and properties. Methods Enzymol (1983) 2.87

Studies on free and membrane-bound ribosomes in rat liver. II. Interaction of ribosomes and membranes. J Mol Biol (1967) 2.85

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Nucleotide sequence of the yeast nuclear gene for cytochrome c peroxidase precursor. Functional implications of the pre sequence for protein transport into mitochondria. J Biol Chem (1982) 2.78

Functional interaction of plant ribosomes with animal microsomal membranes. Biochem Biophys Res Commun (1977) 2.77

A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96. J Cell Biol (1999) 2.76

Nuclear lamina and the structural organization of the nuclear envelope. Cold Spring Harb Symp Quant Biol (1982) 2.73

Purification of microsomal signal peptidase as a complex. Proc Natl Acad Sci U S A (1986) 2.67

Transfer of proteins across membranes, Biosynthesis in vitro of pretrypsinogen and trypsinogen by cell fractions of canine pancreas. Eur J Biochem (1978) 2.62

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