Published in Proc Natl Acad Sci U S A on September 01, 1978
Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch. Nucleic Acids Res (1979) 14.12
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. Nucleic Acids Res (1982) 13.52
Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence. Proc Natl Acad Sci U S A (1980) 10.27
Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments. Nucleic Acids Res (1982) 5.72
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function. Proc Natl Acad Sci U S A (1982) 4.64
Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA. Nucleic Acids Res (1981) 3.87
Carboxy terminus of polyoma middle-sized tumor antigen is required for attachment to membranes, associated protein kinase activities, and cell transformation. Proc Natl Acad Sci U S A (1982) 3.12
Restriction enzyme digestion of hemimethylated DNA. Nucleic Acids Res (1981) 2.87
An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res (1983) 2.69
Clonal inheritance of the pattern of DNA methylation in mouse cells. Proc Natl Acad Sci U S A (1982) 2.52
Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C. Proc Natl Acad Sci U S A (1981) 2.09
Studies on the biological role of dna methylation; IV. Mode of methylation of DNA in E. coli cells. Nucleic Acids Res (1980) 2.04
Defined transversion mutations at a specific position in DNA using synthetic oligodeoxyribonucleotides as mutagens. Nucleic Acids Res (1979) 1.99
Biological activities of EGF-receptor mutants with individually altered autophosphorylation sites. EMBO J (1988) 1.92
An amber suppressor tRNA gene derived by site-specific mutagenesis: cloning and function in mammalian cells. Proc Natl Acad Sci U S A (1982) 1.86
Site-directed mutagenesis reveals a liver transcription factor essential for the albumin transcriptional enhancer. Proc Natl Acad Sci U S A (1990) 1.77
Insertion mutant of bacteriophage f1 sensitive to EcoRI. Proc Natl Acad Sci U S A (1979) 1.76
Thiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue. Proc Natl Acad Sci U S A (1982) 1.74
Expression of a X. laevis tRNATyr gene in mammalian cells. Nucleic Acids Res (1982) 1.66
In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency. Nucleic Acids Res (1983) 1.59
Construction of a specific amber codon in the simian virus 40 T-antigen gene by site-directed mutagenesis. J Virol (1980) 1.56
Bacteriorhodopsin: partial sequence of mRNA provides amino acid sequence in the precursor region. Proc Natl Acad Sci U S A (1981) 1.48
Control of adenovirus E1B mRNA synthesis by a shift in the activities of RNA splice sites. Mol Cell Biol (1984) 1.31
Oligonucleotide-directed mutagenesis of gene IX of bacteriophage M13. Nucleic Acids Res (1982) 1.29
Contact points between transcription machinery and the fibroin gene promoter deduced by functional tests of single-base substitution mutants. Proc Natl Acad Sci U S A (1984) 1.21
In vitro characterization of the fibroin gene promoter by the use of single-base substitution mutants. Proc Natl Acad Sci U S A (1982) 1.21
Adenovirus terminal protein protects single stranded DNA from digestion by a cellular exonuclease. Nucleic Acids Res (1980) 1.20
Site specific mutagenesis: insertion of single noncomplementary nucleotides at specified sites by error-directed DNA polymerization. Nucleic Acids Res (1984) 1.13
A method for introducing random single point deletions in specific DNA target sequences using oligonucleotides. Nucleic Acids Res (1989) 1.05
Construction, stable transformation, and function of an amber suppressor tRNA gene in Drosophila melanogaster. Proc Natl Acad Sci U S A (1989) 1.04
Synthetic DNA and medicine. Am J Hum Genet (1979) 1.00
Elimination of mRNA splicing by a point mutation outside the conserved GU at 5' splice sites. Nucleic Acids Res (1984) 0.99
Enzymatic techniques for the isolation of random single-base substitutions in vitro at high frequency. Proc Natl Acad Sci U S A (1984) 0.97
DNA pairing is an important step in the process of targeted nucleotide exchange. Nucleic Acids Res (2003) 0.95
Control of oligomeric enzyme thermostability by protein engineering. Proc Natl Acad Sci U S A (1987) 0.94
Synthesis and properties of oligodeoxynucleotides with an AP site at a preselected position. Nucleic Acids Res (1987) 0.92
Introduction of restriction enzyme sites in protein-coding DNA sequences by site-specific mutagenesis not affecting the amino acid sequence: a computer program. Nucleic Acids Res (1984) 0.84
Nucleotide sequence of bacteriophage phi X174 DNA. Nature (1977) 95.69
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science (1977) 9.28
Mutagenesis at a specific position in a DNA sequence. J Biol Chem (1978) 7.56
Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta (1963) 7.28
Synthetic lac operator DNA is functional in vivo. Nature (1976) 5.36
Overlapping genes in bacteriophage phiX174. Nature (1976) 4.35
Genetic assay for small fragments of bacteriophage phi X174 deoxyribonucleic acid. J Virol (1971) 3.78
Structure of the DNA of bacteriophage phiX174. VII. Methylation. J Mol Biol (1970) 3.23
Enzymatic synthesis of DNA, XXIV. Synthesis of infectious phage phi-X174 DNA. Proc Natl Acad Sci U S A (1967) 3.09
Bological activity of phi X174 replicative form DNA fragments. Biochim Biophys Acta (1970) 2.44
PhiX174 replicative form DNA replication, origin and direction. J Mol Biol (1972) 2.16
On the origin of bacteriophage phiX174 replicative form DNA replication. Virology (1976) 1.09
Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch. Nucleic Acids Res (1979) 14.12
DNA methylation and gene function. Science (1980) 14.01
Lac repressor-operator interaction. I. Equilibrium studies. J Mol Biol (1970) 13.32
LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development. Cell (2001) 11.99
The lac repressor-operator interaction. 3. Kinetic studies. J Mol Biol (1970) 10.07
The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA. Nucleic Acids Res (1981) 9.56
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science (1977) 9.28
X inactivation, differentiation, and DNA methylation. Cytogenet Cell Genet (1975) 8.74
On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol (1968) 8.34
Sequence specificity of methylation in higher plant DNA. Nature (1981) 7.58
Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides. Proc Natl Acad Sci U S A (1983) 7.52
Genomic sequencing and methylation analysis by ligation mediated PCR. Science (1989) 7.38
Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin. Proc Natl Acad Sci U S A (1981) 7.24
Crystal structure analysis of a complete turn of B-DNA. Nature (1980) 7.18
Directed deletion of a yeast transfer RNA intervening sequence. Science (1980) 6.67
Primary structure of the receptor for calcium channel blockers from skeletal muscle. Nature (1987) 6.66
Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support. Nucleic Acids Res (1982) 6.38
Structure of a B-DNA dodecamer: conformation and dynamics. Proc Natl Acad Sci U S A (1981) 6.38
Chemical synthesis of genes for human insulin. Proc Natl Acad Sci U S A (1978) 6.09
Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor. Nature (1989) 6.07
Lac repressor binding to non-operator DNA: detailed studies and a comparison of eequilibrium and rate competition methods. J Mol Biol (1972) 5.62
Sp1 elements protect a CpG island from de novo methylation. Nature (1994) 5.60
High-salt d(CpGpCpG), a left-handed Z' DNA double helix. Nature (1980) 5.46
Synthetic lac operator DNA is functional in vivo. Nature (1976) 5.36
Maternal-specific methylation of the imprinted mouse Igf2r locus identifies the expressed locus as carrying the imprinting signal. Cell (1993) 5.22
A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322. Gene (1981) 5.06
DNA binding of the lac repressor. J Mol Biol (1968) 5.02
Mammalian X-chromosome inactivation. Annu Rev Genet (1983) 4.89
Chemical synthesis of restriction enzyme recognition sites useful for cloning. Science (1977) 4.81
Expression in Escherichia coli of chemically synthesized genes for human insulin. Proc Natl Acad Sci U S A (1979) 4.72
Structural homology of Torpedo californica acetylcholine receptor subunits. Nature (1983) 4.70
Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method. Nucleic Acids Res (1980) 4.69
Simplications in the synthesis of short oligonucleotide blocks. Nucleic Acids Res (1980) 4.64
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function. Proc Natl Acad Sci U S A (1982) 4.64
Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel. Nature (1989) 4.55
Synthesis and use of synthetic oligonucleotides. Annu Rev Biochem (1984) 4.53
Purification and DNA-binding properties of the catabolite gene activator protein. Proc Natl Acad Sci U S A (1971) 4.43
alpha 1-antitrypsin deficiency detection by direct analysis of the mutation in the gene. Nature (1983) 4.38
Clonal analysis using recombinant DNA probes from the X-chromosome. Cancer Res (1987) 4.25
Improved triester approach for the synthesis of pentadecathymidylic acid. J Am Chem Soc (1975) 4.08
Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II. Science (1979) 4.06
Developmental pattern of gene-specific DNA methylation in the mouse embryo and germ line. Genes Dev (1992) 4.02
Direct detection of methylated cytosine in DNA by use of the restriction enzyme MspI. Nucleic Acids Res (1979) 3.99
Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA. Nucleic Acids Res (1981) 3.87
In vitro and in vivo activity of NY-198, a new difluorinated quinolone. Antimicrob Agents Chemother (1987) 3.84
Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature (1979) 3.80
Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation. Mol Cell Biol (1990) 3.78
Structure, dynamics, and energetics of deoxyguanosine . thymidine wobble base pair formation in the self-complementary d(CGTGAATTCGCG) duplex in solution. Biochemistry (1982) 3.76
The general affinity of lac repressor for E. coli DNA: implications for gene regulation in procaryotes and eucaryotes. Cell (1975) 3.73
Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence. Nature (1982) 3.69
Sequence of the promoter region of the gene for human X-linked 3-phosphoglycerate kinase. Gene (1984) 3.67
Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method. Nucleic Acids Res (1980) 3.59
Ancestral, mammalian-wide subfamilies of LINE-1 repetitive sequences. J Mol Biol (1995) 3.50
Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane. Proc Natl Acad Sci U S A (1982) 3.44
Ly-C: a third locus specifying alloantigens expressed only on thymocytes and lymphocytes. Transplantation (1971) 3.42
Substrate and sequence specificity of a eukaryotic DNA methylase. Nature (1982) 3.42
Methylation of CpG sequences in eukaryotic DNA. FEBS Lett (1981) 3.39
Genetic linkage relationships of loci specifying differentiation alloantigens in the mouse. Transplantation (1972) 3.34
Premelting and melting transitions in the d(CGCGAATTCGCG) self-complementary duplex in solution. Biochemistry (1982) 3.30
5-methylcytosine, gene regulation, and cancer. Adv Cancer Res (1983) 3.27
Structure of the DNA of bacteriophage phiX174. VII. Methylation. J Mol Biol (1970) 3.23
Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method. Nucleic Acids Res (1980) 3.20
The use of arylsulfonyltriazoles for the synthesis of oligonucleotides by the triester approach. J Am Chem Soc (1975) 3.16
Cloning and sequence analysis of cDNA for porcine beta-neo-endorphin/dynorphin precursor. Nature (1982) 3.09
Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs. Nucleic Acids Res (1987) 3.05
An atypical PKC directly associates and colocalizes at the epithelial tight junction with ASIP, a mammalian homologue of Caenorhabditis elegans polarity protein PAR-3. J Cell Biol (1998) 3.04
Pattern of methylation of two genes coding for housekeeping functions. Proc Natl Acad Sci U S A (1983) 3.03
Extra adenosine stacks into the self-complementary d(CGCAGAATTCGCG) duplex in solution. Biochemistry (1982) 3.02
Methylation of foreign DNA sequences in eukaryotic cells. Proc Natl Acad Sci U S A (1980) 3.01
Malignant peripheral nerve sheath tumor: analysis of treatment outcome. Int J Radiat Oncol Biol Phys (1998) 2.99
On the assay, isolation and characterization of the lac repressor. J Mol Biol (1968) 2.94
In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells. Proc Natl Acad Sci U S A (1982) 2.92
Tiggers and DNA transposon fossils in the human genome. Proc Natl Acad Sci U S A (1996) 2.89
Restriction enzyme digestion of hemimethylated DNA. Nucleic Acids Res (1981) 2.87
Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M.SssI). Nucleic Acids Res (1990) 2.86
A quantitative HpaII-PCR assay to measure methylation of DNA from a small number of cells. Nucleic Acids Res (1990) 2.81
Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin. Nature (1982) 2.79
Chemical synthesis and sequence studies of deoxyribooligonucleotides which constitute the duplex sequence of the lactose operator of Escherichia coli. J Biol Chem (1975) 2.79
Cloning and sequence analysis of calf cDNA and human genomic DNA encoding alpha-subunit precursor of muscle acetylcholine receptor. Nature (1983) 2.78
In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human PGK-1. Genes Dev (1990) 2.71
Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli. J Biol Chem (1983) 2.66
The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage. J Mol Biol (1969) 2.59
The absence of detectable methylated bases in Drosophila melanogaster DNA. FEBS Lett (1982) 2.57
Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences. Nature (1983) 2.55
DNA methylation and its possible biological roles. Prog Nucleic Acid Res Mol Biol (1981) 2.55
Clonal inheritance of the pattern of DNA methylation in mouse cells. Proc Natl Acad Sci U S A (1982) 2.52
Polymerase chain reaction-aided genomic sequencing of an X chromosome-linked CpG island: methylation patterns suggest clonal inheritance, CpG site autonomy, and an explanation of activity state stability. Proc Natl Acad Sci U S A (1990) 2.51
Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures. J Cell Biol (2001) 2.51