Published in Cell on June 01, 1980
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev (1983) 24.58
A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics (1987) 14.69
Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1981) 9.45
Sequence of the lacZ gene of Escherichia coli. EMBO J (1983) 6.46
Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol (1984) 6.23
Mechanism of action of the lexA gene product. Proc Natl Acad Sci U S A (1981) 6.13
Uses of lac fusions for the study of biological problems. Microbiol Rev (1985) 4.38
Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli. Proc Natl Acad Sci U S A (1983) 4.22
NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae. Mol Cell Biol (1995) 3.82
Enhanced expression of cro-beta-galactosidase fusion proteins under the control of the PR promoter of bacteriophage lambda. EMBO J (1982) 3.69
Amino acid sequences that determine the nuclear localization of yeast histone 2B. Mol Cell Biol (1987) 3.31
Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti plasmid pTiA6 encodes an enzyme that catalyzes synthesis of indoleacetic acid. Proc Natl Acad Sci U S A (1984) 3.21
A DNA fragment with an alpha-phosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo. Proc Natl Acad Sci U S A (1981) 3.14
Determination of the optimal aligned spacing between the Shine-Dalgarno sequence and the translation initiation codon of Escherichia coli mRNAs. Nucleic Acids Res (1994) 3.08
In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage. Proc Natl Acad Sci U S A (1984) 3.06
Tripartite upstream promoter element essential for expression of Saccharomyces cerevisiae ribosomal protein genes. Mol Cell Biol (1986) 3.00
A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus. EMBO J (1984) 2.96
Site-specific methylases induce the SOS DNA repair response in Escherichia coli. J Bacteriol (1987) 2.94
A sequence from Drosophila melanogaster 18S rRNA bearing the conserved hypermodified nucleoside am psi: analysis by reverse transcription and high-performance liquid chromatography. Nucleic Acids Res (1981) 2.69
The yeast RAD50 gene encodes a predicted 153-kD protein containing a purine nucleotide-binding domain and two large heptad-repeat regions. Genetics (1989) 2.63
Regulation at the glnL-operator-promoter of the complex glnALG operon of Escherichia coli. J Bacteriol (1983) 2.52
Open reading frame cloning: identification, cloning, and expression of open reading frame DNA. Proc Natl Acad Sci U S A (1982) 2.39
Specificity of mismatch repair following transformation of Saccharomyces cerevisiae with heteroduplex plasmid DNA. Proc Natl Acad Sci U S A (1989) 2.33
Trp aporepressor production is controlled by autogenous regulation and inefficient translation. Proc Natl Acad Sci U S A (1982) 2.30
Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K. Proc Natl Acad Sci U S A (1983) 2.14
Identification of a sequence responsible for periodic synthesis of yeast histone 2A mRNA. Proc Natl Acad Sci U S A (1982) 2.07
Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis. Proc Natl Acad Sci U S A (1984) 1.97
Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC). Nucleic Acids Res (1981) 1.95
Expression of the human fibroblast interferon gene in Escherichia coli. Proc Natl Acad Sci U S A (1980) 1.84
In vitro construction and characterization of phoA-lacZ gene fusions in Escherichia coli. J Bacteriol (1983) 1.82
Bacteria mature preproinsulin to proinsulin. Proc Natl Acad Sci U S A (1980) 1.75
Formation of supercoiling domains in plasmid pBR322. J Bacteriol (1989) 1.62
IncFII plasmid incompatibility product and its target are both RNA transcripts. J Bacteriol (1984) 1.61
Highlights of the DNA cutters: a short history of the restriction enzymes. Nucleic Acids Res (2013) 1.60
Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I). Nucleic Acids Res (1985) 1.56
A new type of fusion analysis applicable to many organisms: protein fusions to the URA3 gene of yeast. Genetics (1987) 1.42
Mutant lambda phage repressor with a specific defect in its positive control function. Proc Natl Acad Sci U S A (1982) 1.39
Construction of a general vector for efficient expression of mammalian proteins in bacteria: use of a synthetic ribosome binding site. Proc Natl Acad Sci U S A (1981) 1.31
Isolation and partial characterization of a monoclonal antibody to the Rous sarcoma virus transforming protein pp60src. J Virol (1983) 1.31
Expression of polyoma early gene products in E. coli. Nucleic Acids Res (1985) 1.29
The incompatibility product of IncFII R plasmid NR1 controls gene expression in the plasmid replication region. J Bacteriol (1982) 1.28
Posttranscriptional regulation and assembly into ribosomes of a Saccharomyces cerevisiae ribosomal protein-beta-galactosidase fusion. Mol Cell Biol (1985) 1.11
Improved genetic selection for screening bacteriophage libraries by homologous recombination in vivo. Proc Natl Acad Sci U S A (1990) 1.09
UP element-dependent transcription at the Escherichia coli rrnB P1 promoter: positional requirements and role of the RNA polymerase alpha subunit linker. Nucleic Acids Res (2001) 1.08
Physiological implications of the substrate specificities of acetohydroxy acid synthases from varied organisms. J Bacteriol (1990) 1.07
Regulation of transcription of the repA1 gene in the replication control region of IncFII plasmid NR1 by gene dosage of the repA2 transcription repressor protein. J Bacteriol (1985) 1.07
Control of yeast gene expression by transposable elements: maximum expression requires a functional Ty activator sequence and a defective Ty promoter. Mol Cell Biol (1988) 1.06
Initiation factor-independent translation of mRNAs from Gram-positive bacteria. Proc Natl Acad Sci U S A (1981) 1.00
High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector. Proc Natl Acad Sci U S A (1984) 1.00
Analysis of the individual regulatory components of the IncFII plasmid replication control system. J Bacteriol (1985) 0.99
Stabilization of proteins by a bacteriophage T4 gene cloned in Escherichia coli. Proc Natl Acad Sci U S A (1983) 0.99
Relationship between structure of the 5' noncoding region of viral mRNA and efficiency in the initiation step of protein synthesis in a eukaryotic system. Proc Natl Acad Sci U S A (1982) 0.96
Wild-type bacteriophage T4 is restricted by the lambda rex genes. J Virol (1987) 0.95
Expression of the polyomavirus minor capsid proteins VP2 and VP3 in Escherichia coli: in vitro interactions with recombinant VP1 capsomeres. J Virol (1995) 0.95
The UP element is necessary but not sufficient for growth rate-dependent control of the Escherichia coli guaB promoter. J Bacteriol (2008) 0.89
Immunomagnetic separation and solid-phase detection of Bordetella pertussis. J Clin Microbiol (1996) 0.88
Gene II of phage f1: its functions and its products. Proc Natl Acad Sci U S A (1981) 0.87
Increased expression of a cloned gene by local mutagenesis of its promoter and ribosome binding site. Nucleic Acids Res (1983) 0.84
Regulated expression of the feline panleukopenia virus P38 promoter on extrachromosomal FPV/EBV chimeric plasmids. J Virol (1989) 0.83
Cloning the complete rIIB gene of bacteriophage T4 and some observations concerning its middle promoters. J Virol (1986) 0.82
Mutational analysis of the Rhizobium meliloti nifA promoter. J Bacteriol (1992) 0.79
A novel prokaryotic promoter identified in the genome of some monopartite begomoviruses. PLoS One (2013) 0.76
Expression of antigenic regions of a trypanosome variable surface glycoprotein in E. coli using Bal-31 nuclease digestion. Nucleic Acids Res (1984) 0.75
Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature (2000) 20.54
GAL4-VP16 is an unusually potent transcriptional activator. Nature (1988) 16.53
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Cell (2001) 15.97
Negative control of p53 by Sir2alpha promotes cell survival under stress. Cell (2001) 15.05
Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans. Nature (2001) 14.44
The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev (1999) 14.43
Deletion analysis of GAL4 defines two transcriptional activating segments. Cell (1987) 14.13
Construction of plasmids carrying the cI gene of bacteriophage lambda. Proc Natl Acad Sci U S A (1976) 13.10
Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast. Methods Enzymol (1983) 12.99
Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science (2000) 12.98
A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site. Proc Natl Acad Sci U S A (1982) 11.42
Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site. Cell (1983) 10.52
Specific DNA binding of GAL4, a positive regulatory protein of yeast. Cell (1985) 10.25
Gene regulation by proteins acting nearby and at a distance. Nature (1986) 9.71
A new class of yeast transcriptional activators. Cell (1987) 9.53
Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1981) 9.45
Five intermediate complexes in transcription initiation by RNA polymerase II. Cell (1989) 9.22
Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene (1983) 8.85
Extrachromosomal rDNA circles--a cause of aging in yeast. Cell (1997) 8.68
A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell (1985) 8.30
A vector for expressing GAL4(1-147) fusions in mammalian cells. Nucleic Acids Res (1989) 8.06
High-efficiency transformation of yeast by electroporation. Methods Enzymol (1991) 7.99
Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG. Mol Cell Biol (1984) 7.83
Negative effect of the transcriptional activator GAL4. Nature (1988) 7.15
Association of phosphatidylinositol kinase activity with polyoma middle-T competent for transformation. Nature (1985) 7.10
Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae. Cell (1984) 6.86
Autoregulation and function of a repressor in bacteriophage lambda. Science (1976) 6.83
Activation of mitogen-activated protein kinase kinase by v-Raf in NIH 3T3 cells and in vitro. Science (1992) 6.80
Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein. Science (1986) 6.79
Structure of the lambda operators. Nature (1973) 6.66
How the lambda repressor and cro work. Cell (1980) 6.65
Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol (1984) 6.23
Sequence of a repressor-binding site in the DNA of bacteriophage lamda. Nature (1974) 6.22
Mechanism of action of the lexA gene product. Proc Natl Acad Sci U S A (1981) 6.13
Polyoma small and middle T antigens and SV40 small t antigen form stable complexes with protein phosphatase 2A. Cell (1990) 6.12
Common elements in growth factor stimulation and oncogenic transformation: 85 kd phosphoprotein and phosphatidylinositol kinase activity. Cell (1987) 5.87
Tyrosine phosphorylation regulates the biochemical and biological properties of pp60c-src. Cell (1987) 5.84
Cooperative binding of lambda repressors to sites separated by integral turns of the DNA helix. Cell (1986) 5.81
ras mediates nerve growth factor receptor modulation of three signal-transducing protein kinases: MAP kinase, Raf-1, and RSK. Cell (1992) 5.77
DNA recognition by GAL4: structure of a protein-DNA complex. Nature (1992) 5.60
Multiple repressor binding at the operators in bacteriophage lambda. Proc Natl Acad Sci U S A (1973) 5.15
Genetic isolation of ADA2: a potential transcriptional adaptor required for function of certain acidic activation domains. Cell (1992) 5.14
Interactions between DNA-bound repressors govern regulation by the lambda phage repressor. Proc Natl Acad Sci U S A (1979) 5.02
Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization. Proc Natl Acad Sci U S A (1984) 4.90
Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA box". Proc Natl Acad Sci U S A (1984) 4.88
Yeast promoters: positive and negative elements. Cell (1984) 4.87
Direct activation of the serine/threonine kinase activity of Raf-1 through tyrosine phosphorylation by the PDGF beta-receptor. Cell (1989) 4.86
Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus. Proc Natl Acad Sci U S A (1976) 4.74
Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family. Cell (1987) 4.70
Each of three "TATA elements" specifies a subset of the transcription initiation sites at the CYC-1 promoter of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1985) 4.69
Recognition of a DNA operator by the repressor of phage 434: a view at high resolution. Science (1988) 4.60
A mechanism for synergistic activation of a mammalian gene by GAL4 derivatives. Nature (1990) 4.57
lambda Repressor and cro--components of an efficient molecular switch. Nature (1981) 4.52
The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80. Cell (1987) 4.49
Sustained dysfunction of antiviral CD8+ T lymphocytes after infection with hepatitis C virus. J Virol (2001) 4.48
Mutants of GAL4 protein altered in an activation function. Cell (1987) 4.44
Identification and characterization of HAP4: a third component of the CCAAT-bound HAP2/HAP3 heteromer. Genes Dev (1989) 4.43
Yeast HAP1 activator binds to two upstream activation sites of different sequence. Cell (1987) 4.37
Mechanism of action of the cro protein of bacteriophage lambda. Proc Natl Acad Sci U S A (1978) 4.34
Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda. Cell (1975) 4.28
Control elements in the DNA of bacteriophage lambda. Cold Spring Harb Symp Quant Biol (1974) 4.23
Specific binding of the lambda phage repressor to lambda DNA. Nature (1967) 4.23
Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A (1999) 4.19
A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene. Nature (1985) 4.08
Presence of an SH2 domain in the actin-binding protein tensin. Science (1991) 4.03
Gene regulation at the right operator (OR) of bacteriophage lambda. II. OR1, OR2, and OR3: their roles in mediating the effects of repressor and cro. J Mol Biol (1980) 4.02
Transcription in yeast activated by a putative amphipathic alpha helix linked to a DNA binding unit. Nature (1988) 3.93
Function of a yeast TATA element-binding protein in a mammalian transcription system. Nature (1988) 3.91
Maximizing gene expression on a plasmid using recombination in vitro. Cell (1978) 3.84
Repressor structure and the mechanism of positive control. Cell (1983) 3.82
GAL4 activates gene expression in mammalian cells. Cell (1988) 3.81
Transformation of chicken cells by the gene encoding the catalytic subunit of PI 3-kinase. Science (1997) 3.73
GAL4 activates transcription in Drosophila. Nature (1988) 3.73
Cooperative DNA binding of the yeast transcriptional activator GAL4. Proc Natl Acad Sci U S A (1988) 3.72
The lambda repressor contains two domains. Proc Natl Acad Sci U S A (1979) 3.68
A general method for maximizing the expression of a cloned gene. Proc Natl Acad Sci U S A (1979) 3.67
GAL4 derivatives function alone and synergistically with mammalian activators in vitro. Cell (1988) 3.67
An amino-terminal fragment of GAL4 binds DNA as a dimer. J Mol Biol (1989) 3.65
Gene regulation at the right operator (OR) bacteriophage lambda. I. OR3 and autogenous negative control by repressor. J Mol Biol (1980) 3.64
MEC1-dependent redistribution of the Sir3 silencing protein from telomeres to DNA double-strand breaks. Cell (1999) 3.62
Functional dissection and sequence of yeast HAP1 activator. Cell (1989) 3.56
Signal transduction from membrane to cytoplasm: growth factors and membrane-bound oncogene products increase Raf-1 phosphorylation and associated protein kinase activity. Proc Natl Acad Sci U S A (1988) 3.52
Accelerated aging and nucleolar fragmentation in yeast sgs1 mutants. Science (1997) 3.48
Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell (1999) 3.46
Yeast HAP1 activator competes with the factor RC2 for binding to the upstream activation site UAS1 of the CYC1 gene. Cell (1987) 3.46
Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell (1997) 3.44
Mutation in the silencing gene SIR4 can delay aging in S. cerevisiae. Cell (1995) 3.37
lac repressor can regulate expression from a hybrid SV40 early promoter containing a lac operator in animal cells. Cell (1987) 3.33
Selective inhibition of activated but not basal transcription by the acidic activation domain of VP16: evidence for transcriptional adaptors. Cell (1990) 3.31
Stringent regulation of stably integrated chloramphenicol acetyl transferase genes by E. coli lac repressor in monkey cells. Cell (1988) 3.29
Generating yeast transcriptional activators containing no yeast protein sequences. Nature (1991) 3.21
Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions. Cell (1989) 3.18
Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences. Proc Natl Acad Sci U S A (1989) 3.18
Oncogenes, growth factors, and signal transduction. N Engl J Med (1989) 3.18
Lambda repressor turns off transcription of its own gene. Proc Natl Acad Sci U S A (1975) 3.12
Molecular biology of aging. Cell (1999) 3.08