Localized biphasic changes in phosphatidylinositol-4,5-bisphosphate at sites of phagocytosis.

PubWeight™: 3.63‹?› | Rank: Top 1%

🔗 View Article (PMC 2150667)

Published in J Cell Biol on December 25, 2000

Authors

R J Botelho1, M Teruel, R Dierckman, R Anderson, A Wells, J D York, T Meyer, S Grinstein

Author Affiliations

1: Cell Biology Program, Hospital for Sick Children, and Department of Biochemistry, University of Toronto, Ontario M5G 1X8, Canada.

Articles citing this

(truncated to the top 100)

TRAM couples endocytosis of Toll-like receptor 4 to the induction of interferon-beta. Nat Immunol (2008) 7.87

Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic. J Cell Biol (2001) 3.70

Shaping cups into phagosomes and macropinosomes. Nat Rev Mol Cell Biol (2008) 3.62

Phosphoinositides: tiny lipids with giant impact on cell regulation. Physiol Rev (2013) 3.21

Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytes. J Cell Biol (2002) 3.16

CD14 controls the LPS-induced endocytosis of Toll-like receptor 4. Cell (2011) 3.00

Electrostatic sequestration of PIP2 on phospholipid membranes by basic/aromatic regions of proteins. Biophys J (2004) 2.66

Cdc42, Rac1, and Rac2 display distinct patterns of activation during phagocytosis. Mol Biol Cell (2004) 2.49

Phosphatidylinositol phosphate kinases put PI4,5P(2) in its place. J Membr Biol (2003) 2.43

Cross-talk unfolded: MARCKS proteins. Biochem J (2002) 2.41

The yeast synaptojanin-like proteins control the cellular distribution of phosphatidylinositol (4,5)-bisphosphate. Mol Biol Cell (2002) 2.39

Inhibition of "self" engulfment through deactivation of myosin-II at the phagocytic synapse between human cells. J Cell Biol (2008) 2.39

Integration of phosphoinositide- and calmodulin-mediated regulation of TRPC6. Mol Cell (2007) 2.33

Restricted accumulation of phosphatidylinositol 3-kinase products in a plasmalemmal subdomain during Fc gamma receptor-mediated phagocytosis. J Cell Biol (2001) 2.28

Subcellular localization of phosphatidylinositol 4,5-bisphosphate using the pleckstrin homology domain of phospholipase C delta1. Biochem J (2002) 2.16

Interdependent assembly of specific regulatory lipids and membrane fusion proteins into the vertex ring domain of docked vacuoles. J Cell Biol (2004) 2.08

A promiscuous lipid-binding protein diversifies the subcellular sites of toll-like receptor signal transduction. Cell (2014) 1.99

The p110δ isoform of the kinase PI(3)K controls the subcellular compartmentalization of TLR4 signaling and protects from endotoxic shock. Nat Immunol (2012) 1.94

Agonist-induced PIP(2) hydrolysis inhibits cortical actin dynamics: regulation at a global but not at a micrometer scale. Mol Biol Cell (2002) 1.93

A polarized Ca2+, diacylglycerol and STIM1 signalling system regulates directed cell migration. Nat Cell Biol (2014) 1.91

Classical transient receptor potential channel 6 (TRPC6) is essential for hypoxic pulmonary vasoconstriction and alveolar gas exchange. Proc Natl Acad Sci U S A (2006) 1.89

ARF6 regulates a plasma membrane pool of phosphatidylinositol(4,5)bisphosphate required for regulated exocytosis. J Cell Biol (2003) 1.82

Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis. J Cell Biol (2005) 1.81

Actin depletion initiates events leading to granule secretion at the immunological synapse. Immunity (2015) 1.80

Myo1c binds tightly and specifically to phosphatidylinositol 4,5-bisphosphate and inositol 1,4,5-trisphosphate. Proc Natl Acad Sci U S A (2006) 1.72

Phosphoinositide binding by the Toll adaptor dMyD88 controls antibacterial responses in Drosophila. Immunity (2012) 1.67

Innate immune pattern recognition: a cell biological perspective. Annu Rev Immunol (2015) 1.66

Presenilin 1 mediates the turnover of telencephalin in hippocampal neurons via an autophagic degradative pathway. J Cell Biol (2004) 1.65

Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis. Blood (2006) 1.52

PI(4,5)P2-dependent microdomain assemblies capture microtubules to promote and control leading edge motility. J Cell Biol (2005) 1.51

The phosphoinositol-3-kinase-protein kinase B/Akt pathway is critical for Pseudomonas aeruginosa strain PAK internalization. Mol Biol Cell (2005) 1.49

Phosphatidylinositol-(4,5)-bisphosphate regulates clathrin-coated pit initiation, stabilization, and size. Mol Biol Cell (2011) 1.41

Contribution of phosphatidylserine to membrane surface charge and protein targeting during phagosome maturation. J Cell Biol (2009) 1.38

Dynamics of cytoskeletal proteins during Fcgamma receptor-mediated phagocytosis in macrophages. Mol Biol Cell (2002) 1.36

Local PIP(2) signals: when, where, and how? Pflugers Arch (2007) 1.35

Biomolecular mimicry in the actin cytoskeleton: mechanisms underlying the cytotoxicity of kabiramide C and related macrolides. Proc Natl Acad Sci U S A (2003) 1.33

Loss of PIP5KIgamma, unlike other PIP5KI isoforms, impairs the integrity of the membrane cytoskeleton in murine megakaryocytes. J Clin Invest (2008) 1.33

Phosphatidylinositol-4,5-bisphosphate-rich plasma membrane patches organize active zones of endocytosis and ruffling in cultured adipocytes. Mol Cell Biol (2004) 1.32

Self inhibition of phagocytosis: the affinity of 'marker of self' CD47 for SIRPalpha dictates potency of inhibition but only at low expression levels. Blood Cells Mol Dis (2010) 1.31

Regulation of endocytic traffic by Rho GTPases. Biochem J (2003) 1.31

Differential association of phosphatidylinositol 3-kinase, SHIP-1, and PTEN with forming phagosomes. Mol Biol Cell (2007) 1.28

Critical role for scaffolding adapter Gab2 in Fc gamma R-mediated phagocytosis. J Cell Biol (2003) 1.28

Expression of phosphatidylinositol (4,5) bisphosphate-specific pleckstrin homology domains alters direction but not the level of axonal transport of mitochondria. Mol Biol Cell (2003) 1.26

A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique. Proc Natl Acad Sci U S A (2009) 1.25

Regulation of presynaptic phosphatidylinositol 4,5-biphosphate by neuronal activity. J Cell Biol (2001) 1.25

Activation of TRPC6 channels is essential for lung ischaemia-reperfusion induced oedema in mice. Nat Commun (2012) 1.25

Essential and unique roles of PIP5K-gamma and -alpha in Fcgamma receptor-mediated phagocytosis. J Cell Biol (2009) 1.23

Massive calcium-activated endocytosis without involvement of classical endocytic proteins. J Gen Physiol (2011) 1.20

Bruton's Tyrosine Kinase (BTK) and Vav1 contribute to Dectin1-dependent phagocytosis of Candida albicans in macrophages. PLoS Pathog (2013) 1.19

Phosphatidylinositol-(4,5)-bisphosphate enables efficient secretion of HIV-1 Tat by infected T-cells. EMBO J (2010) 1.18

Macrophage NADPH oxidase flavocytochrome B localizes to the plasma membrane and Rab11-positive recycling endosomes. J Immunol (2009) 1.17

Mammalian septins are required for phagosome formation. Mol Biol Cell (2008) 1.16

Phosphatidic acid plays a regulatory role in clathrin-mediated endocytosis. Mol Biol Cell (2010) 1.15

Inhibition and redistribution of NHE3, the apical Na+/H+ exchanger, by Clostridium difficile toxin B. J Gen Physiol (2004) 1.15

Regulation of the actin cytoskeleton by phosphatidylinositol 4-phosphate 5 kinases. Pflugers Arch (2007) 1.14

Coronin-1 function is required for phagosome formation. Mol Biol Cell (2005) 1.12

A Cdc42 activation cycle coordinated by PI 3-kinase during Fc receptor-mediated phagocytosis. Mol Biol Cell (2009) 1.11

Pathogen destruction versus intracellular survival: the role of lipids as phagosomal fate determinants. J Clin Invest (2008) 1.10

Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis. Proc Natl Acad Sci U S A (2013) 1.10

Phosphatidylserine dynamics in cellular membranes. Mol Biol Cell (2012) 1.10

Endosomal type Iγ PIP 5-kinase controls EGF receptor lysosomal sorting. Dev Cell (2013) 1.10

Phospholipase D signaling: orchestration by PIP2 and small GTPases. Naunyn Schmiedebergs Arch Pharmacol (2007) 1.09

Lipid binding to the tail domain of vinculin: specificity and the role of the N and C termini. J Biol Chem (2008) 1.09

Evidence for a fence that impedes the diffusion of phosphatidylinositol 4,5-bisphosphate out of the forming phagosomes of macrophages. Mol Biol Cell (2011) 1.09

Molecular modeling of the membrane targeting of phospholipase C pleckstrin homology domains. Protein Sci (2003) 1.08

An electrostatic switch displaces phosphatidylinositol phosphate kinases from the membrane during phagocytosis. J Cell Biol (2009) 1.08

Curvature recognition and force generation in phagocytosis. BMC Biol (2010) 1.08

Arf6 and phosphoinositol-4-phosphate-5-kinase activities permit bypass of the Rac1 requirement for beta1 integrin-mediated bacterial uptake. J Exp Med (2003) 1.08

The receptor tyrosine kinase MerTK activates phospholipase C gamma2 during recognition of apoptotic thymocytes by murine macrophages. J Leukoc Biol (2004) 1.08

Alteration of epithelial structure and function associated with PtdIns(4,5)P2 degradation by a bacterial phosphatase. J Gen Physiol (2007) 1.07

Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets. J Cell Biol (2010) 1.06

Unique targeting of cytosolic phospholipase A2 to plasma membranes mediated by the NADPH oxidase in phagocytes. J Cell Biol (2003) 1.05

Polyphosphoinositides are enriched in plant membrane rafts and form microdomains in the plasma membrane. Plant Physiol (2010) 1.03

Recruitment of OCRL and Inpp5B to phagosomes by Rab5 and APPL1 depletes phosphoinositides and attenuates Akt signaling. Mol Biol Cell (2011) 1.03

SNAP25, but not syntaxin 1A, recycles via an ARF6-regulated pathway in neuroendocrine cells. Mol Biol Cell (2005) 1.03

Coordination of Fc receptor signaling regulates cellular commitment to phagocytosis. Proc Natl Acad Sci U S A (2010) 1.03

Two distinct functions for PI3-kinases in macropinocytosis. J Cell Sci (2013) 1.03

Dynamin regulates focal exocytosis in phagocytosing macrophages. Mol Biol Cell (2003) 1.03

The professional phagocyte Dictyostelium discoideum as a model host for bacterial pathogens. Curr Drug Targets (2011) 1.02

Generation of membrane structures during phagocytosis and chemotaxis of macrophages: role and regulation of the actin cytoskeleton. Immunol Rev (2013) 1.01

The LIM protein Ajuba regulates phosphatidylinositol 4,5-bisphosphate levels in migrating cells through an interaction with and activation of PIPKI alpha. Mol Cell Biol (2005) 1.01

Class I and class III phosphoinositide 3-kinases are required for actin polymerization that propels phagosomes. J Cell Biol (2010) 1.00

Spatial analysis of 3' phosphoinositide signaling in living fibroblasts: I. Uniform stimulation model and bounds on dimensionless groups. Biophys J (2004) 1.00

Annexin A2 regulates phagocytosis of photoreceptor outer segments in the mouse retina. Mol Biol Cell (2009) 0.99

Targeting of protein kinase C-epsilon during Fcgamma receptor-dependent phagocytosis requires the epsilonC1B domain and phospholipase C-gamma1. Mol Biol Cell (2005) 0.99

In situ quantitative imaging of cellular lipids using molecular sensors. Nat Chem (2011) 0.97

SHIP-1 increases early oxidative burst and regulates phagosome maturation in macrophages. J Immunol (2008) 0.97

Phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) specifically induces membrane penetration and deformation by Bin/amphiphysin/Rvs (BAR) domains. J Biol Chem (2012) 0.96

FBP17 Mediates a Common Molecular Step in the Formation of Podosomes and Phagocytic Cups in Macrophages. J Biol Chem (2009) 0.95

Yersinia entry into host cells requires Rab5-dependent dephosphorylation of PI(4,5)P₂ and membrane scission. Cell Host Microbe (2012) 0.95

Phagosome as the organelle linking innate and adaptive immunity. Traffic (2012) 0.92

Heterogeneity in macrophage phagocytosis of Staphylococcus aureus strains: high-throughput scanning cytometry-based analysis. PLoS One (2009) 0.92

Domain formation in phosphatidylinositol monophosphate/phosphatidylcholine mixed vesicles. Biophys J (2004) 0.92

Signaling-dependent immobilization of acylated proteins in the inner monolayer of the plasma membrane. J Cell Biol (2006) 0.92

Photoreceptor phagocytosis is mediated by phosphoinositide signaling. FASEB J (2013) 0.91

Separate functions of gelsolin mediate sequential steps of collagen phagocytosis. Mol Biol Cell (2005) 0.90

CAPS and Munc13 utilize distinct PIP2-linked mechanisms to promote vesicle exocytosis. Mol Biol Cell (2013) 0.89

Filamentous morphology of bacteria delays the timing of phagosome morphogenesis in macrophages. J Cell Biol (2013) 0.89

Unrestricted diffusion of exogenous and endogenous PIP(2 )in baby hamster kidney and Chinese hamster ovary cell plasmalemma. J Membr Biol (2007) 0.89

A growth factor signaling cascade confined to circular ruffles in macrophages. Biol Open (2012) 0.88

Articles cited by this

Visualization of phosphoinositides that bind pleckstrin homology domains: calcium- and agonist-induced dynamic changes and relationship to myo-[3H]inositol-labeled phosphoinositide pools. J Cell Biol (1998) 6.70

Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells. Curr Biol (1998) 5.94

A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages. J Cell Biol (1996) 5.52

Fc receptor biology. Annu Rev Immunol (1997) 5.08

Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation. Cell (1999) 4.98

Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases. Science (1998) 4.92

The small GTP-binding protein Rho regulates a phosphatidylinositol 4-phosphate 5-kinase in mammalian cells. Cell (1994) 4.80

Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion. Cell (2000) 4.45

Specific and high-affinity binding of inositol phosphates to an isolated pleckstrin homology domain. Proc Natl Acad Sci U S A (1995) 4.10

Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals. Cell (1998) 3.90

Rho family GTPases bind to phosphoinositide kinases. J Biol Chem (1995) 3.49

Regulation of phosphoinositide-specific phospholipase C isozymes. J Biol Chem (1997) 3.34

Requirements for both Rac1 and Cdc42 in membrane ruffling and phagocytosis in leukocytes. J Exp Med (1997) 3.32

A requirement for phosphatidylinositol 3-kinase in pseudopod extension. J Biol Chem (1999) 3.08

Specificity and promiscuity in phosphoinositide binding by pleckstrin homology domains. J Biol Chem (1998) 3.07

PIP2 and PIP3: complex roles at the cell surface. Cell (2000) 3.02

Phosphoinositide lipids as signaling molecules: common themes for signal transduction, cytoskeletal regulation, and membrane trafficking. Annu Rev Cell Dev Biol (1998) 3.01

Compartmentalized IgE receptor-mediated signal transduction in living cells. J Cell Biol (1997) 2.91

A critical role for Syk in signal transduction and phagocytosis mediated by Fcgamma receptors on macrophages. J Exp Med (1997) 2.76

Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. J Cell Biol (1998) 2.65

A Rap guanine nucleotide exchange factor enriched highly in the basal ganglia. Proc Natl Acad Sci U S A (1998) 2.47

Phosphatidylinositol 3-kinase-dependent membrane association of the Bruton's tyrosine kinase pleckstrin homology domain visualized in single living cells. J Biol Chem (1999) 2.43

A role for lipid rafts in B cell antigen receptor signaling and antigen targeting. J Exp Med (1999) 2.42

Tyrosine 1101 of Tie2 is the major site of association of p85 and is required for activation of phosphatidylinositol 3-kinase and Akt. Mol Cell Biol (1998) 2.28

Tyrosine phosphorylation of the gamma subunit of Fc gamma receptors, p72syk, and paxillin during Fc receptor-mediated phagocytosis in macrophages. J Biol Chem (1994) 2.17

The Syk protein tyrosine kinase is essential for Fcgamma receptor signaling in macrophages and neutrophils. Mol Cell Biol (1998) 2.10

Ca(2+)-independent F-actin assembly and disassembly during Fc receptor-mediated phagocytosis in mouse macrophages. J Cell Biol (1991) 2.03

Type Ialpha phosphatidylinositol-4-phosphate 5-kinase mediates Rac-dependent actin assembly. Curr Biol (2000) 2.00

A role for MARCKS, the alpha isozyme of protein kinase C and myosin I in zymosan phagocytosis by macrophages. J Exp Med (1995) 1.96

Tyrosine phosphorylation is required for Fc receptor-mediated phagocytosis in mouse macrophages. J Exp Med (1993) 1.91

A requirement for ARF6 in Fcgamma receptor-mediated phagocytosis in macrophages. J Biol Chem (1998) 1.82

A versatile microporation technique for the transfection of cultured CNS neurons. J Neurosci Methods (1999) 1.80

Association of Rap1a and Rap1b proteins with late endocytic/phagocytic compartments and Rap2a with the Golgi complex. J Cell Sci (1994) 1.78

Fc receptor-mediated phagocytosis requires CDC42 and Rac1. EMBO J (1998) 1.78

Ca2+-dependent and Ca2+-independent phagocytosis in human neutrophils. Nature (1985) 1.71

Rho is required for the initiation of calcium signaling and phagocytosis by Fcgamma receptors in macrophages. J Exp Med (1997) 1.71

Involvement of phosphatidylinositol 3-kinase in Fc gamma receptor signaling. J Biol Chem (1994) 1.64

PIPkins1, their substrates and their products: new functions for old enzymes. Biochim Biophys Acta (1998) 1.52

Massive actin polymerization induced by phosphatidylinositol-4-phosphate 5-kinase in vivo. J Biol Chem (1997) 1.51

Membrane trafficking along the phagocytic pathway. Trends Cell Biol (1995) 1.51

A role for gelsolin in actuating epidermal growth factor receptor-mediated cell motility. J Cell Biol (1996) 1.45

Fc-receptor-mediated phagocytosis occurs in macrophages without an increase in average [Ca++]i. J Cell Biol (1986) 1.44

Tyrosine phosphorylation of phospholipase C-gamma 1 induced by cross-linking of the high-affinity or low-affinity Fc receptor for IgG in U937 cells. Proc Natl Acad Sci U S A (1992) 1.42

Stimulation of Fc gamma RIIIA results in phospholipase C-gamma 1 tyrosine phosphorylation and p56lck activation. J Exp Med (1992) 1.41

Signaling pathways in phagocytosis. Bioessays (1999) 1.40

Fc receptor-directed phagocytic stimuli induce transient actin assembly at an early stage of phagocytosis in neutrophil leukocytes. Eur J Cell Biol (1984) 1.35

Mitogenic signaling from the egf receptor is attenuated by a phospholipase C-gamma/protein kinase C feedback mechanism. Mol Biol Cell (1996) 1.35

Immunoglobulin-mediated phagocytosis by human monocytes requires protein kinase C activation. Evidence for protein kinase C translocation to phagosomes. J Biol Chem (1992) 1.34

Phosphatidylinositol-4-phosphate 5-kinase isozymes catalyze the synthesis of 3-phosphate-containing phosphatidylinositol signaling molecules. J Biol Chem (1997) 1.32

Shaping up for shipping out: PLCgamma signaling of morphology changes in EGF-stimulated fibroblast migration. Cell Motil Cytoskeleton (1999) 1.27

The guanine nucleotide exchange factor RasGRP is a high -affinity target for diacylglycerol and phorbol esters. Mol Pharmacol (2000) 1.24

The small Mr Ras-like GTPase Rap1 and the phospholipase C pathway act to regulate phagocytosis in Dictyostelium discoideum. Mol Biol Cell (1999) 1.23

Regulation of inositol lipid kinases by Rho and Rac. Curr Opin Genet Dev (1998) 1.21

beta2-chimaerin is a novel target for diacylglycerol: binding properties and changes in subcellular localization mediated by ligand binding to its C1 domain. Proc Natl Acad Sci U S A (1999) 1.19

Phospholipid-binding protein domains. Biochim Biophys Acta (1998) 1.18

Calcium transients during Fc receptor-mediated and nonspecific phagocytosis by murine peritoneal macrophages. J Cell Biol (1991) 1.09

Expression of the protein kinase C substrate pleckstrin in macrophages: association with phagosomal membranes. J Immunol (1999) 1.07

Fc gamma receptor-mediated activation of phospholipase D regulates macrophage phagocytosis of IgG-opsonized particles. J Immunol (1999) 1.06

Protein kinase C and a calcium-independent phospholipase are required for IgG-mediated phagocytosis by Mono-Mac-6 cells. J Leukoc Biol (1999) 0.91

Novel inositol polyphosphate 5-phosphatase localizes at membrane ruffles. J Biol Chem (1999) 0.91

Articles by these authors

Association of depression and diabetes complications: a meta-analysis. Psychosom Med (2001) 8.30

NHS-wide networking and patient confidentiality. BMJ (1995) 7.38

Range of messenger action of calcium ion and inositol 1,4,5-trisphosphate. Science (1992) 6.72

Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells. Curr Biol (1998) 5.94

Male attractiveness and differential testosterone investment in zebra finch eggs. Science (1999) 5.14

Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction. J Clin Microbiol (1989) 4.46

Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion. Cell (2000) 4.45

A competency model for general practice: implications for selection, training, and development. Br J Gen Pract (2000) 4.25

Concentration of viruses from sewage and excreta on insoluble polyelectrolytes. Appl Microbiol (1969) 4.17

Cell adhesion and motility depend on nanoscale RGD clustering. J Cell Sci (2000) 4.13

Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals. Cell (1998) 3.90

Worldwide variations in the prevalence of symptoms of atopic eczema in the International Study of Asthma and Allergies in Childhood. J Allergy Clin Immunol (1999) 3.87

Inhibition of the Abl protein-tyrosine kinase in vitro and in vivo by a 2-phenylaminopyrimidine derivative. Cancer Res (1996) 3.77

Physical activity in patients with COPD. Eur Respir J (2008) 3.75

Distinct roles of class I and class III phosphatidylinositol 3-kinases in phagosome formation and maturation. J Cell Biol (2001) 3.63

A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control. Science (2000) 3.63

Pneumococcal vaccine campaign based in general practice. BMJ (1997) 3.50

Frequent loss of Shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation. Infect Immun (1992) 3.47

A reproducible microanalytical method for the detection of specific RNA sequences by dot-blot hybridization. Anal Biochem (1984) 3.47

Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation. Science (1999) 3.42

The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange. Nature (1998) 3.41

Why do symptomatic patients delay obtaining care for tuberculosis? Am J Respir Crit Care Med (1998) 3.30

Virus isolations from sewage and from a stream receiving effluents of sewage treatment plants. Bull World Health Organ (1970) 3.27

Spatial sensing in fibroblasts mediated by 3' phosphoinositides. J Cell Biol (2000) 3.21

Chaos, persistence, and evolution of strain structure in antigenically diverse infectious agents. Science (1998) 3.19

Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nat Biotechnol (1996) 3.19

Prospective randomized controlled trial of extended-release oxybutynin chloride and tolterodine tartrate in the treatment of overactive bladder: results of the OBJECT Study. Mayo Clin Proc (2001) 3.17

Black-white mortality differences by family income. Lancet (1992) 3.15

Efficient chromosome capture requires a bias in the 'search-and-capture' process during mitotic-spindle assembly. Curr Biol (2005) 3.08

The effect of antibody-dependent enhancement on the transmission dynamics and persistence of multiple-strain pathogens. Proc Natl Acad Sci U S A (1999) 3.06

Dual role of calmodulin in autophosphorylation of multifunctional CaM kinase may underlie decoding of calcium signals. Neuron (1994) 3.00

Conformational maturation of CFTR but not its mutant counterpart (delta F508) occurs in the endoplasmic reticulum and requires ATP. EMBO J (1994) 2.93

Compartmentalized IgE receptor-mediated signal transduction in living cells. J Cell Biol (1997) 2.91

Antigen receptor-induced activation and cytoskeletal rearrangement are impaired in Wiskott-Aldrich syndrome protein-deficient lymphocytes. J Exp Med (1999) 2.81

SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases. J Biol Chem (1999) 2.75

Mechanisms of regulation of the Na+/H+ exchanger. J Membr Biol (1986) 2.72

Disruption of KCC2 reveals an essential role of K-Cl cotransport already in early synaptic inhibition. Neuron (2001) 2.71

The delta F508 mutation decreases the stability of cystic fibrosis transmembrane conductance regulator in the plasma membrane. Determination of functional half-lives on transfected cells. J Biol Chem (1993) 2.65

Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. J Cell Biol (1998) 2.65

Evaluation of oligonucleotide probes for identification of shiga-like-toxin-producing Escherichia coli. J Clin Microbiol (1989) 2.62

Coupling between intracellular Ca2+ stores and the Ca2+ permeability of the plasma membrane. Comparison of the effects of thapsigargin, 2,5-di-(tert-butyl)-1,4-hydroquinone, and cyclopiazonic acid in rat thymic lymphocytes. J Biol Chem (1991) 2.54

Community acquired pneumonia: aetiology and usefulness of severity criteria on admission. Thorax (1996) 2.46

Randomized, prospective, placebo-controlled trial of bosentan in interstitial lung disease secondary to systemic sclerosis. Arthritis Rheum (2010) 2.37

Comparison of custom and prefabricated orthoses in the initial treatment of proximal plantar fasciitis. Foot Ankle Int (1999) 2.37

Epidermal growth factor receptor activation of calpain is required for fibroblast motility and occurs via an ERK/MAP kinase signaling pathway. J Biol Chem (2000) 2.37

Prevalence and diagnosis of chronic respiratory symptoms in adults. BMJ (1989) 2.28

Natural resistance to intracellular infections: natural resistance-associated macrophage protein 1 (Nramp1) functions as a pH-dependent manganese transporter at the phagosomal membrane. J Exp Med (2000) 2.28

Tyrosine 1101 of Tie2 is the major site of association of p85 and is required for activation of phosphatidylinositol 3-kinase and Akt. Mol Cell Biol (1998) 2.28

Restricted accumulation of phosphatidylinositol 3-kinase products in a plasmalemmal subdomain during Fc gamma receptor-mediated phagocytosis. J Cell Biol (2001) 2.28

Translocation and reversible localization of signaling proteins: a dynamic future for signal transduction. Cell (2000) 2.27

Cytoplasmic pH regulation in thymic lymphocytes by an amiloride-sensitive Na+/H+ antiport. J Gen Physiol (1984) 2.23

Calreticulin is essential for integrin-mediated calcium signalling and cell adhesion. Nature (1997) 2.21

Nucleocytoplasmic translocation of Stat1 is regulated by a leucine-rich export signal in the coiled-coil domain. Proc Natl Acad Sci U S A (2000) 2.20

Crystal structure of the mitotic spindle kinesin Eg5 reveals a novel conformation of the neck-linker. J Biol Chem (2001) 2.18

Subcellular targeting by membrane lipids. Curr Opin Cell Biol (2001) 2.18

Membrane proximal ERK signaling is required for M-calpain activation downstream of epidermal growth factor receptor signaling. J Biol Chem (2001) 2.16

Guidelines for the management of gastroenteropancreatic neuroendocrine (including carcinoid) tumours (NETs). Gut (2011) 2.14

Venous thromboembolic complications (VTE) in children: first analyses of the Canadian Registry of VTE. Blood (1994) 2.14

Point mutations of the p150 subunit of dynactin (DCTN1) gene in ALS. Neurology (2004) 2.14

CaMKIIbeta functions as an F-actin targeting module that localizes CaMKIIalpha/beta heterooligomers to dendritic spines. Neuron (1998) 2.13

Gemcitabine alone or in combination with cisplatin in patients with advanced or metastatic cholangiocarcinomas or other biliary tract tumours: a multicentre randomised phase II study - The UK ABC-01 Study. Br J Cancer (2009) 2.13

Na+/H+ exchangers of mammalian cells. J Biol Chem (1997) 2.12

Epidermal growth factor receptor-mediated cell motility: phospholipase C activity is required, but mitogen-activated protein kinase activity is not sufficient for induced cell movement. J Cell Biol (1994) 2.12

Regulation of proliferation-survival decisions during tumor cell hypoxia. Mol Cell Biol (1998) 2.11

Focal exocytosis of VAMP3-containing vesicles at sites of phagosome formation. J Cell Biol (2000) 2.10

The Syk protein tyrosine kinase is essential for Fcgamma receptor signaling in macrophages and neutrophils. Mol Cell Biol (1998) 2.10

Epidermal growth factor and membrane trafficking. EGF receptor activation of endocytosis requires Rab5a. J Cell Biol (2000) 2.09

Phosphatidylinositol polyphosphate binding to the mammalian septin H5 is modulated by GTP. Curr Biol (2000) 2.09

Epidermal growth factor activates m-calpain (calpain II), at least in part, by extracellular signal-regulated kinase-mediated phosphorylation. Mol Cell Biol (2004) 2.08

First trimester biochemical screening for trisomy 21: the role of free beta hCG, alpha fetoprotein and pregnancy associated plasma protein A. Ann Clin Biochem (1994) 2.06

Age-related changes underlie switch in netrin-1 responsiveness as growth cones advance along visual pathway. Nat Neurosci (2002) 2.06

Ty1-copia group retrotransposons are ubiquitous and heterogeneous in higher plants. Nucleic Acids Res (1992) 2.05

Chlamydia trachomatis, infertility, and population growth in sub-Saharan Africa. Sex Transm Dis (1993) 2.05

Identification, cloning, and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to cytoskeletal protein 4.1. Proc Natl Acad Sci U S A (1991) 2.04

Evaluation of the Abbott LCx ligase chain reaction assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine and genital swab specimens from a sexually transmitted disease clinic population. J Clin Microbiol (1998) 2.04

AIDS, HIV and the Sunday Times. Nature (1994) 2.02

A placebo-controlled trial of ketotifen (HC 20-511, Sandoz) in allergen induced asthma and comparison with disodium cromoglycate. Clin Allergy (1979) 1.98

Calcium-induced restructuring of nuclear envelope and endoplasmic reticulum calcium stores. Cell (1997) 1.96

Internal trafficking and surface mobility of a functionally intact beta2-adrenergic receptor-green fluorescent protein conjugate. Mol Pharmacol (1997) 1.96

Psychometric considerations in evaluating health-related quality of life measures. Qual Life Res (1993) 1.95

An outbreak of nosocomial infection due to multiply resistant Serratia marcescens: evidence of interhospital spread. J Infect Dis (1976) 1.95

Two psychological treatments for hypochondriasis. A randomised controlled trial. Br J Psychiatry (1998) 1.95

A survey of the demographics of blood use. Transfus Med (2007) 1.95

Phagosomal acidification is mediated by a vacuolar-type H(+)-ATPase in murine macrophages. J Biol Chem (1990) 1.94

Retracted Selective inhibition of the platelet-derived growth factor signal transduction pathway by a protein-tyrosine kinase inhibitor of the 2-phenylaminopyrimidine class. Proc Natl Acad Sci U S A (1995) 1.93

Cancer screening guidelines. Am Fam Physician (2001) 1.89

Methamphetamine use and sexual and injection risk behaviors among out-of-treatment injection drug users. Am J Drug Alcohol Abuse (1999) 1.88

A precision measurement of the mass of the top quark. Nature (2004) 1.87

Outcome of pediatric thromboembolic disease: a report from the Canadian Childhood Thrombophilia Registry. Pediatr Res (2000) 1.86

Comparison of aerobic exercise, clomipramine, and placebo in the treatment of panic disorder. Am J Psychiatry (1998) 1.86

Ethnic differences in stroke mortality between non-Hispanic whites, Hispanic whites, and blacks. The National Longitudinal Mortality Study. Stroke (1994) 1.85

Hemolytic uremic syndrome and diarrhea in Argentine children: the role of Shiga-like toxins. J Infect Dis (1989) 1.82

Evaluation of social status as a contributing factor to the stroke belt region of the United States. Stroke (1997) 1.81

A versatile microporation technique for the transfection of cultured CNS neurons. J Neurosci Methods (1999) 1.80

Mechanism of acidification of the trans-Golgi network (TGN). In situ measurements of pH using retrieval of TGN38 and furin from the cell surface. J Biol Chem (1998) 1.78

Developing and evaluating cross-cultural instruments from minimum requirements to optimal models. Qual Life Res (1993) 1.78