Published in Proc Natl Acad Sci U S A on February 03, 2005
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature (2010) 9.95
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals. Nat Methods (2008) 6.51
Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (2010) 4.69
Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application. Nat Rev Drug Discov (2010) 4.25
Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions. J Cell Biol (2010) 3.61
Molecular biology. Use and abuse of RNAi to study mammalian gene function. Science (2012) 2.33
Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material. Nat Cell Biol (2012) 2.19
Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A. J Cell Biol (2008) 1.92
Vigilance and validation: Keys to success in RNAi screening. ACS Chem Biol (2010) 1.85
A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. PLoS Biol (2010) 1.77
A novel role of myosin VI in human prostate cancer. Am J Pathol (2006) 1.59
RNAi screening comes of age: improved techniques and complementary approaches. Nat Rev Mol Cell Biol (2014) 1.54
Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division. EMBO J (2009) 1.51
RNAi screening: new approaches, understandings, and organisms. Wiley Interdiscip Rev RNA (2011) 1.48
Small-molecule and mutational analysis of allosteric Eg5 inhibition by monastrol. BMC Chem Biol (2006) 1.19
TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle. J Cell Biol (2011) 1.17
Tandem affinity purification of functional TAP-tagged proteins from human cells. Nat Protoc (2007) 1.15
Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels. Biotechniques (2012) 1.15
A domesticated transposon mediates the effects of a single-nucleotide polymorphism responsible for enhanced muscle growth. EMBO Rep (2010) 1.14
A genomic toolkit to investigate kinesin and myosin motor function in cells. Nat Cell Biol (2013) 1.11
Cattle mammary bioreactor generated by a novel procedure of transgenic cloning for large-scale production of functional human lactoferrin. PLoS One (2008) 1.11
SKIP counteracts p53-mediated apoptosis via selective regulation of p21Cip1 mRNA splicing. Genes Dev (2011) 1.10
RAS Synthetic Lethal Screens Revisited: Still Seeking the Elusive Prize? Clin Cancer Res (2015) 1.08
Cross-species RNAi rescue platform in Drosophila melanogaster. Genetics (2009) 1.06
Dynamic reorganization of Eg5 in the mammalian spindle throughout mitosis requires dynein and TPX2. Mol Biol Cell (2012) 1.05
In vivo RNAi rescue in Drosophila melanogaster with genomic transgenes from Drosophila pseudoobscura. PLoS One (2010) 1.04
Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives. Biotechniques (2015) 1.00
High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes. Nat Methods (2011) 0.98
The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. J Mol Biol (2007) 0.97
Triple SILAC to determine stimulus specific interactions in the Wnt pathway. J Proteome Res (2011) 0.91
SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs. EMBO J (2014) 0.91
Stable silencing of SNAP-25 in PC12 cells by RNA interference. BMC Neurosci (2006) 0.88
CEP192 interacts physically and functionally with the K63-deubiquitinase CYLD to promote mitotic spindle assembly. Cell Cycle (2012) 0.87
From RNAi screens to molecular function in embryonic stem cells. Stem Cell Rev (2012) 0.85
Short-hairpin RNA-mediated stable silencing of Grb2 impairs cell growth and DNA synthesis. Biochem Biophys Res Commun (2007) 0.84
Dual expression lentiviral vectors for concurrent RNA interference and rescue. J Surg Res (2009) 0.80
Construction and application of an inducible system for homogenous expression levels in bulk cell lines. PLoS One (2009) 0.79
MISSION esiRNA for RNAi screening in mammalian cells. J Vis Exp (2010) 0.78
Common pitfalls in preclinical cancer target validation. Nat Rev Cancer (2017) 0.77
HP1BP3, a Chromatin Retention Factor for Co-transcriptional MicroRNA Processing. Mol Cell (2016) 0.77
Response to Shuda et al. J Invest Dermatol (2013) 0.75
Type I γ Phosphatidylinositol Phosphate 5-Kinase i5 Controls the Ubiquitination and Degradation of the Tumor Suppressor Mitogen-inducible Gene 6. J Biol Chem (2016) 0.75
Multimerization properties of PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements. Nucleic Acids Res (2017) 0.75
Initial sequencing and comparative analysis of the mouse genome. Nature (2002) 96.15
Green fluorescent protein as a marker for gene expression. Science (1994) 34.55
Global analysis of protein localization in budding yeast. Nature (2003) 33.22
Expression profiling reveals off-target gene regulation by RNAi. Nat Biotechnol (2003) 16.63
siRNAs can function as miRNAs. Genes Dev (2003) 16.08
A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet (1998) 15.27
Evolutionary parameters of the transcribed mammalian genome: an analysis of 2,820 orthologous rodent and human sequences. Proc Natl Acad Sci U S A (1998) 11.90
Proteomic characterization of the human centrosome by protein correlation profiling. Nature (2003) 8.74
Activation of the interferon system by short-interfering RNAs. Nat Cell Biol (2003) 8.63
Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet (2001) 8.35
Killing the messenger: short RNAs that silence gene expression. Nat Rev Mol Cell Biol (2003) 7.38
Unlocking the potential of the human genome with RNA interference. Nature (2004) 6.70
Induction of an interferon response by RNAi vectors in mammalian cells. Nat Genet (2003) 6.49
Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells. Proc Natl Acad Sci U S A (2004) 4.85
DNA cloning by homologous recombination in Escherichia coli. Nat Biotechnol (2000) 4.75
Interferon induction by siRNAs and ssRNAs synthesized by phage polymerase. Nat Biotechnol (2004) 3.85
An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature (2004) 3.69
Humanized xenobiotic response in mice expressing nuclear receptor SXR. Nature (2000) 3.41
Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs). RNA (2004) 3.16
A targeting motif involved in sodium channel clustering at the axonal initial segment. Science (2003) 3.02
Systematic genome-wide screens of gene function. Nat Rev Genet (2004) 2.92
Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proc Natl Acad Sci U S A (2002) 2.85
Techniques: Recombinogenic engineering--new options for cloning and manipulating DNA. Trends Biochem Sci (2001) 2.79
Size matters: use of YACs, BACs and PACs in transgenic animals. Transgenic Res (2001) 2.62
Centrosome maturation and duplication in C. elegans require the coiled-coil protein SPD-2. Dev Cell (2004) 2.60
The Caenorhabditis elegans centrosomal protein SPD-2 is required for both pericentriolar material recruitment and centriole duplication. Curr Biol (2004) 2.44
Noise amidst the silence: off-target effects of siRNAs? Trends Genet (2004) 2.38
Conservation of human alternative splice events in mouse. Nucleic Acids Res (2003) 2.35
Whither RNAi? Nat Cell Biol (2003) 2.19
Nuclear coactivator-62 kDa/Ski-interacting protein is a nuclear matrix-associated coactivator that may couple vitamin D receptor-mediated transcription and RNA splicing. J Biol Chem (2003) 1.80
Of mice and men: species variations of Toll-like receptor expression. Trends Immunol (2002) 1.69
Alternative approaches for efficient inhibition of hepatitis C virus RNA replication by small interfering RNAs. J Virol (2004) 1.61
Overexpression and mislocalization of a tail-anchored GFP redefines the identity of peroxisomal ER. Traffic (2003) 1.53
Cloning of murine RNA polymerase I-specific TAF factors: conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1. Proc Natl Acad Sci U S A (1997) 1.48
Determinants of interferon-stimulated gene induction by RNAi vectors. Differentiation (2004) 1.45
Localizing the proteome. Genome Biol (2003) 1.31
Species-specific organization of CpG island promoters at mammalian homologous genes. EMBO Rep (2001) 1.17
Confirming specificity of RNAi in mammalian cells. Sci STKE (2002) 1.07
Gene dosage in mice--BAC to the future. Nat Genet (1999) 0.97
Genomic organization and expression of the human tumorous imaginal disc (TID1) gene. Gene (2001) 0.92
Overexpression of neurofilament H disrupts normal cell structure and function. J Neurosci Res (2002) 0.89
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature (2010) 9.95
Orchestration of the DNA-damage response by the RNF8 ubiquitin ligase. Science (2007) 7.61
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals. Nat Methods (2008) 6.51
The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage. Cell (2009) 5.96
Minimizing the risk of reporting false positives in large-scale RNAi screens. Nat Methods (2006) 5.96
Germline P granules are liquid droplets that localize by controlled dissolution/condensation. Science (2009) 5.55
Systems survey of endocytosis by multiparametric image analysis. Nature (2010) 5.03
Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (2010) 4.69
Dynamics and mechanics of the microtubule plus end. Nature (2003) 4.68
Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers. Cell (2010) 4.31
Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments. Science (2011) 4.26
Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat Cell Biol (2006) 4.13
Genome-scale RNAi profiling of cell division in human tissue culture cells. Nat Cell Biol (2007) 3.91
Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I. Nature (2006) 3.81
An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature (2004) 3.69
Predictive models of molecular machines involved in Caenorhabditis elegans early embryogenesis. Nature (2005) 3.62
Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions. J Cell Biol (2010) 3.61
Centriole assembly in Caenorhabditis elegans. Nature (2006) 3.58
A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity. Cell Stem Cell (2009) 3.55
A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility. Cell (2013) 3.53
XMAP215 is a processive microtubule polymerase. Cell (2008) 3.33
Centriole assembly requires both centriolar and pericentriolar material proteins. Dev Cell (2004) 3.23
SAS-4 is a C. elegans centriolar protein that controls centrosome size. Cell (2003) 3.19
HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity. Curr Biol (2009) 2.98
The distribution of active force generators controls mitotic spindle position. Science (2003) 2.97
Live-cell imaging RNAi screen identifies PP2A-B55alpha and importin-beta1 as key mitotic exit regulators in human cells. Nat Cell Biol (2010) 2.91
Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proc Natl Acad Sci U S A (2002) 2.85
Genome-wide resources of endoribonuclease-prepared short interfering RNAs for specific loss-of-function studies. Nat Methods (2007) 2.83
HIV-1 proviral DNA excision using an evolved recombinase. Science (2007) 2.81
The AP-1A and AP-1B clathrin adaptor complexes define biochemically and functionally distinct membrane domains. J Cell Biol (2003) 2.78
Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding. Nature (2011) 2.68
Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Curr Biol (2006) 2.67
A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue. Cell (2011) 2.64
Microtubule polymerases and depolymerases. Curr Opin Cell Biol (2006) 2.64
Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis. J Cell Biol (2005) 2.57
Genome-wide identification of binding sites defines distinct functions for Caenorhabditis elegans PHA-4/FOXA in development and environmental response. PLoS Genet (2010) 2.46
Katanin disrupts the microtubule lattice and increases polymer number in C. elegans meiosis. Curr Biol (2006) 2.45
The Caenorhabditis elegans centrosomal protein SPD-2 is required for both pericentriolar material recruitment and centriole duplication. Curr Biol (2004) 2.44
Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes. Proc Natl Acad Sci U S A (2011) 2.44
Diverse transcription factor binding features revealed by genome-wide ChIP-seq in C. elegans. Genome Res (2010) 2.30
Limiting amounts of centrosome material set centrosome size in C. elegans embryos. Curr Biol (2011) 2.25
A protein domain-based interactome network for C. elegans early embryogenesis. Cell (2008) 2.23
Centrosome size sets mitotic spindle length in Caenorhabditis elegans embryos. Curr Biol (2010) 2.23
Asymmetric cell division in C. elegans: cortical polarity and spindle positioning. Annu Rev Cell Dev Biol (2004) 2.22
A cytokinesis furrow is positioned by two consecutive signals. Nature (2005) 2.21
Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material. Nat Cell Biol (2012) 2.19
GTP binding induces filament assembly of a recombinant septin. Curr Biol (2002) 2.16
Spindle positioning by cortical pulling forces. Dev Cell (2005) 2.14
Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods. EMBO J (2011) 2.14
The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependent. J Cell Biol (2002) 2.13
Sororin mediates sister chromatid cohesion by antagonizing Wapl. Cell (2010) 2.12
A genome-scale resource for in vivo tag-based protein function exploration in C. elegans. Cell (2012) 2.07
Identification and characterization of factors required for microtubule growth and nucleation in the early C. elegans embryo. Dev Cell (2005) 2.02
The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C. elegans and S. cerevisiae. Nature (2005) 2.01
DEQOR: a web-based tool for the design and quality control of siRNAs. Nucleic Acids Res (2004) 1.97
Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Dis Model Mech (2009) 1.97
Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A. J Cell Biol (2008) 1.92
LET-99, GOA-1/GPA-16, and GPR-1/2 are required for aster-positioned cytokinesis. Curr Biol (2006) 1.91
The mammalian SPD-2 ortholog Cep192 regulates centrosome biogenesis. Curr Biol (2008) 1.88
Polarization of PAR proteins by advective triggering of a pattern-forming system. Science (2011) 1.88
XMAP215: a key component of the dynamic microtubule cytoskeleton. Trends Cell Biol (2002) 1.87
Centrosomes direct cell polarity independently of microtubule assembly in C. elegans embryos. Nature (2004) 1.85
Aurora B controls the association of condensin I but not condensin II with mitotic chromosomes. J Cell Sci (2007) 1.78
A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. PLoS Biol (2010) 1.77
CDC-42 and RHO-1 coordinate acto-myosin contractility and PAR protein localization during polarity establishment in C. elegans embryos. Development (2006) 1.74
Organelle growth control through limiting pools of cytoplasmic components. Curr Biol (2012) 1.71
Aurora A activates D-TACC-Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules. J Cell Biol (2005) 1.70
Human regulatory T cells induce T-lymphocyte senescence. Blood (2012) 1.70
Selective expression of the Cre recombinase in late-stage thymocytes using the distal promoter of the Lck gene. J Immunol (2005) 1.68
Caenorhabditis elegans TAC-1 and ZYG-9 form a complex that is essential for long astral and spindle microtubules. Curr Biol (2003) 1.67
The human cap-binding complex is functionally connected to the nuclear RNA exosome. Nat Struct Mol Biol (2013) 1.67
Cell division. WormBook (2006) 1.63
Transferrin receptor recycling in the absence of perinuclear recycling endosomes. J Cell Biol (2002) 1.62
Bis{tris-[3-(2-pyrid-yl)-1H-pyrazole]manganese(II)} dodeca-molybdo(V,VI)phosphate hexa-hydrate. Acta Crystallogr Sect E Struct Rep Online (2010) 1.62
Production of endoribonuclease-prepared short interfering RNAs for gene silencing in mammalian cells. Nat Methods (2005) 1.62
Growth, fluctuation and switching at microtubule plus ends. Nat Rev Mol Cell Biol (2009) 1.62
Alternative approaches for efficient inhibition of hepatitis C virus RNA replication by small interfering RNAs. J Virol (2004) 1.61