Four distinct structural domains in Clostridium difficile toxin B visualized using SAXS.

PubWeight™: 1.15‹?› | Rank: Top 10%

🔗 View Article (PMID 20070948)

Published in J Mol Biol on January 11, 2010

Authors

David Albesa-Jové1, Thomas Bertrand, Elisabeth P Carpenter, Gemma V Swain, Jenson Lim, Jiancheng Zhang, Lesley F Haire, Nishi Vasisht, Veit Braun, Anton Lange, Christoph von Eichel-Streiber, Dmitri I Svergun, Neil F Fairweather, Katherine A Brown

Author Affiliations

1: Department of Life Sciences, Division of Cell and Molecular Biology, Centre for Molecular Microbiology and Infection, Flowers Building, Exhibition Road, Imperial College London, London SW7 2AZ, UK.

Articles citing this

Clinical Clostridium difficile: clonality and pathogenicity locus diversity. PLoS One (2011) 2.04

Diagnosis of Clostridium difficile infection: an ongoing conundrum for clinicians and for clinical laboratories. Clin Microbiol Rev (2013) 1.85

Structural organization of the functional domains of Clostridium difficile toxins A and B. Proc Natl Acad Sci U S A (2010) 1.44

Variations in TcdB activity and the hypervirulence of emerging strains of Clostridium difficile. PLoS Pathog (2010) 1.26

Toward a structural understanding of Clostridium difficile toxins A and B. Front Cell Infect Microbiol (2012) 1.22

The enterotoxicity of Clostridium difficile toxins. Toxins (Basel) (2010) 1.07

Mechanism of action and epitopes of Clostridium difficile toxin B-neutralizing antibody bezlotoxumab revealed by X-ray crystallography. J Biol Chem (2014) 1.04

Structural basis for antibody recognition in the receptor-binding domains of toxins A and B from Clostridium difficile. J Biol Chem (2013) 0.90

Cytotoxicity of Clostridium difficile toxin B does not require cysteine protease-mediated autocleavage and release of the glucosyltransferase domain into the host cell cytosol. Pathog Dis (2013) 0.90

Toxin-specific antibodies for the treatment of Clostridium difficile: current status and future perspectives. Toxins (Basel) (2010) 0.88

Crystal structure of Clostridium difficile toxin A. Nat Microbiol (2016) 0.87

Critical roles of Clostridium difficile toxin B enzymatic activities in pathogenesis. Infect Immun (2014) 0.86

The combined repetitive oligopeptides of clostridium difficile toxin A counteract premature cleavage of the glucosyl-transferase domain by stabilizing protein conformation. Toxins (Basel) (2014) 0.85

Protective antibody responses against Clostridium difficile elicited by a DNA vaccine expressing the enzymatic domain of toxin B. Hum Vaccin Immunother (2012) 0.84

An update on antibody-based immunotherapies for Clostridium difficile infection. Clin Exp Gastroenterol (2016) 0.80

Autoproteolytic activation of bacterial toxins. Toxins (Basel) (2010) 0.79

Masking autoprocessing of Clostridium difficile toxin A by the C-terminus combined repetitive oligo peptides. Biochem Biophys Res Commun (2015) 0.79

Clostridium difficile Toxins A and B: Insights into Pathogenic Properties and Extraintestinal Effects. Toxins (Basel) (2016) 0.77

Biochemical and Immunological Characterization of Truncated Fragments of the Receptor-Binding Domains of C. difficile Toxin A. PLoS One (2015) 0.77

Utility of Clostridium difficile toxin B for inducing anti-tumor immunity. PLoS One (2014) 0.76

Intrinsic Toxin-Derived Peptides Destabilize and Inactivate Clostridium difficile TcdB. MBio (2017) 0.75

Novel Clostridium difficile Anti-Toxin (TcdA and TcdB) Humanized Monoclonal Antibodies Demonstrate In Vitro Neutralization across a Broad Spectrum of Clinical Strains and In Vivo Potency in a Hamster Spore Challenge Model. PLoS One (2016) 0.75

Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19. Acta Crystallogr Sect F Struct Biol Cryst Commun (2011) 0.75

Articles by these authors

The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat Genet (2006) 8.02

A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins. Science (2011) 7.86

Haemagglutinin mutations responsible for the binding of H5N1 influenza A viruses to human-type receptors. Nature (2006) 7.56

Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc Natl Acad Sci U S A (2004) 7.30

Global rigid body modeling of macromolecular complexes against small-angle scattering data. Biophys J (2005) 7.10

The molecular basis for phosphodependent substrate targeting and regulation of Plks by the Polo-box domain. Cell (2003) 7.04

Structural characterization of flexible proteins using small-angle X-ray scattering. J Am Chem Soc (2007) 6.53

The structure of H5N1 avian influenza neuraminidase suggests new opportunities for drug design. Nature (2006) 6.00

HIV-1 restriction factor SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase. Nature (2011) 5.28

Small-angle scattering: a view on the properties, structures and structural changes of biological macromolecules in solution. Q Rev Biophys (2003) 4.54

Structure of mammalian AMPK and its regulation by ADP. Nature (2011) 4.27

Structure and catalytic mechanism of the human histone methyltransferase SET7/9. Nature (2003) 4.00

Crystal structures of oseltamivir-resistant influenza virus neuraminidase mutants. Nature (2008) 3.79

Structural characterization of proteins and complexes using small-angle X-ray solution scattering. J Struct Biol (2010) 3.44

Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter. Science (2008) 3.39

Antibiotic treatment of clostridium difficile carrier mice triggers a supershedder state, spore-mediated transmission, and severe disease in immunocompromised hosts. Infect Immun (2009) 3.36

Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer. Nat Struct Mol Biol (2004) 3.26

High-resolution structure of a retroviral capsid hexameric amino-terminal domain. Nature (2004) 2.84

Activities of NXL104 combinations with ceftazidime and aztreonam against carbapenemase-Producing Enterobacteriaceae. Antimicrob Agents Chemother (2010) 2.54

Analysis of X-ray and neutron scattering from biomacromolecular solutions. Curr Opin Struct Biol (2007) 2.50

Arrival of Klebsiella pneumoniae producing KPC carbapenemase in the United Kingdom. J Antimicrob Chemother (2008) 2.35

Receptor binding by an H7N9 influenza virus from humans. Nature (2013) 2.33

Expression and prognostic relevance of annexin A3 in prostate cancer. Eur Urol (2008) 2.32

Structural basis for engagement by complement factor H of C3b on a self surface. Nat Struct Mol Biol (2011) 2.32

Comparison of BD Phoenix, Vitek 2, and MicroScan automated systems for detection and inference of mechanisms responsible for carbapenem resistance in Enterobacteriaceae. J Clin Microbiol (2010) 2.30

HIV and STD status among MSM and attitudes about Internet partner notification for STD exposure. Sex Transm Dis (2008) 2.18

Structure of tumor suppressor p53 and its intrinsically disordered N-terminal transactivation domain. Proc Natl Acad Sci U S A (2008) 2.14

Autocatalytic cleavage of Clostridium difficile toxin B. Nature (2007) 2.11

Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex. Proc Natl Acad Sci U S A (2007) 2.09

A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damage. Cell (2009) 2.07

Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2. EMBO J (2010) 2.05

Publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution. Acta Crystallogr D Biol Crystallogr (2012) 2.04

The Clostridium difficile spo0A gene is a persistence and transmission factor. Infect Immun (2012) 2.02

Structural and functional versatility of the FHA domain in DNA-damage signaling by the tumor suppressor kinase Chk2. Mol Cell (2002) 1.99

Structures of receptor complexes formed by hemagglutinins from the Asian Influenza pandemic of 1957. Proc Natl Acad Sci U S A (2009) 1.98

Impact and progress in small and wide angle X-ray scattering (SAXS and WAXS). Curr Opin Struct Biol (2013) 1.90

What remains against carbapenem-resistant Enterobacteriaceae? Evaluation of chloramphenicol, ciprofloxacin, colistin, fosfomycin, minocycline, nitrofurantoin, temocillin and tigecycline. Int J Antimicrob Agents (2011) 1.89

Structural basis of hepatocyte growth factor/scatter factor and MET signalling. Proc Natl Acad Sci U S A (2006) 1.85

Prevalence of faecal carriage of Enterobacteriaceae with NDM-1 carbapenemase at military hospitals in Pakistan, and evaluation of two chromogenic media. J Antimicrob Chemother (2011) 1.82

Structural basis for autoinhibition of ESCRT-III CHMP3. J Mol Biol (2008) 1.78

Molecules incorporating a benzothiazole core scaffold inhibit the N-myristoyltransferase of Plasmodium falciparum. Biochem J (2007) 1.75

X-ray scattering study of activated Arp2/3 complex with bound actin-WCA. Structure (2008) 1.75

The structure and binding behavior of the bacterial cell surface layer protein SbsC. Structure (2008) 1.72

Structural insights into the molecular organization of the S-layer from Clostridium difficile. Mol Microbiol (2009) 1.71

The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol (2012) 1.69

Structural basis of dcp2 recognition and activation by dcp1. Mol Cell (2008) 1.65

Protein farnesyl and N-myristoyl transferases: piggy-back medicinal chemistry targets for the development of antitrypanosomatid and antimalarial therapeutics. Mol Biochem Parasitol (2003) 1.61

A crescent-shaped ALIX dimer targets ESCRT-III CHMP4 filaments. Structure (2009) 1.61

Structural insights into eRF3 and stop codon recognition by eRF1. Genes Dev (2009) 1.57

Structural analysis of intrinsically disordered proteins by small-angle X-ray scattering. Mol Biosyst (2011) 1.56

A helical structural nucleus is the primary elongating unit of insulin amyloid fibrils. PLoS Biol (2007) 1.56

Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly. Nat Commun (2010) 1.56

Binding of Clostridium difficile surface layer proteins to gastrointestinal tissues. Infect Immun (2002) 1.55

Addition of missing loops and domains to protein models by x-ray solution scattering. Biophys J (2002) 1.54

Structures of ABCB10, a human ATP-binding cassette transporter in apo- and nucleotide-bound states. Proc Natl Acad Sci U S A (2013) 1.54

The SAXS solution structure of RF1 differs from its crystal structure and is similar to its ribosome bound cryo-EM structure. Mol Cell (2005) 1.53

Absence of equilibrium cluster phase in concentrated lysozyme solutions. Proc Natl Acad Sci U S A (2008) 1.50

Clostridium difficile has two parallel and essential Sec secretion systems. J Biol Chem (2011) 1.50

Rac1 and PAK1 are upstream of IKK-epsilon and TBK-1 in the viral activation of interferon regulatory factor-3. FEBS Lett (2004) 1.47

Phosphatidylinositol 3-kinase inhibition broadly sensitizes glioblastoma cells to death receptor- and drug-induced apoptosis. Cancer Res (2008) 1.46

Common architecture of nuclear receptor heterodimers on DNA direct repeat elements with different spacings. Nat Struct Mol Biol (2011) 1.46

The structural basis of ZMPSTE24-dependent laminopathies. Science (2013) 1.43

Interaction of human complement with Sbi, a staphylococcal immunoglobulin-binding protein: indications of a novel mechanism of complement evasion by Staphylococcus aureus. J Biol Chem (2008) 1.42

Characterization of the cleavage site and function of resulting cleavage fragments after limited proteolysis of Clostridium difficile toxin B (TcdB) by host cells. Microbiology (2005) 1.40

Conformational space of flexible biological macromolecules from average data. J Am Chem Soc (2010) 1.39

Phagocytosis, an alternative model system for the study of cell adhesion. Semin Cell Dev Biol (2004) 1.39

Report of the wwPDB Small-Angle Scattering Task Force: data requirements for biomolecular modeling and the PDB. Structure (2013) 1.39

ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein kinase. Cell Metab (2011) 1.37

Acceptability of an internet-based partner notification system for sexually transmitted infection exposure among men who have sex with men. Am J Public Health (2007) 1.37

Small-angle X-ray scattering on biological macromolecules and nanocomposites in solution. Annu Rev Phys Chem (2012) 1.36

Structure and RNA interactions of the N-terminal RRM domains of PTB. Structure (2004) 1.35

Distinctive profiles of infection and pathology in hamsters infected with Clostridium difficile strains 630 and B1. Infect Immun (2009) 1.35

Crystal structure of Mycobacterium tuberculosis catalase-peroxidase. J Biol Chem (2004) 1.34

The structural basis of signal transduction for the response regulator PrrA from Mycobacterium tuberculosis. J Biol Chem (2006) 1.34

N-myristoyltransferase: a prospective drug target for protozoan parasites. ChemMedChem (2008) 1.33

Gonococcal, chlamydia, and syphilis infection positivity among MSM attending a large primary care clinic, Boston, 2003 to 2004. Sex Transm Dis (2009) 1.32

Small-molecule XIAP inhibitors enhance gamma-irradiation-induced apoptosis in glioblastoma. Neoplasia (2009) 1.31

Atomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin. J Biol Chem (2009) 1.31

A novel genetic switch controls phase variable expression of CwpV, a Clostridium difficile cell wall protein. Mol Microbiol (2009) 1.30

Conformation of polypyrimidine tract binding protein in solution. Structure (2006) 1.30

Characterization and selective inhibition of myristoyl-CoA:protein N-myristoyltransferase from Trypanosoma brucei and Leishmania major. Biochem J (2006) 1.30

Low-resolution structure of a vesicle disrupting α-synuclein oligomer that accumulates during fibrillation. Proc Natl Acad Sci U S A (2011) 1.29

Revised nomenclature of Clostridium difficile toxins and associated genes. J Med Microbiol (2005) 1.28

A different conformation for EGC stator subcomplex in solution and in the assembled yeast V-ATPase: possible implications for regulatory disassembly. Structure (2008) 1.28

A naturally derived outer-membrane vesicle vaccine protects against lethal pulmonary Burkholderia pseudomallei infection. Vaccine (2011) 1.27

X-ray and neutron small-angle scattering analysis of the complex formed by the Met receptor and the Listeria monocytogenes invasion protein InlB. J Mol Biol (2008) 1.27

Structural characterization of protein-protein complexes by integrating computational docking with small-angle scattering data. J Mol Biol (2010) 1.26

Immunization with Bacillus spores expressing toxin A peptide repeats protects against infection with Clostridium difficile strains producing toxins A and B. Infect Immun (2011) 1.24

Salmonella vaccines for use in humans: present and future perspectives. FEMS Microbiol Rev (2002) 1.22

Atomic resolution insight into host cell recognition by Toxoplasma gondii. EMBO J (2007) 1.22

Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus. EMBO J (2005) 1.21

Applications of small-angle X-ray scattering to biomacromolecular solutions. Int J Biochem Cell Biol (2012) 1.20

Domain conformation of tau protein studied by solution small-angle X-ray scattering. Biochemistry (2008) 1.20

Microarray analysis of the transcriptional responses of Clostridium difficile to environmental and antibiotic stress. J Med Microbiol (2008) 1.20

Joint use of small-angle X-ray and neutron scattering to study biological macromolecules in solution. Eur Biophys J (2006) 1.20