Published in Biochemistry on June 22, 1999
Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. Nature (2015) 2.43
Internal hydration increases during activation of the G-protein-coupled receptor rhodopsin. J Mol Biol (2008) 1.58
NMR spectroscopy in studies of light-induced structural changes in mammalian rhodopsin: applicability of solution (19)F NMR. Proc Natl Acad Sci U S A (1999) 1.43
G protein-coupled receptor drug discovery: implications from the crystal structure of rhodopsin. Curr Opin Drug Discov Devel (2001) 1.33
Solution 19F nuclear Overhauser effects in structural studies of the cytoplasmic domain of mammalian rhodopsin. Proc Natl Acad Sci U S A (2001) 1.27
Mechanism of signal propagation upon retinal isomerization: insights from molecular dynamics simulations of rhodopsin restrained by normal modes. Biophys J (2008) 1.18
Structure and function in rhodopsin: Mass spectrometric identification of the abnormal intradiscal disulfide bond in misfolded retinitis pigmentosa mutants. Proc Natl Acad Sci U S A (2001) 1.15
What site-directed labeling studies tell us about the mechanism of rhodopsin activation and G-protein binding. Photochem Photobiol Sci (2010) 0.90
Characterization of membrane protein non-native states. 2. The SDS-unfolded states of rhodopsin. Biochemistry (2010) 0.88
Structural states and dynamics of the D-loop in actin. Biophys J (2012) 0.87
A minimal ligand binding pocket within a network of correlated mutations identified by multiple sequence and structural analysis of G protein coupled receptors. BMC Biophys (2012) 0.79
Identification of motions in membrane proteins by elastic network models and their experimental validation. Methods Mol Biol (2012) 0.79
Characterization of membrane protein non-native states. 1. Extent of unfolding and aggregation of rhodopsin in the presence of chemical denaturants. Biochemistry (2010) 0.79
The roles of transmembrane domain helix-III during rhodopsin photoactivation. PLoS One (2011) 0.78
Differential dynamics of extracellular and cytoplasmic domains in denatured States of rhodopsin. Biochemistry (2014) 0.77
Alcohol binding in the C1 (C1A+C1B) domain of protein kinase C epsilon. Biochim Biophys Acta (2015) 0.76
CV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxyribonucleotide corresponding to the nucleotide sequence 31 to 50. J Mol Biol (1972) 58.52
Reversal of bacteriophage T4 induced polynucleotide kinase action. Biochemistry (1973) 21.41
Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry (1973) 11.45
Molecular motion in spin-labeled phospholipids and membranes. J Am Chem Soc (1971) 7.23
Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin. Science (1996) 6.50
High-pressure liquid chromatography in polynucleotide synthesis. Biochemistry (1978) 5.98
Identifying conformational changes with site-directed spin labeling. Nat Struct Biol (2000) 5.76
Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics. Biochemistry (1996) 5.52
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells. Proc Natl Acad Sci U S A (1987) 5.11
Vibrational spectroscopy of bacteriorhodopsin mutants: light-driven proton transport involves protonation changes of aspartic acid residues 85, 96, and 212. Biochemistry (1988) 4.93
Spin-label studies of the excitable membranes of nerve and muscle. Proc Natl Acad Sci U S A (1968) 4.58
Glutamic acid-113 serves as the retinylidene Schiff base counterion in bovine rhodopsin. Proc Natl Acad Sci U S A (1989) 4.44
A collision gradient method to determine the immersion depth of nitroxides in lipid bilayers: application to spin-labeled mutants of bacteriorhodopsin. Proc Natl Acad Sci U S A (1994) 4.27
Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants. Science (1990) 4.07
Studies on polynucleotides. LI. Syntheses of the 64 possible ribotrinucleotides derived from the four major ribomononucleotides. J Am Chem Soc (1966) 3.83
Amino acid sequence of bacteriorhodopsin. Proc Natl Acad Sci U S A (1979) 3.74
Studies on polynucleotides, XLIX. Stimulation of the binding of aminoacyl-sRNA's to ribosomes by ribotrinucleotides and a survey of codon assignments for 20 amino acids. Proc Natl Acad Sci U S A (1965) 3.71
Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons. Proc Natl Acad Sci U S A (1984) 3.67
The membrane dipole potential in a total membrane potential model. Applications to hydrophobic ion interactions with membranes. Biophys J (1986) 3.51
Crystal structures of spin labeled T4 lysozyme mutants: implications for the interpretation of EPR spectra in terms of structure. Biochemistry (2000) 3.46
Molecular motion of spin labeled side chains in alpha-helices: analysis by variation of side chain structure. Biochemistry (2001) 3.45
The bacteriorhodopsin gene. Proc Natl Acad Sci U S A (1981) 3.40
Electrostatic interactions in membranes and proteins. Annu Rev Biophys Biophys Chem (1986) 3.28
Structural studies on transmembrane proteins. 2. Spin labeling of bacteriorhodopsin mutants at unique cysteines. Biochemistry (1989) 3.25
Aspartic acid substitutions affect proton translocation by bacteriorhodopsin. Proc Natl Acad Sci U S A (1988) 3.13
Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase. Proc Natl Acad Sci U S A (1970) 3.11
Watching proteins move using site-directed spin labeling. Structure (1996) 3.09
Total synthesis of a gene for bovine rhodopsin. Proc Natl Acad Sci U S A (1986) 3.08
Aspartic acid-96 is the internal proton donor in the reprotonation of the Schiff base of bacteriorhodopsin. Proc Natl Acad Sci U S A (1989) 3.06
Cysteine residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin. Proc Natl Acad Sci U S A (1988) 3.01
Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations. Biochemistry (2001) 2.89
Structure and function in rhodopsin. 7. Point mutations associated with autosomal dominant retinitis pigmentosa. Biochemistry (1994) 2.88
Substitution of amino acids Asp-85, Asp-212, and Arg-82 in bacteriorhodopsin affects the proton release phase of the pump and the pK of the Schiff base. Proc Natl Acad Sci U S A (1990) 2.80
Bacterio-opsin mRNA in wild-type and bacterio-opsin-deficient Halobacterium halobium strains. Proc Natl Acad Sci U S A (1984) 2.77
A transposable element from Halobacterium halobium which inactivates the bacteriorhodopsin gene. Proc Natl Acad Sci U S A (1982) 2.76
High-frequency spontaneous mutation in the bacterio-opsin gene in Halobacterium halobium is mediated by transposable elements. Proc Natl Acad Sci U S A (1983) 2.70
Replacement of aspartic acid-96 by asparagine in bacteriorhodopsin slows both the decay of the M intermediate and the associated proton movement. Proc Natl Acad Sci U S A (1989) 2.64
Total synthesis of the gene for an alanine transfer ribonucleic acid from yeast. Nature (1970) 2.51
Visual transduction in vertebrate photoreceptors. Annu Rev Neurosci (1979) 2.45
Studies on polynucleotides, lxviii the primary structure of yeast phenylalanine transfer RNA. Proc Natl Acad Sci U S A (1967) 2.45
Preparation and properties of phospholipid bilayers containing rhodopsin. Proc Natl Acad Sci U S A (1972) 2.42
Total synthesis of a gene. Science (1979) 2.38
A single amino acid substitution in rhodopsin (lysine 248----leucine) prevents activation of transducin. J Biol Chem (1988) 2.37
CIV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxynucleotide corresponding to the nucleotide sequence 21 to 40. J Mol Biol (1972) 2.35
Structure and function in rhodopsin. Measurement of the rate of metarhodopsin II decay by fluorescence spectroscopy. J Biol Chem (1995) 2.34
Orientation and motion of amphiphilic spin labels in membranes. Proc Natl Acad Sci U S A (1969) 2.29
Molecular characterization of helix-loop-helix peptides. Science (1992) 2.26
Structure and function in rhodopsin: high level expression of a synthetic bovine opsin gene and its mutants in stable mammalian cell lines. Proc Natl Acad Sci U S A (1996) 2.24
Orientation of bacteriorhodopsin in Halobacterium halobium as studied by selective proteolysis. Proc Natl Acad Sci U S A (1977) 2.23
Assembly of functional rhodopsin requires a disulfide bond between cysteine residues 110 and 187. J Biol Chem (1990) 2.18
The nucleotide sequence in the promoter region of the fene for an Escherichia coli tyrosine transfer ribonucleic acid. J Biol Chem (1975) 2.17
Partial primary structure of bacteriorhodopsin: sequencing methods for membrane proteins. Proc Natl Acad Sci U S A (1979) 2.17
Structure and function in rhodopsin: the role of asparagine-linked glycosylation. Proc Natl Acad Sci U S A (1994) 2.17
Rhodopsin mutants that bind but fail to activate transducin. Science (1990) 2.16
GTPase of bovine rod outer segments: the amino acid sequence of the alpha subunit as derived from the cDNA sequence. Proc Natl Acad Sci U S A (1985) 2.09
Studies on polynucleotides. 118. A further study of ribonucleotide incorporation into deoxyribonucleic acid chains by deoxyribonucleic acid polymerase I of Escherichia coli. J Biol Chem (1972) 2.09
Glycerophospholipid synthesis: improved general method and new analogs containing photoactivable groups. Proc Natl Acad Sci U S A (1977) 2.07
Studies on polynucleotides. LXIX. Synthetic deoxyribopolynucleotides as templates for the DNA polymerase of Escherichia coli: DNA-like polymers containing repeating trinucleotide sequences. J Mol Biol (1967) 2.06
Replacement of aspartic residues 85, 96, 115, or 212 affects the quantum yield and kinetics of proton release and uptake by bacteriorhodopsin. Proc Natl Acad Sci U S A (1989) 2.04
Refolding of an integral membrane protein. Denaturation, renaturation, and reconstitution of intact bacteriorhodopsin and two proteolytic fragments. J Biol Chem (1981) 2.03
Studies on polynucleotides. LXVII. Initiation of protein synthesis in vitro as studied by using ribopolynucleotides with repeating nucleotide sequences as messengers. J Mol Biol (1967) 2.02
Nucleotide sequence in the promoter region of the Escherichia coli tyrosine tRNA gene. Proc Natl Acad Sci U S A (1974) 2.01
Role of the intradiscal domain in rhodopsin assembly and function. Proc Natl Acad Sci U S A (1990) 1.97
Spin labeled cysteines as sensors for protein-lipid interaction and conformation in rhodopsin. Photochem Photobiol (1992) 1.96
Protonation state of Asp (Glu)-85 regulates the purple-to-blue transition in bacteriorhodopsin mutants Arg-82----Ala and Asp-85----Glu: the blue form is inactive in proton translocation. Proc Natl Acad Sci U S A (1990) 1.94
Motion of fatty acid spin labels in the plasma membrane of mycoplasma. Biochim Biophys Acta (1970) 1.91
Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 1. General introduction. J Biol Chem (1976) 1.91
alpha 1-adrenergic receptor subtypes. Molecular structure, function, and signaling. Circ Res (1996) 1.90
Studies on polynucleotides. 103. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. J Mol Biol (1972) 1.90
Temperature- and light-dependent structural changes in rhodopsin-lipid membranes. Exp Eye Res (1973) 1.89
Lipid requirements for Rhodopsin regenerability. Biochemistry (1973) 1.89
Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: a site-directed spin-labeling study. Biochemistry (1996) 1.88
Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA's as catalyzed by DNA polymerases. J Mol Biol (1971) 1.87
Site of attachment of retinal in bacteriorhodopsin. Proc Natl Acad Sci U S A (1981) 1.87
Structure of the lipopolysaccharide from an Escherichia coli heptose-less mutant. I. Chemical degradations and identification of products. J Biol Chem (1979) 1.86
Structure and function in rhodopsin. Studies of the interaction between the rhodopsin cytoplasmic domain and transducin. J Biol Chem (1992) 1.82
Mapping of the amino acids in membrane-embedded helices that interact with the retinal chromophore in bovine rhodopsin. J Biol Chem (1991) 1.80
Stability of "salt bridges" in membrane proteins. Proc Natl Acad Sci U S A (1984) 1.79
Rapid long-range proton diffusion along the surface of the purple membrane and delayed proton transfer into the bulk. Proc Natl Acad Sci U S A (1995) 1.77
Conformation of T4 lysozyme in solution. Hinge-bending motion and the substrate-induced conformational transition studied by site-directed spin labeling. Biochemistry (1997) 1.76
Colicin E1 binding to membranes: time-resolved studies of spin-labeled mutants. Science (1993) 1.73
Structure-function studies on bacteriorhodopsin. X. Individual substitutions of arginine residues by glutamine affect chromophore formation, photocycle, and proton translocation. J Biol Chem (1989) 1.73
Studies on polynucleotides. LXXXVII. The joining of short deoxyribopolynucleotides by DNA-joining enzymes. Proc Natl Acad Sci U S A (1968) 1.72
Polynucleotide ligase-catalyzed joining of deoxyribo-oligonucleotides on ribopolynucleotide templates and of ribo-oligonucleotides on deoxyribopolynucleotide templates. Proc Natl Acad Sci U S A (1970) 1.70
Time-resolved detection of structural changes during the photocycle of spin-labeled bacteriorhodopsin. Science (1994) 1.70
Replacement of leucine-93 by alanine or threonine slows down the decay of the N and O intermediates in the photocycle of bacteriorhodopsin: implications for proton uptake and 13-cis-retinal----all-trans-retinal reisomerization. Proc Natl Acad Sci U S A (1991) 1.69
Site-directed isotope labelling and FTIR spectroscopy of bacteriorhodopsin. Nat Struct Biol (1994) 1.67
Site-specific incorporation of biophysical probes into proteins. Proc Natl Acad Sci U S A (1994) 1.66
A further study of misreading of codons induced by streptomycin and neomycin using ribopolynucleotides containing two nucleotides in alternating sequence as templates. J Mol Biol (1966) 1.65