Creation of human tumour cells with defined genetic elements.

PubWeight™: 16.47‹?› | Rank: Top 0.1% | All-Time Top 10000

🔗 View Article (PMID 10440377)

Published in Nature on July 29, 1999

Authors

W C Hahn1, C M Counter, A S Lundberg, R L Beijersbergen, M W Brooks, R A Weinberg

Author Affiliations

1: Department of Biology, Massachusetts Institute of Technology, Whitehead Institute for Biomedical Research, Cambridge 02142, USA.

Associated clinical trials:

Telomere Associated Variables (TAVs) in Prostate Cancer | NCT04124900

Articles citing this

(truncated to the top 100)

The epithelial-mesenchymal transition generates cells with properties of stem cells. Cell (2008) 46.08

Human induced pluripotent stem cells free of vector and transgene sequences. Science (2009) 17.16

Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells. Genes Dev (2001) 11.25

Identification of a novel polyomavirus from patients with acute respiratory tract infections. PLoS Pathog (2007) 8.01

Ras oncogenes: split personalities. Nat Rev Mol Cell Biol (2008) 7.91

Selective killing of cancer cells by a small molecule targeting the stress response to ROS. Nature (2011) 7.03

Identification of a cell of origin for human prostate cancer. Science (2010) 6.33

Oncogenic transformation by inhibitor-sensitive and -resistant EGFR mutants. PLoS Med (2005) 6.04

Telomere diseases. N Engl J Med (2009) 5.68

Human keratinocytes that express hTERT and also bypass a p16(INK4a)-enforced mechanism that limits life span become immortal yet retain normal growth and differentiation characteristics. Mol Cell Biol (2000) 5.28

A genome-wide association study identifies pancreatic cancer susceptibility loci on chromosomes 13q22.1, 1q32.1 and 5p15.33. Nat Genet (2010) 4.89

Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Mol Cell Biol (2002) 4.77

The melanocyte differentiation program predisposes to metastasis after neoplastic transformation. Nat Genet (2005) 4.70

Distinct requirements for Ras oncogenesis in human versus mouse cells. Genes Dev (2002) 4.30

Telomeres and telomerase in cancer. Carcinogenesis (2009) 4.28

Multiple mutations and cancer. Proc Natl Acad Sci U S A (2003) 4.21

Immortal human pancreatic duct epithelial cell lines with near normal genotype and phenotype. Am J Pathol (2000) 4.19

Normal and leukemic hematopoiesis: are leukemias a stem cell disorder or a reacquisition of stem cell characteristics? Proc Natl Acad Sci U S A (2003) 4.09

A transcriptional signature and common gene networks link cancer with lipid metabolism and diverse human diseases. Cancer Cell (2010) 4.02

Systemic endocrine instigation of indolent tumor growth requires osteopontin. Cell (2008) 3.95

Microenvironment determines lineage fate in a human model of MLL-AF9 leukemia. Cancer Cell (2008) 3.79

Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Cell (2008) 3.75

PML is induced by oncogenic ras and promotes premature senescence. Genes Dev (2000) 3.35

RAS-RAF-MEK-dependent oxidative cell death involving voltage-dependent anion channels. Nature (2007) 3.21

Perturbational profiling of a cell-line model of tumorigenesis by using metabolic measurements. Proc Natl Acad Sci U S A (2005) 3.18

Human telomerase and its regulation. Microbiol Mol Biol Rev (2002) 3.11

KRAS and YAP1 converge to regulate EMT and tumor survival. Cell (2014) 3.06

Telomeres, stem cells, senescence, and cancer. J Clin Invest (2004) 2.87

Increased epidermal tumors and increased skin wound healing in transgenic mice overexpressing the catalytic subunit of telomerase, mTERT, in basal keratinocytes. EMBO J (2001) 2.83

Telomerase contributes to tumorigenesis by a telomere length-independent mechanism. Proc Natl Acad Sci U S A (2002) 2.82

Modeling high-grade serous ovarian carcinogenesis from the fallopian tube. Proc Natl Acad Sci U S A (2011) 2.71

Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence. Mol Cell Biol (2002) 2.70

Interplay between oncogene-induced DNA damage response and heterochromatin in senescence and cancer. Nat Cell Biol (2011) 2.69

Why do viruses cause cancer? Highlights of the first century of human tumour virology. Nat Rev Cancer (2010) 2.68

A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence mechanism that limits replicative potential independent of telomere status. Mol Cell Biol (2002) 2.66

What has senescence got to do with cancer? Cancer Cell (2005) 2.55

Stem cell chromatin patterns: an instructive mechanism for DNA hypermethylation? Cell Cycle (2007) 2.55

Increased Wnt signaling triggers oncogenic conversion of human breast epithelial cells by a Notch-dependent mechanism. Proc Natl Acad Sci U S A (2006) 2.49

Telomere-based crisis: functional differences between telomerase activation and ALT in tumor progression. Genes Dev (2003) 2.38

Human Merkel cell polyomavirus small T antigen is an oncoprotein targeting the 4E-BP1 translation regulator. J Clin Invest (2011) 2.37

Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death. Proc Natl Acad Sci U S A (1999) 2.36

Cross-platform analysis of cancer microarray data improves gene expression based classification of phenotypes. BMC Bioinformatics (2005) 2.36

Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production. Proc Natl Acad Sci U S A (2007) 2.34

Refractory nature of normal human diploid fibroblasts with respect to oncogene-mediated transformation. Proc Natl Acad Sci U S A (2003) 2.25

The cytoplasmic deacetylase HDAC6 is required for efficient oncogenic tumorigenesis. Cancer Res (2008) 2.17

N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo. Mol Cell Biol (2001) 2.16

Telomerase RNA accumulates in Cajal bodies in human cancer cells. Mol Biol Cell (2003) 2.14

MTH1 inhibition eradicates cancer by preventing sanitation of the dNTP pool. Nature (2014) 2.11

Synthetic lethal screening identifies compounds activating iron-dependent, nonapoptotic cell death in oncogenic-RAS-harboring cancer cells. Chem Biol (2008) 2.04

RALA and RALBP1 regulate mitochondrial fission at mitosis. Nat Cell Biol (2011) 2.03

Constitutive telomerase expression promotes mammary carcinomas in aging mice. Proc Natl Acad Sci U S A (2002) 2.03

Frequency of K-ras mutations in pancreatic intraductal neoplasias associated with pancreatic ductal adenocarcinoma and chronic pancreatitis: a meta-analysis. Neoplasia (2005) 2.03

Omeprazole blocks eotaxin-3 expression by oesophageal squamous cells from patients with eosinophilic oesophagitis and GORD. Gut (2012) 2.00

Disruption of the telomerase catalytic subunit gene from Arabidopsis inactivates telomerase and leads to a slow loss of telomeric DNA. Proc Natl Acad Sci U S A (1999) 1.99

The Saccharomyces cerevisiae WRN homolog Sgs1p participates in telomere maintenance in cells lacking telomerase. EMBO J (2001) 1.99

Id1 regulation of cellular senescence through transcriptional repression of p16/Ink4a. Proc Natl Acad Sci U S A (2001) 1.98

Natural and pharmacological regulation of telomerase. Nucleic Acids Res (2002) 1.92

T antigens of simian virus 40: molecular chaperones for viral replication and tumorigenesis. Microbiol Mol Biol Rev (2002) 1.92

Constitutively activated Stat3 protects fibroblasts from serum withdrawal and UV-induced apoptosis and antagonizes the proapoptotic effects of activated Stat1. Proc Natl Acad Sci U S A (2001) 1.89

Ovarian cancer spheroids use myosin-generated force to clear the mesothelium. Cancer Discov (2011) 1.88

Catabolic efficiency of aerobic glycolysis: the Warburg effect revisited. BMC Syst Biol (2010) 1.88

Conditional immortalization of freshly isolated human mammary fibroblasts and endothelial cells. Proc Natl Acad Sci U S A (2001) 1.87

ATARiS: computational quantification of gene suppression phenotypes from multisample RNAi screens. Genome Res (2012) 1.86

Expression of the telomerase catalytic subunit, hTERT, induces resistance to transforming growth factor beta growth inhibition in p16INK4A(-) human mammary epithelial cells. Proc Natl Acad Sci U S A (2001) 1.83

Telomerase reverse transcriptase promotes cardiac muscle cell proliferation, hypertrophy, and survival. Proc Natl Acad Sci U S A (2001) 1.83

Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1. Cell (2014) 1.82

TERT promoter mutations in bladder cancer affect patient survival and disease recurrence through modification by a common polymorphism. Proc Natl Acad Sci U S A (2013) 1.82

DYRK1A protein kinase promotes quiescence and senescence through DREAM complex assembly. Genes Dev (2011) 1.82

Transformation of human and murine fibroblasts without viral oncoproteins. Mol Cell Biol (2005) 1.81

Downregulation of 14-3-3sigma prevents clonal evolution and leads to immortalization of primary human keratinocytes. J Cell Biol (2000) 1.81

Regulation of the human catalytic subunit of telomerase (hTERT). Gene (2012) 1.81

DACH1 is a cell fate determination factor that inhibits cyclin D1 and breast tumor growth. Mol Cell Biol (2006) 1.80

Oncogenic ras activates the ARF-p53 pathway to suppress epithelial cell transformation. Proc Natl Acad Sci U S A (2001) 1.79

A highly selective telomerase inhibitor limiting human cancer cell proliferation. EMBO J (2001) 1.79

BRD7 is a candidate tumour suppressor gene required for p53 function. Nat Cell Biol (2010) 1.76

Telomerase reactivation following telomere dysfunction yields murine prostate tumors with bone metastases. Cell (2012) 1.75

Rare codons regulate KRas oncogenesis. Curr Biol (2012) 1.74

Quantitation of telomerase components and hTERT mRNA splicing patterns in immortal human cells. Nucleic Acids Res (2001) 1.73

Oncogene-induced telomere dysfunction enforces cellular senescence in human cancer precursor lesions. EMBO J (2012) 1.71

DNA replication fidelity and cancer. Semin Cancer Biol (2010) 1.69

Structure-based design of selective and potent G quadruplex-mediated telomerase inhibitors. Proc Natl Acad Sci U S A (2001) 1.69

Human T-cell leukemia virus type 1 (HTLV-1) and leukemic transformation: viral infectivity, Tax, HBZ and therapy. Oncogene (2010) 1.67

PEX19 is a predominantly cytosolic chaperone and import receptor for class 1 peroxisomal membrane proteins. J Cell Biol (2004) 1.67

Sp1 is involved in Akt-mediated induction of VEGF expression through an HIF-1-independent mechanism. Mol Biol Cell (2004) 1.67

Replacement of normal with mutant alleles in the genome of normal human cells unveils mutation-specific drug responses. Proc Natl Acad Sci U S A (2008) 1.66

The cancer stem cell niche: how essential is the niche in regulating stemness of tumor cells? Cell Stem Cell (2015) 1.66

Genetic reconstruction of individual colorectal tumor histories. Proc Natl Acad Sci U S A (2000) 1.63

Resistance to chemotherapy: new treatments and novel insights into an old problem. Br J Cancer (2008) 1.62

Definition of pRB- and p53-dependent and -independent steps in HIRA/ASF1a-mediated formation of senescence-associated heterochromatin foci. Mol Cell Biol (2007) 1.62

In vitro generation of human cells with cancer stem cell properties. Nat Cell Biol (2011) 1.61

Dysregulation of ribosome biogenesis and translational capacity is associated with tumor progression of human breast cancer cells. PLoS One (2009) 1.60

Bmi-1 is essential for the tumorigenicity of neuroblastoma cells. Am J Pathol (2007) 1.59

E box-dependent activation of telomerase by human papillomavirus type 16 E6 does not require induction of c-myc. J Virol (2001) 1.58

A systematic search for downstream mediators of tumor suppressor function of p53 reveals a major role of BTG2 in suppression of Ras-induced transformation. Genes Dev (2006) 1.58

Kaposi's sarcoma-associated herpesvirus confers a survival advantage to endothelial cells. Cancer Res (2008) 1.57

Pretumor progression: clonal evolution of human stem cell populations. Am J Pathol (2004) 1.57

Human lung epithelial cells progressed to malignancy through specific oncogenic manipulations. Mol Cancer Res (2013) 1.57

Aneuploidy vs. gene mutation hypothesis of cancer: recent study claims mutation but is found to support aneuploidy. Proc Natl Acad Sci U S A (2000) 1.54

Regulation of G1 Cell Cycle Progression: Distinguishing the Restriction Point from a Nutrient-Sensing Cell Growth Checkpoint(s). Genes Cancer (2010) 1.53

Simultaneous tyrosine and serine phosphorylation of STAT3 transcription factor is involved in Rho A GTPase oncogenic transformation. Mol Biol Cell (2001) 1.53

Articles by these authors

The hallmarks of cancer. Cell (2000) 113.05

Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature (1983) 19.76

Tumor spectrum analysis in p53-mutant mice. Curr Biol (1994) 16.60

hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Cell (2001) 15.97

A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature (1986) 13.96

Mechanism of activation of a human oncogene. Nature (1982) 12.09

Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells. Genes Dev (2001) 11.25

Effects of an Rb mutation in the mouse. Nature (1992) 11.06

Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. Nature (1988) 10.98

hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell (1997) 8.74

Regulation of retinoblastoma protein functions by ectopic expression of human cyclins. Cell (1992) 7.61

Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation. Nature (1993) 7.20

Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation. Nature (1985) 7.07

Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Cell (1986) 7.06

Multiple protein-binding sites in the 5'-flanking region regulate c-fos expression. Mol Cell Biol (1986) 7.06

Isolation of a transforming sequence from a human bladder carcinoma cell line. Cell (1982) 6.93

Cellular oncogenes and multistep carcinogenesis. Science (1983) 6.52

The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature (1985) 6.23

Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene. Nature (1982) 6.09

Cyclin D1 provides a link between development and oncogenesis in the retina and breast. Cell (1995) 6.08

The integrated genome of murine leukemia virus. Cell (1978) 5.84

The neu oncogene encodes an epidermal growth factor receptor-related protein. Nature (1986) 5.77

Passage of phenotypes of chemically transformed cells via transfection of DNA and chromatin. Proc Natl Acad Sci U S A (1979) 5.32

Physical interaction of the retinoblastoma protein with human D cyclins. Cell (1993) 5.31

Human keratinocytes that express hTERT and also bypass a p16(INK4a)-enforced mechanism that limits life span become immortal yet retain normal growth and differentiation characteristics. Mol Cell Biol (2000) 5.28

Acrylamide gel electrophoresis of HeLa cell nucleolar RNA. Proc Natl Acad Sci U S A (1967) 5.06

Small molecular weight monodisperse nuclear RNA. J Mol Biol (1968) 4.96

Inhibition of telomerase limits the growth of human cancer cells. Nat Med (1999) 4.96

Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature (1981) 4.72

Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase. Cell (1992) 4.68

Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes. Mol Cell Biol (1998) 4.54

DNA sequences homologous to vertebrate oncogenes are conserved in Drosophila melanogaster. Proc Natl Acad Sci U S A (1981) 4.17

Suppression of intestinal neoplasia by DNA hypomethylation. Cell (1995) 4.16

Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc Natl Acad Sci U S A (1998) 4.11

Oncogenic activity of the c-Myc protein requires dimerization with Max. Cell (1993) 4.10

Isolation and characterization of simian virus 40 ribonucleic acid. J Virol (1972) 4.09

Frequent inactivation of the retinoblastoma anti-oncogene is restricted to a subset of human tumor cells. Proc Natl Acad Sci U S A (1990) 4.06

Cyclin D2 is an FSH-responsive gene involved in gonadal cell proliferation and oncogenesis. Nature (1996) 4.05

Restriction endonuclease cleavage of linear and closed circular murine leukemia viral DNAs: discovery of a smaller circular form. Cell (1979) 4.04

Tumour predisposition in mice heterozygous for a targeted mutation in Nf1. Nat Genet (1994) 3.97

A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Cell (1979) 3.74

Deletions of a DNA sequence in retinoblastomas and mesenchymal tumors: organization of the sequence and its encoded protein. Proc Natl Acad Sci U S A (1987) 3.72

Identification of DNA fragments carrying ecotropic proviruses of AKR mice. Proc Natl Acad Sci U S A (1979) 3.64

The action of oncogenes in the cytoplasm and nucleus. Science (1985) 3.62

Shared role of the pRB-related p130 and p107 proteins in limb development. Genes Dev (1996) 3.61

Phenotype of mice lacking functional Deleted in colorectal cancer (Dcc) gene. Nature (1997) 3.57

Heterotypic signaling between epithelial tumor cells and fibroblasts in carcinoma formation. Exp Cell Res (2001) 3.48

Transcriptional activation by the human c-Myc oncoprotein in yeast requires interaction with Max. Nature (1992) 3.43

Cell cycle-specific association of E2F with the p130 E1A-binding protein. Genes Dev (1993) 3.41

Point mutational inactivation of the retinoblastoma antioncogene. Science (1989) 3.37

Essential function of Wnt-4 in mammary gland development downstream of progesterone signaling. Genes Dev (2000) 3.30

Characterization of a human colon/lung carcinoma oncogene. Nature (1983) 3.26

A paracrine role for the epithelial progesterone receptor in mammary gland development. Proc Natl Acad Sci U S A (1998) 3.23

Proposal for naming host cell-derived inserts in retrovirus genomes. J Virol (1981) 3.18

Altered cell cycle kinetics, gene expression, and G1 restriction point regulation in Rb-deficient fibroblasts. Mol Cell Biol (1996) 3.15

Infectious viral DNA of murine leukemia virus. Proc Natl Acad Sci U S A (1975) 3.09

Growth suppression by p16ink4 requires functional retinoblastoma protein. Proc Natl Acad Sci U S A (1995) 3.08

Murine leukemia virus: detection of unintegrated double-stranded DNA forms of the provirus. Proc Natl Acad Sci U S A (1975) 3.07

The pathway to signal achievement. Nature (1993) 3.03

The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell (1983) 3.00

E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle. Proc Natl Acad Sci U S A (1995) 2.99

Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells. Proc Natl Acad Sci U S A (1997) 2.97

Structure of the intermediates leading to the integrated provirus. Biochim Biophys Acta (1977) 2.96

Regulation of cyclin E transcription by E2Fs and retinoblastoma protein. Oncogene (1996) 2.96

Association between GTPase activators for Rho and Ras families. Nature (1992) 2.92

Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus. Cell (1992) 2.90

Expression cloning and characterization of the TGF-beta type III receptor. Cell (1991) 2.89

G1/S phosphorylation of the retinoblastoma protein is associated with an altered affinity for the nuclear compartment. Cell (1991) 2.87

Function of a human cyclin gene as an oncogene. Proc Natl Acad Sci U S A (1994) 2.87

Association of Cdk-activating kinase subunits with transcription factor TFIIH. Nature (1995) 2.87

Behavior of myc and ras oncogenes in transformation of rat embryo fibroblasts. Mol Cell Biol (1986) 2.86

The neu gene: an erbB-homologous gene distinct from and unlinked to the gene encoding the EGF receptor. Science (1985) 2.80

Three different human tumor cell lines contain different oncogenes. Cell (1981) 2.77

Telomerase, cell immortality, and cancer. Cold Spring Harb Symp Quant Biol (1994) 2.76

Down-modulation of an oncogene protein product and reversion of the transformed phenotype by monoclonal antibodies. Cell (1985) 2.75

The catalytic subunit of yeast telomerase. Proc Natl Acad Sci U S A (1997) 2.72

Processing of 45 s nucleolar RNA. J Mol Biol (1970) 2.60

Oncogenic activation of the neu-encoded receptor protein by point mutation and deletion. EMBO J (1988) 2.59

Extensive contribution of Rb-deficient cells to adult chimeric mice with limited histopathological consequences. EMBO J (1994) 2.57

Expression of TERT in early premalignant lesions and a subset of cells in normal tissues. Nat Genet (1998) 2.56

Telomerase activity is restored in human cells by ectopic expression of hTERT (hEST2), the catalytic subunit of telomerase. Oncogene (1998) 2.56

Rescue of cyclin D1 deficiency by knockin cyclin E. Cell (1999) 2.54

Generation of novel, biologically active Harvey sarcoma viruses via apparent illegitimate recombination. J Virol (1981) 2.53

p185, a product of the neu proto-oncogene, is a receptorlike protein associated with tyrosine kinase activity. Mol Cell Biol (1986) 2.52

Adaptation of a retrovirus as a eucaryotic vector transmitting the herpes simplex virus thymidine kinase gene. Mol Cell Biol (1982) 2.51

"Frizzy" aggregation genes of the gliding bacterium Myxococcus xanthus show sequence similarities to the chemotaxis genes of enteric bacteria. Proc Natl Acad Sci U S A (1989) 2.49

Requirement for TFIIH kinase activity in transcription by RNA polymerase II. Nature (1995) 2.43

The telomere hypothesis of cellular aging. Exp Gerontol (1993) 2.39

Negative autoregulation of c-myc transcription. EMBO J (1990) 2.38

Nuclear RNA metabolism. Annu Rev Biochem (1973) 2.38

Identification and characterization of the mutL and mutS gene products of Salmonella typhimurium LT2. J Bacteriol (1985) 2.32

Increased tyrosine kinase activity associated with the protein encoded by the activated neu oncogene. Proc Natl Acad Sci U S A (1988) 2.31