Published in Biochemistry on December 01, 1968
Microtubule assembly in the absence of added nucleotides. Proc Natl Acad Sci U S A (1973) 18.46
A protein factor essential for microtubule assembly. Proc Natl Acad Sci U S A (1975) 15.35
Actin antibody: the specific visualization of actin filaments in non-muscle cells. Proc Natl Acad Sci U S A (1974) 11.64
Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3':5'-monophosphate and testosterone. Proc Natl Acad Sci U S A (1971) 6.12
Receptor mobility and receptor-cytoplasmic interactions in lymphocytes. Proc Natl Acad Sci U S A (1973) 5.13
Regulation of axon formation by clonal lines of a neural tumor. Proc Natl Acad Sci U S A (1970) 5.12
The slow component of axonal transport. Identification of major structural polypeptides of the axon and their generality among mammalian neurons. J Cell Biol (1975) 4.70
Axon growth: roles of microfilaments and microtubules. Proc Natl Acad Sci U S A (1970) 4.33
Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc Natl Acad Sci U S A (1970) 4.20
Mechanisms of lysosomal enzyme release from human leukocytes. II. Effects of cAMP and cGMP, autonomic agonists, and agents which affect microtubule function. J Clin Invest (1974) 3.65
Microtubules from mammalian brain: some properties of their depolymerization products and a proposed mechanism of assembly and disassembly. Proc Natl Acad Sci U S A (1974) 3.59
Are cytoplasmic microtubules heteropolymers? Proc Natl Acad Sci U S A (1971) 3.38
Complete amino acid sequence of beta-tubulin from porcine brain. Proc Natl Acad Sci U S A (1981) 3.24
Biochemistry of the filaments of brain. Proc Natl Acad Sci U S A (1976) 3.03
Precipitation of proteins by vinblastine and calcium ions. Proc Natl Acad Sci U S A (1970) 3.00
Effects of phagocytosis and colchicine on the distribution of lectin-binding sites on cell surfaces. Proc Natl Acad Sci U S A (1974) 2.93
Comparison of the microtubule proteins of neuroblastoma cells, brain, and Chlamydomonas flagella. Proc Natl Acad Sci U S A (1971) 2.68
Microtubule protein. Identification in and transport to nerve endings. J Cell Biol (1971) 2.58
Effect of colchicine and vinblastine on the topographical separation of membrane functions. J Exp Med (1972) 2.57
Cyclic adenosine 3':5'-monophosphate-stimulated phosphorylation of isolated neurotubule subunits. Proc Natl Acad Sci U S A (1970) 2.56
Rat brain microtubule protein: purification and determination of covalently bound phosphate and carbohydrate. Proc Natl Acad Sci U S A (1972) 2.52
Characteristics of the polar assembly and disassembly of microtubules observed in vitro by darkfield light microscopy. J Cell Biol (1979) 2.45
Mechanisms of lysosomal enzyme release from human leukocytes. I. Effect of cyclic nucleotides and colchicine. J Cell Biol (1973) 2.33
Structure of the DNA ligase-adenylate intermediate: lysine (epsilon-amino)-linked adenosine monophosphoramidate. Proc Natl Acad Sci U S A (1971) 2.29
Leukocytic proteases and the immunologic release of lysosomal enzymes. Am J Pathol (1972) 2.26
Potassium and amino acid transport in human leukocytes exposed to phagocytic stimuli. J Cell Biol (1974) 2.16
Isolation and partial characterization of alpha and beta-tubulin from outer doublets of sea-urchin sperm and microtubules of chick-embryo brain. Proc Natl Acad Sci U S A (1973) 2.14
Colchicine inhibition of nerve fiber formation in vitro. J Cell Biol (1972) 2.06
Possible role of microtubules in thyroid secretion. Proc Natl Acad Sci U S A (1970) 2.00
Polycation-induced assembly of purified tubulin. Proc Natl Acad Sci U S A (1976) 1.99
Synthesis and storage of microtubule proteins by sea urchin embryos. J Cell Biol (1971) 1.95
The effects of parathyroid hormone, colchicine, and calcitonin on the ultrastructure and the activity of osteoclasts in organ culture. J Cell Biol (1974) 1.94
Actin associated with membranes from 3T3 mouse fibroblast and HeLa cells. J Cell Biol (1975) 1.93
Colcemid inhibition of cell growth and the characterization of a colcemid-binding activity in Saccharomyces cerevisiae. J Cell Biol (1972) 1.89
Guanosinetriphosphatase activity of tubulin associated with microtubule assembly. Proc Natl Acad Sci U S A (1977) 1.85
The organization of synaptic axcplasm in the lamprey (petromyzon marinus) central nervous system. J Cell Biol (1970) 1.83
Rapid purification and the immunological specificity of mammalian microtubular paracrystals possessing an ATPase activity. Proc Natl Acad Sci U S A (1970) 1.80
Factors affecting the redistribution of surface-bound concanavalin A on human polymorphonuclear leukocytes. J Cell Biol (1974) 1.78
Heterogeneity of tubulin subunits. Proc Natl Acad Sci U S A (1971) 1.77
Rapid and Reversible High-Affinity Binding of the Dinitroaniline Herbicide Oryzalin to Tubulin from Zea mays L. Plant Physiol (1993) 1.75
A directed approach for engineering conditional protein stability using biologically silent small molecules. J Biol Chem (2007) 1.74
Aster formation in eggs of Xenopus laevis. Induction by isolated basal bodies. J Cell Biol (1975) 1.73
Drugs that target dynamic microtubules: a new molecular perspective. Med Res Rev (2011) 1.70
Direct biochemical measurements of microtubule assembly and disassembly in Chinese hamster ovary cells. The effect of intercellular contact, cold, D2O, and N6,O2'-dibutyryl cyclic adenosine monophosphate. J Cell Biol (1975) 1.69
The mechanism of action of colchicine. Colchicine binding properties of sea urchin sperm tail outer doublet tubulin. J Cell Biol (1973) 1.68
Modification of tubulin by tyrosylation in cells and extracts and its effect on assembly in vitro. J Cell Biol (1977) 1.67
Monoclonal antibodies that recognize discrete forms of tubulin. Proc Natl Acad Sci U S A (1982) 1.63
Changes in the organization of tubulin during meiosis in the eggs of the surf clam, Spisula solidissima. J Cell Biol (1972) 1.62
Characterization of the colchicine binding of membrane fractions from rat and mouse liver. J Cell Biol (1974) 1.62
Asymmetric behavior of severed microtubule ends after ultraviolet-microbeam irradiation of individual microtubules in vitro. J Cell Biol (1989) 1.59
A microtubule-associated protein in Drosophila melanogaster: identification, characterization, and isolation of coding sequences. J Cell Biol (1986) 1.59
Chromatographic and electrophoretic analysis of viral proteins from hamster and chicken cells transformed by Rous sarcoma virus. J Virol (1973) 1.49
Serological similarity of flagellar and mitotic microtubules. J Cell Biol (1971) 1.48
Fine structural changes in neurons and nerve fibers associated with colchicine inhibition of nerve fiber formation in vitro. J Cell Biol (1973) 1.45
The contractile and regulatory proteins of insect flight muscle. Biochem J (1973) 1.45
Identification of direct protein targets of small molecules. ACS Chem Biol (2010) 1.44
A correlation between the effects of anti-mitotic drugs on microtubule assembly in vitro and the inhibition of axonal transport in noradrenergic neurones. J Physiol (1975) 1.41
A role of Sep1 (= Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiae. EMBO J (1995) 1.40
Structure of microtubular crystals induced by vinblastine in vitro. J Cell Biol (1970) 1.39
In vitro aggregation of cytoplasmic microtubule subunits. Proc Natl Acad Sci U S A (1972) 1.36
Studies on the mechanism of action of nerve growth factor. Proc Natl Acad Sci U S A (1972) 1.32
A sensitive method for measuring polymerized and depolymerized forms of tubulin in tissues. J Cell Biol (1977) 1.30
Proteins of the postsynaptic density. J Cell Biol (1974) 1.27
Promotion of fluorescence upon binding of colchicine to tubulin. Proc Natl Acad Sci U S A (1974) 1.27
Human lymphocyte surface immunoglobulin capping. Normal characteristics and anomalous behavior of chronic lymphocytic leukemic lymphocytes. J Clin Invest (1975) 1.26
Properties of tubulin in unfertilized sea urchin eggs. Quantitation and characterization by the colchicine-binding reaction. J Cell Biol (1976) 1.25
The susceptibility of pure tubulin to high magnetic fields: a magnetic birefringence and x-ray fiber diffraction study. Biophys J (1998) 1.24
The binding in vitro of colchicine to axoplasmic proteins from chicken sciatic nerve. Biochem J (1970) 1.24
Cells injected with guanosine 5'-[alpha, beta-methylene]triphosphate, an alpha, beta-nonhydrolyzable analog of GTP, show anomalous patterns of tubulin polymerization affecting cell translocation, intracellular movement, and the organization of Golgi elements. Proc Natl Acad Sci U S A (1983) 1.23
Major nonhistone proteins of rat liver chromatin: preliminary identification of myosin, actin, tubulin, and tropomyosin. Proc Natl Acad Sci U S A (1975) 1.23
Effects of colchicine, cytochalasin B, and 2-deoxyglucose on the topographical organization of surface-bound concanavalin A in normal and transformed fibroblasts. J Cell Biol (1974) 1.19
In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB. J Cell Biol (2005) 1.18
The melanocyte model. Colchicine-like effects of other antimitotic agents. J Cell Biol (1971) 1.17
Stathmin and interfacial microtubule inhibitors recognize a naturally curved conformation of tubulin dimers. J Biol Chem (2010) 1.15
Role of nucleotides in tubulin polymerization: effect of guanylyl 5'-methylenediphosphonate. Proc Natl Acad Sci U S A (1977) 1.15
The assessment of drug-dependent and isoimmune antiplatelet antibodies by the use of platelet aggregometry. J Clin Invest (1972) 1.11
Purification of intact microtubules from brain. J Cell Biol (1970) 1.11
The association between phosphatidylinositol phosphodiesterase activity and a specific subunit of microtubular protein in rat brain. Biochem J (1973) 1.10
Eukaryotic cells and their cell bodies: Cell Theory revised. Ann Bot (2004) 1.10
Antisera against electrophoretically purified tubulin stimulate colchicine-binding activity. Proc Natl Acad Sci U S A (1976) 1.07
Design features of a mitotic spindle: balancing tension and compression at a single microtubule kinetochore interface in budding yeast. Annu Rev Genet (2008) 1.07
The PN2-3 domain of centrosomal P4.1-associated protein implements a novel mechanism for tubulin sequestration. J Biol Chem (2009) 1.06
Stoichiometry of GTP hydrolysis and tubulin polymerization. Proc Natl Acad Sci U S A (1977) 1.05
Mode of Dinitroaniline Herbicide Action: II. CHARACTERIZATION OF [C]ORYZALIN UPTAKE AND BINDING. Plant Physiol (1980) 1.05
Local anesthetic-induced inhibition of collagen secretion in cultured cells under conditions where microtubules are not depolymerized by these agents. J Cell Biol (1979) 1.04
Tubulins from different higher plant species are immunologically nonidentical and bind colchicine differentially. Proc Natl Acad Sci U S A (1984) 1.04
Tubulin constancy during morphological differentiation of mouse neuroblastoma cells. J Cell Biol (1975) 1.03
Role of GTP hydrolysis in microtubule treadmilling and assembly. Proc Natl Acad Sci U S A (1981) 1.03
Interaction of nerve growth factor with the mouse-brain neurotubule protein(s). Proc Natl Acad Sci U S A (1974) 1.00
The reciprocal exclusion by L-dopa (L-3,4-dihydroxyphenylalanine) and L-tyrosine of their incorporation as single units into a soluble rat brain protein. Biochem J (1975) 1.00
Quantitative assessment of polymerized and depolymerized platelet microtubules. Changes caused by aggregating agents. J Clin Invest (1979) 0.99
Differences in alpha and beta polypeptide chains of tubulin resolved by electron microscopy with image reconstruction. Proc Natl Acad Sci U S A (1978) 0.98
Dynamic instability 30 years later: complexities in microtubule growth and catastrophe. Mol Biol Cell (2015) 0.98
Nerve fibers in culture and their interactions with non-neural cells visualized by immunofluorescence. J Cell Biol (1979) 0.98
Cell elongation in the cultured embryonic chick lens epithelium with and without protein synthesis. Involvement of microtubules. J Cell Biol (1972) 0.97
Tubulin aggregation and disaggregation: mediation by two distinct vinblastine-binding sites. Proc Natl Acad Sci U S A (1976) 0.97
Interactions of a photoaffinity analog of GTP with the proteins of microtubules. Proc Natl Acad Sci U S A (1977) 0.96
Selective extraction of isolated mitotic apparatus. Evidence that typical microtubule protein is extracted by organic mercurial. J Cell Biol (1971) 0.95
Colchicine-binding protein of the liver. Its characterization and relation to microtubules. J Cell Biol (1975) 0.94
Molecules in mammalian brain that interact with the colchicine site on tubulin. Proc Natl Acad Sci U S A (1979) 0.94
Microtubule formation in vitro in solutions containing low calcium concentrations. Science (1972) 10.60
Mechanism of adenosine triphosphate hydrolysis by actomyosin. Biochemistry (1971) 9.73
The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein. J Cell Biol (1967) 9.66
Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. J Cell Biol (1999) 8.24
A structural change in the kinesin motor protein that drives motility. Nature (1999) 6.92
The mechanism of action of colchicine. Colchicine binding to sea urchin eggs and the mitotic apparatus. J Cell Biol (1967) 6.85
Microtubules. Annu Rev Biochem (1973) 6.75
Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation. J Cell Biol (1997) 6.67
Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro. Proc Natl Acad Sci U S A (1975) 6.35
Nucleated assembly of microtubules in porcine brain extracts. Science (1972) 6.22
Head-to-tail polymerization of microtubules in vitro. Electron microscope analysis of seeded assembly. J Cell Biol (1980) 5.64
Isolation of a protein subunit from microtubules. J Cell Biol (1967) 5.37
Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro. Ann N Y Acad Sci (1975) 5.26
Structural polarity and directional growth of microtubules of Chlamydomonas flagella. J Mol Biol (1974) 4.80
Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends. J Cell Biol (1987) 4.52
The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation. J Cell Biol (1977) 4.41
Functional coordination of microtubule-based and actin-based motility in melanophores. Curr Biol (1998) 4.03
Energetics and mechanism of actomyosin adenosine triphosphatase. Biochemistry (1976) 3.97
Self-polarization and directional motility of cytoplasm. Curr Biol (1999) 3.93
Kinetochore microtubule dynamics and the metaphase-anaphase transition. J Cell Biol (1995) 3.86
Mechanism of actomyosin ATPase and the problem of muscle contraction. CRC Crit Rev Biochem (1979) 3.83
Microtubule assembly in vitro. Fed Proc (1974) 3.82
Myosin II filament assemblies in the active lamella of fibroblasts: their morphogenesis and role in the formation of actin filament bundles. J Cell Biol (1995) 3.55
Direct observation of microtubule dynamics in living cells. Nature (1988) 3.47
Cytoplasmic filaments and tubules. Fed Proc (1968) 3.22
Centriole cycle in Chinese hamster ovary cells as determined by whole-mount electron microscopy. J Cell Biol (1981) 3.17
Kinetic analysis of microtubule self-assembly in vitro. J Mol Biol (1977) 3.15
Microtubule release from the centrosome. Proc Natl Acad Sci U S A (1997) 2.97
High throughput protein fold identification by using experimental constraints derived from intramolecular cross-links and mass spectrometry. Proc Natl Acad Sci U S A (2000) 2.96
Properties of the protein subunit of central-pair and outer-doublet microtubules of sea urchin flagella. J Cell Biol (1968) 2.91
Isolated flagellar apparatus of Chlamydomonas: characterization of forward swimming and alteration of waveform and reversal of motion by calcium ions in vitro. J Cell Sci (1978) 2.91
Phosphoproteins are components of mitotic microtubule organizing centers. Proc Natl Acad Sci U S A (1984) 2.84
Polarity of microtubules nucleated by centrosomes and chromosomes of Chinese hamster ovary cells in vitro. J Cell Biol (1980) 2.75
Kinetic studies of the cooperative binding of subfragment 1 to regulated actin. Proc Natl Acad Sci U S A (1980) 2.74
Polymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes. J Cell Biol (1985) 2.72
Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules. Biochemistry (1976) 2.69
Characterization of microtubule assembly in porcine brain extracts by viscometry. Biochemistry (1973) 2.68
On the mechanisms of cytokinesis in animal cells. J Theor Biol (1983) 2.63
Correlative light and electron microscopy of the cytoskeleton of cultured cells. Methods Enzymol (1998) 2.59
Interacting head mechanism of microtubule-kinesin ATPase. J Biol Chem (1997) 2.57
Microtubule dynamics in vivo: a test of mechanisms of turnover. J Cell Biol (1987) 2.56
In vitro polymerization of microtubules into asters and spindles in homogenates of surf clam eggs. J Cell Biol (1975) 2.53
Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis. J Cell Biol (1990) 2.46
Comparison of the myosin and actomyosin ATPase mechanisms of the four types of vertebrate muscles. J Mol Biol (1980) 2.46
Microtubule-nucleating activity of centrosomes in Chinese hamster ovary cells is independent of the centriole cycle but coupled to the mitotic cycle. J Cell Biol (1981) 2.43
Dendritic organization of actin comet tails. Curr Biol (2001) 2.43
Ionic and nucleotide requirements for microtubule polymerization in vitro. Biochemistry (1975) 2.43
Role of tubulin-associated proteins in microtubule nucleation and elongation. J Mol Biol (1977) 2.38
The non-tubulin component of microtubule protein oligomers. Effect on self-association and hydrodynamic properties. J Biol Chem (1978) 2.36
Intermediate states of subfragment 1 and actosubfragment 1 ATPase: reevaluation of the mechanism. Biochemistry (1978) 2.35
Plectin sidearms mediate interaction of intermediate filaments with microtubules and other components of the cytoskeleton. J Cell Biol (1996) 2.35
Microtubule dynamics and chromosome motion visualized in living anaphase cells. J Cell Biol (1988) 2.31
Microtuble assembly: some possible regulatory mechanisms. J Supramol Struct (1974) 2.21
Differential turnover of tyrosinated and detyrosinated microtubules. Proc Natl Acad Sci U S A (1987) 2.15
Transient state phosphate production in the hydrolysis of nucleoside triphosphates by myosin. Biochemistry (1970) 2.11
Non-sarcomeric mode of myosin II organization in the fibroblast lamellum. J Cell Biol (1993) 2.10
Mode of centriole duplication and distribution. J Cell Biol (1990) 2.07
Identity and polymerization-stimulatory activity of the nontubulin proteins associated with microtubules. Biochemistry (1977) 2.07
Proposed definitions for the audit of postoperative infection: a discussion paper. Surgical Infection Study Group. Ann R Coll Surg Engl (1991) 2.06
Microtubule treadmilling in vivo. Science (1997) 2.05
Polarity of microtubules of the mitotic spindle. J Mol Biol (1978) 2.02
Self-assembly of microtubules in extracts of cultured HeLa cells and the identification of HeLa microtubule-associated proteins. Proc Natl Acad Sci U S A (1979) 1.99
Further purification and characterization of slime mold myosin and slime mold actin. Biochemistry (1969) 1.96
Management of abdominal aortic aneurysm. Br J Surg (1978) 1.95
Improved procedures for electron microscopic visualization of the cytoskeleton of cultured cells. J Struct Biol (1996) 1.93
Survey of operating theatres in Great Britain and Ireland. J Hosp Infect (1995) 1.92
Early return to work after repair of a unilateral inguinal hernia. Br J Surg (1983) 1.89
Colcemid inhibition of cell growth and the characterization of a colcemid-binding activity in Saccharomyces cerevisiae. J Cell Biol (1972) 1.89
Microtubule dynamics at the G2/M transition: abrupt breakdown of cytoplasmic microtubules at nuclear envelope breakdown and implications for spindle morphogenesis. J Cell Biol (1996) 1.88
Origin of kinetochore microtubules in Chinese hamster ovary cells. Chromosoma (1980) 1.87
Immunofluorescence localization of HeLa cell microtubule-associated proteins on microtubules in vitro and in vivo. J Cell Biol (1980) 1.87
Isolation of an actomyosin-like protein complex from slime mold plasmodium and the separation of the complex into actin- and myosin-like fractions. Biochemistry (1969) 1.86
Microtubules are acetylated in domains that turn over slowly. J Cell Sci (1989) 1.86
Growth and lability of Chaetopterus oocyte mitotic spindles isolated in the presence of porcine brain tubulin. J Cell Biol (1974) 1.85
Centriole distribution during tripolar mitosis in Chinese hamster ovary cells. J Cell Biol (1984) 1.83
Kinetic mechanism of a monomeric kinesin construct. J Biol Chem (1997) 1.81
Immunostructural evidence for the template mechanism of microtubule nucleation. Nat Cell Biol (2000) 1.81
Transient kinetics of adenosine 5'-diphosphate and adenosine 5'-(beta, gamma-imidotriphosphate) binding to subfragment 1 and actosubfragment 1. Biochemistry (1982) 1.77
Widespread distribution of a 210,000 mol wt microtubule-associated protein in cells and tissues of primates. J Cell Biol (1980) 1.74
The ATPase mechanism of skeletal and smooth muscle acto-subfragment 1. J Biol Chem (1984) 1.71
A rapid method for quantitative determination of microtubule protein using DEAE-cellulose filters. Anal Biochem (1972) 1.70
Removal of the projections from cytoplasmic microtubules in vitro by digestion with trypsin. J Biol Chem (1977) 1.65
Microtubules of the kinetochore fiber turn over in metaphase but not in anaphase. J Cell Biol (1989) 1.64
Centrosomal and non-centrosomal microtubules. Biol Cell (1999) 1.64
Structure of kinetochore fibers: microtubule continuity and inter-microtubule bridges. Chromosoma (1981) 1.64
The attachment of kinetochores to the pro-metaphase spindle in PtK1 cells. Recovery from low temperature treatment. Chromosoma (1981) 1.64
Two components of actin-based retrograde flow in sea urchin coelomocytes. Mol Biol Cell (1999) 1.63
Changes in the organization of tubulin during meiosis in the eggs of the surf clam, Spisula solidissima. J Cell Biol (1972) 1.62
Studies on the kinetics of formation and dissociation of the actomyosin complex. Biochemistry (1969) 1.60
Transient phase of adenosine triphosphate hydrolysis by myosin, heavy meromyosin, and subfragment 1. Biochemistry (1977) 1.60
Speckle microscopic evaluation of microtubule transport in growing nerve processes. Nat Cell Biol (1999) 1.60
Self-organization of a propulsive actin network as an evolutionary process. Proc Natl Acad Sci U S A (2001) 1.60
Chemistry of muscle contraction. Annu Rev Biochem (1972) 1.59
Steady state dynamics of intermediate filament networks. J Cell Biol (1992) 1.59
Proteins of the mammalian mitotic spindle: phosphorylation/dephosphorylation of MAP-4 during mitosis. J Cell Sci (1991) 1.58