Published in Biochemistry on July 31, 1973
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol (1978) 75.38
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A (1977) 41.76
Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A (1974) 32.46
Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A (1979) 27.20
Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol (1974) 23.54
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res (1974) 19.42
Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol (1975) 13.95
pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res (1978) 13.22
Physical properties and gel electrophoresis behavior of R12-derived plasmid DNAs. Nucleic Acids Res (1977) 13.19
Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A (1974) 10.51
Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bacteriol (1978) 10.31
Folding of the DNA double helix in chromatin-like structures from simian virus 40. Proc Natl Acad Sci U S A (1975) 9.70
Construction and analysis of viable deletion mutants of simian virus 40. J Virol (1976) 9.11
Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res (1979) 8.00
Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A (1980) 7.80
Helper-independent transformation by unintegrated Harvey sarcoma virus DNA. J Virol (1978) 7.75
Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40. Proc Natl Acad Sci U S A (1975) 7.57
Packaging recombinant DNA molecules into bacteriophage particles in vitro. Proc Natl Acad Sci U S A (1977) 7.42
Polyoma DNA: a physical map. Proc Natl Acad Sci U S A (1974) 7.36
Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis. Proc Natl Acad Sci U S A (1977) 7.09
The inverted repeat as a recognizable structural feature in supercoiled DNA molecules. Proc Natl Acad Sci U S A (1980) 6.83
Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli. Proc Natl Acad Sci U S A (1979) 6.81
Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles. Proc Natl Acad Sci U S A (1975) 6.68
Specificity of Hpa II and Hae III DNA methylases. Nucleic Acids Res (1977) 6.62
A restriction map of the bacteriophage T4 genome. Mol Gen Genet (1980) 6.37
A method for the recovery of DNA from agarose gels. Nucleic Acids Res (1978) 6.24
Purified Escherichia coli recA protein catalyzes homologous pairing of superhelical DNA and single-stranded fragments. Proc Natl Acad Sci U S A (1979) 5.91
Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions. Proc Natl Acad Sci U S A (1976) 5.81
Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes. Nucleic Acids Res (1978) 5.62
Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A (1974) 5.47
Apoptosis. The role of the endonuclease. Am J Pathol (1990) 5.43
In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites. Proc Natl Acad Sci U S A (1978) 5.32
Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences. Proc Natl Acad Sci U S A (1977) 5.28
T antigen binds to simian virus 40 DNA at the origin of replication. Proc Natl Acad Sci U S A (1975) 5.22
Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases. Proc Natl Acad Sci U S A (1976) 4.92
Simple Mendelian inheritance of the reiterated ribosomal DNA of yeast. Proc Natl Acad Sci U S A (1977) 4.86
Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proc Natl Acad Sci U S A (1975) 4.79
Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus. Proc Natl Acad Sci U S A (1976) 4.74
Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences. Proc Natl Acad Sci U S A (1977) 4.64
Dual evolutionary origin for the rat genetic sequences of Harvey murine sarcoma virus. J Virol (1980) 4.63
Structure of the joint region and the termini of the DNA of herpes simplex virus type 1. J Virol (1978) 4.62
Biochemical procedure for production of small deletions in simian virus 40 DNA. Proc Natl Acad Sci U S A (1975) 4.59
Characterization of purified DNA-relaxing enzyme from human tissue culture cells. Proc Natl Acad Sci U S A (1975) 4.39
Localization of gene functions in polyoma virus DNA. Proc Natl Acad Sci U S A (1976) 4.38
High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene. Proc Natl Acad Sci U S A (1979) 4.37
Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA. Proc Natl Acad Sci U S A (1977) 4.35
The isolation of simian virus 40 variants with specifically altered genomes. Proc Natl Acad Sci U S A (1974) 4.35
Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper. Nucleic Acids Res (1980) 4.30
A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum. Nucleic Acids Res (1978) 4.25
Restriction enzymes and their isoschizomers. Nucleic Acids Res (1990) 4.24
Physical and genetic characterization of deletion mutants of simian virus 40 constructed in vitro. J Virol (1977) 4.23
Anatomy of herpes simplex virus DNA. III. Characterization of defective DNA molecules and biological properties of virus populations containing them. J Virol (1975) 4.19
The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases. Nucleic Acids Res (1974) 4.17
Somatic cells efficiently join unrelated DNA segments end-to-end. Mol Cell Biol (1982) 4.13
Regulation of early and late simian virus 40 transcription: overproduction of early viral RNA in the absence of a functional T-antigen. J Virol (1977) 3.96
tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis. Nucleic Acids Res (1982) 3.93
Molecular cloning of the Harvey sarcoma virus closed circular DNA intermediates: initial structural and biological characterization. J Virol (1979) 3.90
Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. J Bacteriol (1975) 3.88
Characterization of two types of yeast ribosomal DNA genes. J Bacteriol (1978) 3.87
PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR. Nucleic Acids Res (1996) 3.74
Viable deletion mutants of simian virus 40: selective isolation by means of a restriction endonuclease from Hemophilus parainfluenzae. Proc Natl Acad Sci U S A (1974) 3.71
Generalized Transduction in CAULOBACTER CRESCENTUS. Genetics (1977) 3.62
Physical maps for Herpes simplex virus type 1 DNA for restriction endonucleases Hind III, Hpa-1, and X. bad. J Virol (1976) 3.56
Isolation and characterization of polyoma host range mutants that replicate in nullipotential embryonal carcinoma cells. Proc Natl Acad Sci U S A (1981) 3.55
Effect of Fv-1 gene product on synthesis of linear and supercoiled viral DNA in cells infected with murine leukemia virus. J Virol (1980) 3.54
Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res (1985) 3.53
Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses. Proc Natl Acad Sci U S A (1982) 3.48
Cloning of a DNA fragment from the left-hand terminus of the adenovirus type 2 genome and its use in site-directed mutagenesis. J Virol (1981) 3.36
Structure and function of herpesvirus genomes. I. comparison of five HSV-1 and two HSV-2 strains by cleavage their DNA with eco R I restriction endonuclease. J Virol (1975) 3.32
Formation and structure of infectious DNA of spleen necrosis virus. J Virol (1977) 3.29
A multifunctional gene (tetR) controls Tn10-encoded tetracycline resistance. J Bacteriol (1982) 3.19
A cleavage map of bacteriophage phiX174 genome. Proc Natl Acad Sci U S A (1974) 3.13
The DNA sequence at the T7 C promoter. Nucleic Acids Res (1979) 3.10
Infectious viral DNA of murine leukemia virus. Proc Natl Acad Sci U S A (1975) 3.09
DNA of minute virus of mice: self-priming, nonpermuted, single-stranded genome with a 5'-terminal hairpin duplex. J Virol (1976) 3.08
Size fractionation of double-stranded DNA by precipitation with polyethylene glycol. Nucleic Acids Res (1975) 3.06
Physical map of polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegyptius, endonuclease R-HaeIII. J Virol (1975) 3.05
Transcription of simian virus 40. 3. Mapping of "early" and "late" species of RNA. Proc Natl Acad Sci U S A (1973) 3.04
Molecular cloning of the avian erythroblastosis virus genome and recovery of oncogenic virus by transfection of chicken cells. J Virol (1980) 3.01
The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene. Nucleic Acids Res (1983) 2.94
EcoRI restriction endonuclease map of the composite R plasmid NR1. J Bacteriol (1976) 2.90
Plasmid vehicles for direct cloning of Escherichia coli promoters. J Bacteriol (1979) 2.87
Purification of specific adenovirus 2 RNAs by preparative hybridization and selective thermal elution. Nucleic Acids Res (1979) 2.78
Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. J Virol (1974) 2.77
Molecular cloning and expression of Bacillus licheniformis beta-lactamase gene in Escherichia coli and Bacillus subtilis. J Bacteriol (1981) 2.75
Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J Virol (1981) 2.74
In vivo site-specific genetic recombination promoted by the EcoRI restriction endonuclease. Proc Natl Acad Sci U S A (1977) 2.74
Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes. Proc Natl Acad Sci U S A (1980) 2.74
Cloning and nucleotide sequence of DNA coding for bovine preproparathyroid hormone. Proc Natl Acad Sci U S A (1979) 2.72
Structure, subunit composition, and molecular weight of RD-114 RNA. J Virol (1975) 2.66
Variable-sized free episomes of Shope papilloma virus DNA are present in all non-virus-producing neoplasms and integrated episomes are detected in some. Proc Natl Acad Sci U S A (1982) 2.65
The nucleotide sequence at the termini of adenovirus type 5 DNA. Nucleic Acids Res (1977) 2.61
The cleavage site of the restriction endonuclease Ava II. Nucleic Acids Res (1978) 2.60
Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res (1983) 2.59
Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res (1980) 2.56
Contacts between the lac repressor and the thymines in the lac operator. Proc Natl Acad Sci U S A (1977) 2.52
Arrangement of the genes coding for ribosomal ribonucleic acids in Neurospora crassa. J Bacteriol (1979) 2.52
The presence of ovalbumin mRNA coding sequences in multiple restriction fragments of chicken DNA. Nucleic Acids Res (1977) 2.52
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell (1977) 61.24
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc Natl Acad Sci U S A (1980) 24.21
RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell (2000) 20.32
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol (1972) 19.88
Protein folding in the cell. Nature (1992) 19.60
Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A (1978) 18.67
Splicing of messenger RNA precursors. Annu Rev Biochem (1986) 14.41
Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells. Nature (1985) 14.39
A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature (1986) 14.34
Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc Natl Acad Sci U S A (1977) 14.34
Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs. Cell (1979) 12.83
The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell (1976) 12.60
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol (1973) 11.40
A cis-acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids in latently infected cells. Proc Natl Acad Sci U S A (1984) 10.91
Structure of the adenovirus 2 early mRNAs. Cell (1978) 10.54
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell (1985) 10.06
Five intermediate complexes in transcription initiation by RNA polymerase II. Cell (1989) 9.22
Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport. Cell (1986) 9.16
Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerases. I. Purification and properties of an -amanitin-sensitive ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells. J Biol Chem (1973) 8.86
Recognition of cap structure in splicing in vitro of mRNA precursors. Cell (1984) 8.67
A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes. Nature (1986) 8.64
The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A (1968) 8.38
Amount of viral DNA in the genome of cells transformed by adenovirus type 2. J Mol Biol (1973) 8.28
Speculations on RNA splicing. Cell (1981) 8.28
Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev (1999) 8.21
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell (1986) 8.06
Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA. Cell (1988) 8.00
The presence of malfolded proteins in the endoplasmic reticulum signals the induction of glucose-regulated proteins. Nature (1988) 7.98
Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A (1973) 7.75
A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus. Mol Cell Biol (1985) 7.66
Splicing of messenger RNA precursors. Science (1987) 7.61
Genetic analysis of immortalizing functions of Epstein-Barr virus in human B lymphocytes. Nature (1989) 7.60
Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J Mol Biol (1972) 7.49
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor. Mol Cell Biol (1986) 7.49
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes. Cell (1987) 7.44
Regulation by HIV Rev depends upon recognition of splice sites. Cell (1989) 7.13
In vitro transcription of adenovirus. J Virol (1981) 7.06
A transmembrane protein with a cdc2+/CDC28-related kinase activity is required for signaling from the ER to the nucleus. Cell (1993) 6.82
A putative origin of replication of plasmids derived from Epstein-Barr virus is composed of two cis-acting components. Mol Cell Biol (1985) 6.44
A multicomponent complex is involved in the splicing of messenger RNA precursors. Cell (1985) 6.27
Evolutionary fates and origins of U12-type introns. Mol Cell (1998) 6.25
The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products. Genes Dev (1988) 6.23
trans activation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1. Mol Cell Biol (1986) 6.00
Site-specific modification of pre-mRNA: the 2'-hydroxyl groups at the splice sites. Science (1992) 5.87
Human CCAAT-binding proteins have heterologous subunits. Cell (1988) 5.81
A high-efficiency HeLa cell nuclear transcription extract. DNA (1988) 5.81
Identification and characterization of oriLyt, a lytic origin of DNA replication of Epstein-Barr virus. Cell (1988) 5.76
Heterogeneous nuclear ribonucleoproteins: role in RNA splicing. Science (1986) 5.52
Accurate cleavage and polyadenylation of exogenous RNA substrate. Cell (1985) 5.27
Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors. Science (1984) 5.11
Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene. Mol Cell Biol (1987) 5.05
Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harb Symp Quant Biol (1975) 4.87
Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter. Proc Natl Acad Sci U S A (1988) 4.83
T antigen repression of SV40 early transcription from two promoters. Cell (1981) 4.83
Separation and characterization of factors mediating accurate transcription by RNA polymerase II. J Biol Chem (1982) 4.71
On the origin of RNA splicing and introns. Cell (1985) 4.70
Sequence heterogeneity in closed simian virus 40 deoxyribonucleic acid. J Virol (1972) 4.69
Splicing of adenovirus RNA in a cell-free transcription system. Proc Natl Acad Sci U S A (1983) 4.69
Origin-defective mutants of SV40. Cold Spring Harb Symp Quant Biol (1980) 4.64
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev (1989) 4.53
Studies on simian virus 40 excision from cellular chromosomes. Cold Spring Harb Symp Quant Biol (1979) 4.45
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. Cell (1983) 4.42
Expression of early adenovirus genes requires a viral encoded acidic polypeptide. Proc Natl Acad Sci U S A (1981) 4.42
The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element. Nature (1982) 4.42
Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol (1974) 4.30
S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP. Cell (1989) 4.28
HIV-1 Tat protein promotes formation of more-processive elongation complexes. EMBO J (1991) 4.18
The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains. Genes Dev (1988) 4.14
In vitro transcription: whole-cell extract. Methods Enzymol (1983) 4.07
Affinity chromatography of splicing complexes: U2, U5, and U4 + U6 small nuclear ribonucleoprotein particles in the spliceosome. Science (1986) 4.01
Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate. Cell (1984) 4.00
An improved bacteriophage lambda vector: construction of model recombinants coding for kanamycin resistance. Gene (1977) 3.94
Function of a yeast TATA element-binding protein in a mammalian transcription system. Nature (1988) 3.91
Agarose slab-gel electrophoresis equipment. Anal Biochem (1975) 3.88
Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol (1975) 3.87
Transcription maps of adenovirus. Methods Enzymol (1980) 3.82
Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA. Cell (1979) 3.78
DNA topology and a minimal set of basal factors for transcription by RNA polymerase II. Cell (1993) 3.77
RNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor. Proc Natl Acad Sci U S A (1979) 3.75
Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein. Nature (1982) 3.74
Site-specific polyadenylation in a cell-free reaction. Cell (1984) 3.59
HIV-1 Tat protein trans-activates transcription in vitro. Cell (1990) 3.57
Characterization of cDNAs encoding the polypyrimidine tract-binding protein. Genes Dev (1991) 3.55
SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen. Gene (1981) 3.54
Interactions between RNA polymerase II, factors, and template leading to accurate transcription. J Biol Chem (1984) 3.47
Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc Natl Acad Sci U S A (1991) 3.43
Transformation of Balb 3T3 cells by the BNLF-1 gene of Epstein-Barr virus. Oncogene (1988) 3.42
Physical mapping of temperature-sensitive mutations of adenoviruses. Cold Spring Harb Symp Quant Biol (1975) 3.42
The octamer-binding proteins form multi-protein--DNA complexes with the HSV alpha TIF regulatory protein. EMBO J (1989) 3.42
Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein. Proc Natl Acad Sci U S A (1988) 3.38
Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing. Nature (1993) 3.36
Generation of adenovirus by transfection of plasmids. Nucleic Acids Res (1983) 3.33
Cell-surface expression of influenza haemagglutinin from a cloned DNA copy of the RNA gene. Nature (1981) 3.29
The promoter region of the yeast KAR2 (BiP) gene contains a regulatory domain that responds to the presence of unfolded proteins in the endoplasmic reticulum. Mol Cell Biol (1993) 3.25
A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. Proc Natl Acad Sci U S A (1996) 3.25
Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions. Cell (1989) 3.18
Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences. Proc Natl Acad Sci U S A (1989) 3.18