Published in Science on August 31, 1984
Intronic microRNA precursors that bypass Drosha processing. Nature (2007) 9.65
Intron splicing: a conserved internal signal in introns of animal pre-mRNAs. Proc Natl Acad Sci U S A (1984) 4.71
RNA binding specificity of hnRNP proteins: a subset bind to the 3' end of introns. EMBO J (1988) 4.26
A catalogue of splice junction and putative branch point sequences from plant introns. Nucleic Acids Res (1986) 4.21
Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles. Mol Cell Biol (1988) 3.56
Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes. Mol Cell Biol (1987) 3.00
A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis. EMBO J (1992) 2.96
In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells. EMBO J (1991) 2.90
Polycistronic transcripts in trypanosomes and their accumulation during heat shock: evidence for a precursor role in mRNA synthesis. Mol Cell Biol (1988) 2.63
Ser/Thr-specific protein phosphatases are required for both catalytic steps of pre-mRNA splicing. Nucleic Acids Res (1992) 2.53
Intron splicing: a conserved internal signal in introns of Drosophila pre-mRNAs. Nucleic Acids Res (1985) 2.35
Ribonucleoprotein complex formation during pre-mRNA splicing in vitro. Mol Cell Biol (1986) 2.33
Spontaneous splicing mutations at the dihydrofolate reductase locus in Chinese hamster ovary cells. Mol Cell Biol (1986) 2.19
A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes. EMBO J (1994) 2.15
Precise localization of m6A in Rous sarcoma virus RNA reveals clustering of methylation sites: implications for RNA processing. Mol Cell Biol (1985) 2.06
Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing. RNA (2010) 1.85
U1 small nuclear ribonucleoproteins are required early during spliceosome assembly. Mol Cell Biol (1987) 1.83
Nonconsensus branch-site sequences in the in vitro splicing of transcripts of mutant rabbit beta-globin genes. Proc Natl Acad Sci U S A (1985) 1.82
Nuclear pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe strictly requires an intron-contained, conserved sequence element. EMBO J (1987) 1.82
Accurate in vitro splicing of two pre-mRNA plant introns in a HeLa cell nuclear extract. EMBO J (1986) 1.80
Heat shock but not other stress inducers leads to the disruption of a sub-set of snRNPs and inhibition of in vitro splicing in HeLa cells. EMBO J (1988) 1.78
Direct analysis of the mini-exon donor RNA of Trypanosoma brucei: detection of a novel cap structure also present in messenger RNA. Nucleic Acids Res (1987) 1.72
Recombinant hnRNP protein A1 and its N-terminal domain show preferential affinity for oligodeoxynucleotides homologous to intron/exon acceptor sites. Nucleic Acids Res (1990) 1.72
A nuclease that cuts specifically in the ribosome binding site of some T4 mRNAs. Proc Natl Acad Sci U S A (1988) 1.71
Noncoding region between the env and src genes of Rous sarcoma virus influences splicing efficiency at the src gene 3' splice site. J Virol (1987) 1.68
RNA splicing products formed with isolated fractions from HeLa cells are associated with fast-sedimenting complexes. Proc Natl Acad Sci U S A (1986) 1.67
T cell receptor-beta mRNA splicing: regulation of unusual splicing intermediates. Mol Cell Biol (1993) 1.63
Products of in vitro cleavage and polyadenylation of simian virus 40 late pre-mRNAs. Mol Cell Biol (1987) 1.61
Accurate and efficient pre-mRNA splicing in Drosophila cell-free extracts. Proc Natl Acad Sci U S A (1988) 1.60
Human adenine phosphoribosyltransferase. Identification of allelic mutations at the nucleotide level as a cause of complete deficiency of the enzyme. J Clin Invest (1987) 1.59
Purification of a protein required for the splicing of pre-mRNA and its separation from the lariat debranching enzyme. EMBO J (1985) 1.56
Fractionation and characterization of a yeast mRNA splicing extract. Proc Natl Acad Sci U S A (1986) 1.56
Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae. Nucleic Acids Res (1985) 1.54
Specific accessory sequences in Saccharomyces cerevisiae introns control assembly of pre-mRNAs into spliceosomes. EMBO J (1987) 1.53
Myosin light-chain 1 and 3 gene has two structurally distinct and differentially regulated promoters evolving at different rates. Mol Cell Biol (1985) 1.51
Inhibition of splicing but not cleavage at the 5' splice site by truncating human beta-globin pre-mRNA. Proc Natl Acad Sci U S A (1986) 1.51
The small subunit of the splicing factor U2AF is conserved in fission yeast. Nucleic Acids Res (1996) 1.48
The 216-nucleotide intron of the E1A pre-mRNA contains a hairpin structure that permits utilization of unusually distant branch acceptors. Mol Cell Biol (1989) 1.48
In vitro apolipoprotein B mRNA editing: identification of a 27S editing complex. Proc Natl Acad Sci U S A (1991) 1.47
Viroids and virusoids are related to group I introns. Proc Natl Acad Sci U S A (1986) 1.46
Forks and combs and DNA: the synthesis of branched oligodeoxyribonucleotides. Nucleic Acids Res (1989) 1.44
Branch point selection in alternative splicing of tropomyosin pre-mRNAs. Nucleic Acids Res (1989) 1.43
Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins. J Cell Biol (1986) 1.41
In vitro splicing of simian virus 40 early pre mRNA. Nucleic Acids Res (1986) 1.40
RNA splicing and intron turnover are greatly diminished by a mutant yeast branch point. Proc Natl Acad Sci U S A (1986) 1.39
Herpes simplex virus IE63 (ICP27) protein interacts with spliceosome-associated protein 145 and inhibits splicing prior to the first catalytic step. J Virol (2001) 1.38
Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe. Mol Cell Biol (1989) 1.35
Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro. Proc Natl Acad Sci U S A (1985) 1.30
Nuclear organization of splicing small nuclear ribonucleoproteins in adenovirus-infected cells. J Virol (1993) 1.28
Sequential recognition of the pre-mRNA branch point by U2AF65 and a novel spliceosome-associated 28-kDa protein. RNA (1995) 1.26
Alternative branch points are selected during splicing of a yeast pre-mRNA in mammalian and yeast extracts. Proc Natl Acad Sci U S A (1986) 1.25
In vitro splicing of influenza viral NS1 mRNA and NS1-beta-globin chimeras: possible mechanisms for the control of viral mRNA splicing. Proc Natl Acad Sci U S A (1986) 1.25
mRNA precursor splicing in vivo: sequence requirements determined by deletion analysis of an intervening sequence. Proc Natl Acad Sci U S A (1985) 1.25
Assembly in an in vitro splicing reaction of a mouse insulin messenger RNA precursor into a 60-40S ribonucleoprotein complex. Nucleic Acids Res (1986) 1.23
Yeast pre-messenger RNA splicing efficiency depends on critical spacing requirements between the branch point and 3' splice site. EMBO J (1986) 1.22
Sequence-specific affinity selection of mammalian splicing complexes. Nucleic Acids Res (1990) 1.22
The stability and fate of a spliced intron from vertebrate cells. RNA (1999) 1.22
Analysis of the 2-kilobase latency-associated transcript expressed in PC12 cells productively infected with herpes simplex virus type 1: evidence for a stable, nonlinear structure. J Virol (1997) 1.21
Effects on mRNA splicing of mutations in the 3' region of the Saccharomyces cerevisiae actin intron. Mol Cell Biol (1987) 1.21
2' phosphomonoester, 3'-5' phosphodiester bond at a unique site in a circular viral RNA. EMBO J (1985) 1.20
More than one way to splice an RNA: branching without a bulge and splicing without branching in group II introns. RNA (1998) 1.20
Splicing of the adenovirus-2 E1A 13S mRNA requires a minimal intron length and specific intron signals. Nucleic Acids Res (1985) 1.19
In vitro splicing of adenovirus E1A transcripts: characterization of novel reactions and of multiple branch points abnormally far from the 3' splice site. Nucleic Acids Res (1988) 1.19
The genes for yeast ribosomal proteins S24 and L46 are adjacent and divergently transcribed. Nucleic Acids Res (1985) 1.19
Unusual branch point selection involved in splicing of the alternatively processed Calcitonin/CGRP-I pre-mRNA. Nucleic Acids Res (1988) 1.18
Mammalian U6 small nuclear RNA undergoes 3' end modifications within the spliceosome. Mol Cell Biol (1993) 1.17
Alternate trans splicing in Trypanosoma equiperdum: implications for splice site selection. Mol Cell Biol (1988) 1.17
Isolation and characterization of an RNA ligase from HeLa cells. Proc Natl Acad Sci U S A (1985) 1.17
Structure-function analysis of yeast RNA debranching enzyme (Dbr1), a manganese-dependent phosphodiesterase. Nucleic Acids Res (2005) 1.17
Molecular consequences of truncations of the first exon for in vitro splicing of yeast actin pre-mRNA. Nucleic Acids Res (1988) 1.16
Alternative splicing of SV40 early pre-mRNA in vitro. Nucleic Acids Res (1986) 1.13
PRP28, a 'DEAD-box' protein, is required for the first step of mRNA splicing in vitro. Nucleic Acids Res (1994) 1.13
The Pleurotus ostreatus laccase multi-gene family: isolation and heterologous expression of new family members. Curr Genet (2008) 1.11
In vitro splicing of mRNA precursors: 5' cleavage site can be predicted from the interaction between the 5' splice region and the 5' terminus of U1 snRNA. Nucleic Acids Res (1988) 1.11
Novel deletion mutants that enhance a distant upstream 5' splice in the E3 transcription unit of adenovirus 2. Nucleic Acids Res (1985) 1.11
Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing. RNA (2007) 1.10
Structure of the yeast U2/U6 snRNA complex. RNA (2012) 1.09
Crystal structure of a eukaryotic group II intron lariat. Nature (2014) 1.09
The two intervening sequences of human beta- and gamma-globin pre-mRNAs are excised in a preferred temporal order in vitro. EMBO J (1985) 1.09
Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway. Proc Natl Acad Sci U S A (1986) 1.09
Model for alternative RNA processing in human calcitonin gene expression. Nucleic Acids Res (1986) 1.06
Activation of a cryptic TACTAAC box in the Saccharomyces cerevisiae actin intron. Mol Cell Biol (1986) 1.05
Control of branch-site choice by a group II intron. EMBO J (2001) 1.05
Evidence from complementation assays in vitro that U5 snRNP is required for both steps of mRNA splicing. EMBO J (1989) 1.03
Insights into branch nucleophile positioning and activation from an orthogonal pre-mRNA splicing system in yeast. Mol Cell (2009) 1.03
Splice site consensus sequences are preferentially accessible to nucleases in isolated adenovirus RNA. Nucleic Acids Res (1986) 1.02
Virus deletion mutants that affect a 3' splice site in the E3 transcription unit of adenovirus 2. Mol Cell Biol (1985) 1.01
The stereochemical course of the first step of pre-mRNA splicing. Nucleic Acids Res (1993) 1.00
A block in mammalian splicing occurring after formation of large complexes containing U1, U2, U4, U5, and U6 small nuclear ribonucleoproteins. Mol Cell Biol (1989) 0.99
Model for tissue specific Calcitonin/CGRP-I RNA processing from in vitro experiments. Nucleic Acids Res (1988) 0.98
Kluyveromyces lactis maintains Saccharomyces cerevisiae intron-encoded splicing signals. Mol Cell Biol (1989) 0.98
Beta zero thalassemia caused by a base substitution that creates an alternative splice acceptor site in an intron. EMBO J (1986) 0.97
Secondary structure of splice sites in adenovirus mRNA precursors. Nucleic Acids Res (1984) 0.96
Alternative processing of H-2Dd pre-mRNAs results in membrane expression of differentially phosphorylated protein products. EMBO J (1986) 0.95
Splicing of a C. elegans myosin pre-mRNA in a human nuclear extract. Nucleic Acids Res (1990) 0.95
New RNA-mediated reactions by yeast mitochondrial group I introns. EMBO J (1987) 0.95
The dependence of splicing efficiency on the length of 3' exon. Nucleic Acids Res (1988) 0.95
Exploitation of a thermosensitive splicing event to study pre-mRNA splicing in vivo. Mol Cell Biol (1988) 0.94
An evaluation of detection methods for large lariat RNAs. RNA (2005) 0.94
Identification and characterization of a gene that is coamplified with dihydrofolate reductase in a methotrexate-resistant CHO cell line. Mol Cell Biol (1989) 0.92
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell (1977) 61.24
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry (1973) 43.61
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc Natl Acad Sci U S A (1980) 24.21
RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell (2000) 20.32
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol (1972) 19.88
Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A (1978) 18.67
Splicing of messenger RNA precursors. Annu Rev Biochem (1986) 14.41
A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature (1986) 14.34
Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc Natl Acad Sci U S A (1977) 14.34
Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs. Cell (1979) 12.83
Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena. Cell (1982) 12.12
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol (1973) 11.40
Rapid DNA isolations for enzymatic and hybridization analysis. Methods Enzymol (1980) 11.28
Structure of the adenovirus 2 early mRNAs. Cell (1978) 10.54
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell (1985) 10.06
Five intermediate complexes in transcription initiation by RNA polymerase II. Cell (1989) 9.22
Recognition of cap structure in splicing in vitro of mRNA precursors. Cell (1984) 8.67
A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes. Nature (1986) 8.64
Speculations on RNA splicing. Cell (1981) 8.28
Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev (1999) 8.21
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell (1986) 8.06
Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA. Cell (1988) 8.00
Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A (1973) 7.75
Splicing of messenger RNA precursors. Science (1987) 7.61
Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J Mol Biol (1972) 7.49
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor. Mol Cell Biol (1986) 7.49
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes. Cell (1987) 7.44
Regulation by HIV Rev depends upon recognition of splice sites. Cell (1989) 7.13
In vitro transcription of adenovirus. J Virol (1981) 7.06
Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells. J Biol Chem (1979) 6.31
A multicomponent complex is involved in the splicing of messenger RNA precursors. Cell (1985) 6.27
Evolutionary fates and origins of U12-type introns. Mol Cell (1998) 6.25
The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products. Genes Dev (1988) 6.23
Site-specific modification of pre-mRNA: the 2'-hydroxyl groups at the splice sites. Science (1992) 5.87
Human CCAAT-binding proteins have heterologous subunits. Cell (1988) 5.81
A high-efficiency HeLa cell nuclear transcription extract. DNA (1988) 5.81
Heterogeneous nuclear ribonucleoproteins: role in RNA splicing. Science (1986) 5.52
Accurate cleavage and polyadenylation of exogenous RNA substrate. Cell (1985) 5.27
Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene. Mol Cell Biol (1987) 5.05
Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harb Symp Quant Biol (1975) 4.87
Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter. Proc Natl Acad Sci U S A (1988) 4.83
T antigen repression of SV40 early transcription from two promoters. Cell (1981) 4.83
Separation and characterization of factors mediating accurate transcription by RNA polymerase II. J Biol Chem (1982) 4.71
On the origin of RNA splicing and introns. Cell (1985) 4.70
Sequence heterogeneity in closed simian virus 40 deoxyribonucleic acid. J Virol (1972) 4.69
Splicing of adenovirus RNA in a cell-free transcription system. Proc Natl Acad Sci U S A (1983) 4.69
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev (1989) 4.53
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. Cell (1983) 4.42
In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence. Cell (1981) 4.42
Expression of early adenovirus genes requires a viral encoded acidic polypeptide. Proc Natl Acad Sci U S A (1981) 4.42
The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element. Nature (1982) 4.42
Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA. Cell (1986) 4.40
Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol (1974) 4.30
HIV-1 Tat protein promotes formation of more-processive elongation complexes. EMBO J (1991) 4.18
The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains. Genes Dev (1988) 4.14
In vitro transcription: whole-cell extract. Methods Enzymol (1983) 4.07
Affinity chromatography of splicing complexes: U2, U5, and U4 + U6 small nuclear ribonucleoprotein particles in the spliceosome. Science (1986) 4.01
Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate. Cell (1984) 4.00
An improved bacteriophage lambda vector: construction of model recombinants coding for kanamycin resistance. Gene (1977) 3.94
Function of a yeast TATA element-binding protein in a mammalian transcription system. Nature (1988) 3.91
Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol (1975) 3.87
Transcription maps of adenovirus. Methods Enzymol (1980) 3.82
DNA topology and a minimal set of basal factors for transcription by RNA polymerase II. Cell (1993) 3.77
RNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor. Proc Natl Acad Sci U S A (1979) 3.75
Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns. Science (1996) 3.61
Site-specific polyadenylation in a cell-free reaction. Cell (1984) 3.59
HIV-1 Tat protein trans-activates transcription in vitro. Cell (1990) 3.57
Characterization of cDNAs encoding the polypyrimidine tract-binding protein. Genes Dev (1991) 3.55
SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen. Gene (1981) 3.54
Interactions between RNA polymerase II, factors, and template leading to accurate transcription. J Biol Chem (1984) 3.47
Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc Natl Acad Sci U S A (1991) 3.43
The octamer-binding proteins form multi-protein--DNA complexes with the HSV alpha TIF regulatory protein. EMBO J (1989) 3.42
Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein. Proc Natl Acad Sci U S A (1988) 3.38
Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing. Nature (1993) 3.36
Generation of adenovirus by transfection of plasmids. Nucleic Acids Res (1983) 3.33
A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. Proc Natl Acad Sci U S A (1996) 3.25
Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions. Cell (1989) 3.18
Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences. Proc Natl Acad Sci U S A (1989) 3.18
Regulation of heat shock protein 70 gene expression by c-myc. Nature (1984) 3.18
Nucleotide sequence and evolution of a mammalian alpha-tubulin messenger RNA. J Mol Biol (1981) 3.18
Characterization of the branch site in lariat RNAs produced by splicing of mRNA precursors. Nature (1985) 3.17