Published in Cell on August 01, 1979
Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP175. J Virol (1980) 9.30
Transcription of adenovirus type 2 genes in a cell-free system: apparent heterogeneity of initiation at some promoters. Mol Cell Biol (1981) 7.12
In vitro transcription of adenovirus. J Virol (1981) 7.06
Both Epstein-Barr virus (EBV)-encoded trans-acting factors, EB1 and EB2, are required to activate transcription from an EBV early promoter. EMBO J (1986) 6.82
Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli. J Virol (1984) 6.73
trans activation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1. Mol Cell Biol (1986) 6.00
Stimulation of expression of a herpes simplex virus DNA-binding protein by two viral functions. Mol Cell Biol (1985) 5.76
Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products. J Virol (1985) 5.69
E1A control of gene expression is mediated by sequences 5' to the transcriptional starts of the early viral genes. Mol Cell Biol (1983) 4.84
Epstein-Barr virus RNA VII: size and direction of transcription of virus-specified cytoplasmic RNAs in a transformed cell line. Proc Natl Acad Sci U S A (1981) 4.77
Common control of the heat shock gene and early adenovirus genes: evidence for a cellular E1A-like activity. Mol Cell Biol (1984) 4.76
Regulation of herpesvirus macromolecular synthesis: transcription-initiation sites and domains of alpha genes. Proc Natl Acad Sci U S A (1980) 4.75
Role of an adenovirus E2 promoter binding factor in E1A-mediated coordinate gene control. Proc Natl Acad Sci U S A (1987) 4.68
Expression of early adenovirus genes requires a viral encoded acidic polypeptide. Proc Natl Acad Sci U S A (1981) 4.42
cis and trans activation of globin gene transcription in transient assays. Proc Natl Acad Sci U S A (1983) 4.41
Regulation of cytomegalovirus gene expression: alpha and beta promoters are trans activated by viral functions in permissive human fibroblasts. J Virol (1985) 4.39
A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan. Nucleic Acids Res (1989) 4.29
Transcriptional activation and subsequent control of the human heat shock gene during adenovirus infection. Mol Cell Biol (1983) 4.15
Association of adenovirus early-region 1A proteins with cellular polypeptides. Mol Cell Biol (1986) 4.14
A cellular protein, activating transcription factor, activates transcription of multiple E1A-inducible adenovirus early promoters. Proc Natl Acad Sci U S A (1987) 3.91
Promiscuous trans activation of gene expression by an Epstein-Barr virus-encoded early nuclear protein. J Virol (1986) 3.82
Construction of viable deletion and insertion mutants of the Sabin strain of type 1 poliovirus: function of the 5' noncoding sequence in viral replication. J Virol (1987) 3.79
A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences. Proc Natl Acad Sci U S A (1981) 3.68
Positive and negative autoregulation of the adeno-associated virus type 2 genome. J Virol (1986) 3.67
Identification of separate domains in the adenovirus E1A gene for immortalization activity and the activation of virus early genes. Mol Cell Biol (1986) 3.52
HeLa cell beta-tubulin gene transcription is stimulated by adenovirus 5 in parallel with viral early genes by an E1a-dependent mechanism. Mol Cell Biol (1984) 3.44
Cloning of a DNA fragment from the left-hand terminus of the adenovirus type 2 genome and its use in site-directed mutagenesis. J Virol (1981) 3.36
Negative and positive regulation in trans of gene expression from adeno-associated virus vectors in mammalian cells by a viral rep gene product. Mol Cell Biol (1986) 3.34
Adenovirus EIIA early promoter: transcriptional control elements and induction by the viral pre-early EIA gene, which appears to be sequence independent. Proc Natl Acad Sci U S A (1985) 3.32
Nucleotide sequence of adenovirus 2 DNA fragment encoding for the carboxylic region of the fiber protein and the entire E4 region. Nucleic Acids Res (1981) 3.31
The E2 "gene" of bovine papillomavirus encodes an enhancer-binding protein. Proc Natl Acad Sci U S A (1987) 3.31
Activation of early adenovirus transcription by the herpesvirus immediate early gene: evidence for a common cellular control factor. Proc Natl Acad Sci U S A (1982) 3.29
Functional mapping of a trans-activating gene required for expression of a baculovirus delayed-early gene. J Virol (1986) 3.28
Epstein-Barr virus RNA. VIII. Viral RNA in permissively infected B95-8 cells. J Virol (1982) 3.26
Stages in the nuclear association of the herpes simplex virus transcriptional activator protein ICP4. J Virol (1987) 3.24
Adenovirus early region 1A protein activates transcription of a nonviral gene introduced into mammalian cells by infection or transfection. Proc Natl Acad Sci U S A (1984) 3.24
An enhancer-like element in the adenovirus E2 promoter contains sequences essential for uninduced and E1A-induced transcription. Proc Natl Acad Sci U S A (1985) 3.15
Mapping of cellular protein-binding sites on the products of early-region 1A of human adenovirus type 5. Mol Cell Biol (1988) 3.07
Adenovirus 2 early region 1A stimulates expression of both viral and cellular genes. EMBO J (1984) 3.03
Determinants in the 5' noncoding region of poliovirus Sabin 1 RNA that influence the attenuation phenotype. J Virol (1989) 2.98
Identification of specific adenovirus E1A N-terminal residues critical to the binding of cellular proteins and to the control of cell growth. J Virol (1993) 2.92
Adenovirus 5 E2 transcription unit: an E1A-inducible promoter with an essential element that functions independently of position or orientation. Mol Cell Biol (1984) 2.88
The adenovirus-inducible factor E2F stimulates transcription after specific DNA binding. Mol Cell Biol (1989) 2.83
Nucleotide sequence and temporal expression of a baculovirus regulatory gene. J Virol (1987) 2.83
A cell line that supports the growth of a defective early region 4 deletion mutant of human adenovirus type 2. Proc Natl Acad Sci U S A (1983) 2.83
Identification of a factor in HeLa cells specific for an upstream transcriptional control sequence of an EIA-inducible adenovirus promoter and its relative abundance in infected and uninfected cells. Proc Natl Acad Sci U S A (1986) 2.81
Transcription of the human hsp70 gene is induced by serum stimulation. Proc Natl Acad Sci U S A (1985) 2.76
Adenovirus E1A coding sequences that enable ras and pmt oncogenes to transform cultured primary cells. Mol Cell Biol (1986) 2.70
Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region. EMBO J (1985) 2.69
E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA. Mol Cell Biol (1985) 2.68
Characterization of cellular factors that interact with the human T-cell leukemia virus type I p40x-responsive 21-base-pair sequence. J Virol (1988) 2.67
Identification and mapping of Epstein-Barr virus early antigens and demonstration of a viral gene activator that functions in trans. J Virol (1986) 2.64
Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level. Proc Natl Acad Sci U S A (1984) 2.64
The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1. J Virol (1987) 2.63
Adenovirus stimulation of transcription by RNA polymerase III: evidence for an E1A-dependent increase in transcription factor IIIC concentration. EMBO J (1986) 2.62
E1a regions of the human adenoviruses and of the highly oncogenic simian adenovirus 7 are closely related. J Virol (1985) 2.59
Transformation-defective mutant of adenovirus type 5 containing a single altered E1a mRNA species. J Virol (1981) 2.57
Analysis of an activatable promoter: sequences in the simian virus 40 late promoter required for T-antigen-mediated trans activation. Mol Cell Biol (1985) 2.55
Generation of novel, biologically active Harvey sarcoma viruses via apparent illegitimate recombination. J Virol (1981) 2.53
Molecular genetics of herpes simplex virus. VI. Characterization of a temperature-sensitive mutant defective in the expression of all early viral gene products. J Virol (1981) 2.52
Simple repeat sequence in Epstein-Barr virus DNA is transcribed in latent and productive infections. J Virol (1982) 2.51
gamma 2-Thymidine kinase chimeras are identically transcribed but regulated a gamma 2 genes in herpes simplex virus genomes and as beta genes in cell genomes. Mol Cell Biol (1985) 2.44
Regulation of early adenovirus transcription: a protein product of early region 2 specifically represses region 4 transcription. Proc Natl Acad Sci U S A (1980) 2.42
Regulation of transcription of the adenovirus EII promoter by EIa gene products: absence of sequence specificity. Mol Cell Biol (1984) 2.42
Transformation properties of type 5 adenovirus mutants that differentially express the E1A gene products. Proc Natl Acad Sci U S A (1984) 2.39
Different functional domains of the adenovirus E1A gene are involved in regulation of host cell cycle products. Mol Cell Biol (1987) 2.38
A human parvovirus, adeno-associated virus, as a eucaryotic vector: transient expression and encapsidation of the procaryotic gene for chloramphenicol acetyltransferase. Mol Cell Biol (1984) 2.36
Localization of the adenovirus E1Aa protein, a positive-acting transcriptional factor, in infected cells infected cells. Mol Cell Biol (1983) 2.36
Identification of human adenovirus early region 1 products by using antisera against synthetic peptides corresponding to the predicted carboxy termini. J Virol (1983) 2.35
Dissection of overlapping functions within the adenovirus type 5 E1A gene. EMBO J (1984) 2.30
Enhanced expression of adenovirus transforming proteins. J Virol (1982) 2.24
Activation of a preexisting cellular factor as a basis for adenovirus E1A-mediated transcription control. Proc Natl Acad Sci U S A (1988) 2.16
Lytic and transforming functions of individual products of the adenovirus E1A gene. J Virol (1986) 2.14
RNA encoded by the IR1-U2 region of Epstein-Barr virus DNA in latently infected, growth-transformed cells. J Virol (1983) 2.12
In vitro construction of poliovirus defective interfering particles. J Virol (1989) 2.10
Adenovirus type 5 virions can be assembled in vivo in the absence of detectable polypeptide IX. J Virol (1981) 2.07
Transcriptional analysis of the adenovirus-5 EIII promoter: absence of sequence specificity for stimulation by EIa gene products. Nucleic Acids Res (1985) 2.04
The E4 transcriptional unit of Ad2: far upstream sequences are required for its transactivation by E1A. Nucleic Acids Res (1984) 2.02
Cell cycle control of the human HSP70 gene: implications for the role of a cellular E1A-like function. Mol Cell Biol (1985) 2.01
Mutations in the gene encoding the adenovirus early region 1B 19,000-molecular-weight tumor antigen cause the degradation of chromosomal DNA. J Virol (1984) 2.00
Mutations in the major DNA-binding protein gene of herpes simplex virus type 1 result in increased levels of viral gene expression. J Virol (1983) 1.98
An adenovirus early region 1A protein is required for maximal viral DNA replication in growth-arrested human cells. J Virol (1985) 1.97
Adenovirus-induced alterations of the cell growth cycle: a requirement for expression of E1A but not of E1B. J Virol (1983) 1.93
Trans activation of plasmid-borne promoters by adenovirus and several herpes group viruses. Nucleic Acids Res (1984) 1.93
Functions of purified E1A protein microinjected into mammalian cells. Proc Natl Acad Sci U S A (1984) 1.92
Trans effect of the E1 region of adenoviruses on the expression of a prokaryotic gene in mammalian cells: resistance to 5' -CCGG- 3' methylation. Proc Natl Acad Sci U S A (1986) 1.92
mRNAs from human adenovirus 2 early region 4. J Virol (1984) 1.92
Expression of processed core protein of hepatitis C virus in mammalian cells. J Virol (1991) 1.91
Herpes simplex virus-induced changes in cellular and adenovirus RNA metabolism in an adenovirus type 5-transformed human cell line. J Virol (1982) 1.90
Sequence-independent autoregulation of the adenovirus type 5 E1A transcription unit. Mol Cell Biol (1985) 1.90
Regulation of herpesvirus macromolecular synthesis: temporal order of transcription of alpha genes is not dependent on the stringency of inhibition of protein synthesis. J Virol (1981) 1.88
Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome. J Virol (1985) 1.88
Expression of a proto-oncogene (proto-myb) in hemopoietic tissues of mice. Mol Cell Biol (1984) 1.85
The 289-amino acid E1A protein of adenovirus binds zinc in a region that is important for trans-activation. Proc Natl Acad Sci U S A (1988) 1.84
Two colinear and spliced viral transcripts are present in non-virus-producing benign and malignant neoplasms induced by the shope (rabbit) papilloma virus. J Virol (1982) 1.83
Downstream sequences affect transcription initiation from the adenovirus major late promoter. Mol Cell Biol (1986) 1.82
The E1A 13S product of adenovirus 5 activates transcription of the cellular human HSP70 gene. Mol Cell Biol (1986) 1.81
Adenovirus VA RNAI mediates a translational stimulation which is not restricted to the viral mRNAs. EMBO J (1985) 1.80
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell (1977) 61.24
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry (1973) 43.61
Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma. Science (1994) 39.60
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc Natl Acad Sci U S A (1980) 24.21
RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell (2000) 20.32
A protein interaction map of Drosophila melanogaster. Science (2003) 20.06
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol (1972) 19.88
Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A (1978) 18.67
An outbreak of multidrug-resistant tuberculosis among hospitalized patients with the acquired immunodeficiency syndrome. N Engl J Med (1992) 18.11
Splicing of messenger RNA precursors. Annu Rev Biochem (1986) 14.41
A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature (1986) 14.34
Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc Natl Acad Sci U S A (1977) 14.34
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol (1973) 11.40
Targeted disruption of the melanocortin-4 receptor results in obesity in mice. Cell (1997) 11.35
Structure of the adenovirus 2 early mRNAs. Cell (1978) 10.54
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell (1985) 10.06
Meta-analysis of 74,046 individuals identifies 11 new susceptibility loci for Alzheimer's disease. Nat Genet (2013) 9.46
Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells. Cell (1982) 9.27
Five intermediate complexes in transcription initiation by RNA polymerase II. Cell (1989) 9.22
The genome of the social amoeba Dictyostelium discoideum. Nature (2005) 9.18
Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells. J Mol Appl Genet (1983) 8.94
Recognition of cap structure in splicing in vitro of mRNA precursors. Cell (1984) 8.67
Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids. Nature (1981) 8.66
A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes. Nature (1986) 8.64
Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue. Virology (1978) 8.28
Speculations on RNA splicing. Cell (1981) 8.28
Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev (1999) 8.21
Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera. J Clin Microbiol (1988) 8.09
GPs in principle but not in practice: a study of vocationally trained doctors not currently working as principals. BMJ (1995) 8.09
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell (1986) 8.06
Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA. Cell (1988) 8.00
Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A (1973) 7.75
Splicing of messenger RNA precursors. Science (1987) 7.61
Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J Mol Biol (1972) 7.49
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor. Mol Cell Biol (1986) 7.49
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes. Cell (1987) 7.44
Regulation by HIV Rev depends upon recognition of splice sites. Cell (1989) 7.13
In vitro transcription of adenovirus. J Virol (1981) 7.06
Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli. J Virol (1984) 6.73
A quantitative approach to perceived health status: a validation study. J Epidemiol Community Health (1980) 6.29
A multicomponent complex is involved in the splicing of messenger RNA precursors. Cell (1985) 6.27
Evolutionary fates and origins of U12-type introns. Mol Cell (1998) 6.25
The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products. Genes Dev (1988) 6.23
Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities. Proc Natl Acad Sci U S A (1986) 6.16
Disruption of p53 in human cancer cells alters the responses to therapeutic agents. J Clin Invest (1999) 6.13
Direct inhibition of the signaling functions of the mammalian target of rapamycin by the phosphoinositide 3-kinase inhibitors, wortmannin and LY294002. EMBO J (1996) 6.08
Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination. Proc Natl Acad Sci U S A (1977) 6.08
Site-specific modification of pre-mRNA: the 2'-hydroxyl groups at the splice sites. Science (1992) 5.87
Adenovirus promoters and E1A transactivation. Annu Rev Genet (1986) 5.86
Human CCAAT-binding proteins have heterologous subunits. Cell (1988) 5.81
A high-efficiency HeLa cell nuclear transcription extract. DNA (1988) 5.81
Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells. Proc Natl Acad Sci U S A (1984) 5.81
Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site. Nature (1982) 5.61
Heterogeneous nuclear ribonucleoproteins: role in RNA splicing. Science (1986) 5.52
New features of the regulation of the tryptophan operon. Science (1975) 5.40
Cytokines: coordinators of immune and inflammatory responses. Annu Rev Biochem (1990) 5.36
Accurate cleavage and polyadenylation of exogenous RNA substrate. Cell (1985) 5.27
Prenatal screening for Down's syndrome with use of maternal serum markers. N Engl J Med (1992) 5.21
Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA. Nature (1983) 5.18
Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors. Science (1984) 5.11
High-efficiency cloning of full-length cDNA; construction and screening of cDNA expression libraries for mammalian cells. Methods Enzymol (1987) 5.08
Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene. Mol Cell Biol (1987) 5.05
The Nottingham Health Profile: subjective health status and medical consultations. Soc Sci Med A (1981) 5.02
Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harb Symp Quant Biol (1975) 4.87
Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter. Genes Dev (1992) 4.85
Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter. Proc Natl Acad Sci U S A (1988) 4.83
T antigen repression of SV40 early transcription from two promoters. Cell (1981) 4.83
Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID. Proc Natl Acad Sci U S A (1990) 4.79
Separation and characterization of factors mediating accurate transcription by RNA polymerase II. J Biol Chem (1982) 4.71
Influence of substrate wettability on the attachment of marine bacteria to various surfaces. Appl Microbiol (1975) 4.70
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Splicing of adenovirus RNA in a cell-free transcription system. Proc Natl Acad Sci U S A (1983) 4.69
Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor. Cell (1991) 4.66
Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. Proc Natl Acad Sci U S A (1986) 4.59
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev (1989) 4.53
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. Cell (1983) 4.42
Expression of early adenovirus genes requires a viral encoded acidic polypeptide. Proc Natl Acad Sci U S A (1981) 4.42
The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element. Nature (1982) 4.42
Host-range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology (1977) 4.40
Inhibition of p53 transactivation required for transformation by adenovirus early 1B protein. Nature (1992) 4.36
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HIV-1 Tat protein promotes formation of more-processive elongation complexes. EMBO J (1991) 4.18
Cloning of a transcriptionally active human TATA binding factor. Science (1990) 4.18
The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains. Genes Dev (1988) 4.14
Photoactivated gamma-secretase inhibitors directed to the active site covalently label presenilin 1. Nature (2000) 4.12
In vitro transcription: whole-cell extract. Methods Enzymol (1983) 4.07
Affinity chromatography of splicing complexes: U2, U5, and U4 + U6 small nuclear ribonucleoprotein particles in the spliceosome. Science (1986) 4.01
Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate. Cell (1984) 4.00
Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells. Proc Natl Acad Sci U S A (1985) 3.95
An improved bacteriophage lambda vector: construction of model recombinants coding for kanamycin resistance. Gene (1977) 3.94
Function of a yeast TATA element-binding protein in a mammalian transcription system. Nature (1988) 3.91
Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol (1975) 3.87
RNA polymerase interaction at the promoter--operator region of the tryptophan operon of Escherichia coli and Salmonella typhimurium. J Mol Biol (1978) 3.84
Transcription maps of adenovirus. Methods Enzymol (1980) 3.82