Published in Science on September 19, 1986
Exon definition may facilitate splice site selection in RNAs with multiple exons. Mol Cell Biol (1990) 8.17
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor. Mol Cell Biol (1986) 7.49
A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA. EMBO J (1987) 6.26
Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor. Proc Natl Acad Sci U S A (1989) 5.11
Transcription-dependent colocalization of the U1, U2, U4/U6, and U5 snRNPs in coiled bodies. J Cell Biol (1992) 4.57
An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly. EMBO J (1987) 4.52
A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site. EMBO J (1988) 4.34
Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles. Mol Cell Biol (1988) 3.56
Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein. Proc Natl Acad Sci U S A (1988) 3.38
A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction. EMBO J (1994) 3.17
Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes. Mol Cell Biol (1987) 3.00
Differential binding of heterogeneous nuclear ribonucleoproteins to mRNA precursors prior to spliceosome assembly in vitro. Mol Cell Biol (1992) 2.66
Early commitment of yeast pre-mRNA to the spliceosome pathway. Mol Cell Biol (1988) 2.64
Protein composition of mammalian spliceosomes assembled in vitro. Proc Natl Acad Sci U S A (1990) 2.58
Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA. Proc Natl Acad Sci U S A (1989) 2.34
20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition. Proc Natl Acad Sci U S A (1989) 2.33
Evidence for a base-pairing interaction between U6 small nuclear RNA and 5' splice site during the splicing reaction in yeast. Proc Natl Acad Sci U S A (1992) 2.28
Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly. Mol Cell Biol (1994) 2.03
The spliceosomal snRNAs of Caenorhabditis elegans. Nucleic Acids Res (1990) 2.03
RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus. Mol Cell Biol (1988) 2.01
The iron-responsive element binding protein: a method for the affinity purification of a regulatory RNA-binding protein. Proc Natl Acad Sci U S A (1989) 1.95
The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome. Proc Natl Acad Sci U S A (1989) 1.87
Identification of a functional mammalian spliceosome containing unspliced pre-mRNA. Proc Natl Acad Sci U S A (1988) 1.85
U1 small nuclear ribonucleoproteins are required early during spliceosome assembly. Mol Cell Biol (1987) 1.83
Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae. Nucleic Acids Res (1990) 1.80
Identification of a complex associated with processing and polyadenylation in vitro of herpes simplex virus type 1 thymidine kinase precursor RNA. Mol Cell Biol (1987) 1.79
Tissue-specific expression and cDNA cloning of small nuclear ribonucleoprotein-associated polypeptide N. Proc Natl Acad Sci U S A (1988) 1.78
Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts. Nucleic Acids Res (1991) 1.78
The spliceosome assembly pathway in mammalian extracts. Mol Cell Biol (1992) 1.78
A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae. Mol Cell Biol (1993) 1.75
Mapping U2 snRNP--pre-mRNA interactions using biotinylated oligonucleotides made of 2'-OMe RNA. EMBO J (1989) 1.73
The basic domain of Rev from human immunodeficiency virus type 1 specifically blocks the entry of U4/U6.U5 small nuclear ribonucleoprotein in spliceosome assembly. J Virol (1993) 1.65
Pre-mRNA splicing mutants of Schizosaccharomyces pombe. EMBO J (1989) 1.63
Roles of U4 and U6 snRNAs in the assembly of splicing complexes. EMBO J (1992) 1.60
Purification of RNA and RNA-protein complexes by an R17 coat protein affinity method. Nucleic Acids Res (1990) 1.59
Specific accessory sequences in Saccharomyces cerevisiae introns control assembly of pre-mRNAs into spliceosomes. EMBO J (1987) 1.53
A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein. Proc Natl Acad Sci U S A (1990) 1.48
In vitro apolipoprotein B mRNA editing: identification of a 27S editing complex. Proc Natl Acad Sci U S A (1991) 1.47
A high yield affinity purification method for specific RNA-binding proteins: isolation of the iron regulatory factor from human placenta. Nucleic Acids Res (1990) 1.43
Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly. Nucleic Acids Res (1991) 1.40
Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation. EMBO J (1998) 1.38
Assembly of a polyadenylation-specific 25S ribonucleoprotein complex in vitro. Mol Cell Biol (1988) 1.28
Sequence-specific affinity selection of mammalian splicing complexes. Nucleic Acids Res (1990) 1.22
RNase-assisted RNA chromatography. RNA (2010) 1.22
Combinatorial splicing of exon pairs by two-site binding of U1 small nuclear ribonucleoprotein particle. Mol Cell Biol (1991) 1.19
Capping of mammalian U6 small nuclear RNA in vitro is directed by a conserved stem-loop and AUAUAC sequence: conversion of a noncapped RNA into a capped RNA. Mol Cell Biol (1990) 1.19
Mammalian U6 small nuclear RNA undergoes 3' end modifications within the spliceosome. Mol Cell Biol (1993) 1.17
In vivo expression and purification of aptamer-tagged small RNA regulators. Nucleic Acids Res (2009) 1.15
Antisense probes targeted to an internal domain in U2 snRNP specifically inhibit the second step of pre-mRNA splicing. Nucleic Acids Res (1992) 1.12
The nuclear matrix phosphoprotein p255 associates with splicing complexes as part of the [U4/U6.U5] tri-snRNP particle. Nucleic Acids Res (1995) 1.12
Splice site choice in a complex transcription unit containing multiple inefficient polyadenylation signals. Mol Cell Biol (1991) 1.10
A yeast splicing factor is localized in discrete subnuclear domains. EMBO J (1992) 1.08
Role of the branch site/3'-splice site region in adenovirus-2 E1A pre-mRNA alternative splicing: evidence for 5'- and 3'-splice site co-operation. Nucleic Acids Res (1989) 1.06
The pre-mRNA 5' cap determines whether U6 small nuclear RNA succeeds U1 small nuclear ribonucleoprotein particle at 5' splice sites. Mol Cell Biol (1998) 1.05
Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus. Mol Cell Biol (2003) 1.04
An ATP-independent U2 small nuclear ribonucleoprotein particle/precursor mRNA complex requires both splice sites and the polypyrimidine tract. Proc Natl Acad Sci U S A (1992) 1.03
Evidence from complementation assays in vitro that U5 snRNP is required for both steps of mRNA splicing. EMBO J (1989) 1.03
Alterations of RNase H sensitivity of the 3' splice site region during the in vitro splicing reaction. Nucleic Acids Res (1991) 1.03
Retroviral splicing suppressor sequesters a 3' splice site in a 50S aberrant splicing complex. Mol Cell Biol (2005) 1.00
A block in mammalian splicing occurring after formation of large complexes containing U1, U2, U4, U5, and U6 small nuclear ribonucleoproteins. Mol Cell Biol (1989) 0.99
Splice acceptor site for the env message of Moloney murine leukemia virus. J Virol (1987) 0.98
The branchpoint residue is recognized during commitment complex formation before being bulged out of the U2 snRNA-pre-mRNA duplex. Mol Cell Biol (1997) 0.98
Three novel functional variants of human U5 small nuclear RNA. Mol Cell Biol (1992) 0.96
Two spliceosomes can form simultaneously and independently on synthetic double-intron messenger RNA precursors. EMBO J (1987) 0.94
In vitro splicing of fibronectin pre-mRNAs. Nucleic Acids Res (1990) 0.94
An oligodeoxynucleotide affinity column for the isolation of sequence specific DNA binding proteins. Nucleic Acids Res (1988) 0.89
Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing. EMBO J (2001) 0.89
Developmental and tissue-specific expression of U4 small nuclear RNA genes. Mol Cell Biol (1988) 0.88
Differential block of U small nuclear ribonucleoprotein particle interactions during in vitro splicing of adenovirus E1A transcripts containing abnormally short introns. Mol Cell Biol (1991) 0.88
Isolation and characterization of polyadenylation complexes assembled in vitro. RNA (2000) 0.87
Electron microscopic identification of the yeast spliceosome. EMBO J (1988) 0.87
Early steps in the assembly of vesicular stomatitis virus nucleocapsids in infected cells. J Virol (1988) 0.85
A novel 40S multi-snRNP complex isolated from rat liver nuclei. Nucleic Acids Res (1991) 0.81
Factor interactions with the simian virus 40 early pre-mRNA influence branch site selection and alternative splicing. Mol Cell Biol (1989) 0.81
Purified U5 small nuclear ribonucleoprotein can relieve the inhibition of spliceosome assembly and splicing by snRNP-free nuclear proteins. Nucleic Acids Res (1989) 0.81
The 5' end domain of U2 snRNA is required to establish the interaction of U2 snRNP with U2 auxiliary factor(s) during mammalian spliceosome assembly. Nucleic Acids Res (1991) 0.79
Potential role of poly(A) polymerase in the assembly of polyadenylation-specific RNP complexes. Nucleic Acids Res (1991) 0.78
Interplay between U2 snRNP and 3' splice factor(s) for branch point selection on human beta-globin pre-mRNA. Nucleic Acids Res (1990) 0.76
Identifying proteins that bind to specific RNAs - focus on simple repeat expansion diseases. Nucleic Acids Res (2016) 0.76
Translation of globin RNA spliced in vitro. Biochem J (1988) 0.75
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell (1977) 61.24
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry (1973) 43.61
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc Natl Acad Sci U S A (1980) 24.21
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A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature (1986) 14.34
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Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena. Cell (1982) 12.12
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol (1973) 11.40
Structure of the adenovirus 2 early mRNAs. Cell (1978) 10.54
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell (1985) 10.06
Five intermediate complexes in transcription initiation by RNA polymerase II. Cell (1989) 9.22
Recognition of cap structure in splicing in vitro of mRNA precursors. Cell (1984) 8.67
A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes. Nature (1986) 8.64
Speculations on RNA splicing. Cell (1981) 8.28
Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev (1999) 8.21
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell (1986) 8.06
Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA. Cell (1988) 8.00
Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A (1973) 7.75
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Regulation by HIV Rev depends upon recognition of splice sites. Cell (1989) 7.13
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A high-efficiency HeLa cell nuclear transcription extract. DNA (1988) 5.81
Heterogeneous nuclear ribonucleoproteins: role in RNA splicing. Science (1986) 5.52
Accurate cleavage and polyadenylation of exogenous RNA substrate. Cell (1985) 5.27
Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors. Science (1984) 5.11
Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene. Mol Cell Biol (1987) 5.05
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Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev (1989) 4.53
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. Cell (1983) 4.42
In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence. Cell (1981) 4.42
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The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element. Nature (1982) 4.42
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