Published in Gene on May 01, 1977
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A (1979) 72.18
Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning. J Virol (1979) 2.99
Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins. J Bacteriol (1978) 1.63
Bacteriophage lambda and plasmid vectors, allowing fusion of cloned genes in each of the three translational phases. Nucleic Acids Res (1978) 0.94
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell (1977) 61.24
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry (1973) 43.61
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc Natl Acad Sci U S A (1980) 24.21
RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell (2000) 20.32
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol (1972) 19.88
Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A (1978) 18.67
Splicing of messenger RNA precursors. Annu Rev Biochem (1986) 14.41
A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature (1986) 14.34
Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc Natl Acad Sci U S A (1977) 14.34
Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs. Cell (1979) 12.83
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol (1973) 11.40
Structure of the adenovirus 2 early mRNAs. Cell (1978) 10.54
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell (1985) 10.06
Five intermediate complexes in transcription initiation by RNA polymerase II. Cell (1989) 9.22
Recognition of cap structure in splicing in vitro of mRNA precursors. Cell (1984) 8.67
A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes. Nature (1986) 8.64
Speculations on RNA splicing. Cell (1981) 8.28
Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev (1999) 8.21
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell (1986) 8.06
Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA. Cell (1988) 8.00
Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A (1973) 7.75
Splicing of messenger RNA precursors. Science (1987) 7.61
Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J Mol Biol (1972) 7.49
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor. Mol Cell Biol (1986) 7.49
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes. Cell (1987) 7.44
Regulation by HIV Rev depends upon recognition of splice sites. Cell (1989) 7.13
In vitro transcription of adenovirus. J Virol (1981) 7.06
A multicomponent complex is involved in the splicing of messenger RNA precursors. Cell (1985) 6.27
Evolutionary fates and origins of U12-type introns. Mol Cell (1998) 6.25
The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products. Genes Dev (1988) 6.23
Site-specific modification of pre-mRNA: the 2'-hydroxyl groups at the splice sites. Science (1992) 5.87
Human CCAAT-binding proteins have heterologous subunits. Cell (1988) 5.81
A high-efficiency HeLa cell nuclear transcription extract. DNA (1988) 5.81
Heterogeneous nuclear ribonucleoproteins: role in RNA splicing. Science (1986) 5.52
Accurate cleavage and polyadenylation of exogenous RNA substrate. Cell (1985) 5.27
Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors. Science (1984) 5.11
Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene. Mol Cell Biol (1987) 5.05
Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harb Symp Quant Biol (1975) 4.87
Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter. Proc Natl Acad Sci U S A (1988) 4.83
T antigen repression of SV40 early transcription from two promoters. Cell (1981) 4.83
Separation and characterization of factors mediating accurate transcription by RNA polymerase II. J Biol Chem (1982) 4.71
On the origin of RNA splicing and introns. Cell (1985) 4.70
Sequence heterogeneity in closed simian virus 40 deoxyribonucleic acid. J Virol (1972) 4.69
Splicing of adenovirus RNA in a cell-free transcription system. Proc Natl Acad Sci U S A (1983) 4.69
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev (1989) 4.53
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. Cell (1983) 4.42
Expression of early adenovirus genes requires a viral encoded acidic polypeptide. Proc Natl Acad Sci U S A (1981) 4.42
The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element. Nature (1982) 4.42
Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol (1974) 4.30
HIV-1 Tat protein promotes formation of more-processive elongation complexes. EMBO J (1991) 4.18
The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains. Genes Dev (1988) 4.14
In vitro transcription: whole-cell extract. Methods Enzymol (1983) 4.07
Nucleotide sequence and formation of the transforming gene of a mouse sarcoma virus. Nature (1981) 4.05
Affinity chromatography of splicing complexes: U2, U5, and U4 + U6 small nuclear ribonucleoprotein particles in the spliceosome. Science (1986) 4.01
Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate. Cell (1984) 4.00
Function of a yeast TATA element-binding protein in a mammalian transcription system. Nature (1988) 3.91
Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol (1975) 3.87
Transcription maps of adenovirus. Methods Enzymol (1980) 3.82
DNA topology and a minimal set of basal factors for transcription by RNA polymerase II. Cell (1993) 3.77
RNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor. Proc Natl Acad Sci U S A (1979) 3.75
Site-specific polyadenylation in a cell-free reaction. Cell (1984) 3.59
HIV-1 Tat protein trans-activates transcription in vitro. Cell (1990) 3.57
Characterization of cDNAs encoding the polypyrimidine tract-binding protein. Genes Dev (1991) 3.55
SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen. Gene (1981) 3.54
Interactions between RNA polymerase II, factors, and template leading to accurate transcription. J Biol Chem (1984) 3.47
Analysis of a 5' leader sequence on murine leukemia virus 21S RNA: heteroduplex mapping with long reverse transcriptase products. Cell (1978) 3.46
Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc Natl Acad Sci U S A (1991) 3.43
The octamer-binding proteins form multi-protein--DNA complexes with the HSV alpha TIF regulatory protein. EMBO J (1989) 3.42
Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein. Proc Natl Acad Sci U S A (1988) 3.38
Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing. Nature (1993) 3.36
Generation of adenovirus by transfection of plasmids. Nucleic Acids Res (1983) 3.33
A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. Proc Natl Acad Sci U S A (1996) 3.25
Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences. Proc Natl Acad Sci U S A (1989) 3.18
Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions. Cell (1989) 3.18
Regulation of heat shock protein 70 gene expression by c-myc. Nature (1984) 3.18
Nucleotide sequence and evolution of a mammalian alpha-tubulin messenger RNA. J Mol Biol (1981) 3.18
Characterization of the branch site in lariat RNAs produced by splicing of mRNA precursors. Nature (1985) 3.17
Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary dna gene. J Mol Biol (1982) 3.17
The infectivity of adenovirus 5 DNA-protein complex. Virology (1976) 3.12
Structural analysis of the interaction between the human immunodeficiency virus Rev protein and the Rev response element. Proc Natl Acad Sci U S A (1991) 3.06
Transcription of simian virus 40. 3. Mapping of "early" and "late" species of RNA. Proc Natl Acad Sci U S A (1973) 3.04
A coactivator of pre-mRNA splicing. Genes Dev (1998) 3.04
Association of nuclear matrix antigens with exon-containing splicing complexes. J Cell Biol (1994) 3.01
Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif. Science (1988) 3.01
A mutational analysis of spliceosome assembly: evidence for splice site collaboration during spliceosome formation. Genes Dev (1987) 2.99
Comparison of viral RNA sequences in adenovirus 2-transformed and lytically infected cells. J Mol Biol (1975) 2.95
The p50 subunit of NF-kappa B associates with the NF-IL6 transcription factor. Proc Natl Acad Sci U S A (1992) 2.93
Identification and characterization of a HeLa nuclear protein that specifically binds to the trans-activation-response (TAR) element of human immunodeficiency virus. Proc Natl Acad Sci U S A (1990) 2.92
A helix-loop-helix protein related to the immunoglobulin E box-binding proteins. Mol Cell Biol (1990) 2.90
Distinct factors bind to apparently homologous sequences in the immunoglobulin heavy-chain enhancer. Nature (1986) 2.86
RNA synthesis in isolated nuclei: identification and comparison of adenovirus 2 encoded transcripts synthesized in vitro and vivo. J Mol Biol (1979) 2.86
Linkage between immunoglobulin E responses underlying asthma and rhinitis and chromosome 11q. Lancet (1989) 2.85
A yeast and a human CCAAT-binding protein have heterologous subunits that are functionally interchangeable. Cell (1988) 2.84
Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens. Mol Cell Biol (1986) 2.81
Interactions of the Oct-1 POU subdomains with specific DNA sequences and with the HSV alpha-trans-activator protein. Genes Dev (1990) 2.80
Structure of late adenovirus 2 heterogeneous nuclear RNA. J Mol Biol (1979) 2.77
Cloning and nucleotide sequence of DNA coding for bovine preproparathyroid hormone. Proc Natl Acad Sci U S A (1979) 2.72
Physical mapping of temperature-sensitive mutations of adenoviruses. J Mol Biol (1975) 2.69
5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole inhibits transcription elongation by RNA polymerase II in vitro. J Biol Chem (1989) 2.65
Viral DNA sequences in cells transformed by simian virus 40, adenovirus type 2 and adenovirus type 5. Cold Spring Harb Symp Quant Biol (1975) 2.65