Published in Nat Protoc on January 01, 2008
Silencing of microRNA families by seed-targeting tiny LNAs. Nat Genet (2011) 4.81
Exploiting and antagonizing microRNA regulation for therapeutic and experimental applications. Nat Rev Genet (2009) 3.09
Inhibition of microRNA function by antimiR oligonucleotides. Silence (2012) 2.02
MicroRNA-29c is a signature microRNA under high glucose conditions that targets Sprouty homolog 1, and its in vivo knockdown prevents progression of diabetic nephropathy. J Biol Chem (2011) 1.68
MicroRNA-98 and let-7 confer cholangiocyte expression of cytokine-inducible Src homology 2-containing protein in response to microbial challenge. J Immunol (2009) 1.57
Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Nat Struct Mol Biol (2010) 1.54
Retracted Chromosome 21-derived microRNAs provide an etiological basis for aberrant protein expression in human Down syndrome brains. J Biol Chem (2009) 1.53
microRNA miR-275 is indispensable for blood digestion and egg development in the mosquito Aedes aegypti. Proc Natl Acad Sci U S A (2010) 1.43
MicroRNAs in ovarian carcinomas. Endocr Relat Cancer (2010) 1.25
On measuring miRNAs after transient transfection of mimics or antisense inhibitors. PLoS One (2013) 1.24
A single anti-microRNA antisense oligodeoxyribonucleotide (AMO) targeting multiple microRNAs offers an improved approach for microRNA interference. Nucleic Acids Res (2009) 1.23
Insect microRNAs: biogenesis, expression profiling and biological functions. Insect Biochem Mol Biol (2012) 1.15
Sequence-non-specific effects of RNA interference triggers and microRNA regulators. Nucleic Acids Res (2009) 1.11
Drosophila miR-14 regulates insulin production and metabolism through its target, sugarbabe. Genes Dev (2010) 1.10
Use of target protector morpholinos to analyze the physiological roles of specific miRNA-mRNA pairs in vivo. Nat Protoc (2011) 1.07
MicroRNAs in atherosclerosis and lipoprotein metabolism. Curr Opin Endocrinol Diabetes Obes (2010) 1.03
MicroRNA fate upon targeting with anti-miRNA oligonucleotides as revealed by an improved Northern-blot-based method for miRNA detection. RNA (2011) 1.02
microRNAs: a role in drug resistance in parasitic nematodes? Trends Parasitol (2010) 0.95
Specificity and functionality of microRNA inhibitors. Silence (2010) 0.94
Invoking the power of thrombospondins: regulation of thrombospondins expression. Matrix Biol (2014) 0.92
Gene regulation in Giardia lambia involves a putative microRNA derived from a small nucleolar RNA. PLoS Negl Trop Dis (2011) 0.91
The profile of snoRNA-derived microRNAs that regulate expression of variant surface proteins in Giardia lamblia. Cell Microbiol (2012) 0.90
High-throughput experimental studies to identify miRNA targets directly, with special focus on the mammalian brain. Brain Res (2010) 0.86
In vitro optimization of 2'-OMe-4'-thioribonucleoside-modified anti-microRNA oligonucleotides and its targeting delivery to mouse liver using a liposomal nanoparticle. Nucleic Acids Res (2013) 0.85
Overexpression of miR-125a in myelodysplastic syndrome CD34+ cells modulates NF-κB activation and enhances erythroid differentiation arrest. PLoS One (2014) 0.83
FDF-PAGE: a powerful technique revealing previously undetected small RNAs sequestered by complementary transcripts. Nucleic Acids Res (2015) 0.83
TRPV4 as a therapeutic target for joint diseases. Naunyn Schmiedebergs Arch Pharmacol (2014) 0.82
Transition of a microRNA from repressing to activating translation depending on the extent of base pairing with the target. PLoS One (2013) 0.82
The long noncoding RNA NRF regulates programmed necrosis and myocardial injury during ischemia and reperfusion by targeting miR-873. Cell Death Differ (2016) 0.78
Role of cancer stem cells in racial disparity in colorectal cancer. Cancer Med (2016) 0.78
A novel member of the let-7 microRNA family is associated with developmental transitions in filarial nematode parasites. BMC Genomics (2015) 0.78
Efficient and specific inhibition of plant microRNA function by anti-microRNA oligonucleotides (AMOs) in vitro and in vivo. Plant Cell Rep (2016) 0.76
Developmental attenuation of N-methyl-D-aspartate receptor subunit expression by microRNAs. Neural Dev (2015) 0.75
Challenge and promise: the role of miRNA for pathogenesis and progression of malignant melanoma. Clin Epigenetics (2010) 0.75
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell (2005) 96.87
Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res (2003) 77.89
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell (1993) 64.62
Prediction of mammalian microRNA targets. Cell (2003) 53.70
Combinatorial microRNA target predictions. Nat Genet (2005) 50.48
Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature (2005) 48.95
Identification of novel genes coding for small expressed RNAs. Science (2001) 44.44
An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science (2001) 44.16
A microRNA polycistron as a potential human oncogene. Nature (2005) 40.21
miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res (2006) 39.25
Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature (2001) 38.75
The nuclear RNase III Drosha initiates microRNA processing. Nature (2003) 35.49
A mammalian microRNA expression atlas based on small RNA library sequencing. Cell (2007) 34.03
Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell (1993) 33.79
Silencing of microRNAs in vivo with 'antagomirs'. Nature (2005) 32.56
A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science (2001) 31.33
MicroRNAs modulate hematopoietic lineage differentiation. Science (2003) 30.05
Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell (2001) 25.86
Principles of microRNA-target recognition. PLoS Biol (2005) 23.70
A microRNA in a multiple-turnover RNAi enzyme complex. Science (2002) 23.36
Asymmetry in the assembly of the RNAi enzyme complex. Cell (2003) 22.18
Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev (2001) 21.82
Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature (2007) 21.53
Specificity of microRNA target selection in translational repression. Genes Dev (2004) 21.14
MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res (2005) 18.58
The role of site accessibility in microRNA target recognition. Nat Genet (2007) 18.52
MicroRNA-directed cleavage of HOXB8 mRNA. Science (2004) 18.33
The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation. Dev Biol (1999) 17.82
Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis. Nature (2005) 17.17
Switching from repression to activation: microRNAs can up-regulate translation. Science (2007) 17.00
Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA. Science (2005) 16.84
Requirement of bic/microRNA-155 for normal immune function. Science (2007) 16.24
siRNAs can function as miRNAs. Genes Dev (2003) 16.08
Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation. Cell (2005) 15.26
Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans. Cell (2006) 15.12
A pancreatic islet-specific microRNA regulates insulin secretion. Nature (2004) 15.02
Endogenous human microRNAs that suppress breast cancer metastasis. Nature (2008) 14.37
Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes. RNA (2005) 14.14
MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods (2007) 13.94
Micromanagers of gene expression: the potentially widespread influence of metazoan microRNAs. Nat Rev Genet (2004) 12.97
Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs. Nature (2004) 12.75
Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2. Cell (2007) 12.72
Disrupting the pairing between let-7 and Hmga2 enhances oncogenic transformation. Science (2007) 12.42
miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting. Cell Metab (2006) 12.41
Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Cell (2008) 12.29
The small RNA profile during Drosophila melanogaster development. Dev Cell (2003) 11.46
LNA-mediated microRNA silencing in non-human primates. Nature (2008) 11.45
MicroRNAs regulate brain morphogenesis in zebrafish. Science (2005) 11.16
A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Cell (2006) 11.01
microRNA target predictions in animals. Nat Genet (2006) 10.76
Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol Cell (2002) 10.30
The tumor suppressor microRNA let-7 represses the HMGA2 oncogene. Genes Dev (2007) 10.15
Intronic microRNA precursors that bypass Drosha processing. Nature (2007) 9.65
Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc Natl Acad Sci U S A (2000) 9.02
Gene targeting by homologous recombination in Drosophila. Science (2000) 8.63
A cellular microRNA mediates antiviral defense in human cells. Science (2005) 8.48
Coaxial stacking of helixes enhances binding of oligoribonucleotides and improves predictions of RNA folding. Proc Natl Acad Sci U S A (1994) 8.06
Sequence-specific inhibition of small RNA function. PLoS Biol (2004) 7.98
Stem cell division is regulated by the microRNA pathway. Nature (2005) 7.79
Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. RNA (2004) 7.71
The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila. Cell (2007) 7.70
Kinetic analysis of the RNAi enzyme complex. Nat Struct Mol Biol (2004) 7.26
Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein. PLoS Biol (2005) 7.23
Production of homozygous mutant ES cells with a single targeting construct. Mol Cell Biol (1992) 7.23
Interferon modulation of cellular microRNAs as an antiviral mechanism. Nature (2007) 7.04
p53-mediated activation of miRNA34 candidate tumor-suppressor genes. Curr Biol (2007) 6.87
Computational identification of microRNA targets. Dev Biol (2004) 6.84
MicroRNA-143 regulates adipocyte differentiation. J Biol Chem (2004) 6.78
microRNA target predictions across seven Drosophila species and comparison to mammalian targets. PLoS Comput Biol (2005) 6.77
Diversity of microRNAs in human and chimpanzee brain. Nat Genet (2006) 6.57
Characterization of microRNA expression levels and their biological correlates in human cancer cell lines. Cancer Res (2007) 6.57
Processing of intronic microRNAs. EMBO J (2007) 6.32
MicroRNA-9a ensures the precise specification of sensory organ precursors in Drosophila. Genes Dev (2006) 6.30
The let-7 MicroRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans. Dev Cell (2005) 5.90
Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver. Nucleic Acids Res (2007) 5.67
Most Caenorhabditis elegans microRNAs are individually not essential for development or viability. PLoS Genet (2007) 5.33
Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by dicer-1. Cell (2007) 5.26
Molecular basis for target RNA recognition and cleavage by human RISC. Cell (2007) 4.40
Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles during larval growth. Genes Dev (2005) 4.34
The microRNA miR-181 targets the homeobox protein Hox-A11 during mammalian myoblast differentiation. Nat Cell Biol (2006) 4.31
Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes. Nucleic Acids Res (2004) 3.97
Antisense-mediated depletion reveals essential and specific functions of microRNAs in Drosophila development. Cell (2005) 3.95
Specificity, duplex degradation and subcellular localization of antagomirs. Nucleic Acids Res (2007) 3.89
RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus. Curr Biol (2005) 3.65
LNA-modified oligonucleotides mediate specific inhibition of microRNA function. Gene (2006) 3.49
Improved targeting of miRNA with antisense oligonucleotides. Nucleic Acids Res (2006) 3.43
Depletion of human micro-RNA miR-125b reveals that it is critical for the proliferation of differentiated cells but not for the down-regulation of putative targets during differentiation. J Biol Chem (2005) 3.33
Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene. Nature (1986) 3.30
Systematic identification of microRNA functions by combining target prediction and expression profiling. Nucleic Acids Res (2006) 3.29
Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides. Nucleic Acids Res (1987) 3.24
Apoptosis induction by antisense oligonucleotides against miR-17-5p and miR-20a in lung cancers overexpressing miR-17-92. Oncogene (2007) 3.11
Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development. PLoS Biol (2007) 3.04
MicroRNA-21 knockdown disrupts glioma growth in vivo and displays synergistic cytotoxicity with neural precursor cell delivered S-TRAIL in human gliomas. Cancer Res (2007) 2.90
MicroRNA expression profiles for the NCI-60 cancer cell panel. Mol Cancer Ther (2007) 2.53
Comparison of siRNA-induced off-target RNA and protein effects. RNA (2007) 2.22
Developmental defects by antisense-mediated inactivation of micro-RNAs 2 and 13 in Drosophila and the identification of putative target genes. Nucleic Acids Res (2003) 2.13
Inhibition of microRNA with antisense oligonucleotides. Methods (2008) 1.93
miR-122 targeting with LNA/2'-O-methyl oligonucleotide mixmers, peptide nucleic acids (PNA), and PNA-peptide conjugates. RNA (2007) 1.90
Double-stranded regions are essential design components of potent inhibitors of RISC function. RNA (2007) 1.83
Cholesteryl-conjugated oligonucleotides: synthesis, properties, and activity as inhibitors of replication of human immunodeficiency virus in cell culture. Proc Natl Acad Sci U S A (1989) 1.71
A microRNA in a multiple-turnover RNAi enzyme complex. Science (2002) 23.36
Asymmetry in the assembly of the RNAi enzyme complex. Cell (2003) 22.18
Small silencing RNAs: an expanding universe. Nat Rev Genet (2009) 13.88
A distinct small RNA pathway silences selfish genetic elements in the germline. Science (2006) 11.63
A biochemical framework for RNA silencing in plants. Genes Dev (2003) 9.95
Perspective: machines for RNAi. Genes Dev (2005) 9.37
Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. Cell (2005) 9.35
Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells. Science (2008) 8.62
microPrimer: the biogenesis and function of microRNA. Development (2005) 8.19
Sequence-specific inhibition of small RNA function. PLoS Biol (2004) 7.98
A protein sensor for siRNA asymmetry. Science (2004) 7.77
Kinetic analysis of the RNAi enzyme complex. Nat Struct Mol Biol (2004) 7.26
Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein. PLoS Biol (2005) 7.23
Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways. Mol Cell (2002) 6.43
MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5' region. EMBO J (2004) 6.27
RNAi: nature abhors a double-strand. Curr Opin Genet Dev (2002) 5.74
The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC. Curr Biol (2007) 5.35
Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by dicer-1. Cell (2007) 5.26
Paternally induced transgenerational environmental reprogramming of metabolic gene expression in mammals. Cell (2010) 5.22
RISC assembly defects in the Drosophila RNAi mutant armitage. Cell (2004) 4.88
Sorting of Drosophila small silencing RNAs. Cell (2007) 4.67
Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. Cell (2009) 4.39
Modular recognition of RNA by a human pumilio-homology domain. Cell (2002) 4.17
Argonaute loading improves the 5' precision of both MicroRNAs and their miRNA* strands in flies. Curr Biol (2008) 4.07
Target RNA-directed trimming and tailing of small silencing RNAs. Science (2010) 3.98
Diversifying microRNA sequence and function. Nat Rev Mol Cell Biol (2013) 3.80
The RNA-induced silencing complex is a Mg2+-dependent endonuclease. Curr Biol (2004) 3.68
Designing siRNA that distinguish between genes that differ by a single nucleotide. PLoS Genet (2006) 3.23
Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway. RNA (2009) 3.19
The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters. Cell (2009) 3.13
MicroRNA biogenesis: drosha can't cut it without a partner. Curr Biol (2005) 2.85
Deep annotation of Drosophila melanogaster microRNAs yields insights into their processing, modification, and emergence. Genome Res (2010) 2.56
Why do miRNAs live in the miRNP? Genes Dev (2002) 2.41
An ancient transcription factor initiates the burst of piRNA production during early meiosis in mouse testes. Mol Cell (2013) 2.23
In vitro analysis of RNA interference in Drosophila melanogaster. Methods (2003) 2.20
Five siRNAs targeting three SNPs may provide therapy for three-quarters of Huntington's disease patients. Curr Biol (2009) 2.19
Argonaute divides its RNA guide into domains with distinct functions and RNA-binding properties. Cell (2012) 2.07
Selective silencing by RNAi of a dominant allele that causes amyotrophic lateral sclerosis. Aging Cell (2003) 1.88
Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains. Mol Cell (2011) 1.79
Adaptation to P element transposon invasion in Drosophila melanogaster. Cell (2011) 1.76
A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe. Genes Dev (2004) 1.69
Long-term, efficient inhibition of microRNA function in mice using rAAV vectors. Nat Methods (2012) 1.67
The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1. Curr Biol (2011) 1.66
Beginning to understand microRNA function. Cell Res (2007) 1.59
Argonaute protein identity and pairing geometry determine cooperativity in mammalian RNA silencing. RNA (2011) 1.59
Dicer partner proteins tune the length of mature miRNAs in flies and mammals. Cell (2012) 1.57
A role for microRNAs in the Drosophila circadian clock. Genes Dev (2009) 1.56
UAP56 couples piRNA clusters to the perinuclear transposon silencing machinery. Cell (2012) 1.53
MicroRNAs: regulating a change of heart. Circulation (2009) 1.37
Phosphate and R2D2 restrict the substrate specificity of Dicer-2, an ATP-driven ribonuclease. Mol Cell (2011) 1.36
MicroRNA-regulated, systemically delivered rAAV9: a step closer to CNS-restricted transgene expression. Mol Ther (2010) 1.30
Rethinking the microprocessor. Cell (2006) 1.29
Argonaute proteins. Curr Biol (2011) 1.26
Linking SNPs to CAG repeat length in Huntington's disease patients. Nat Methods (2008) 1.19
Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins. RNA (2010) 1.19
Small silencing RNAs. Curr Biol (2007) 1.18
RNA silencing: genomic defence with a slice of pi. Nature (2007) 1.12
A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation. Silence (2011) 1.08
Sustained miRNA-mediated knockdown of mutant AAT with simultaneous augmentation of wild-type AAT has minimal effect on global liver miRNA profiles. Mol Ther (2012) 1.07
Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection. Silence (2012) 1.07
MicroRNA-33-dependent regulation of macrophage metabolism directs immune cell polarization in atherosclerosis. J Clin Invest (2015) 1.03
Rapid and specific purification of Argonaute-small RNA complexes from crude cell lysates. RNA (2012) 1.00
Cnidarian microRNAs frequently regulate targets by cleavage. Genome Res (2014) 0.96
Antisense piRNA amplification, but not piRNA production or nuage assembly, requires the Tudor-domain protein Qin. EMBO J (2014) 0.91
Biochemical dissection of RNA silencing in plants. Methods Mol Biol (2004) 0.91
Huntington's disease: silencing a brutal killer. Exp Neurol (2009) 0.89
Small RNA-directed silencing: the fly finds its inner fission yeast? Curr Biol (2013) 0.84
Inorganic phosphate blocks binding of pre-miRNA to Dicer-2 via its PAZ domain. EMBO J (2014) 0.84
SnapShot: Fly piRNAs, PIWI proteins, and the ping-pong cycle. Cell (2009) 0.81
Defining piRNA primary transcripts. Cell Cycle (2013) 0.80
SnapShot: mouse piRNAs, PIWI proteins, and the ping-pong cycle. Cell (2009) 0.78
siRNAs knock down hepatitis. Nat Med (2003) 0.78
Nucleus and gene expression. Curr Opin Cell Biol (2009) 0.75
Single-Molecule Imaging Reveals that Argonaute Reshapes the Binding Properties of Its Nucleic Acid Guides. Cell (2016) 0.75